JPWO2022039165A5 - - Google Patents

Info

Publication number

JPWO2022039165A5

JPWO2022039165A5 JP2022543959A JP2022543959A JPWO2022039165A5 JP WO2022039165 A5 JPWO2022039165 A5 JP WO2022039165A5 JP 2022543959 A JP2022543959 A JP 2022543959A JP 2022543959 A JP2022543959 A JP 2022543959A JP WO2022039165 A5 JPWO2022039165 A5 JP WO2022039165A5

Authority

JP

Japan

Prior art keywords

cell

region

differentiation

cells

culture substrate

Prior art date

2021-08-17

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Pending

Links

• Espacenet
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• 210000004027 cell Anatomy 0.000 claims 31
• 230000004069 differentiation Effects 0.000 claims 29
• 230000006698 induction Effects 0.000 claims 28
• 238000000034 method Methods 0.000 claims 22
• 239000000758 substrate Substances 0.000 claims 21
• 238000004113 cell culture Methods 0.000 claims 20
• 210000002242 embryoid body Anatomy 0.000 claims 19
• 210000001778 pluripotent stem cell Anatomy 0.000 claims 17
• 230000004663 cell proliferation Effects 0.000 claims 10
• 230000001464 adherent effect Effects 0.000 claims 7
• 238000012258 culturing Methods 0.000 claims 7
• 229920001477 hydrophilic polymer Polymers 0.000 claims 6
• 238000010899 nucleation Methods 0.000 claims 6
• VUDQSRFCCHQIIU-UHFFFAOYSA-N 1-(3,5-dichloro-2,6-dihydroxy-4-methoxyphenyl)hexan-1-one Chemical compound CCCCCC(=O)C1=C(O)C(Cl)=C(OC)C(Cl)=C1O VUDQSRFCCHQIIU-UHFFFAOYSA-N 0.000 claims 5
• 101000942967 Homo sapiens Leukemia inhibitory factor Proteins 0.000 claims 5
• 108010085895 Laminin Proteins 0.000 claims 5
• 102100032352 Leukemia inhibitory factor Human genes 0.000 claims 5
• 230000001939 inductive effect Effects 0.000 claims 5
• 239000003112 inhibitor Substances 0.000 claims 4
• 238000012423 maintenance Methods 0.000 claims 4
• 108010035532 Collagen Proteins 0.000 claims 3
• 102000008186 Collagen Human genes 0.000 claims 3
• 102100037362 Fibronectin Human genes 0.000 claims 3
• 108010067306 Fibronectins Proteins 0.000 claims 3
• 102000007547 Laminin Human genes 0.000 claims 3
• 108010031318 Vitronectin Proteins 0.000 claims 3
• 102100035140 Vitronectin Human genes 0.000 claims 3
• 229920001436 collagen Polymers 0.000 claims 3
• 238000003851 corona treatment Methods 0.000 claims 3
• 108010082117 matrigel Proteins 0.000 claims 3
• 210000003716 mesoderm Anatomy 0.000 claims 3
• 238000009832 plasma treatment Methods 0.000 claims 3
• 239000000126 substance Substances 0.000 claims 3
• 239000012634 fragment Substances 0.000 claims 2
• 210000002490 intestinal epithelial cell Anatomy 0.000 claims 2
• 239000003550 marker Substances 0.000 claims 2
• 108010059616 Activins Proteins 0.000 claims 1
• 102000005606 Activins Human genes 0.000 claims 1
• 102000007350 Bone Morphogenetic Proteins Human genes 0.000 claims 1
• 108010007726 Bone Morphogenetic Proteins Proteins 0.000 claims 1
• 102000001267 GSK3 Human genes 0.000 claims 1
• 108060006662 GSK3 Proteins 0.000 claims 1
• 102000019058 Glycogen Synthase Kinase 3 beta Human genes 0.000 claims 1
• 108010051975 Glycogen Synthase Kinase 3 beta Proteins 0.000 claims 1
• 101000652332 Homo sapiens Transcription factor SOX-1 Proteins 0.000 claims 1
• 101000652324 Homo sapiens Transcription factor SOX-17 Proteins 0.000 claims 1
• 101000641959 Homo sapiens Villin-1 Proteins 0.000 claims 1
• 108010032788 PAX6 Transcription Factor Proteins 0.000 claims 1
• 102100037506 Paired box protein Pax-6 Human genes 0.000 claims 1
• 102100030248 Transcription factor SOX-1 Human genes 0.000 claims 1
• 102100030243 Transcription factor SOX-17 Human genes 0.000 claims 1
• 102000004887 Transforming Growth Factor beta Human genes 0.000 claims 1
• 108090001012 Transforming Growth Factor beta Proteins 0.000 claims 1
• 102100033419 Villin-1 Human genes 0.000 claims 1
• 239000000488 activin Substances 0.000 claims 1
• 229940112869 bone morphogenetic protein Drugs 0.000 claims 1
• 239000002771 cell marker Substances 0.000 claims 1
• 239000011248 coating agent Substances 0.000 claims 1
• 238000000576 coating method Methods 0.000 claims 1
• 230000002860 competitive effect Effects 0.000 claims 1
• 210000002919 epithelial cell Anatomy 0.000 claims 1
• 210000001654 germ layer Anatomy 0.000 claims 1
• 238000004519 manufacturing process Methods 0.000 claims 1
• 210000002161 motor neuron Anatomy 0.000 claims 1
• NFVJNJQRWPQVOA-UHFFFAOYSA-N n-[2-chloro-5-(trifluoromethyl)phenyl]-2-[3-(4-ethyl-5-ethylsulfanyl-1,2,4-triazol-3-yl)piperidin-1-yl]acetamide Chemical compound CCN1C(SCC)=NN=C1C1CN(CC(=O)NC=2C(=CC=C(C=2)C(F)(F)F)Cl)CCC1 NFVJNJQRWPQVOA-UHFFFAOYSA-N 0.000 claims 1
• 210000003061 neural cell Anatomy 0.000 claims 1
• 210000001178 neural stem cell Anatomy 0.000 claims 1
• ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 claims 1