CN101502548A - Method for processing cooked notoginseng - Google Patents
Method for processing cooked notoginseng Download PDFInfo
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Abstract
The invention relates to a concocting method of cooked panax notoginseng, comprising the following steps of taking raw panax notoginseng or thoroughly moistened panax notoginseng; or raw panax notoginseng pieces or thoroughly moistened panax notoginseng pieces; or panax notoginseng powder; arranging in a flowing steam kettle for steaming for 1-15 hours at high temperature of 106-125 DEG C and high pressure of 0.03-0.14MPa, taking out till the interior and the exterior are dark brown and drying. The cooked panax notoginseng concocted by the method has stable quality, long storage time, significant blood enrichment effect and improves medicinal value of the panax notoginseng.
Description
Technical field
The present invention relates to field of medicaments, specifically, relate to a kind of concocting method of cooked Radix Notoginseng.
Background technology
Radix Notoginseng is clinical Chinese crude drug commonly used, medicinal efficacy difference before and after concocting.
1, Radix Notoginseng has the saying of " give birth to and drive ripe benefit away " from ancient times.
Radix Notoginseng beginning is stated from the Compendium of Material Medica, Li Shizhen (1518-1593 A.D.) say " [abnormal smells from the patient] sweet, little hardship, temperature, nontoxic.[curing mainly] hemostasis, the blood that looses, analgesic therapy.", since then, most of books on Chinese herbal medicine of successive dynasties are all put down in writing Radix Notoginseng for controlling the panacea of traumatic injury knife injury, traumatic hemorrhage, and Chinese Pharmacopoeia even to this day also records and is dissipating blood stasis hemostasis, subduing swelling and relieving pain.Be used for spitting of blood, spit blood, epistaxis is had blood in stool, metrorrhagia, traumatic hemorrhage, breast ventral spine pain, tumbling and swelling.The Compendium of Material Medica record: Radix Notoginseng, all canes are pounced on trauma, and the dripping person of blood stasis chews promptly ending of mashed net for catching birds or fish immediately, and bruise the person promptly dissipate, and puerperal, clothes were also good so can control all mass formed by blood stasis." book on Chinese herbal medicine newly organized " record: Radix Notoginseng, the refreshing medicine of hemostasis also, the blood of upper, middle and lower no matter, all have an outer person more, privately simply also imitates, adding is enriched blood, then more refreshing in the Qi-tonifying drug." beautiful Chinese catalpa medicine is separated " record: Radix Notoginseng and battalion hemostasis, the clots absorbing of promoting blood circulation, clots absorbing blood and sword fresh blood, all puerperal, menstrual period, traumatic injury, carbuncle, all blood stasis are all broken, and all nosebleeds, metrorrhagia, knife injury, arrow of telling are shot wounded, and all fresh blood all end." book on Chinese herbal medicine converges and says " then thought: Radix Notoginseng, property is flat, and is nontoxic.Supplementary Amplifications of the Compendium of Materia Medica record: ginseng qi-tonifying the first, Radix Notoginseng enrich blood the first, flavor with and merit also waits, be the most precious person of Chinese medicine." in the medical inner feelings ginseng west record " record: Radix Notoginseng has blood stasis dispelling and does not hinder the effect of fresh blood." Japan hanako materia medica " claims: the Radix Notoginseng promoting tissue regeneration by removing blood stasis, subduing swelling and relieving pain can stop blooding and not stay blood stasis, promoting the circulation of blood and do not hinder fresh blood." book on Chinese herbal medicine is looked for the truth " meaning: Radix Notoginseng, bitter in the mouth are slightly cold and temperature, so all golden angle of throat arrows are hindered, and fall and pounce on flogging injury blood and go out incessantly, chew and mashedly be coated with it, promptly end, and to tell nosebleed, the hematochezia nosebleed, hematochezia blood stasis, metrorrhagia incessant menorrhea, lochiostasis, tiger stings that snakebite is hemorrhage to be controlled, and this is the medicine of the cloudy blood system of positive bright fault, so can control all disorders of blood." Chinese Medicine voluminous dictionary " concludes the Radix Notoginseng effect of forefathers' research: the Radix Notoginseng function is enriched blood, blood stasis removing blood, and the hemostasis nosebleed can be led to and can be mended, and effect is the best, is the most precious person of Chinese medicine.Radix Notoginseng eats something rare eliminating blood stasis to promote regeneration of blood, reducing swelling and alleviating pain, and have hemostasis not stay blood stasis, and promoting the circulation of blood and do not hinder the advantage of fresh blood, but ripe clothes tonification is healthy and strong.Record of Pharmacopoeia of the People's Republic of China version in 2005: Radix Notoginseng, the dissipating blood stasis hemostasis, subduing swelling and relieving pain is used for spitting of blood, spits blood, and epistaxis is had blood in stool, metrorrhagia, traumatic hemorrhage, breast ventral spine pain, tumbling and swelling.
