JP7388194B2 - トリコデルマ・リーセイの変異株およびそれを用いたタンパク質の製造方法 - Google Patents

トリコデルマ・リーセイの変異株およびそれを用いたタンパク質の製造方法 Download PDF

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JP7388194B2
JP7388194B2 JP2019559121A JP2019559121A JP7388194B2 JP 7388194 B2 JP7388194 B2 JP 7388194B2 JP 2019559121 A JP2019559121 A JP 2019559121A JP 2019559121 A JP2019559121 A JP 2019559121A JP 7388194 B2 JP7388194 B2 JP 7388194B2
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mutation
amino acid
seq
mutant strain
sequence represented
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JPWO2020045472A1 (ja
JPWO2020045472A5 (https=
Inventor
雄介 加川
悠香 齋藤
紳吾 平松
勝成 山田
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Toray Industries Inc
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Toray Industries Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/885—Trichoderma

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2019559121A 2018-08-29 2019-08-28 トリコデルマ・リーセイの変異株およびそれを用いたタンパク質の製造方法 Active JP7388194B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2018160156 2018-08-29
JP2018160156 2018-08-29
PCT/JP2019/033642 WO2020045472A1 (ja) 2018-08-29 2019-08-28 トリコデルマ・リーセイの変異株およびそれを用いたタンパク質の製造方法

Publications (3)

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JPWO2020045472A1 JPWO2020045472A1 (ja) 2021-08-10
JPWO2020045472A5 JPWO2020045472A5 (https=) 2022-08-09
JP7388194B2 true JP7388194B2 (ja) 2023-11-29

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JP2019559121A Active JP7388194B2 (ja) 2018-08-29 2019-08-28 トリコデルマ・リーセイの変異株およびそれを用いたタンパク質の製造方法

Country Status (8)

Country Link
US (3) US12358958B2 (https=)
EP (1) EP3845630A4 (https=)
JP (1) JP7388194B2 (https=)
CN (1) CN112639073A (https=)
AU (1) AU2019329357B2 (https=)
BR (1) BR112021003459A2 (https=)
CA (1) CA3111168A1 (https=)
WO (1) WO2020045472A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7334620B2 (ja) 2018-03-26 2023-08-29 東レ株式会社 トリコデルマ・リーセイ変異株およびタンパク質の製造方法
US20250179459A1 (en) 2022-03-08 2025-06-05 Toray Industries, Inc. Filter aid, method of filtration treatment, and method of manufacturing cellulase

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015512611A (ja) 2012-01-05 2015-04-30 ノバルティス アーゲー プロテアーゼ欠損糸状菌細胞及びその使用方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008027472A2 (en) * 2006-08-29 2008-03-06 Danisco Us, Inc., Genencor Division Compositions and methods for improved protein production
CN102311951B (zh) * 2011-07-13 2013-03-20 深圳大学 里氏木霉组成型表达盒、表达载体、重组菌株及其应用
JP2014150745A (ja) * 2013-02-06 2014-08-25 Nagaoka Univ Of Technology トリコデルマ属に属する微生物の変異株および該変異株の使用
CN103614303B (zh) * 2013-11-26 2015-12-02 青岛蔚蓝生物集团有限公司 一种表达糖化酶的里氏木霉菌株
FR3018522B1 (fr) * 2014-03-17 2018-02-02 IFP Energies Nouvelles Souches mutantes de trichoderma reesei
US10590497B2 (en) * 2016-03-31 2020-03-17 Toray Industries, Inc. Trichoderma fungus having mutant-type BXL1 gene and method of producing xylooligosaccharide and glucose by using same

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015512611A (ja) 2012-01-05 2015-04-30 ノバルティス アーゲー プロテアーゼ欠損糸状菌細胞及びその使用方法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ACCESSION No. EGR45828，predicted protein, partial [Trichoderma reesei QM6a]，GenBank[online]，[retrieved on 2019.11.14]，2016年07月25日，Retrieved from the Internet:https://www.ncbi.nlm.nih.gov/protein/EGR45828.1/
ACCESSION No. EGR47155，predicted protein [Trichoderma reesei QM6a]，GenBank[online]，[retrieved on 2019.11.14]，2016年07月25日，Retrieved from the Internet:https://www.ncbi.nlm.nih.gov/protein/EGR47155
ACCESSION No. ETS02047，hypothetical protein M419DRAFT_98837 [Trichoderma reesei RUT C-30]，GenBank[online]，[retrieved on 2019.11.14]，2015年03月23日，Retrieved from the Internet:https://www.ncbi.nlm.nih.gov/protein/ETS02047
Biotechnology and Biology of Trichoderma，2014年，pp.89-102

Also Published As

Publication number Publication date
EP3845630A1 (en) 2021-07-07
BR112021003459A2 (pt) 2021-05-11
JPWO2020045472A1 (ja) 2021-08-10
US20210324018A1 (en) 2021-10-21
US12358958B2 (en) 2025-07-15
CA3111168A1 (en) 2020-03-05
CN112639073A (zh) 2021-04-09
WO2020045472A1 (ja) 2020-03-05
AU2019329357B2 (en) 2025-07-10
US20250304631A1 (en) 2025-10-02
EP3845630A4 (en) 2022-06-15
US20250304632A1 (en) 2025-10-02
AU2019329357A1 (en) 2021-03-18

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