CN110151805A - A kind of ripe Radix Notoginseng and preparation method thereof and antioxidation application - Google Patents
A kind of ripe Radix Notoginseng and preparation method thereof and antioxidation application Download PDFInfo
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- ginsenoside
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- 241000180649 Panax notoginseng Species 0.000 title claims abstract description 101
- 235000003143 Panax notoginseng Nutrition 0.000 title claims abstract description 101
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 230000003064 anti-oxidating effect Effects 0.000 title claims abstract description 13
- 229930182494 ginsenoside Natural products 0.000 claims abstract description 86
- 229940089161 ginsenoside Drugs 0.000 claims abstract description 86
- 239000001397 quillaja saponaria molina bark Substances 0.000 claims abstract description 32
- 229930182490 saponin Natural products 0.000 claims abstract description 32
- 150000007949 saponins Chemical class 0.000 claims abstract description 32
- OZTXYFOXQFKYRP-TXRYYSRHSA-N Ginsenoside Rh4 Chemical compound O([C@@H]1[C@H]2C(C)(C)[C@@H](O)CC[C@]2(C)[C@H]2C[C@@H](O)[C@H]3[C@@]([C@@]2(C1)C)(C)CC[C@@H]3C(/C)=C/CC=C(C)C)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O OZTXYFOXQFKYRP-TXRYYSRHSA-N 0.000 claims abstract description 14
- AVXFIVJSCUOFNT-QXPABTKOSA-N (2R,3R,4S,5S,6R)-2-[[(3S,5R,6S,8R,9R,10R,12R,13R,14R,17S)-3,12-dihydroxy-4,4,8,10,14-pentamethyl-17-(6-methylhepta-1,5-dien-2-yl)-2,3,5,6,7,9,11,12,13,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-6-yl]oxy]-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O([C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1)[C@@H]1[C@H]2C(C)(C)[C@@H](O)CC[C@]2(C)[C@@H]2[C@](C)([C@@]3(C)[C@H]([C@H](O)C2)[C@@H](C(=C)CC/C=C(\C)/C)CC3)C1 AVXFIVJSCUOFNT-QXPABTKOSA-N 0.000 claims abstract description 13
- YURJSTAIMNSZAE-HHNZYBFYSA-N ginsenoside Rg1 Chemical compound O([C@@](C)(CCC=C(C)C)[C@@H]1[C@@H]2[C@@]([C@@]3(C[C@@H]([C@H]4C(C)(C)[C@@H](O)CC[C@]4(C)[C@H]3C[C@H]2O)O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)C)(C)CC1)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O YURJSTAIMNSZAE-HHNZYBFYSA-N 0.000 claims abstract description 10
- GZYPWOGIYAIIPV-JBDTYSNRSA-N ginsenoside Rb1 Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O)O[C@@](C)(CCC=C(C)C)[C@@H]1[C@@H]2[C@@]([C@@]3(CC[C@H]4C(C)(C)[C@@H](O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O)CC[C@]4(C)[C@H]3C[C@H]2O)C)(C)CC1)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O GZYPWOGIYAIIPV-JBDTYSNRSA-N 0.000 claims abstract description 8
- RAQNTCRNSXYLAH-RFCGZQMISA-N (20S)-ginsenoside Rh1 Chemical compound O([C@@H]1[C@H]2C(C)(C)[C@@H](O)CC[C@]2(C)[C@H]2C[C@@H](O)[C@H]3[C@@]([C@@]2(C1)C)(C)CC[C@@H]3[C@@](C)(O)CCC=C(C)C)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O RAQNTCRNSXYLAH-RFCGZQMISA-N 0.000 claims abstract description 7
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims description 23
- 235000003140 Panax quinquefolius Nutrition 0.000 claims description 23
- 235000008434 ginseng Nutrition 0.000 claims description 23
- 238000000034 method Methods 0.000 claims description 17
- 239000000843 powder Substances 0.000 claims description 14
- 238000007873 sieving Methods 0.000 claims description 10
- 244000131316 Panax pseudoginseng Species 0.000 claims description 9
- 239000003814 drug Substances 0.000 claims description 5
- 229930182470 glycoside Natural products 0.000 claims description 4
- 150000002338 glycosides Chemical class 0.000 claims description 4
- 239000000344 soap Substances 0.000 claims description 4
- 235000003181 Panax pseudoginseng Nutrition 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000010025 steaming Methods 0.000 claims description 3
- 229940079593 drug Drugs 0.000 claims description 2
- 230000001590 oxidative effect Effects 0.000 claims 1
- 230000003078 antioxidant effect Effects 0.000 abstract description 10
- 241000208340 Araliaceae Species 0.000 description 18
- -1 DPPH radicals Chemical class 0.000 description 18
- 239000000284 extract Substances 0.000 description 9