Be the document record of blood circulation promoting and blood stasis dispelling function more than for Radix Notoginseng " gives birth to and drives away ".
" 85 years versions of Guangxi Chinese medicine processing standard " recorded: Radix Notoginseng gives birth to product: dissipating blood stasis hemostasis, subduing swelling and relieving pain, ripe product: benefiting blood and regulating blood circulation." 86 years versions of Yunnan Province's prepared slices of Chinese crude drugs concocted specification " record: ", think that ' give birth to and drive ripe benefit away ' is so ripe usefulness is enriched blood strong tonify deficiency according to our province medication custom." " Ministry of Health of the People's Republic of China's ministerial standard---Chinese traditional patent formulation preparation " 12 record " cooked Radix Notoginseng sheet (WS3-B-2449-97) " function with cure mainly: benefiting blood and regulating blood circulation.Be used for anemia, the weakness of losing blood, menoxenia, puerperal stagnant blood not to the utmost." national standard for traditional Chinese medicines compilation surgery gynecological fascicle " records " cooked Radix Notoginseng loose WS-11219 (ZD-1219)-2002 " function and cures mainly: benefiting blood and regulating blood circulation.Be used for anemia, the weakness of losing blood, menoxenia." national standard for traditional Chinese medicines compilation internal medicine qi-blood-body fluid fascicle " records " cooked Radix Notoginseng ball WS-10670 (ZD-0670)-2002 " function and cures mainly: benefiting blood and regulating blood circulation.Be used for anemia, the weakness of losing blood.
More than these documents and materials put down in writing the effect of cooked Radix Notoginseng tool blood yiqi, i.e. " ripe benefit ".
2, the process of preparing Chinese medicine evolution of Radix Notoginseng
According to going through an edition Chinese Pharmacopoeia, codes such as the version record in 1988 of national prepared slices of Chinese crude drugs concocted specification, the processed product specification of Radix Notoginseng has Radix Notoginseng powder, Radix Notoginseng Tabellae, and the process of preparing Chinese medicine specification and the method that do not have cooked Radix Notoginseng sheet, cooked Radix Notoginseng powder to concoct are recorded.
" Guangxi Chinese medicine processing standard " (version in 1985) recorded: Radix Notoginseng gives birth to product: dissipating blood stasis hemostasis, subduing swelling and relieving pain, ripe product: benefiting blood and regulating blood circulation.Process of preparing Chinese medicine specification has: give birth to four kinds of product, Radix Notoginseng powder, Radix Notoginseng Tabellae, cooked Radix Notoginseng.
" Yunnan Province's prepared slices of Chinese crude drugs concocted specification " (version in 1986) recorded the Radix Notoginseng function: dissipating blood stasis, hemostasis, detumescence, analgesic therapy." annotate:, think that ' give birth to and drive ripe benefit away ' is so ripe usefulness is enriched blood strong tonify deficiency according to our province medication custom in addition." the decoction pieces specification: fecula, ripe powder, steam three kinds in powder.
Above Guangxi, Yunnan two provinces (district) concocted specification are all shown the processed product specification of cooked Radix Notoginseng, but do not have concocting method.
In the cooked Radix Notoginseng concocting method, bibliographical information mainly contains fried method and steams two kinds of methods, there are some researches show fried method content of the total saponins in radix notoginseng 60%～70% of the product of only making a living, and along with the intensification of fried degree, total saponin content sharply descends, and it is not proper that therefore fried method is concocted Radix Notoginseng, see " improvement of cooked Radix Notoginseng concocting method " literary composition [Gai Xue etc., " Shandong medicine thing ", 2005,24 (5): 304].
Open fringe report [" research of cooked Radix Notoginseng concocting method ", " Chinese patent medicine ", 1989,11 (11): 20], the raw sangqi ginseng sheet: get Radix Notoginseng and clean, run through, be cut into 0.1cm thickness sheet, dry.Steam Radix Notoginseng: get above-mentioned raw sangqi ginseng sheet portion, put in the food steamer and steam to well-done, taking-up is dried.Fried Radix Notoginseng Tabellae: get above-mentioned raw sangqi ginseng sheet portion, sallow with the fried surface of Oleum Camelliae, take out, cool.
