CN114630910A - 改善的同源依赖修复基因组编辑 - Google Patents

改善的同源依赖修复基因组编辑 Download PDF

Info

Publication number
CN114630910A
CN114630910A CN202080044833.1A CN202080044833A CN114630910A CN 114630910 A CN114630910 A CN 114630910A CN 202080044833 A CN202080044833 A CN 202080044833A CN 114630910 A CN114630910 A CN 114630910A
Authority
CN
China
Prior art keywords
protein
cell
exonuclease
sequence
stranded dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202080044833.1A
Other languages
English (en)
Chinese (zh)
Inventor
T·塞尔玛克
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Inari Agricultural Technology Co ltd
Original Assignee
Inari Agricultural Technology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inari Agricultural Technology Co ltd filed Critical Inari Agricultural Technology Co ltd
Priority to CN202510310885.3A priority Critical patent/CN120665955A/zh
Publication of CN114630910A publication Critical patent/CN114630910A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202080044833.1A 2019-06-25 2020-06-24 改善的同源依赖修复基因组编辑 Pending CN114630910A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202510310885.3A CN120665955A (zh) 2019-06-25 2020-06-24 改善的同源依赖修复基因组编辑

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962866317P 2019-06-25 2019-06-25
US62/866,317 2019-06-25
PCT/US2020/039410 WO2020264016A1 (en) 2019-06-25 2020-06-24 Improved homology dependent repair genome editing

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN202510310885.3A Division CN120665955A (zh) 2019-06-25 2020-06-24 改善的同源依赖修复基因组编辑

Publications (1)

Publication Number Publication Date
CN114630910A true CN114630910A (zh) 2022-06-14

Family

ID=71579688

Family Applications (2)

Application Number Title Priority Date Filing Date
CN202080044833.1A Pending CN114630910A (zh) 2019-06-25 2020-06-24 改善的同源依赖修复基因组编辑
CN202510310885.3A Pending CN120665955A (zh) 2019-06-25 2020-06-24 改善的同源依赖修复基因组编辑

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN202510310885.3A Pending CN120665955A (zh) 2019-06-25 2020-06-24 改善的同源依赖修复基因组编辑

Country Status (13)

Country Link
US (2) US11041172B2 (https=)
EP (1) EP3990633A1 (https=)
JP (1) JP2022539338A (https=)
KR (1) KR20220052905A (https=)
CN (2) CN114630910A (https=)
AR (1) AR123323A1 (https=)
AU (1) AU2020301236A1 (https=)
BR (1) BR112021026220A2 (https=)
CA (1) CA3138663A1 (https=)
IL (1) IL289106A (https=)
MX (1) MX2021014861A (https=)
SG (1) SG11202112092TA (https=)
WO (1) WO2020264016A1 (https=)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109666693A (zh) * 2018-12-29 2019-04-23 北京市农林科学院 Mg132在碱基编辑系统编辑受体基因组中的应用
CN116873928A (zh) * 2023-06-28 2023-10-13 浙江工业大学 一种碳化钨纳米棒-碳纳米片复合材料、铁碳微电极材料的制备方法和应用
CN120796332A (zh) * 2025-09-12 2025-10-17 中国科学院深圳先进技术研究院 一种基因编辑系统、双质粒系统、工程菌及应用