- 239000004615 ingredient Substances 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- 230000002000 scavenging effect Effects 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 238000001514 detection method Methods 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 238000003908 quality control method Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 238000002835 absorbance Methods 0.000 description 4
- 230000007760 free radical scavenging Effects 0.000 description 4
- 239000013558 reference substance Substances 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 3
- 239000012085 test solution Substances 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- DGEZNRSVGBDHLK-UHFFFAOYSA-N [1,10]phenanthroline Chemical compound C1=CN=C2C3=NC=CC=C3C=CC2=C1 DGEZNRSVGBDHLK-UHFFFAOYSA-N 0.000 description 2
- 239000006286 aqueous extract Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 229960000935 dehydrated alcohol Drugs 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000010992 reflux Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 235000021411 American diet Nutrition 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 229910021577 Iron(II) chloride Inorganic materials 0.000 description 1
- WEVYAHXRMPXWCK-UHFFFAOYSA-N acetonitrile Substances CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000036592 analgesia Effects 0.000 description 1
- 230000003026 anti-oxygenic effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000012631 diagnostic technique Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- TUJKJAMUKRIRHC-UHFFFAOYSA-N hydroxyl Chemical compound [OH] TUJKJAMUKRIRHC-UHFFFAOYSA-N 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 238000012417 linear regression Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004089 microcirculation Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7028—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages
- A61K31/7034—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin
- A61K31/704—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin attached to a condensed carbocyclic ring system, e.g. sennosides, thiocolchicosides, escin, daunorubicin
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
A kind of ripe Radix Notoginseng, by weight percentage, the Panax Notoginseng saponin R contained in the ripe Radix Notoginseng1, ginsenoside Rg1, ginsenoside Rb1The total amount of three is 0.35-5.48%, the ginsenoside Rh contained in the ripe Radix Notoginseng1, ginsenoside Rk3, ginsenoside Rh4, ginsenoside 20 (S)-Rg3With ginsenoside 20 (R)-Rg3Five total amount is 0.26-6.30%；The Panax Notoginseng saponin R contained in the ripe Radix Notoginseng1, ginsenoside Rg1, ginsenoside Rb1, ginsenoside Rh1, ginsenoside Rk3, ginsenoside Rh4, ginsenoside 20 (S)-Rg3With ginsenoside 20 (R)-Rg3Eight total amount is 5.61-6.66%.Also disclose the preparation method and antioxidation application of ripe Radix Notoginseng.The ripe Radix Notoginseng of the application has preferable antioxidant activity.
Description
Technical field
This application involves but be not limited to pharmaceutical technology field, be particularly, but not limited to a kind of ripe Radix Notoginseng and preparation method thereof
And purposes.
Background technique
Radix Notoginseng is araliaceae ginseng plant, is famous medicinal plant, more than 400 years existing for treating cardiovascular disease
History.According to nineteen forty-four version " American diet replenishers health and Education Act ", Radix Notoginseng and its Related product are also listed in diet
Replenishers.Traditionally, Radix Notoginseng has raw, ripe two kinds of forms, and raw sangqi ginseng is used for hemostasis, analgesia and activating microcirculation and removing stasis medicinal, and ripe Radix Notoginseng is then
It is help salubrity, the invigorant for improving health.Studies have shown that raw sangqi ginseng, after steaming, pharmacological action is changed.So
And up to the present, country variant pharmacopeia has only recorded raw sangqi ginseng medicinal material and its medicine materical crude slice Radix Notoginseng powder, does not include other processed products
Kind.This illustrates that the objective difference of raw, ripe Radix Notoginseng is not recognized by national code also.
