JPWO2020045472A5 - - Google Patents
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- Publication number
- JPWO2020045472A5 JPWO2020045472A5 JP2019559121A JP2019559121A JPWO2020045472A5 JP WO2020045472 A5 JPWO2020045472 A5 JP WO2020045472A5 JP 2019559121 A JP2019559121 A JP 2019559121A JP 2019559121 A JP2019559121 A JP 2019559121A JP WO2020045472 A5 JPWO2020045472 A5 JP WO2020045472A5
- Authority
- JP
- Japan
- Prior art keywords
- mutation
- amino acid
- acid sequence
- seq
- sequence represented
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 229920001184 polypeptide Polymers 0.000 claims description 31
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 31
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 31
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 26
- 108090000623 proteins and genes Proteins 0.000 claims description 13
- 125000000539 amino acid group Chemical group 0.000 claims description 9
- 102000004169 proteins and genes Human genes 0.000 claims description 9
- 241000499912 Trichoderma reesei Species 0.000 claims description 8
- 108010059892 Cellulase Proteins 0.000 claims description 7
- 229940106157 cellulase Drugs 0.000 claims description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 230000004568 DNA-binding Effects 0.000 claims description 4
- 108010066805 F-Box Proteins Proteins 0.000 claims description 4
- 102000018700 F-Box Proteins Human genes 0.000 claims description 4
- 239000001913 cellulose Substances 0.000 claims description 2
- 229920002678 cellulose Polymers 0.000 claims description 2
- 230000035772 mutation Effects 0.000 claims 15
- 238000004519 manufacturing process Methods 0.000 claims 8
- 231100000221 frame shift mutation induction Toxicity 0.000 claims 6
- 230000037433 frameshift Effects 0.000 claims 6
- 238000012258 culturing Methods 0.000 claims 5
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims 4
- 239000008101 lactose Substances 0.000 claims 4
- 235000018102 proteins Nutrition 0.000 claims 3
- 239000002028 Biomass Substances 0.000 claims 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims 1
- 235000004279 alanine Nutrition 0.000 claims 1
- 125000003295 alanine group Chemical group N[C@@H](C)C(=O)* 0.000 claims 1
- 230000037430 deletion Effects 0.000 claims 1
- 238000012217 deletion Methods 0.000 claims 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims 1
- 125000000404 glutamine group Chemical group N[C@@H](CCC(N)=O)C(=O)* 0.000 claims 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims 1
- 125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 claims 1
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 6
- 241000024277 Trichoderma reesei QM6a Species 0.000 description 5
- 206010064571 Gene mutation Diseases 0.000 description 4
- POSZUTFLHGNLHX-KSBRXOFISA-N tris maleate Chemical compound OCC(N)(CO)CO.OCC(N)(CO)CO.OC(=O)\C=C/C(O)=O POSZUTFLHGNLHX-KSBRXOFISA-N 0.000 description 4
- 230000027455 binding Effects 0.000 description 3
- 239000002773 nucleotide Substances 0.000 description 3
- 125000003729 nucleotide group Chemical group 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 238000000105 evaporative light scattering detection Methods 0.000 description 2
- 230000014509 gene expression Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000004704 ultra performance liquid chromatography Methods 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- -1 BEH Amide Chemical class 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 102000012858 Eukaryotic Initiation Factor-4G Human genes 0.000 description 1
- 108010057192 Eukaryotic Initiation Factor-4G Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 102000002812 Heat-Shock Proteins Human genes 0.000 description 1
- 108010004889 Heat-Shock Proteins Proteins 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 230000022131 cell cycle Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 229940059442 hemicellulase Drugs 0.000 description 1
- 108010002430 hemicellulase Proteins 0.000 description 1
- 238000002013 hydrophilic interaction chromatography Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2018160156 | 2018-08-29 | ||
| JP2018160156 | 2018-08-29 | ||
| PCT/JP2019/033642 WO2020045472A1 (ja) | 2018-08-29 | 2019-08-28 | トリコデルマ・リーセイの変異株およびそれを用いたタンパク質の製造方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JPWO2020045472A1 JPWO2020045472A1 (ja) | 2021-08-10 |
| JPWO2020045472A5 true JPWO2020045472A5 (https=) | 2022-08-09 |
| JP7388194B2 JP7388194B2 (ja) | 2023-11-29 |
Family
ID=69644318
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019559121A Active JP7388194B2 (ja) | 2018-08-29 | 2019-08-28 | トリコデルマ・リーセイの変異株およびそれを用いたタンパク質の製造方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US12358958B2 (https=) |
| EP (1) | EP3845630A4 (https=) |
| JP (1) | JP7388194B2 (https=) |
| CN (1) | CN112639073A (https=) |
| AU (1) | AU2019329357B2 (https=) |
| BR (1) | BR112021003459A2 (https=) |
| CA (1) | CA3111168A1 (https=) |
| WO (1) | WO2020045472A1 (https=) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7334620B2 (ja) | 2018-03-26 | 2023-08-29 | 東レ株式会社 | トリコデルマ・リーセイ変異株およびタンパク質の製造方法 |
| US20250179459A1 (en) | 2022-03-08 | 2025-06-05 | Toray Industries, Inc. | Filter aid, method of filtration treatment, and method of manufacturing cellulase |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008027472A2 (en) * | 2006-08-29 | 2008-03-06 | Danisco Us, Inc., Genencor Division | Compositions and methods for improved protein production |
| CN102311951B (zh) * | 2011-07-13 | 2013-03-20 | 深圳大学 | 里氏木霉组成型表达盒、表达载体、重组菌株及其应用 |
| RU2645252C2 (ru) | 2012-01-05 | 2018-02-19 | Глюкос Финланд Ой | Клетка нитчатых грибов с дефицитом протеаз и способы ее применения |
| JP2014150745A (ja) * | 2013-02-06 | 2014-08-25 | Nagaoka Univ Of Technology | トリコデルマ属に属する微生物の変異株および該変異株の使用 |
| CN103614303B (zh) * | 2013-11-26 | 2015-12-02 | 青岛蔚蓝生物集团有限公司 | 一种表达糖化酶的里氏木霉菌株 |
| FR3018522B1 (fr) * | 2014-03-17 | 2018-02-02 | IFP Energies Nouvelles | Souches mutantes de trichoderma reesei |
| US10590497B2 (en) * | 2016-03-31 | 2020-03-17 | Toray Industries, Inc. | Trichoderma fungus having mutant-type BXL1 gene and method of producing xylooligosaccharide and glucose by using same |
-
2019
- 2019-08-28 BR BR112021003459-5A patent/BR112021003459A2/pt unknown
- 2019-08-28 CA CA3111168A patent/CA3111168A1/en active Pending
- 2019-08-28 CN CN201980055327.XA patent/CN112639073A/zh active Pending
- 2019-08-28 EP EP19854125.2A patent/EP3845630A4/en active Pending
- 2019-08-28 WO PCT/JP2019/033642 patent/WO2020045472A1/ja not_active Ceased
- 2019-08-28 US US17/271,940 patent/US12358958B2/en active Active
- 2019-08-28 AU AU2019329357A patent/AU2019329357B2/en active Active
- 2019-08-28 JP JP2019559121A patent/JP7388194B2/ja active Active
-
2025
- 2025-06-11 US US19/234,898 patent/US20250304631A1/en active Pending
- 2025-06-11 US US19/235,116 patent/US20250304632A1/en active Pending
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