Gai Xue etc. report [" improvement of cooked Radix Notoginseng concocting method ", " Shandong medicine thing ", 2005,24 (5): 304], raw sangqi ginseng: get clean Radix Notoginseng, smash to pieces, porphyrize powder (crossing sieve No. three, down together).Cooked Radix Notoginseng 1: get clean Radix Notoginseng, clean, steam 6h, take out section, drying to saturating.The porphyrize powder.Cooked Radix Notoginseng 2: get clean Radix Notoginseng, clean, directly high pressure steams 45min, to saturating, takes out, and cools section, drying, porphyrize powder.Cooked Radix Notoginseng 3: get clean Radix Notoginseng, clean, profit 24h is to saturating earlier, and high pressure steams 45rain again, takes out, and cools section, drying, porphyrize powder.
Reports such as Ke Jinhu [" method that steams is concocted the influence of cooked Radix Notoginseng powder to saponin content ", " time precious traditional Chinese medical science traditional Chinese medicines research ", 2003,14 (8): 475], the preparation of cooked Radix Notoginseng powder is got the raw sangqi ginseng powder and is handled 3h at 100 ℃ of flowing steams, takes out and smashs to pieces after drying.
Above bibliographical information raw sangqi ginseng concoct and fried, the concocting method that steams of cooked Radix Notoginseng, its method differs, the result differs, and lacks quality, drug effect foundation.
Summary of the invention
Purpose of the present invention just provides a kind of concocting method of cooked Radix Notoginseng, and the cooked Radix Notoginseng period of storage that this method is made is long, steady quality, has enlarged the clinical practice of Radix Notoginseng, science, standard.
In order to realize goal of the invention, the technical solution used in the present invention comprises the steps:
The Radix Notoginseng of getting raw sangqi ginseng or running through; Or raw sangqi ginseng sheet, the Radix Notoginseng Tabellae that runs through; Or Radix Notoginseng powder; Put that 106～125 ℃ of high temperature, high pressure 0.03～0.14MPa steam 1～15 hour in the flowing steam pot, to inside and outside be sepia, take out, promptly.
Raw sangqi ginseng is the root and rhizome of panax araliaceae plant Panax notoginseng (Burk.) F.H.Chen, and gather back unhairing root, pruning are cleaned.
The Radix Notoginseng that runs through is the dry root and rhizome of panax araliaceae plant Panax notoginseng (Burk.) F.H.Chen, adds water infiltration or the infiltration of heating by Radix Notoginseng: water=1:0.5～3 part by weight, and is permeable most to medicine, cuts no white core behind the Radix Notoginseng open.
The raw sangqi ginseng sheet is the root and rhizome of panax araliaceae plant Panax notoginseng (Burk.) F.H.Chen, and section is cleaned in gather back unhairing root, pruning.
The Radix Notoginseng Tabellae that runs through requires 3 the Radix Notoginseng that runs through for the weighting profit, through section; Or get the drying sheet of the root and rhizome of panax araliaceae plant Panaxnotoginseng (Burk.) F.H.Chen, and add water infiltration or the infiltration of heating by Radix Notoginseng: water=1:0.2～2 part by weight, permeable most to medicine, cut no white core behind the Radix Notoginseng Tabellae open.
Radix Notoginseng powder is the dry root and rhizome of panax araliaceae plant Panax notoginseng (Burk.) F.H.Chen or the drying sheet of root and rhizome through being processed into of Radix Notoginseng, through being processed into powder [sieve (10 order)～No. nine sieves (200 order)].
Baking temperature is 40～150 ℃ or natural drying.
With the cooked Radix Notoginseng of concocting method gained of the present invention, its processing amount of cure is higher than and is better than the method that steams, the fried method of tradition, and product quality also is better than the cooked Radix Notoginseng of the method that steams, traditional frying method gained; Concocting method of the present invention also is different from the method that steams of bibliographical information, has clearly stipulated the degree of the temperature, pressure, time and the process of preparing Chinese medicine that steam, and relatively standardization, scientific has obvious application and promotional value.
Long, the steady quality of cooked Radix Notoginseng waiting time with concocting method gained of the present invention can be stablized and deposit more than 2 years.
Also proved composition and amount through HPLC finger printing test, had obviously differently, concocted into cooked Radix Notoginseng for Radix Notoginseng experimental basis is provided with raw sangqi ginseng with the cooked Radix Notoginseng of concocting method gained of the present invention.