Although multinomial research it has been reported that the chemical component of Radix Notoginseng had occurred in concocting process conspicuousness variation, one
It is related in the professional standard of ripe Radix Notoginseng a bit, it is still consistent with raw sangqi ginseng to the marked member of ripe Radix Notoginseng quality control.For example, in state
In food and medicine Surveillance Authority, the family national drug standards, the quality control marks ingredient to ripe Radix Notoginseng powder is ginsenoside Rb1、
Ginsenoside Rg1And Panax Notoginseng saponin R1, this is identical as the quality control index of raw sangqi ginseng in 2015 editions Chinese Pharmacopoeias.It is distinguished due to lacking
The quality control standard of raw, ripe Radix Notoginseng, lead to people there are curative effects during using life, ripe Radix Notoginseng it is unstable, even cause not
The risk of good reaction.
Therefore, develop a kind of stable and ripe Radix Notoginseng product with desired activity index ingredient of curative effect and its preparation and
Detection method carries out individuation control to life, ripe Radix Notoginseng and is of great significance.
Summary of the invention
It is the general introduction to the theme being described in detail herein below.This general introduction is not the protection model in order to limit claim
It encloses.
Ripe Radix Notoginseng product and its system stable and with desired activity index ingredient this application provides a kind of curative effect
Preparation Method and antioxidation application.
Specifically, this application provides a kind of ripe Radix Notoginseng, by weight percentage, the Radix Notoginseng soap contained in the ripe Radix Notoginseng
Glycosides R1, ginsenoside Rg1With ginsenoside Rb1The total amount of three is 0.35-5.48%, wherein Panax Notoginseng saponin R1Content be 0-
1.17%, ginsenoside Rg1Content be 0-2.70%, ginsenoside Rb1Content be 0.35-2.10%；In the ripe Radix Notoginseng
The ginsenoside Rh contained1, ginsenoside Rk3, ginsenoside Rh4, ginsenoside 20 (S)-Rg3With ginsenoside 20 (R)-Rg3
Five total amount is 0.26-6.30%, wherein ginsenoside Rh1Content be 0.06-0.46%, ginsenoside Rk3Content
For 0.04-1.71%, ginsenoside Rh4Content be 0.17-2.91%, ginsenoside 20 (S)-Rg3Content be 0-
0.88%, ginsenoside 20 (R)-Rg3Content be 0-0.55%；The Panax Notoginseng saponin R contained in the ripe Radix Notoginseng1, ginsenoside
Rg1, ginsenoside Rb1, ginsenoside Rh1, ginsenoside Rk3, ginsenoside Rh4, ginsenoside 20 (S)-Rg3And ginsenoside
20(R)-Rg3Eight total amount is 5.61-6.66%.
In some embodiments, by weight percentage, the Panax Notoginseng saponin R contained in the ripe Radix Notoginseng1, ginsenoside
Rg1With ginsenoside Rb1The total amount of three can be 0.35-2.59%, wherein Panax Notoginseng saponin R1Content can be 0-
0.24%, ginsenoside Rg1Content can be 0-1.18%, ginsenoside Rb1Content can be 0.35-1.17%；It is described
The ginsenoside Rh contained in ripe Radix Notoginseng1, ginsenoside Rk3, ginsenoside Rh4, ginsenoside 20 (S)-Rg3And ginsenoside
20(R)-Rg3Five total amount can be 3.02-6.30%, wherein ginsenoside Rh1Content can for 0.26-0.46%,
Ginsenoside Rk3Content can be 0.89-1.71%, ginsenoside Rh4Content can be 1.30-2.91%, ginsenoside
20(S)-Rg3Content can be 0.37-0.88%, ginsenoside 20 (R)-Rg3Content can be 0.20-0.55%；It is described
The Panax Notoginseng saponin R contained in ripe Radix Notoginseng1, ginsenoside Rg1, ginsenoside Rb1, ginsenoside Rh1, ginsenoside Rk3, ginseng soap
Glycosides Rh4, ginsenoside 20 (S)-Rg3With ginsenoside 20 (R)-Rg3Eight total amount is 5.61-6.66%.