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018119021A1 (en) * 2016-12-21 2018-06-28 HSU, Ethan Composition for editing a nucleic acid sequence and method using the same
CN114630910A (zh) * 2019-06-25 2022-06-14 伊纳瑞农业技术有限公司 改善的同源依赖修复基因组编辑
WO2021041846A1 (en) 2019-08-30 2021-03-04 Inari Agriculture, Inc. Rna-guided nucleases and dna binding proteins
US20220372522A1 (en) * 2019-10-08 2022-11-24 Life Technologies Corporation Compositions and methods for homology-directed recombination
US11242534B1 (en) 2020-07-31 2022-02-08 Inari Agriculture Technology, Inc. INHT31 transgenic soybean
US11214811B1 (en) 2020-07-31 2022-01-04 Inari Agriculture Technology, Inc. INIR6 transgenic maize
US20240011043A1 (en) 2020-07-31 2024-01-11 Inari Agriculture Technology, Inc. Generation of plants with improved transgenic loci by genome editing
US20230265445A1 (en) * 2020-07-31 2023-08-24 Inari Agriculture Technology, Inc. Removable plant transgenic loci with cognate guide rna recognition sites
US20220030822A1 (en) * 2020-07-31 2022-02-03 Inari Agriculture Technology, Inc. Inht26 transgenic soybean
US12529062B2 (en) 2020-07-31 2026-01-20 Inari Agriculture Technology, Inc. INIR12 transgenic maize
WO2022101286A1 (en) * 2020-11-11 2022-05-19 Leibniz-Institut Für Pflanzenbiochemie Fusion protein for editing endogenous dna of a eukaryotic cell
CN117355607A (zh) * 2021-04-13 2024-01-05 阿森纳生物科学公司 非病毒同源性介导的末端连接
KR20240128889A (ko) * 2021-12-20 2024-08-27 알데브론 엘엘씨 조작된 박테리아에서 유전자 치료 벡터의 생산
WO2023156585A1 (en) * 2022-02-18 2023-08-24 Pulmobiotics, S.L. Systems, tools, and methods for engineering bacteria
CN116286741A (zh) * 2022-03-03 2023-06-23 清华大学 5’→3’核酸外切酶在基因编辑系统中的用途和基因编辑系统及其编辑方法
WO2023192825A1 (en) * 2022-03-29 2023-10-05 Inari Agriculture Technology, Inc. Targeted donor dna insertion and indel editing of plant genes
CN115960733B (zh) * 2022-09-19 2023-11-24 苏州泓迅生物科技股份有限公司 一种用于大片段dna组装的基因工程酵母菌、构建方法及其应用
CN115976086B (zh) * 2023-01-19 2023-08-08 河南农业大学 一种细菌CRISPR-Cas9基因编辑的方法和应用
WO2024168265A1 (en) * 2023-02-10 2024-08-15 Possible Medicines Llc Aav delivery of rna guided recombination system
WO2024168253A1 (en) * 2023-02-10 2024-08-15 Possible Medicines Llc Delivery of an rna guided recombination system
CN116376961A (zh) * 2023-04-24 2023-07-04 深圳大学 同源重组酶在莱茵衣藻基因组编辑中的应用
CN116555346B (zh) * 2023-07-05 2023-09-01 中山大学 一种采用碳纳米管基因载体递送系统促进草鱼生长的方法
KR20250065458A (ko) * 2023-11-03 2025-05-13 한경국립대학교 산학협력단 벼에서 CRISPR/Cas9-제미니바이러스 레플리콘 기반의 상동지정 복구 효율 증진 방법
CN117487959B (zh) * 2024-01-02 2024-03-22 东北农业大学 一种与印度南瓜侧枝数量相关基因连锁的分子标记及应用
WO2025179057A1 (en) * 2024-02-21 2025-08-28 The Regents Of The University Of California In vivo modification of cell genomes