Through pharmacology teaching and research room of Guangxi Medical University, the Pharmacodynamic test of active extract of carrying out with different animals, different pharmacological model proves, cooked Radix Notoginseng with concocting method gained of the present invention, tangible blood tonification effect is arranged, further verified " give birth to and drive ripe benefit away " theory of Chinese medical science of Radix Notoginseng, acute toxicity test proof cooked Radix Notoginseng toxicity is less, is suitable for clinical safety and uses.
Therefore, the present invention for the clinical practice of Radix Notoginseng enlarged by way of, for cooked Radix Notoginseng provides a kind of brand-new standardization, scientific concocting method.
Description of drawings
Fig. 1 is a Radix Notoginseng HPLC finger printing.
Fig. 2 is that 110 ℃, pressure 0.05MPa steam 2.5 hours cooked Radix Notoginseng HPLC finger printing.
The specific embodiment
The process of preparing Chinese medicine embodiment of cooked Radix Notoginseng
Embodiment 1
Get 120 Radix Notoginseng 5kg, add water 5kg, soaked into 48 hours down,, put in the flowing steam pot 115 ℃, pressure 0.07MPa and steam 6 hours to permeable to the greatest extent, the softening saturating heart of medicine at 30 ℃, to inside and outside be sepia, put coldly, take out, thinly slice, 100 ℃ of dryings, promptly.
Get Radix Notoginseng powder 5kg, put in the flowing steam pot 120 ℃, pressure 0.1MPa and steam 2 hours, to inside and outside be sepia, put coldly, take out, 90 ℃ of dryings, promptly.
Get the Radix Notoginseng Tabellae 5kg that runs through, put in the flowing steam pot 110 ℃, pressure 0.05MPa and steam 4 hours, to inside and outside be sepia, put coldly, take out, 80 ℃ of dryings, promptly.
The enrich blood pharmacodynamics and the toxicological experiment research data of cooked Radix Notoginseng
One, material
1, be subjected to test product cooked Radix Notoginseng, Yunnan raw sangqi ginseng, Guangxi raw sangqi ginseng etc., sample is 80 order powderies.Provide by GuangXi Medicine Institute.
2, animal Kunming mouse, regular grade, male and female are usefulness all, body weight 18-24 gram, Guangxi Medical University's medical experiment animal center provides.
3, other medicines cyclophosphamide, Hualian Pharmaceutical Co., Ltd., Shanghai, lot number 031014.
Two, method and result
1, to the influence of normal mouse peripheral blood erythrocyte, leukocyte, content of hemoglobin and coagulation function
Test uses mice, animal to be divided into blank group, the life of raw sangqi ginseng (Yunnan) group, Radix Notoginseng (Guangxi) group, cooked Radix Notoginseng group at random.Every mice vena ophthalmica of every treated animal is got blood, to its erythrocyte (RBC), leukocyte (WBC), count (blood counting chamber counting method), and surveys hemoglobin (HB) content (cyanmethemoglobin method).Every group begins administration then, and the blank group is irritated the stomach distilled water, and each administration group is irritated stomach respectively and given corresponding given the test agent, and raw sangqi ginseng (Yunnan) group is given birth to, Radix Notoginseng (Guangxi) is organized, the dosage of cooked Radix Notoginseng group is 3g/kg.Irritate the long-pending 20ml/kg that is of body of stomach.Successive administration 10 days.RBC, WBC, HB are observed in same blood sampling in the 11st day.
The cooked Radix Notoginseng group is to the RBC effect of improving of normal mouse as a result, sees Table 1, table 2, table 3.