In some embodiments, by weight percentage, the Panax Notoginseng saponin R contained in the ripe Radix Notoginseng1, ginsenoside
Rg1With ginsenoside Rb1The total amount of three can be 2.59%, wherein Panax Notoginseng saponin R1Content can be 0.24%, ginseng
Saponin(e Rg1Content can be 1.18%, ginsenoside Rb1Content can be 1.17%；The ginseng contained in the ripe Radix Notoginseng
Saponin(e Rh1, ginsenoside Rk3, ginsenoside Rh4, ginsenoside 20 (S)-Rg3With ginsenoside 20 (R)-Rg3Five total amount
It can be 3.02%, wherein ginsenoside Rh1Content can be 0.26%, ginsenoside Rk3Content can for 0.89%,
Ginsenoside Rh4Content can be 1.30%, ginsenoside 20 (S)-Rg3Content can be 0.37%, ginsenoside 20
(R)-Rg3Content can be 0.20%.The Panax Notoginseng saponin R contained in the ripe Radix Notoginseng1, ginsenoside Rg1, ginsenoside
Rb1, ginsenoside Rh1, ginsenoside Rk3, ginsenoside Rh4, ginsenoside 20 (S)-Rg3With ginsenoside 20 (R)-Rg3Eight
The total amount of person is 5.61%.
Present invention also provides a kind of methods for preparing ripe Radix Notoginseng as described above, and the method includes the following steps:
Pseudo-ginseng is taken, cleans, dries, is crushed, it is primary to be sieved, obtain Radix Notoginseng powder；
The Radix Notoginseng powder is steamed 2-10 hours at 105-120 DEG C；
It dry, pulverize, secondary sieving, obtain the ripe Radix Notoginseng.
In some embodiments, the temperature for steaming the Radix Notoginseng powder can be 120 DEG C, and the time can be 2 hours.
In some embodiments, the primary sieving can be 40-60 mesh, and the secondary sieving can be
40-60 mesh.
Optionally, the primary sieving can be 40 meshes, and the secondary sieving can be 40 meshes.
In some embodiments, the drying can be dry to constant weight at 45 DEG C.
Present invention also provides the antioxidation applications of ripe Radix Notoginseng as described above.
In some embodiments, the powder of the ripe Radix Notoginseng can be taken directly to reach the antioxidation application.
In some embodiments, the antioxidation application can prepare the use in anti-oxidation medicine for the ripe Radix Notoginseng
On the way.
The application specification preparation method of the ripe Radix Notoginseng with preferable antioxidation is Radix Notoginseng " the different different mark of effect " individual
Change the perfect of quality control standard to lay a good foundation, while meeting States Pharmacopoeia specifications component content, also takes into account processing after-ripening Radix Notoginseng
Newly-increased ingredient, You Hen Johnson & Johnson manage active rare saponin component-ginsenoside Rh1, ginsenoside Rk3, ginsenoside
Rh4, ginsenoside 20 (S)-Rg3With ginsenoside 20 (R)-Rg3.Had using the ripe Radix Notoginseng that the present processes are prepared
Preferable antioxidant activity, the ripe effect of life for having fully demonstrated Radix Notoginseng processed product is different, respectively there is the characteristics of its function.
Other features and advantage will illustrate in the following description, also, partly become from specification
It obtains it is clear that being understood and implementing the application.The purpose of the application and other advantages can be by specifications, right
Specifically noted structure is achieved and obtained in claim and attached drawing.
Detailed description of the invention
Attached drawing is used to provide to further understand technical scheme, and constitutes part of specification, with this
The embodiment of application is used to explain the technical solution of the application together, does not constitute the limitation to technical scheme.
Fig. 1 is the antioxidant activity figure of the ripe Radix Notoginseng of three batches of raw sangqi ginsengs and the preparation of embodiment 1.
Specific embodiment
For the purposes, technical schemes and advantages of the application are more clearly understood, below in conjunction with attached drawing to the application
Embodiment be described in detail.It should be noted that in the absence of conflict, in the embodiment and embodiment in the application
Feature can mutual any combination.
