JPWO2020264016A5 - - Google Patents
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- JPWO2020264016A5 JPWO2020264016A5 JP2021576666A JP2021576666A JPWO2020264016A5 JP WO2020264016 A5 JPWO2020264016 A5 JP WO2020264016A5 JP 2021576666 A JP2021576666 A JP 2021576666A JP 2021576666 A JP2021576666 A JP 2021576666A JP WO2020264016 A5 JPWO2020264016 A5 JP WO2020264016A5
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- Prior art keywords
- protein
- sequence
- stranded dna
- exonuclease
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 108020004414 DNA Proteins 0.000 claims 87
- 102000053602 DNA Human genes 0.000 claims 79
- 108090000623 proteins and genes Proteins 0.000 claims 66
- 102000004169 proteins and genes Human genes 0.000 claims 64
- 102000004533 Endonucleases Human genes 0.000 claims 40
- 108010042407 Endonucleases Proteins 0.000 claims 40
- 108060002716 Exonuclease Proteins 0.000 claims 40
- 102000013165 exonuclease Human genes 0.000 claims 40
- 238000000034 method Methods 0.000 claims 40
- 102000052510 DNA-Binding Proteins Human genes 0.000 claims 39
- 101710116602 DNA-Binding protein G5P Proteins 0.000 claims 35
- 101710162453 Replication factor A Proteins 0.000 claims 35
- 101710176758 Replication protein A 70 kDa DNA-binding subunit Proteins 0.000 claims 35
- 101710176276 SSB protein Proteins 0.000 claims 35
- 101710126859 Single-stranded DNA-binding protein Proteins 0.000 claims 35
- 239000000758 substrate Substances 0.000 claims 33
- 210000003527 eukaryotic cell Anatomy 0.000 claims 31
- 210000004027 cell Anatomy 0.000 claims 30
- 108020004682 Single-Stranded DNA Proteins 0.000 claims 26
- 101710163270 Nuclease Proteins 0.000 claims 23
- 108020005004 Guide RNA Proteins 0.000 claims 17
- 238000010362 genome editing Methods 0.000 claims 17
- 108010008532 Deoxyribonuclease I Proteins 0.000 claims 14
- 102000007260 Deoxyribonuclease I Human genes 0.000 claims 14
- 238000000137 annealing Methods 0.000 claims 13
- 238000012986 modification Methods 0.000 claims 13
- 230000004048 modification Effects 0.000 claims 13
- 102000040430 polynucleotide Human genes 0.000 claims 13
- 108091033319 polynucleotide Proteins 0.000 claims 13
- 239000002157 polynucleotide Substances 0.000 claims 13
- 239000013598 vector Substances 0.000 claims 13
- 230000000694 effects Effects 0.000 claims 12
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 10
- 102000039446 nucleic acids Human genes 0.000 claims 10
- 108020004707 nucleic acids Proteins 0.000 claims 10
- 150000007523 nucleic acids Chemical class 0.000 claims 10
- 102000053062 Rad52 DNA Repair and Recombination Human genes 0.000 claims 9
- 108700031762 Rad52 DNA Repair and Recombination Proteins 0.000 claims 9
- 239000003795 chemical substances by application Substances 0.000 claims 9
- 210000000130 stem cell Anatomy 0.000 claims 9
- 108091033409 CRISPR Proteins 0.000 claims 7
- 108700004991 Cas12a Proteins 0.000 claims 7
- 101100316841 Escherichia phage lambda bet gene Proteins 0.000 claims 7
- 229940123611 Genome editing Drugs 0.000 claims 6
- 108010017070 Zinc Finger Nucleases Proteins 0.000 claims 6
- 230000001965 increasing effect Effects 0.000 claims 6
- 108091061403 ERF family Proteins 0.000 claims 5
- 101100355599 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) mus-11 gene Proteins 0.000 claims 5
- 101150006234 RAD52 gene Proteins 0.000 claims 5
- 230000001580 bacterial effect Effects 0.000 claims 5
- 241000588724 Escherichia coli Species 0.000 claims 4
- 108010077850 Nuclear Localization Signals Proteins 0.000 claims 4
- 230000034431 double-strand break repair via homologous recombination Effects 0.000 claims 4