The influence (mean ± standard deviation) of the RBC of table 1 pair normal mouse
Compare the P value all greater than 0.05 with value before the medicine
The influence (mean ± standard deviation) of the WBC of table 2 pair normal mouse
Compare the P value all greater than 0.05 with value before the medicine
The influence (mean ± standard deviation) of the HB of table 3 pair normal mouse
Compare the P value all greater than 0.05 with value before the medicine
2, to " losing blood property " anemia mice peripheral blood erythrocyte, leukocyte, content of hemoglobin and clotting time, the influence in bleeding time
Test uses mice, animal to be divided into model control group, the life of raw sangqi ginseng (Yunnan) group, Radix Notoginseng (Guangxi) group, cooked Radix Notoginseng group at random.Every mice vena ophthalmica of every treated animal is got blood, to its erythrocyte (RBC), leukocyte (WBC), count (blood counting chamber counting method), and surveys hemoglobin (HB) content (cyanmethemoglobin method).Every mice vena ophthalmica blood-letting 0.5ml.Surveyed RBC, HB, WBC on the 2nd day more equally, RBC, HB, WBC comparison with before the blood-letting judge whether to cause " losing blood property " anemia.The successful back of modeling begins administration for every group, and the blank group is irritated the stomach distilled water, and each administration group is irritated stomach respectively and given suitable given the test agent, and raw sangqi ginseng (Yunnan) group is given birth to, Radix Notoginseng (Guangxi) is organized, the dosage of cooked Radix Notoginseng group is 3g/kg.Irritate the long-pending 20ml/kg that is of body of stomach.Successive administration is same blood sampling observation RBC, WBC, HB after 10 days, observes clotting time of mice (glass capillary method) and bleeding time (tail vein method) simultaneously.
The result shows cooked Radix Notoginseng group " losing blood property " anemia mice peripheral blood erythrocyte that can obviously raise, and HB is also had certain rising effect, and each group is not seen influential to WBC, sees Table 4, table 5, table 6; The cooked Radix Notoginseng group can obviously prolong " losing blood property " anemia mice clotting time, sees Table 7.
Table 4 is to the influence (mean ± standard deviation) of " losing blood property " anemia mice RBC
With before the modeling relatively:
*P＜0.05,
*P＜0.01; Compare with model control group: △ P＜0.05, △ △ P＜0.01
Table 5 is to the influence (mean ± standard deviation) of " losing blood property " anemia mice WBC
With before the modeling relatively the P value all greater than 0.05; Each administration group WBC changing value and model control group compare the P value all greater than 0.05
Table 6 is to the influence (mean ± standard deviation) of " losing blood property " anemia mice HB
With before the modeling relatively:
*P＜0.05,
*P＜0.01; Compare with model control group: △ P＜0.05
Table 7 is to " losing blood property " anemia mice clotting time and the influence in bleeding time (mean ± standard deviation)
With before the modeling relatively: * P＜0.05,
*P＜0.01; Compare with model control group: △ P＜0.05, △ △ P＜0.01
3, cooked Radix Notoginseng is to the influence of " losing blood property " anemia mice peripheral blood erythrocyte, leukocyte, content of hemoglobin
Test uses mice, animal to be divided into model control group, cooked Radix Notoginseng group at random.Every mice vena ophthalmica of every treated animal is got blood, to its erythrocyte (RBC), leukocyte (WBC), count (blood counting chamber counting method), and surveys hemoglobin (HB) content (cyanmethemoglobin method).Every mice vena ophthalmica blood-letting 0.5ml.Surveyed RBC, HB, WBC on the 2nd day more equally, RBC, HB, WBC comparison with before the blood-letting judge whether to cause " losing blood property " anemia.Other gets 20 not blood-letting of mice as the normal control group.Modeling success back begins administration for every group, and the blank group is irritated the stomach distilled water, and the dosage of cooked Radix Notoginseng group is respectively 3g/kg, 1.5g/kg.Irritate the long-pending 20ml/kg that is of body of stomach.Successive administration is same blood sampling observation RBC, WBC, HB after 10 days.
The result shows that cooked Radix Notoginseng has tangible rising effect to " losing blood property " anemia mice peripheral blood erythrocyte, has tangible rising effect to " losing blood property " anemia mice periphery content of hemoglobin; Leukocyte is not had obvious influence, see Table 8, table 9, table 10.
Table 8 cooked Radix Notoginseng is to the influence (mean ± standard deviation) of " losing blood property " anemia mice RBC
With before the modeling relatively: * P＜0.05,
*P＜0.01; Compare with model control group: △ P＜0.05, △ △ P＜0.01
Table 9 cooked Radix Notoginseng is to the influence (mean ± standard deviation) of " losing blood property " anemia mice WBC
With before the modeling relatively the P value all greater than 0.05; Each administration group WBC changing value and model control group compare the P value all greater than 0.05
Table 10 cooked Radix Notoginseng is to the influence (mean ± standard deviation) of " losing blood property " anemia mice HB
With before the modeling relatively: * P＜0.05,
*P＜0.01; Compare with model control group: △ P＜0.05
4, cooked Radix Notoginseng is to the influence of cyclophosphamide " blood deficiency " mice peripheral blood erythrocyte, leukocyte, content of hemoglobin
Test uses mice, animal to be divided into model control group, cooked Radix Notoginseng group at random.Every group of mice got blood, to its erythrocyte (RBC), leukocyte (WBC), count (blood counting chamber counting method), and surveys hemoglobin (HB) content (cyanmethemoglobin method).Every group of mice be in the 1st day, the 2nd day intraperitoneal injection of cyclophosphamide 100mg/kg, the 3rd day, the 4th day intraperitoneal injection of cyclophosphamide 120mg/kg.Other gets 20 of mices and does not inject cyclophosphamide as the normal control group.Each group beginning administration in the 5th day, the blank group is irritated the stomach distilled water, and the dosage of cooked Radix Notoginseng group is 3g/kg, 1.5g/kg, irritates the long-pending 20ml/kg that is of body of stomach.Successive administration is same blood sampling observation RBC, WBC, HB after 10 days.
The result shows that peripheral blood erythrocyte, content of hemoglobin that cooked Radix Notoginseng causes cyclophosphamide all have tangible rising effect, but peripheral leukocytes is not seen influential.See Table 11, table 12, table 13.
Table 11 cooked Radix Notoginseng is to the influence (mean ± standard deviation) of cyclophosphamide " blood deficiency " mice RBC
Annotate: (1) is relatively preceding with modeling:
*P＜0.05,
*P＜0.01; Compare with model control group: △ P＜0.05, △ △ P＜0.01
(2) to protective rate (%)=(administration group RBC value deducts model group RBC value)/model group RBC value of RBC
Table 12 cooked Radix Notoginseng is to the influence (mean ± standard deviation) of cyclophosphamide " blood deficiency " mice WBC
Annotate: (1) is relatively preceding with modeling:
*P＜0.05,
*P＜0.01; Compare with model control group: △ P＜0.05, △ △ P＜0.01
(2) to rate of rise (%)=(administration group WBC value deducts model group WBC value)/model group WBC value of WBC
Table 13 cooked Radix Notoginseng is to the influence (mean ± standard deviation) of cyclophosphamide " blood deficiency " mice HB
Annotate: (1) is relatively preceding with modeling:
*P＜0.05,
*P＜0.01; Compare with model control group: △ P＜0.05, △ △ P＜0.01
(2) to protective rate (%)=(administration group HB value deducts model group HB value)/model group HB value of HB
5, cooked Radix Notoginseng is to the influence of cyclophosphamide " blood deficiency " mouse bone marrow cells nucleated cell and spleen weight
Test uses mice, animal to be divided into model control group, cooked Radix Notoginseng group at random.Every group of mice be in the 1st day, the 2nd day intraperitoneal injection of cyclophosphamide 100mg/kg, the 3rd day,, the 4th day intraperitoneal injection of cyclophosphamide 120mg/kg.Other gets 20 of mices and does not inject cyclophosphamide as the normal control group.Each group beginning administration in the 5th day, the blank group is irritated the stomach distilled water, and the dosage of cooked Radix Notoginseng group is 3g/kg, 1.5g/kg, irritates the long-pending 20ml/kg that is of body of stomach.Successive administration is got mouse bone marrow cells counting bone marrow nucleated cell after 10 days, get spleen and weigh.
Test shows, mice gives the quick hypertrophy of bone marrow nucleated cell behind the cyclophosphamide continuously, and " compensatory " occurring increases, and the cooked Radix Notoginseng group has inhibitory action to " compensatory " phenomenon of this cyclophosphamide, the results are shown in Table 14.The rising effect of inferring peripheral blood erythrocyte that cooked Radix Notoginseng causes cyclophosphamide, content of hemoglobin is relevant with promotion procedure for peripheral blood ability.
Spleen weight reduced after mice gave cyclophosphamide continuously; show that cyclophosphamide makes mice procedure for peripheral blood ability drop; the spleen weight that cooked Radix Notoginseng causes cyclophosphamide descends the certain protection effect is all arranged, and relatively has evident difference with model control group, sees Table 14.
Table 14 cooked Radix Notoginseng is to the influence (mean ± standard deviation) of cyclophosphamide " blood deficiency " mouse bone marrow cells nucleated cell and spleen stolen goods weight
Annotate: compare with the normal control group (1):
*P＜0.05,
*P＜0.01; Compare with model control group: △ P＜0.05, △ △ P＜0.01
(2) to protective rate (%)=(administration group spleen weight deducts the model group the spleen weight)/model group spleen weight of spleen
6, the acute toxicity test of cooked Radix Notoginseng
Take by weighing 4 parts of each 20g of cooked Radix Notoginseng sample, adding distil water is to 70ml, and what be mixed with that every ml contains medicated powder 0.286g is subjected to reagent liquid.
Get 80 of mices, be divided into 4 groups at random, 20 every group, every group of male and female half and half irritate respectively that stomach gives B, C, E, F are subjected to reagent liquid, each every 0.8ml, and every administration in 8 hours 1 time, administration is 3 times altogether in 24 hours.Reach the reaction of observing mice in 7 days during the administration, and have or not death and other exception table now.The result shows that it is normal that cooked Radix Notoginseng is respectively organized the mice mental status, and defecation and hair no abnormality seen are not seen death condition yet.Each is organized the mice body weight and sees that all increase is arranged, and does not see the decline that causes because of the medicine factor, sees Table 15.The maximum tolerated dose that calculates cooked Radix Notoginseng is 34.32g/kg.
The acute toxicity test of table 15 cooked Radix Notoginseng is to the influence (mean ± standard deviation) of mice body weight
Above pharmacodynamic experiment shows that cooked Radix Notoginseng has the pharmacological action of enriching blood, and its toxicity is lower.
The HPLC determining fingerprint pattern of cooked Radix Notoginseng
Chromatographic condition and system suitability test: with the octadecylsilane chemically bonded silica is filler, is mobile phase A with the acetonitrile, is Mobile phase B with water, and the regulation in the according to the form below is carried out gradient elution; The detection wavelength is 203nm.Number of theoretical plate calculates by the arasaponin R1 peak should be not less than 8000.
| Time (min) water (%) acetonitrile (%) |
| 0 81 19 12 81 19 70 64 36 80 64 36 |
The preparation of reference substance solution: it is an amount of that precision takes by weighing ginsenoside Rg l reference substance, ginsenoside Rbl reference substance and arasaponin R1 reference substance, add methanol and make the mixed solution that every 1ml contains ginsenoside Rg l 0.4mg, ginsenoside Rbl 0.4mg, arasaponin R1 0.1mg, promptly.
The preparation of need testing solution: get the about 0.6g of this product powder (crossing sieve No. four), the accurate title, decide, and the accurate methanol 25ml that adds claims to decide weight, put in 80 ℃ of water-baths and to reflux 2 hours, put coldly, claim again to decide weight, supply the weight that subtracts mistake with methanol, shake up, filter, get subsequent filtrate, promptly.
Algoscopy: measure according to high performance liquid chromatography Chinese Pharmacopoeia version in 2005 one (appendix VID), accurate respectively reference substance solution and each 20 μ l of need testing solution of drawing inject chromatograph of liquid, measure, promptly.
Radix Notoginseng, 110 ℃ and pressure 0.05MPa are steamed 2.5 hours cooked Radix Notoginseng, measure by above HPLC method, the finger printing of gained is seen Fig. 1, Fig. 2.
As seen, the cooked Radix Notoginseng finger printing than the Radix Notoginseng finger printing many 9,10, No. 11 peaks, 1, the peak area at 2,3, No. 7 peaks also decreases.After illustrating that Radix Notoginseng becomes cooked Radix Notoginseng after high pressure, high temperature are concocted, qualitative change and quantitative change have all taken place in its chemical analysis kind and quantity.
Claims (7)
1, a kind of concocting method of cooked Radix Notoginseng is characterized in that comprising the steps: the Radix Notoginseng of getting raw sangqi ginseng or running through; Or raw sangqi ginseng sheet, the Radix Notoginseng Tabellae that runs through; Or Radix Notoginseng powder; Put in the flowing steam pot 106～125 ℃, 0.03～0.14MPa and steam 1～15 hour, to inside and outside be sepia, take out drying.
2, the concocting method of a kind of cooked Radix Notoginseng as claimed in claim 1 is characterized in that: raw sangqi ginseng is the root and rhizome of panax araliaceae plant Panax notoginseng (Burk.) F.H.Chen, and gather back unhairing root, pruning are cleaned.
3, the concocting method of a kind of cooked Radix Notoginseng as claimed in claim 1, it is characterized in that: the Radix Notoginseng that runs through is the dry root and rhizome of panax araliaceae plant Panax notoginseng (Burk.) F.H.Chen, add water infiltration or heat infiltration by Radix Notoginseng: water=1:0.5～3 part by weight, permeable most to medicine, cut no white core behind the Radix Notoginseng open.