Used instrument includes: that (FW-135 type, upper oceanic rise open up the limited public affairs of instrument and equipment to pulverizer in following embodiment
Department)；Electronic analytical balance (CP114 type, Shanghai Ao Haosi Instrument Ltd.)；Ultrasonic washing instrument (BL10-250A type, on
Hai Bilang Instrument Ltd.)；Rotary Evaporators (RE-5205 type, Shanghai Yarong Biochemical Instrument Plant)；Electric-heated thermostatic water bath
(DZKW type, Beijing forever bright Medical Instruments Co., Ltd)；Pressure steam sterilization boiler (YXQ-LS-100SII-01-00, Shanghai
Medical Equipment Plant, Bo Xun Industrial Co., Ltd.)；(SPD-20A detector, Japanese Shimadzu are public for LC-20AB high performance liquid chromatograph
Department)；Superpure water machine (the excellent general series of UPT-1-20T, Chengdu Ultra Pure Science & Technology Co., Ltd)；Table-type high-speed refrigerated centrifuge
(H1650R type, Hunan Xiang Yi Laboratory Instruments development corporation, Ltd.)；Coagulo meter (XN06 series, Wuhan Jing Chuan diagnostic techniques stock
Part Co., Ltd)；Ultraviolet specrophotometer (Shimadzu 2600).
Embodiment 1
A method of ripe Radix Notoginseng being prepared, the method includes the following steps:
Three batch pseudo-ginseng main root samples for picking up from Yunnan mountain of papers are taken, cleans, dries, are crushed, 40 meshes is crossed, obtains
Radix Notoginseng powder；
Take the wherein a collection of Radix Notoginseng powder prepared respectively in high-pressure sterilizing pot, at different temperatures (105 DEG C, 110 DEG C,
120 DEG C), steam different time (2h, 4h, 6h, 8h, 10h)；
Taking-up is placed in electric drying oven with forced convection and dries to constant weight, smashs to pieces, crosses 40 meshes, obtains the ripe Radix Notoginseng.
Radix Notoginseng ingredient and its content detection
The ingredient and its content of the ripe Radix Notoginseng S4-S18 of raw sangqi ginseng S1-S3 and the preparation of embodiment 1 are detected using following methods:
(1) chromatographic condition is determined:
Chromatographic column: Vision HT C18Column (250mm × 4.6mm)；
Mobile phase: with water (A)-acetonitrile (B) for mobile phase；
Flow rate of mobile phase: 1.0mL/min；
Linear gradient elution condition: 0~20min, 20 volume %B；20~45min, 20 volume of volume %~46 %B；45
~55min:46 volume the volume of %~55 %B；55~60min:55 volume %B；
Detection wavelength: 203nm；
Column temperature: 24 DEG C；
Sample volume: 10 μ l；
Theoretical cam curve is greater than 4000；
(2) mixed reference substance solution is prepared:
Precision weighs Panax Notoginseng saponin R respectively1, ginsenoside Rg1、Rb1、Rh1、Rk3、Rh4、20(S)-Rg3With 20 (R)-Rg3
Reference substance 1.80,3.20,3.30,1.625,1.875,1.75,1.25,1.375mg are placed in 2mL measuring bottle, and it is fixed to be dissolved with methanol
Hold, being configured to mass concentration is respectively 0.90,1.60,1.65,0.8125,0.9375,0.875,0.625,0.6875mg/mL
Mixed reference substance solution；
(3) each 5g of ripe Radix Notoginseng powder that preparation test solution: weighing raw sangqi ginseng powder respectively and embodiment 1 is prepared, often
Secondary addition 50mL water refluxing extraction 2 times, each 2h, combined extract is concentrated into 50mL, and respectively obtaining concentration is 0.1g/ml's
Aqueous extracts；It shakes up, filters, take subsequent filtrate to be filtered with 0.45 μm of miillpore filter, obtain test solution；
(4) content of ingredient in test sample is measured:
The mixed reference substance solution and the test solution are detected using the chromatographic condition in step (1),
Calculate the content of the ingredient in the test sample according to the following equation according to the peak area measured；
Y=2705.6X+50.697, R2=0.9996 (1)
Y=3944.9X+69.409, R2=0.9997 (2)
Y=3227.3X+49.436, R2=0.9998 (3)
Y=9165X+63.297, R2=0.9999 (4)
Y=4948.7X+45.512, R2=0.9998 (5)
Y=4911.8X+33.761, R2=0.9998 (6)
Y=2949.24X+3.6774, R2=0.9998 (7)
Y=2172.6X+9.845, R2=0.9998 (8)
Formula (1)-(8) respectively represent Panax Notoginseng saponin R1, ginsenoside Rg1、Rb1、Rh1、Rk3、Rh4、20(S)-Rg3With 20
(R)-Rg3The equation of linear regression of 8 kinds of ingredients, X represent the concentration (mg/mL) of contained saponin(e, and Y represents peak area.
