AU2020301236A1 - Improved homology dependent repair genome editing - Google Patents
Improved homology dependent repair genome editing Download PDFInfo
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- AU2020301236A1 AU2020301236A1 AU2020301236A AU2020301236A AU2020301236A1 AU 2020301236 A1 AU2020301236 A1 AU 2020301236A1 AU 2020301236 A AU2020301236 A AU 2020301236A AU 2020301236 A AU2020301236 A AU 2020301236A AU 2020301236 A1 AU2020301236 A1 AU 2020301236A1
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- protein
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- exonuclease
- sequence
- stranded dna
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962866317P | 2019-06-25 | 2019-06-25 | |
| US62/866,317 | 2019-06-25 | ||
| PCT/US2020/039410 WO2020264016A1 (en) | 2019-06-25 | 2020-06-24 | Improved homology dependent repair genome editing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2020301236A1 true AU2020301236A1 (en) | 2021-11-25 |
Family
ID=71579688
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2020301236A Pending AU2020301236A1 (en) | 2019-06-25 | 2020-06-24 | Improved homology dependent repair genome editing |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US11041172B2 (https=) |
| EP (1) | EP3990633A1 (https=) |
| JP (1) | JP2022539338A (https=) |
| KR (1) | KR20220052905A (https=) |
| CN (2) | CN120665955A (https=) |
| AR (1) | AR123323A1 (https=) |
| AU (1) | AU2020301236A1 (https=) |
| BR (1) | BR112021026220A2 (https=) |
| CA (1) | CA3138663A1 (https=) |
| IL (1) | IL289106A (https=) |
| MX (1) | MX2021014861A (https=) |
| SG (1) | SG11202112092TA (https=) |
| WO (1) | WO2020264016A1 (https=) |
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| MX2021014861A (es) * | 2019-06-25 | 2022-06-22 | Inari Agriculture Tech Inc | Edicion genomica de reparacion dependiente de homologia mejorada. |
| WO2021041846A1 (en) | 2019-08-30 | 2021-03-04 | Inari Agriculture, Inc. | Rna-guided nucleases and dna binding proteins |
| US20220372522A1 (en) * | 2019-10-08 | 2022-11-24 | Life Technologies Corporation | Compositions and methods for homology-directed recombination |
| US11242534B1 (en) | 2020-07-31 | 2022-02-08 | Inari Agriculture Technology, Inc. | INHT31 transgenic soybean |
| US20230265445A1 (en) * | 2020-07-31 | 2023-08-24 | Inari Agriculture Technology, Inc. | Removable plant transgenic loci with cognate guide rna recognition sites |
| US20220030822A1 (en) * | 2020-07-31 | 2022-02-03 | Inari Agriculture Technology, Inc. | Inht26 transgenic soybean |
| US12529062B2 (en) | 2020-07-31 | 2026-01-20 | Inari Agriculture Technology, Inc. | INIR12 transgenic maize |
| US11214811B1 (en) | 2020-07-31 | 2022-01-04 | Inari Agriculture Technology, Inc. | INIR6 transgenic maize |
| US20240011043A1 (en) | 2020-07-31 | 2024-01-11 | Inari Agriculture Technology, Inc. | Generation of plants with improved transgenic loci by genome editing |
| EP4243608A1 (en) * | 2020-11-11 | 2023-09-20 | Leibniz-Institut für Pflanzenbiochemie (IPB) | Fusion protein for editing endogenous dna of a eukaryotic cell |
| MX2023012044A (es) * | 2021-04-13 | 2024-01-05 | Arsenal Biosciences Inc | Union de extremos mediada por homologia no viral. |
| EP4453181A4 (en) * | 2021-12-20 | 2025-12-24 | Aldevron Llc | GENE THERAPY VECTOR PRODUCTION IN MODIFIED BACTERIA |
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| CN116286741A (zh) * | 2022-03-03 | 2023-06-23 | 清华大学 | 5’→3’核酸外切酶在基因编辑系统中的用途和基因编辑系统及其编辑方法 |
| WO2023192825A1 (en) * | 2022-03-29 | 2023-10-05 | Inari Agriculture Technology, Inc. | Targeted donor dna insertion and indel editing of plant genes |
| CN115960733B (zh) * | 2022-09-19 | 2023-11-24 | 苏州泓迅生物科技股份有限公司 | 一种用于大片段dna组装的基因工程酵母菌、构建方法及其应用 |
| CN115976086B (zh) * | 2023-01-19 | 2023-08-08 | 河南农业大学 | 一种细菌CRISPR-Cas9基因编辑的方法和应用 |
| WO2024168253A1 (en) * | 2023-02-10 | 2024-08-15 | Possible Medicines Llc | Delivery of an rna guided recombination system |
| WO2024168265A1 (en) * | 2023-02-10 | 2024-08-15 | Possible Medicines Llc | Aav delivery of rna guided recombination system |
| CN116376961A (zh) * | 2023-04-24 | 2023-07-04 | 深圳大学 | 同源重组酶在莱茵衣藻基因组编辑中的应用 |
| CN116873928B (zh) * | 2023-06-28 | 2025-08-05 | 浙江工业大学 | 一种碳化钨纳米棒-碳纳米片复合材料、铁碳微电极材料的制备方法和应用 |
| CN116555346B (zh) * | 2023-07-05 | 2023-09-01 | 中山大学 | 一种采用碳纳米管基因载体递送系统促进草鱼生长的方法 |
| KR20250065458A (ko) * | 2023-11-03 | 2025-05-13 | 한경국립대학교 산학협력단 | 벼에서 CRISPR/Cas9-제미니바이러스 레플리콘 기반의 상동지정 복구 효율 증진 방법 |
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| US11041172B2 (en) | 2021-06-22 |
| CN114630910A (zh) | 2022-06-14 |
| WO2020264016A1 (en) | 2020-12-30 |
| BR112021026220A2 (pt) | 2022-02-15 |
| IL289106A (en) | 2022-02-01 |
| US20200407754A1 (en) | 2020-12-31 |
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