4, the concocting method of a kind of cooked Radix Notoginseng as claimed in claim 1 is characterized in that: the raw sangqi ginseng sheet is the root and rhizome of panax araliaceae plant Panax notoginseng (Burk.) F.H.Chen, and section is cleaned in gather back unhairing root, pruning.
5, the concocting method of a kind of cooked Radix Notoginseng as claimed in claim 1 is characterized in that: the Radix Notoginseng Tabellae that runs through is the Radix Notoginseng that runs through of claim 3, through section; Or get the drying sheet of the root and rhizome of panax araliaceae plant Panax notoginseng (Burk.) F.H.Chen, and add water infiltration or the infiltration of heating by Radix Notoginseng: water=1:0.2～2 weight ratios, permeable most to medicine, cut no white core behind the Radix Notoginseng Tabellae open.
6, the concocting method of a kind of cooked Radix Notoginseng as claimed in claim 1, it is characterized in that: Radix Notoginseng powder is the dry root and rhizome of panax araliaceae plant Panax notoginseng (Burk.) F.H.Chen or the drying sheet of root and rhizome through being processed into of Radix Notoginseng, through being processed into powder.
7, the concocting method of a kind of cooked Radix Notoginseng as claimed in claim 1 is characterized in that: baking temperature is 40～150 ℃ or natural drying.
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| CNA2009101139148A CN101502548A (en) | 2009-03-13 | 2009-03-13 | Method for processing cooked notoginseng |
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| CNA2009101139148A CN101502548A (en) | 2009-03-13 | 2009-03-13 | Method for processing cooked notoginseng |
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| US20130324486A1 (en) * | 2011-03-08 | 2013-12-05 | Hong Sik Lee | Method for producing cultured root of purple ginseng |
| CN104800257A (en) * | 2014-01-24 | 2015-07-29 | 成都雪瑞安健康管理有限公司 | Vinegar processed pseudo-ginseng and processing technology and application thereof |
| CN106138141A (en) * | 2016-08-08 | 2016-11-23 | 四川仟源中药饮片有限公司 | A kind of cooked Radix Notoginseng and preparation method thereof |
| CN106491678A (en) * | 2016-10-11 | 2017-03-15 | 文山学院 | A kind of processing method of cooked Radix Notoginseng and purposes |
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- 2009-03-13 CN CNA2009101139148A patent/CN101502548A/en active Pending
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| US20130324486A1 (en) * | 2011-03-08 | 2013-12-05 | Hong Sik Lee | Method for producing cultured root of purple ginseng |
| US9585904B2 (en) * | 2011-03-08 | 2017-03-07 | Hong Sik Lee | Method for producing cultured root of purple ginseng |
| CN104800257A (en) * | 2014-01-24 | 2015-07-29 | 成都雪瑞安健康管理有限公司 | Vinegar processed pseudo-ginseng and processing technology and application thereof |
| CN104800257B (en) * | 2014-01-24 | 2018-04-27 | 成都雪瑞安健康管理有限公司 | A kind of ripe pseudo-ginseng of vinegar system and its processing procedure and application |
| CN106138141A (en) * | 2016-08-08 | 2016-11-23 | 四川仟源中药饮片有限公司 | A kind of cooked Radix Notoginseng and preparation method thereof |
| CN106138141B (en) * | 2016-08-08 | 2019-09-13 | 四川仟源中药饮片有限公司 | A kind of ripe Radix Notoginseng and preparation method thereof |
| CN106491678A (en) * | 2016-10-11 | 2017-03-15 | 文山学院 | A kind of processing method of cooked Radix Notoginseng and purposes |
| CN107308196A (en) * | 2017-07-11 | 2017-11-03 | 天马（安徽）国药科技股份有限公司 | A kind of concocting method of pseudo-ginseng |
| CN108926587A (en) * | 2018-08-22 | 2018-12-04 | 云南中医学院 | A kind of boiling hot concocting method of the sand of ripe Radix Notoginseng |
| CN115645445A (en) * | 2022-10-19 | 2023-01-31 | 北京中医药大学 | A processing method of novel cooked Notoginseng radix for preventing and treating Alzheimer disease and related products thereof |
| CN115645445B (en) * | 2022-10-19 | 2023-12-19 | 北京中医药大学 | Processing technology of novel cooked pseudo-ginseng capable of preventing and treating Alzheimer disease and related products thereof |
| CN116687989A (en) * | 2023-07-07 | 2023-09-05 | 山东中医药大学 | Preparation method of pseudo-ginseng decoction pieces |
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Open date: 20090812 |