- 108010052305 exodeoxyribonuclease III Proteins 0.000 claims 4
- 230000006780 non-homologous end joining Effects 0.000 claims 4
- 239000002773 nucleotide Substances 0.000 claims 4
- 125000003729 nucleotide group Chemical group 0.000 claims 4
- 230000001568 sexual effect Effects 0.000 claims 4
- 241001515965 unidentified phage Species 0.000 claims 4
- 241000702005 Bacillus phage SPP1 Species 0.000 claims 3
- 108700020911 DNA-Binding Proteins Proteins 0.000 claims 3
- 238000010459 TALEN Methods 0.000 claims 3
- 108010073062 Transcription Activator-Like Effectors Proteins 0.000 claims 3
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims 3
- 238000003776 cleavage reaction Methods 0.000 claims 3
- 230000007017 scission Effects 0.000 claims 3
- 108700014590 single-stranded DNA binding proteins Proteins 0.000 claims 3
- 239000011701 zinc Substances 0.000 claims 3
- 229910052725 zinc Inorganic materials 0.000 claims 3
- 102000020313 Cell-Penetrating Peptides Human genes 0.000 claims 2
- 108010051109 Cell-Penetrating Peptides Proteins 0.000 claims 2
- 108020004635 Complementary DNA Proteins 0.000 claims 2
- 102100033072 DNA replication ATP-dependent helicase DNA2 Human genes 0.000 claims 2
- 241000305071 Enterobacterales Species 0.000 claims 2
- 101000875449 Escherichia phage lambda Exonuclease Proteins 0.000 claims 2
- 108010007577 Exodeoxyribonuclease I Proteins 0.000 claims 2
- 241000702463 Geminiviridae Species 0.000 claims 2
- 101000927313 Homo sapiens DNA replication ATP-dependent helicase DNA2 Proteins 0.000 claims 2
- 241001138401 Kluyveromyces lactis Species 0.000 claims 2
- 108010010677 Phosphodiesterase I Proteins 0.000 claims 2
- 208000020584 Polyploidy Diseases 0.000 claims 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 2
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims 2
- 101000938775 Salmonella phage P22 Essential recombination function protein Proteins 0.000 claims 2
- 241000235347 Schizosaccharomyces pombe Species 0.000 claims 2
- 101710106660 Shutoff alkaline exonuclease Proteins 0.000 claims 2
- 108010043645 Transcription Activator-Like Effector Nucleases Proteins 0.000 claims 2
- 101150023763 UL12 gene Proteins 0.000 claims 2
- 238000010804 cDNA synthesis Methods 0.000 claims 2
- 230000000295 complement effect Effects 0.000 claims 2
- 239000002299 complementary DNA Substances 0.000 claims 2
- 238000012217 deletion Methods 0.000 claims 2
- 230000037430 deletion Effects 0.000 claims 2
- 239000003623 enhancer Substances 0.000 claims 2
- 238000010353 genetic engineering Methods 0.000 claims 2
- 239000001963 growth medium Substances 0.000 claims 2
- 238000003780 insertion Methods 0.000 claims 2
- 230000037431 insertion Effects 0.000 claims 2
- 210000004962 mammalian cell Anatomy 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 230000013011 mating Effects 0.000 claims 2
- 230000001404 mediated effect Effects 0.000 claims 2
- 108020004999 messenger RNA Proteins 0.000 claims 2
- 229920001184 polypeptide Polymers 0.000 claims 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims 2
- 108010068698 spleen exonuclease Proteins 0.000 claims 2
- 238000006467 substitution reaction Methods 0.000 claims 2
- 210000001519 tissue Anatomy 0.000 claims 2
- 241001529453 unidentified herpesvirus Species 0.000 claims 2
- 230000006269 (delayed) early viral mRNA transcription Effects 0.000 claims 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 1
- 241000589158 Agrobacterium Species 0.000 claims 1
- 108091093088 Amplicon Proteins 0.000 claims 1
- 102000008682 Argonaute Proteins Human genes 0.000 claims 1
- 108010088141 Argonaute Proteins Proteins 0.000 claims 1
- 108020004638 Circular DNA Proteins 0.000 claims 1