Family Cites Families (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US39247A (en) 1863-07-14 Improvement in skates
US5352605A (en) 1983-01-17 1994-10-04 Monsanto Company Chimeric genes for transforming plant cells using viral promoters
US5428147A (en) 1983-04-15 1995-06-27 Mycogen Plant Science, Inc. Octopine T-DNA promoters
US5322938A (en) 1987-01-13 1994-06-21 Monsanto Company DNA sequence for enhancing the efficiency of transcription
US5310667A (en) 1989-07-17 1994-05-10 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases
US5641876A (en) 1990-01-05 1997-06-24 Cornell Research Foundation, Inc. Rice actin gene and promoter
PL295547A1 (https=) 1990-04-26 1992-10-05 Plant Genetic Systems Nv
US5633435A (en) 1990-08-31 1997-05-27 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
US5866775A (en) 1990-09-28 1999-02-02 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases
US5277905A (en) 1991-01-16 1994-01-11 Mycogen Corporation Coleopteran-active bacillus thuringiensis isolate
DE69227911T2 (de) 1991-08-02 1999-05-12 Kubota Corp., Tokio/Tokyo Neuer mikroorganismus und insektizid
AU5676394A (en) 1992-11-20 1994-06-22 Agracetus, Inc. Transgenic cotton plants producing heterologous bioplastic
CA2161881C (en) 1993-01-13 2001-03-27 A. Gururaj Rao High lysine derivatives of alpha-hordothionin
RU2165411C2 (ru) 1994-02-04 2001-04-20 Басф Акциенгезельшафт Производные фенилуксусной кислоты, промежуточные продукты для их получения и содержащие их средства
US5593881A (en) 1994-05-06 1997-01-14 Mycogen Corporation Bacillus thuringiensis delta-endotoxin
US5792931A (en) 1994-08-12 1998-08-11 Pioneer Hi-Bred International, Inc. Fumonisin detoxification compositions and methods
CA2160529A1 (en) 1994-10-14 1996-04-15 Toshihiko Iizuka Bacillus strain and harmful organism controlling agents
EP0828835A1 (en) 1995-06-02 1998-03-18 Pioneer Hi-Bred International, Inc. HIGH THREONINE DERIVATIVES OF $g(a)-HORDOTHIONIN
PL323635A1 (en) 1995-06-02 1998-04-14 Pioneer Hi Bred Int Derivatives of alpha-hordothionine of high methionine content
US5703049A (en) 1996-02-29 1997-12-30 Pioneer Hi-Bred Int'l, Inc. High methionine derivatives of α-hordothionin for pathogen-control
US5850016A (en) 1996-03-20 1998-12-15 Pioneer Hi-Bred International, Inc. Alteration of amino acid compositions in seeds
CA2270289C (en) 1996-11-01 2005-09-27 Pioneer Hi-Bred International, Inc. Proteins with enhanced levels of essential amino acids
ES2341926T3 (es) 1998-03-02 2010-06-29 Massachusetts Institute Of Technology Poliproteinas con dedos de cinc que tienen enlazadores mejorados.
US6453242B1 (en) 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US6794136B1 (en) 2000-11-20 2004-09-21 Sangamo Biosciences, Inc. Iterative optimization in the design of binding proteins
JP2003528571A (ja) 1999-04-29 2003-09-30 シンジェンタ リミテッド 除草剤抵抗性植物
US20020192813A1 (en) 1999-08-18 2002-12-19 Timothy W. Conner Plant expression vectors
US6683231B2 (en) 2000-06-02 2004-01-27 E. I. Du Pont De Nemours And Company High level production of p-hydroxybenzoic acid in green plants
BR0114322A (pt) 2000-09-29 2004-06-15 Syngenta Ltd Enzima de epsps resistente a glifosato, polinucleotìdeo isolado, vetor, material de plantas, plantas completas férteis, morfologicamente normais, soja, canola, brassica, algodão, beterraba sacarina, girassol, ervilhas, batatas e beterrabas de forragem, métodos para controlar seletivamente ervas daninhas em um campo, e para produzir plantas que sejam substancialmente tolerantes ou substancialmente resistentes a herbicida de glifosato, uso do polinucleotìdeo, métodos para selecionar material biológico transformado de modo a expressar um gene de interesse, e para regenerar uma planta fértil transformada para conter dna estranho, e, kit diagnóstico
US7151204B2 (en) 2001-01-09 2006-12-19 Monsanto Technology Llc Maize chloroplast aldolase promoter compositions and methods for use thereof
US7045684B1 (en) 2002-08-19 2006-05-16 Mertec, Llc Glyphosate-resistant plants
NZ574568A (en) 2006-08-11 2011-04-29 Monsanto Technology Llc Production of high tryptophan maize by chloroplast targeted expression of anthranilate synthase
EP2300617B1 (en) 2008-07-16 2016-03-23 Monsanto Technology LLC Methods and vectors for producing transgenic plants
TWI576432B (zh) 2009-04-07 2017-04-01 陶氏農業科學公司 序列特異性核酸酶之奈米粒子媒介式遞送技術
MX349747B (es) 2009-10-06 2017-08-09 Univ California Generacion de plantas haploides y cruza de plantas mejorada.
AU2010307119B2 (en) 2009-10-16 2015-04-30 Corteva Agriscience Llc Use of dendrimer nanotechnology for delivery of biomolecules into plant cells
NZ602139A (en) 2010-03-31 2015-01-30 Dow Agrosciences Llc Plant peptide gamma-zein for delivery of biomolecules into plant cells
GB201009732D0 (en) 2010-06-10 2010-07-21 Gene Bridges Gmbh Direct cloning
UA113046C2 (xx) 2010-07-07 2016-12-12 Спосіб введення лінійної молекули нуклеїнової кислоти в клітину рослини, яка має клітинну стінку, із застосуванням пегильованих напівпровідникових наночастинок
EP2697379B1 (en) 2011-03-23 2018-08-29 Dow AgroSciences LLC Quantum dot carrier peptide conjugates suitable for imaging and delivery applications in plants
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