Testing result is see table 1.
Note: " -- " in table 1, which represents, to be not detected.
Component content in ripe Radix Notoginseng prepared by 1 raw sangqi ginseng of table and embodiment 1
The detection of Radix Notoginseng antioxidant activity
The antioxidant activity of the ripe Radix Notoginseng S4-S18 of raw sangqi ginseng S1-S3 and the preparation of embodiment 1 is detected using following methods:
(1) Radix Notoginseng 5g to be detected is weighed, is added 50mL water refluxing extraction 2 times, each 2h every time, combined extract, concentration
To 50mL, the Aqueous extracts that concentration is 0.1g/ml are respectively obtained；
(2) DPPH radicals scavenging power detects:
The extract for the Radix Notoginseng that step (1) is obtained is spare respectively with normal saline dilution to different concentration gradients；
It takes 1mL in test tube respectively the Notogineng Extract solution to be detected of various concentration, then is separately added into 3mL concentration and is
0.1mmol·L-1DPPH, every group 3 are parallel, are denoted as sample sets；
Three test tubes are taken, addition 3mL concentration is 0.1mmolL-1DPPH, then be separately added into 1mL dehydrated alcohol, be denoted as
Control group；
Each 1mL of Notogineng Extract solution to be detected for taking various concentration, adds 3mL dehydrated alcohol respectively, is denoted as blank
Group；
Sample sets, control group and blank group are both placed in dark place and stand 30min, measures the light absorption value at 517nm, point
A is not denoted as it1、A2、A3；
DPPH free radical scavenging activity is calculated according to the following equation:
DPPH free radical scavenging activity (%)=[1- (A1-A3)/A2] × 100%；
(3) hydroxyl radical free radical removes power detection:
It is spare by Notogineng Extract to be detected respectively with normal saline dilution to different concentration gradients；
It is 1.0mmolL that 60 μ L concentration are sequentially added into test tube-1FeCl2, 90 μ L concentration be 1.0mmolL-1's
1,10- phenanthroline, 2.4mL concentration are 0.2molL-1Phosphate buffer solution (PH 7.8), 150 μ L concentration be
0.17mol·L-1H2O2And the Notogineng Extract to be detected of 1.5mL various concentration, it is incubated at room temperature after 5min and is surveyed at 560nm
Determine absorbance；
Hydroxyl radical free radical clearance rate is calculated according to the following equation:
Hydroxyl radical free radical clearance rate (%)=[1- (A1-A2)/A0] × 100%
Wherein, A0For the absorbance of blank control group, no extract；A1For the absorbance of Radix Notoginseng sample to be detected；A2Not have
There is the absorbance of 1,10- phenanthroline.
The DPPH free radical scavenging activity and hydroxyl radical free radical obtained using the Notogineng Extract to be detected of various concentration is removed
Rate calculates EC by Graphpad Prism software50(concentration for reaching 50% maximum free radical scavenging activity), calculated result
See Fig. 1.In Fig. 1, A indicates that DPPH radicals scavenging force data, B indicate that hydroxyl radical free radical removes force data, number S1-S18
Meaning it is identical as table 1.Wherein, EC50Value is lower, indicates that antioxidant activity is stronger.
It will be seen from figure 1 that the DPPH radicals scavenging power EC of ripe Radix Notoginseng S5-S1850Value is below raw sangqi ginseng S1-S3's
EC50Value, and the hydroxyl radical free radical of ripe Radix Notoginseng S4-S18 removes power EC50Value is substantially less than the EC of raw sangqi ginseng S1-S350Value, says
The antioxidant activity of the ripe Radix Notoginseng of bright the embodiment of the present application preparation is better than the antioxidant activity of raw sangqi ginseng.Wherein, it steams at 120 DEG C
Ripe Radix Notoginseng the sample S15 and S17 that 4h and 8h is obtained have lower DPPH radicals scavenging power EC50Value, illustrates both techniques
The ripe Radix Notoginseng of preparation has stronger DPPH radicals scavenging power.Comparison diagram 1A can be seen that compared with raw sangqi ginseng with Figure 1B, institute
The hydroxyl radical free radical for the ripe Radix Notoginseng sample having removes power EC50The decline degree of value is compared with DPPH radicals scavenging power EC50Value is lower,
Illustrate that compared with DPPH free radical, ripe Radix Notoginseng is more sensitive to hydroxyl radical free radical.Therefore, selection hydroxyl radical free radical removes power size
The evaluation index of condition is steamed as the ripe Radix Notoginseng antioxidant activity of screening.Compared with all samples, the sample of S4-S18 representative
Power EC is removed with relatively low hydroxyl radical free radical50Value, illustrates the antioxygen of ripe Radix Notoginseng sample prepared under these processing procedures
Change effect may be applied in practice.Wherein, ripe Radix Notoginseng sample S12-S18 has more preferably antioxygenic property.Particularly,
Ripe Radix Notoginseng sample S14 and S17 that 2h and 8h is obtained are steamed at 120 DEG C, and there is significant lower hydroxyl radical free radical to remove power EC50Value,
Illustrate that there is the ripe Radix Notoginseng of both techniques preparation significantly stronger hydroxyl radical free radical to remove power.For examining for energy saving aspect
Consider, the ripe Radix Notoginseng that 2h is obtained is steamed at 120 DEG C to reach more preferably antioxidant effect using the less energy.
Although embodiment disclosed by the application is as above, the content only for ease of understanding the application and use
Embodiment is not limited to the application.Technical staff in any the application fields, is taken off not departing from the application
Under the premise of the spirit and scope of dew, any modification and variation, but the application can be carried out in the form and details of implementation
Scope of patent protection, still should be subject to the scope of the claims as defined in the appended claims.
Claims (10)
1. a kind of ripe Radix Notoginseng, by weight percentage, the Panax Notoginseng saponin R contained in the ripe Radix Notoginseng1, ginsenoside Rg1And ginseng
Saponin(e Rb1The total amount of three is 0.35-5.48%, wherein Panax Notoginseng saponin R1Content be 0-1.17%, ginsenoside Rg1Contain
Amount is 0-2.70%, ginsenoside Rb1Content be 0.35-2.10%；The ginsenoside Rh contained in the ripe Radix Notoginseng1, ginseng
Saponin(e Rk3, ginsenoside Rh4, ginsenoside 20 (S)-Rg3With ginsenoside 20 (R)-Rg3Five total amount is 0.26-
6.30%, wherein ginsenoside Rh1Content be 0.06-0.46%, ginsenoside Rk3Content be 0.04-1.71%, ginseng
Saponin(e Rh4Content be 0.17-2.91%, ginsenoside 20 (S)-Rg3Content be 0-0.88%, ginsenoside 20 (R)-Rg3
Content be 0-0.55%；The Panax Notoginseng saponin R contained in the ripe Radix Notoginseng1, ginsenoside Rg1, ginsenoside Rb1, ginsenoside
Rh1, ginsenoside Rk3, ginsenoside Rh4, ginsenoside 20 (S)-Rg3With ginsenoside 20 (R)-Rg3Eight total amount is
5.61-6.66%.
2. ripe Radix Notoginseng according to claim 1, wherein by weight percentage, the Radix Notoginseng soap contained in the ripe Radix Notoginseng
Glycosides R1, ginsenoside Rg1With ginsenoside Rb1The total amount of three is 0.35-2.59%, wherein Panax Notoginseng saponin R1Content be 0-
0.24%, ginsenoside Rg1Content be 0-1.18%, ginsenoside Rb1Content be 0.35-1.17%；In the ripe Radix Notoginseng
The ginsenoside Rh contained1, ginsenoside Rk3, ginsenoside Rh4, ginsenoside 20 (S)-Rg3With ginsenoside 20 (R)-Rg3
Five total amount is 3.02-6.30%, wherein ginsenoside Rh1Content be 0.26-0.46%, ginsenoside Rk3Content
For 0.89-1.71%, ginsenoside Rh4Content be 1.30-2.91%, ginsenoside 20 (S)-Rg3Content be 0.37-
0.88%, ginsenoside 20 (R)-Rg3Content be 0.20-0.55%；The Panax Notoginseng saponin R contained in the ripe Radix Notoginseng1, ginseng
Saponin(e Rg1, ginsenoside Rb1, ginsenoside Rh1, ginsenoside Rk3, ginsenoside Rh4, ginsenoside 20 (S)-Rg3And ginseng
Saponin(e 20 (R)-Rg3Eight total amount is 5.61-6.66%.
3. ripe Radix Notoginseng according to claim 2, by weight percentage, the Panax Notoginseng saponin R contained in the ripe Radix Notoginseng1, people
Join saponin(e Rg1With ginsenoside Rb1The total amount of three is 2.59%, wherein Panax Notoginseng saponin R1Content be 0.24%, ginseng soap
Glycosides Rg1Content be 1.18%, ginsenoside Rb1Content be 1.17%；The ginsenoside Rh contained in the ripe Radix Notoginseng1, people
Join saponin(e Rk3, ginsenoside Rh4, ginsenoside 20 (S)-Rg3With ginsenoside 20 (R)-Rg3Five total amount is 3.02%,
Wherein, ginsenoside Rh1Content be 0.26%, ginsenoside Rk3Content be 0.89%, ginsenoside Rh4Content be
1.30%, ginsenoside 20 (S)-Rg3Content be 0.37%, ginsenoside 20 (R)-Rg3Content be 0.20%；It is described ripe
The Panax Notoginseng saponin R contained in Radix Notoginseng1, ginsenoside Rg1, ginsenoside Rb1, ginsenoside Rh1, ginsenoside Rk3, ginsenoside
Rh4, ginsenoside 20 (S)-Rg3With ginsenoside 20 (R)-Rg3Eight total amount is 5.61%.
4. a kind of method for preparing ripe Radix Notoginseng according to any one of claim 1-3, the method includes the following steps:
Pseudo-ginseng is taken, cleans, dries, is crushed, it is primary to be sieved, obtain Radix Notoginseng powder；
The Radix Notoginseng powder is steamed 2-10 hours at 105-120 DEG C；
It dry, pulverize, secondary sieving, obtain the ripe Radix Notoginseng.
5. according to the method described in claim 4, wherein, the temperature for steaming the Radix Notoginseng powder is 120 DEG C, and the time is 2 hours.
6. the secondary sieving was according to the method described in claim 4, wherein, the primary sieving was 40-60 mesh
40-60 mesh, optionally, the primary sieving was 40 meshes, and the secondary sieving was 40 meshes.
7. according to the method described in claim 4, wherein, the drying is to dry at 45 DEG C to constant weight.
8. the antioxidation application of ripe Radix Notoginseng according to any one of claim 1-3.
9. antioxidation application according to claim 8, wherein directly take the powder of the ripe Radix Notoginseng.
10. antioxidation application according to claim 8, wherein the antioxidation application is that the ripe Radix Notoginseng is anti-in preparation
Purposes in oxidative drug.
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| CN106138141A (en) * | 2016-08-08 | 2016-11-23 | 四川仟源中药饮片有限公司 | A kind of cooked Radix Notoginseng and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106138141A (en) * | 2016-08-08 | 2016-11-23 | 四川仟源中药饮片有限公司 | A kind of cooked Radix Notoginseng and preparation method thereof |
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| Title |
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| 余元勋: "《中国分子中药学》", 31 August 2017, 合肥：安徽科学技术出版社 * |
| 武双等: "HPLC法同时测定生三七和蒸制三七中10种皂苷类成分的含量", 《中药材》 * |
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