- 108091026890 Coding region Proteins 0.000 claims 1
- 101710096438 DNA-binding protein Proteins 0.000 claims 1
- 241000147019 Enterobacter sp. Species 0.000 claims 1
- 241000587112 Enterobacteriaceae sp. Species 0.000 claims 1
- 101000667879 Escherichia phage lambda Recombination protein bet Proteins 0.000 claims 1
- 241000488157 Escherichia sp. Species 0.000 claims 1
- 241000588754 Klebsiella sp. Species 0.000 claims 1
- 241000983368 Pantoea sp. Species 0.000 claims 1
- 108091005804 Peptidases Proteins 0.000 claims 1
- 108010076039 Polyproteins Proteins 0.000 claims 1
- 239000004365 Protease Substances 0.000 claims 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 1
- 241000607714 Serratia sp. Species 0.000 claims 1
- 241000607758 Shigella sp. Species 0.000 claims 1
- 241000131891 Yersinia sp. Species 0.000 claims 1
- 210000004102 animal cell Anatomy 0.000 claims 1
- 210000000349 chromosome Anatomy 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 201000010099 disease Diseases 0.000 claims 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- 235000003869 genetically modified organism Nutrition 0.000 claims 1
- 210000004602 germ cell Anatomy 0.000 claims 1
- 210000002980 germ line cell Anatomy 0.000 claims 1
- 230000001939 inductive effect Effects 0.000 claims 1
- 239000013612 plasmid Substances 0.000 claims 1
- 239000002243 precursor Substances 0.000 claims 1
- 238000012545 processing Methods 0.000 claims 1
- 230000001172 regenerating effect Effects 0.000 claims 1
- 238000012163 sequencing technique Methods 0.000 claims 1
- 238000001890 transfection Methods 0.000 claims 1
- 230000009466 transformation Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962866317P | 2019-06-25 | 2019-06-25 | |
| US62/866,317 | 2019-06-25 | ||
| PCT/US2020/039410 WO2020264016A1 (en) | 2019-06-25 | 2020-06-24 | Improved homology dependent repair genome editing |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2022539338A JP2022539338A (ja) | 2022-09-08 |
| JPWO2020264016A5 true JPWO2020264016A5 (https=) | 2023-07-04 |
| JP2022539338A5 JP2022539338A5 (https=) | 2023-07-04 |
Family
ID=71579688
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021576666A Pending JP2022539338A (ja) | 2019-06-25 | 2020-06-24 | 改良された相同性依存性修復ゲノム編集 |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US11041172B2 (https=) |
| EP (1) | EP3990633A1 (https=) |
| JP (1) | JP2022539338A (https=) |
| KR (1) | KR20220052905A (https=) |
| CN (2) | CN120665955A (https=) |
| AR (1) | AR123323A1 (https=) |
| AU (1) | AU2020301236A1 (https=) |
| BR (1) | BR112021026220A2 (https=) |
| CA (1) | CA3138663A1 (https=) |
| IL (1) | IL289106A (https=) |
| MX (1) | MX2021014861A (https=) |
| SG (1) | SG11202112092TA (https=) |
| WO (1) | WO2020264016A1 (https=) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US12139716B2 (en) * | 2016-12-21 | 2024-11-12 | Lin Lung Jr | Composition for editing a nucleic acid sequence and method using the same |
| CN109666693B (zh) * | 2018-12-29 | 2022-08-16 | 北京市农林科学院 | Mg132在碱基编辑系统编辑受体基因组中的应用 |
| MX2021014861A (es) * | 2019-06-25 | 2022-06-22 | Inari Agriculture Tech Inc | Edicion genomica de reparacion dependiente de homologia mejorada. |
| WO2021041846A1 (en) | 2019-08-30 | 2021-03-04 | Inari Agriculture, Inc. | Rna-guided nucleases and dna binding proteins |
| US20220372522A1 (en) * | 2019-10-08 | 2022-11-24 | Life Technologies Corporation | Compositions and methods for homology-directed recombination |
| US11242534B1 (en) | 2020-07-31 | 2022-02-08 | Inari Agriculture Technology, Inc. | INHT31 transgenic soybean |
| US20230265445A1 (en) * | 2020-07-31 | 2023-08-24 | Inari Agriculture Technology, Inc. | Removable plant transgenic loci with cognate guide rna recognition sites |