PT2768942T (pt) 2011-10-17 2020-01-21 Massachusetts Inst Technology Entrega intracelular
US20130145488A1 (en) 2011-12-06 2013-06-06 Iowa State University Research Foundation, Inc. Mesoporous silica nanoparticles suitable for co-delivery
US20130185823A1 (en) 2012-01-16 2013-07-18 Academia Sinica Mesoporous silica nanoparticle-mediated delivery of dna into arabidopsis root
AU2013266968B2 (en) 2012-05-25 2017-06-29 Emmanuelle CHARPENTIER Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription
US20140096284A1 (en) 2012-10-01 2014-04-03 Iowa State University Research Foundation, Inc. Method for the delivery of molecules lyophilized onto microparticles to plant tissues
AU2013335451C1 (en) 2012-10-23 2024-07-04 Toolgen Incorporated Composition for cleaving a target DNA comprising a guide RNA specific for the target DNA and Cas protein-encoding nucleic acid or Cas protein, and use thereof
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
US20140310830A1 (en) 2012-12-12 2014-10-16 Feng Zhang CRISPR-Cas Nickase Systems, Methods And Compositions For Sequence Manipulation in Eukaryotes
CN113355357B (zh) 2012-12-12 2024-12-03 布罗德研究所有限公司 对用于序列操纵的改进的系统、方法和酶组合物进行的工程化和优化
WO2014093655A2 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains
IL239344B2 (en) 2012-12-12 2024-06-01 Broad Inst Inc Systems engineering, methods and optimal guiding components for sequence manipulation
EP2840140B2 (en) 2012-12-12 2023-02-22 The Broad Institute, Inc. Crispr-Cas based method for mutation of prokaryotic cells
EP2951295B1 (en) 2013-02-01 2018-08-01 Cellectis Tevi chimeric endonucleases and their preferential cleavage sites
US9677082B2 (en) 2013-03-15 2017-06-13 Syngenta Participations Ag Haploid induction compositions and methods for use therefor
US9902973B2 (en) 2013-04-11 2018-02-27 Caribou Biosciences, Inc. Methods of modifying a target nucleic acid with an argonaute
US20150040268A1 (en) 2013-04-25 2015-02-05 Morflora Israel Ltd Methods and compositions for the delivery of nucleic acids to seeds
AU2014275045A1 (en) 2013-06-03 2015-12-17 University Of Southern California Targeted crosslinked multilamellar liposomes
JP2016521561A (ja) 2013-06-14 2016-07-25 セレクティス 植物における非トランスジェニックのゲノム編集のための方法
US9944925B2 (en) 2013-08-02 2018-04-17 Enevolv, Inc. Processes and host cells for genome, pathway, and biomolecular engineering
WO2015021266A1 (en) 2013-08-09 2015-02-12 Massachusetts Institute Of Technology Nanobionic engineering of organelles and photosynthetic organisms
CN120574876A (zh) 2013-08-22 2025-09-02 纳幕尔杜邦公司 使用向导rna/cas内切核酸酶系统的植物基因组修饰及其使用方法
US9675078B2 (en) 2013-10-15 2017-06-13 Board Of Trustees Of The University Of Arkansas Method of delivery of bio-active agents to plant cells by using nano-sized materials as carriers
ES2898460T3 (es) 2014-02-27 2022-03-07 Monsanto Technology Llc Composiciones y procedimientos para la modificación genómica dirigida al sitio
ES2781323T3 (es) 2014-06-23 2020-09-01 Regeneron Pharma Montaje de ADN mediado por nucleasas
US10676754B2 (en) 2014-07-11 2020-06-09 E I Du Pont De Nemours And Company Compositions and methods for producing plants resistant to glyphosate herbicide
WO2016025759A1 (en) 2014-08-14 2016-02-18 Shen Yuelei Dna knock-in system
EP3201340B1 (en) 2014-10-01 2020-12-02 The General Hospital Corporation Methods for increasing efficiency of nuclease-induced homology-directed repair
EP4464338A3 (en) * 2014-11-07 2025-02-12 Editas Medicine, Inc. Systems for improving crispr/cas-mediated genome-editing
WO2016100955A2 (en) 2014-12-20 2016-06-23 Identifygenomics, Llc Compositions and methods for targeted depletion, enrichment, and partitioning of nucleic acids using crispr/cas system proteins
US10155938B2 (en) 2015-04-14 2018-12-18 City Of Hope Coexpression of CAS9 and TREX2 for targeted mutagenesis
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
EP3353296B1 (en) 2015-09-24 2020-11-04 Editas Medicine, Inc. Use of exonucleases to improve crispr/cas-mediated genome editing
BR112018008705B1 (pt) 2015-10-30 2023-12-26 Pioneer Hi-Bred International, Inc Método para produzir uma planta transgênica
US20170175140A1 (en) 2015-12-16 2017-06-22 Regents Of The University Of Minnesota Methods for using a 5'-exonuclease to increase homologous recombination in eukaryotic cells
WO2017184227A2 (en) 2016-02-04 2017-10-26 President And Fellows Of Harvard College Recombinase genome editing
WO2018067846A1 (en) 2016-10-05 2018-04-12 President And Fellows Of Harvard College Methods of crispr mediated genome modulation in v. natriegens
US20190264218A1 (en) 2016-11-04 2019-08-29 Flagship Pioneering Innovations V, Inc. Novel Plant Cells, Plants, and Seeds
CN108085328B (zh) * 2016-11-21 2021-06-22 中国科学院分子植物科学卓越创新中心 一种dna序列编辑的方法
EP3728589A4 (en) 2017-12-22 2021-11-03 G+Flas Life Sciences CHEMICAL GENOMIC ENGINEERING MOLECULES AND PROCESSES
WO2020003311A1 (en) 2018-06-25 2020-01-02 Yeda Research And Development Co. Ltd. Systems and methods for increasing efficiency of genome editing
WO2020041172A1 (en) 2018-08-21 2020-02-27 The Jackson Laboratory Methods and compositions for recruiting dna repair proteins
CN114630910A (zh) * 2019-06-25 2022-06-14 伊纳瑞农业技术有限公司 改善的同源依赖修复基因组编辑