| US20220030822A1 (en) * | 2020-07-31 | 2022-02-03 | Inari Agriculture Technology, Inc. | Inht26 transgenic soybean |
| US12529062B2 (en) | 2020-07-31 | 2026-01-20 | Inari Agriculture Technology, Inc. | INIR12 transgenic maize |
| US11214811B1 (en) | 2020-07-31 | 2022-01-04 | Inari Agriculture Technology, Inc. | INIR6 transgenic maize |
| US20240011043A1 (en) | 2020-07-31 | 2024-01-11 | Inari Agriculture Technology, Inc. | Generation of plants with improved transgenic loci by genome editing |
| EP4243608A1 (en) * | 2020-11-11 | 2023-09-20 | Leibniz-Institut für Pflanzenbiochemie (IPB) | Fusion protein for editing endogenous dna of a eukaryotic cell |
| MX2023012044A (es) * | 2021-04-13 | 2024-01-05 | Arsenal Biosciences Inc | Union de extremos mediada por homologia no viral. |
| EP4453181A4 (en) * | 2021-12-20 | 2025-12-24 | Aldevron Llc | GENE THERAPY VECTOR PRODUCTION IN MODIFIED BACTERIA |
| EP4479530A1 (en) * | 2022-02-18 | 2024-12-25 | Pulmobiotics, S.L. | Systems, tools, and methods for engineering bacteria |
| CN116286741A (zh) * | 2022-03-03 | 2023-06-23 | 清华大学 | 5’→3’核酸外切酶在基因编辑系统中的用途和基因编辑系统及其编辑方法 |
| WO2023192825A1 (en) * | 2022-03-29 | 2023-10-05 | Inari Agriculture Technology, Inc. | Targeted donor dna insertion and indel editing of plant genes |
| CN115960733B (zh) * | 2022-09-19 | 2023-11-24 | 苏州泓迅生物科技股份有限公司 | 一种用于大片段dna组装的基因工程酵母菌、构建方法及其应用 |
| CN115976086B (zh) * | 2023-01-19 | 2023-08-08 | 河南农业大学 | 一种细菌CRISPR-Cas9基因编辑的方法和应用 |
| WO2024168253A1 (en) * | 2023-02-10 | 2024-08-15 | Possible Medicines Llc | Delivery of an rna guided recombination system |
| WO2024168265A1 (en) * | 2023-02-10 | 2024-08-15 | Possible Medicines Llc | Aav delivery of rna guided recombination system |
| CN116376961A (zh) * | 2023-04-24 | 2023-07-04 | 深圳大学 | 同源重组酶在莱茵衣藻基因组编辑中的应用 |
| CN116873928B (zh) * | 2023-06-28 | 2025-08-05 | 浙江工业大学 | 一种碳化钨纳米棒-碳纳米片复合材料、铁碳微电极材料的制备方法和应用 |
| CN116555346B (zh) * | 2023-07-05 | 2023-09-01 | 中山大学 | 一种采用碳纳米管基因载体递送系统促进草鱼生长的方法 |
| KR20250065458A (ko) * | 2023-11-03 | 2025-05-13 | 한경국립대학교 산학협력단 | 벼에서 CRISPR/Cas9-제미니바이러스 레플리콘 기반의 상동지정 복구 효율 증진 방법 |
| CN117487959B (zh) * | 2024-01-02 | 2024-03-22 | 东北农业大学 | 一种与印度南瓜侧枝数量相关基因连锁的分子标记及应用 |
| WO2025179057A1 (en) * | 2024-02-21 | 2025-08-28 | The Regents Of The University Of California | In vivo modification of cell genomes |
| CN120796332B (zh) * | 2025-09-12 | 2026-01-09 | 中国科学院深圳先进技术研究院 | 一种基因编辑系统、双质粒系统、工程菌及应用 |
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2020
- 2020-06-24 MX MX2021014861A patent/MX2021014861A/es unknown
- 2020-06-24 US US16/911,156 patent/US11041172B2/en active Active
- 2020-06-24 JP JP2021576666A patent/JP2022539338A/ja active Pending
- 2020-06-24 BR BR112021026220A patent/BR112021026220A2/pt unknown
- 2020-06-24 WO PCT/US2020/039410 patent/WO2020264016A1/en not_active Ceased
- 2020-06-24 CN CN202510310885.3A patent/CN120665955A/zh active Pending
- 2020-06-24 AU AU2020301236A patent/AU2020301236A1/en active Pending
- 2020-06-24 SG SG11202112092TA patent/SG11202112092TA/en unknown
- 2020-06-24 CN CN202080044833.1A patent/CN114630910A/zh active Pending
- 2020-06-24 CA CA3138663A patent/CA3138663A1/en active Pending
- 2020-06-24 EP EP20739829.8A patent/EP3990633A1/en active Pending
- 2020-06-24 KR KR1020227002110A patent/KR20220052905A/ko active Pending
- 2020-06-25 AR ARP200101810A patent/AR123323A1/es not_active Application Discontinuation
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2021
- 2021-05-21 US US17/326,891 patent/US20210277422A1/en active Pending
- 2021-12-19 IL IL289106A patent/IL289106A/en unknown
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