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109666693A (zh) * 2018-12-29 2019-04-23 北京市农林科学院 Mg132在碱基编辑系统编辑受体基因组中的应用
CN109666693B (zh) * 2018-12-29 2022-08-16 北京市农林科学院 Mg132在碱基编辑系统编辑受体基因组中的应用
CN116873928A (zh) * 2023-06-28 2023-10-13 浙江工业大学 一种碳化钨纳米棒-碳纳米片复合材料、铁碳微电极材料的制备方法和应用
CN120796332A (zh) * 2025-09-12 2025-10-17 中国科学院深圳先进技术研究院 一种基因编辑系统、双质粒系统、工程菌及应用
CN120796332B (zh) * 2025-09-12 2026-01-09 中国科学院深圳先进技术研究院 一种基因编辑系统、双质粒系统、工程菌及应用

Also Published As

Publication number Publication date
AU2020301236A1 (en) 2021-11-25
KR20220052905A (ko) 2022-04-28
IL289106A (en) 2022-02-01
US20200407754A1 (en) 2020-12-31
WO2020264016A1 (en) 2020-12-30
SG11202112092TA (en) 2021-11-29
BR112021026220A2 (pt) 2022-02-15
AR123323A1 (es) 2022-11-23
MX2021014861A (es) 2022-06-22
CN120665955A (zh) 2025-09-19
EP3990633A1 (en) 2022-05-04
CA3138663A1 (en) 2020-12-30
US11041172B2 (en) 2021-06-22
US20210277422A1 (en) 2021-09-09
JP2022539338A (ja) 2022-09-08

Similar Documents

Publication Publication Date Title
US11041172B2 (en) Homology dependent repair genome editing
US20230242927A1 (en) Novel plant cells, plants, and seeds
US12209246B2 (en) Methods of genetically altering a whole plant seed
EP3036333B2 (en) Methods for producing genetic modifications in a plant genome without incorporating a selectable transgene marker, and compositions thereof
US20230235349A1 (en) Novel maize cells and maize plants
EP3728577A1 (en) Plant gene editing systems, methods, and compositions
US11976291B2 (en) Genetically enhanced maize plants
US11802288B1 (en) Methods for efficient soybean genome editing
US11788098B2 (en) Plant transformation
US20260078391A1 (en) Genetically enhanced maize plants
WO2019150200A2 (en) Dna free crispr plant transformation
WO2025034520A2 (en) Targeted recombination
US11859219B1 (en) Methods of altering a target nucleotide sequence with an RNA-guided nuclease and a single guide RNA
EP3999103A1 (en) Improved homology dependent repair genome editing

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination