JPWO2013027770A1 - ルシフェラーゼの発光基質 - Google Patents
ルシフェラーゼの発光基質 Download PDFInfo
- Publication number
- JPWO2013027770A1 JPWO2013027770A1 JP2013530040A JP2013530040A JPWO2013027770A1 JP WO2013027770 A1 JPWO2013027770 A1 JP WO2013027770A1 JP 2013530040 A JP2013530040 A JP 2013530040A JP 2013530040 A JP2013530040 A JP 2013530040A JP WO2013027770 A1 JPWO2013027770 A1 JP WO2013027770A1
- Authority
- JP
- Japan
- Prior art keywords
- compound
- luciferin
- present
- firefly
- luciferase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000758 substrate Substances 0.000 title claims abstract description 44
- 108060001084 Luciferase Proteins 0.000 title claims abstract description 36
- 239000005089 Luciferase Substances 0.000 title claims abstract description 29
- 150000001875 compounds Chemical class 0.000 claims description 103
- 229910052799 carbon Inorganic materials 0.000 claims description 35
- 125000000217 alkyl group Chemical group 0.000 claims description 29
- 229910052739 hydrogen Inorganic materials 0.000 claims description 20
- 150000003839 salts Chemical class 0.000 claims description 18
- 238000000504 luminescence detection Methods 0.000 claims description 3
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical class OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 abstract description 62
- 241000254158 Lampyridae Species 0.000 abstract description 27
- 238000004020 luminiscence type Methods 0.000 abstract description 27
- 238000005415 bioluminescence Methods 0.000 abstract description 26
- 230000029918 bioluminescence Effects 0.000 abstract description 26
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 abstract description 18
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 abstract description 18
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 abstract description 18
- 230000000694 effects Effects 0.000 abstract description 13
- IOJUPLGTWVMSFF-UHFFFAOYSA-N benzothiazole Chemical group C1=CC=C2SC=NC2=C1 IOJUPLGTWVMSFF-UHFFFAOYSA-N 0.000 abstract description 6
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- 125000003277 amino group Chemical group 0.000 description 9
- OAYLNYINCPYISS-UHFFFAOYSA-N ethyl acetate;hexane Chemical compound CCCCCC.CCOC(C)=O OAYLNYINCPYISS-UHFFFAOYSA-N 0.000 description 9
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- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 8
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- 238000001727 in vivo Methods 0.000 description 7
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- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 6
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- YHIPILPTUVMWQT-UHFFFAOYSA-N Oplophorus luciferin Chemical compound C1=CC(O)=CC=C1CC(C(N1C=C(N2)C=3C=CC(O)=CC=3)=O)=NC1=C2CC1=CC=CC=C1 YHIPILPTUVMWQT-UHFFFAOYSA-N 0.000 description 6
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- WJKHJLXJJJATHN-UHFFFAOYSA-N triflic anhydride Chemical compound FC(F)(F)S(=O)(=O)OS(=O)(=O)C(F)(F)F WJKHJLXJJJATHN-UHFFFAOYSA-N 0.000 description 6
- GLGNXYJARSMNGJ-VKTIVEEGSA-N (1s,2s,3r,4r)-3-[[5-chloro-2-[(1-ethyl-6-methoxy-2-oxo-4,5-dihydro-3h-1-benzazepin-7-yl)amino]pyrimidin-4-yl]amino]bicyclo[2.2.1]hept-5-ene-2-carboxamide Chemical compound CCN1C(=O)CCCC2=C(OC)C(NC=3N=C(C(=CN=3)Cl)N[C@H]3[C@H]([C@@]4([H])C[C@@]3(C=C4)[H])C(N)=O)=CC=C21 GLGNXYJARSMNGJ-VKTIVEEGSA-N 0.000 description 5
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- 125000003118 aryl group Chemical group 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 235000010233 benzoic acid Nutrition 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000006172 buffering agent Substances 0.000 description 1
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 description 1
- 239000000920 calcium hydroxide Substances 0.000 description 1
- 229910001861 calcium hydroxide Inorganic materials 0.000 description 1
- 150000001718 carbodiimides Chemical class 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 238000003763 carbonization Methods 0.000 description 1
- 239000007810 chemical reaction solvent Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 229930016911 cinnamic acid Natural products 0.000 description 1
- 235000013985 cinnamic acid Nutrition 0.000 description 1
- 235000015165 citric acid Nutrition 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 239000012230 colorless oil Substances 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 229940125773 compound 10 Drugs 0.000 description 1
- 229940125807 compound 37 Drugs 0.000 description 1
- 230000001268 conjugating effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 108010031180 cypridina luciferase Proteins 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 229960002433 cysteine Drugs 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 108010057167 dimethylaniline monooxygenase (N-oxide forming) Proteins 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- AFAXGSQYZLGZPG-UHFFFAOYSA-N ethanedisulfonic acid Chemical compound OS(=O)(=O)CCS(O)(=O)=O AFAXGSQYZLGZPG-UHFFFAOYSA-N 0.000 description 1
- 230000005281 excited state Effects 0.000 description 1
- LIYGYAHYXQDGEP-UHFFFAOYSA-N firefly oxyluciferin Natural products Oc1csc(n1)-c1nc2ccc(O)cc2s1 LIYGYAHYXQDGEP-UHFFFAOYSA-N 0.000 description 1
- OXZOLXJZTSUDOM-UHFFFAOYSA-N fluoro 2,2,2-trifluoroacetate Chemical compound FOC(=O)C(F)(F)F OXZOLXJZTSUDOM-UHFFFAOYSA-N 0.000 description 1
- 239000001530 fumaric acid Substances 0.000 description 1
- 235000011087 fumaric acid Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- PYGSKMBEVAICCR-UHFFFAOYSA-N hexa-1,5-diene Chemical group C=CCCC=C PYGSKMBEVAICCR-UHFFFAOYSA-N 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 1
- IXCSERBJSXMMFS-UHFFFAOYSA-N hydrogen chloride Substances Cl.Cl IXCSERBJSXMMFS-UHFFFAOYSA-N 0.000 description 1
- 229910000041 hydrogen chloride Inorganic materials 0.000 description 1
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 description 1
- 229940071870 hydroiodic acid Drugs 0.000 description 1
- 239000005457 ice water Substances 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000011503 in vivo imaging Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 150000007529 inorganic bases Chemical class 0.000 description 1
- 238000007912 intraperitoneal administration Methods 0.000 description 1
- 238000000752 ionisation method Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- ZLVXBBHTMQJRSX-VMGNSXQWSA-N jdtic Chemical compound C1([C@]2(C)CCN(C[C@@H]2C)C[C@H](C(C)C)NC(=O)[C@@H]2NCC3=CC(O)=CC=C3C2)=CC=CC(O)=C1 ZLVXBBHTMQJRSX-VMGNSXQWSA-N 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000000891 luminescent agent Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- UEGPKNKPLBYCNK-UHFFFAOYSA-L magnesium acetate Chemical compound [Mg+2].CC([O-])=O.CC([O-])=O UEGPKNKPLBYCNK-UHFFFAOYSA-L 0.000 description 1
- 239000011654 magnesium acetate Substances 0.000 description 1
- 235000011285 magnesium acetate Nutrition 0.000 description 1
- 229940069446 magnesium acetate Drugs 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
- 239000011976 maleic acid Substances 0.000 description 1
- 229960002510 mandelic acid Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 229940098779 methanesulfonic acid Drugs 0.000 description 1
- WBYWAXJHAXSJNI-UHFFFAOYSA-N methyl p-hydroxycinnamate Natural products OC(=O)C=CC1=CC=CC=C1 WBYWAXJHAXSJNI-UHFFFAOYSA-N 0.000 description 1
- 150000007522 mineralic acids Chemical class 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000002808 molecular sieve Substances 0.000 description 1
- LNOPIUAQISRISI-UHFFFAOYSA-N n'-hydroxy-2-propan-2-ylsulfonylethanimidamide Chemical compound CC(C)S(=O)(=O)CC(N)=NO LNOPIUAQISRISI-UHFFFAOYSA-N 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- JRZJOMJEPLMPRA-UHFFFAOYSA-N olefin Natural products CCCCCCCC=C JRZJOMJEPLMPRA-UHFFFAOYSA-N 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 125000000962 organic group Chemical group 0.000 description 1
- 235000006408 oxalic acid Nutrition 0.000 description 1
- JJVOROULKOMTKG-UHFFFAOYSA-N oxidized Photinus luciferin Chemical compound S1C2=CC(O)=CC=C2N=C1C1=NC(=O)CS1 JJVOROULKOMTKG-UHFFFAOYSA-N 0.000 description 1
- 238000001139 pH measurement Methods 0.000 description 1
- WLJNZVDCPSBLRP-UHFFFAOYSA-N pamoic acid Chemical compound C1=CC=C2C(CC=3C4=CC=CC=C4C=C(C=3O)C(=O)O)=C(O)C(C(O)=O)=CC2=C1 WLJNZVDCPSBLRP-UHFFFAOYSA-N 0.000 description 1
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- NNFCIKHAZHQZJG-UHFFFAOYSA-N potassium cyanide Chemical compound [K+].N#[C-] NNFCIKHAZHQZJG-UHFFFAOYSA-N 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 239000008057 potassium phosphate buffer Substances 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- LJCNRYVRMXRIQR-OLXYHTOASA-L potassium sodium L-tartrate Chemical compound [Na+].[K+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O LJCNRYVRMXRIQR-OLXYHTOASA-L 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 229940005657 pyrophosphoric acid Drugs 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000007363 ring formation reaction Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 229960004889 salicylic acid Drugs 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- UQDJGEHQDNVPGU-UHFFFAOYSA-N serine phosphoethanolamine Chemical compound [NH3+]CCOP([O-])(=O)OCC([NH3+])C([O-])=O UQDJGEHQDNVPGU-UHFFFAOYSA-N 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 235000011006 sodium potassium tartrate Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- ISIJQEHRDSCQIU-UHFFFAOYSA-N tert-butyl 2,7-diazaspiro[4.5]decane-7-carboxylate Chemical compound C1N(C(=O)OC(C)(C)C)CCCC11CNCC1 ISIJQEHRDSCQIU-UHFFFAOYSA-N 0.000 description 1
- 150000003613 toluenes Chemical class 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- IIHPVYJPDKJYOU-UHFFFAOYSA-N triphenylcarbethoxymethylenephosphorane Chemical compound C=1C=CC=CC=1P(C=1C=CC=CC=1)(=CC(=O)OCC)C1=CC=CC=C1 IIHPVYJPDKJYOU-UHFFFAOYSA-N 0.000 description 1
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 1
- 238000007794 visualization technique Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/001—Preparation for luminescence or biological staining
- A61K49/0013—Luminescence
- A61K49/0017—Fluorescence in vivo
- A61K49/0019—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules
- A61K49/0021—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules the fluorescent group being a small organic molecule
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/001—Preparation for luminescence or biological staining
- A61K49/0013—Luminescence
- A61K49/0017—Fluorescence in vivo
- A61K49/0019—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules
- A61K49/0021—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules the fluorescent group being a small organic molecule
- A61K49/003—Thiazine dyes
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- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D277/00—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings
- C07D277/02—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings
- C07D277/08—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member
- C07D277/12—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
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- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D277/00—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings
- C07D277/60—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings condensed with carbocyclic rings or ring systems
- C07D277/62—Benzothiazoles
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- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D277/00—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings
- C07D277/60—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings condensed with carbocyclic rings or ring systems
- C07D277/62—Benzothiazoles
- C07D277/64—Benzothiazoles with only hydrocarbon or substituted hydrocarbon radicals attached in position 2
- C07D277/66—Benzothiazoles with only hydrocarbon or substituted hydrocarbon radicals attached in position 2 with aromatic rings or ring systems directly attached in position 2
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- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
- C07D417/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings
- C07D417/04—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings directly linked by a ring-member-to-ring-member bond
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- C07D417/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings
- C07D417/06—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
- G01N21/763—Bioluminescence
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Abstract
Description
近年、生物学的事象および現象の可視化が重要視され、可視化のための材料の拡大が望まれてきている。これに伴い、標識技術にも多様化が求められている。特に分子イメージングのための標識技術は、診断および検査機器の進歩と相まって大きく発展している。たとえば、癌や心疾患などに対する個別化医療などの先端技術に応用するための標識技術が精力的に研究されている。また、計測技術の進歩に伴い、より高感度および高性能な機器や標識材料に対する需要が急速に高まっている。
多現象を観測するために、標識を利用した検出系においても、多色発光が求められている。このため、検出系に利用できる標識材料の波長域は、幅広い方が望ましい。たとえば、多色発光を利用した研究には、標識として450nm程度〜650nm以上の波長、特に680nm以上において発光を有する標識材料が準備されることが望ましい。また、生体内深部標識における用途では、短波長光よりも長波長光のほうが優れた光透過性を有するという観点から、赤色発光標識材料が望まれている。特に、生体組織の光計測には、波長が650〜900nmの近赤外光が使用されている。可視光(400〜700nm)は、ヘモグロビンやそのほかの生体構成物質の吸収が大きく、また近赤外光よりも長い波長では、水の吸収が大きくなるため、生体内を光が進むことができない。これに対して近赤外線の波長の領域は生体を透過しやすいため、「生体の窓」とも呼ばれている。
1.プロメガ株式会社:Chroma-Luc:約613nm(非特許文献1)
この系は、ヒカリコメツキ虫(クリックビートル)の突然変異体および天然型ホタル発光基質を利用した系である。
2.東洋紡績株式会社:MultiReporter Assay System-Tripluc:約630nm(非特許文献2)
この系は、鉄道虫赤色発光酵素および天然型ホタル発光基質を利用した系である。緑色発光ルシフェラーゼ(SLG、最大発光波長550nm)、橙色発光ルシフェラーゼ(SLO、580nm)および赤色発光ルシフェラーゼ(SLR、630nm)の色のルシフェラーゼ遺伝子を使用して発光色を変化させている。これは、異なる発光色を与える発光酵素を利用している。
3.東京大学:アミノルシフェリン:約610nm(特許文献1)
これは、ルシフェリン誘導体を開示している。
4.プロメガ株式会社:Chroma-Luc: 約480nm(非特許文献3)
この系は、セレンテラジンおよびウミシイタケルシフェラーゼを利用した系である。
5.アトー株式会社:ウミホタル生物発光 約460nm(非特許文献4)
セレンテラジン系基質およびウミホタルルシフェラーゼを利用した系である。
さらに、本発明者らは、特許文献4において、ルシフェリンとは異なる骨格を有するルシフェリン類似化合物が、様々な発光波長を有することを開示している。これらのルシフェリン類似化合物においても、さらに発光波長を長波長側にシフトさせることが望まれる。
Zは、
R1は、HまたはC1-4アルキルであり、
R4は、OHまたはNR2R3であり、式中R2およびR3は、HまたはC1-4アルキルであり、
nは、0、1、2または3である。
Zは、
R1は、HまたはC1-4アルキルであり、
R4は、OHまたはNR2R3であり、式中R2およびR3は、HまたはC1-4アルキルであり、
nは、0、1、2または3である。特に、上記化合物において、R1、R2およびR3は、Hであり、R4は、OHであることができる。
R1は、HまたはC1-4アルキルであり、
XおよびYは、それぞれ独立してC、N、SまたはOである。
R1は、HまたはC1-4アルキルであり、
XおよびYは、それぞれ独立してC、N、SまたはOであり、
nは、0、1、2または3である。
R1は、それぞれ独立してHまたはC1-4アルキルであり、
XおよびYは、それぞれ独立してC、N、SまたはOであり、
nは、0、1、2または3である。
pH測定:東洋濾紙株式会社製pH試験紙UNIVを使用して測定した。また、pHメータとして、堀場社製pH/ION METER F-23を使用して測定した。
分析用薄層クロマトグラフィー(TLC):E. Merck社製のTLCプレート、シリカゲル60F254(Art.5715)厚さ0.25mmを使用した。TLC上の化合物の検出はUV照射(254nmまたは365nm)および発色剤に浸した後に加熱して発色させることによって行った。発色剤としてはp-アニスアルデヒド(9.3ml)と酢酸(3.8ml)をエタノール(340ml)に溶解し、濃硫酸(12.5ml)を添加したものを使用した。
反応溶液の冷却は、冷媒を満たしたジュワー瓶に反応容器を浸して行った。室温〜4℃では、氷水、4〜-90℃では、液体窒素−アセトンを冷媒として用いた。反応後の抽出溶液の乾燥は、飽和食塩水にて洗浄後、無水硫酸ナトリウムまたは無水硫酸マグネシウムを加えることで行った。反応後の中和を樹脂で行ったものについては、オルガノ株式会社製陽イオン交換樹脂アンバーライトIR120B NAまたは陰イオン交換樹脂アンバーライトIRA400 OH AGを使用した。溶液の減圧濃縮は、アスピレーターの減圧下(20〜30mmHg)、ロータリーエバポレーターを使用して行った。痕跡量の溶媒の除去は、液体窒素浴で冷却したトラップを装着させた真空ポンプ(約1mmHg)を使用して行った。溶媒の混合比は全て体積比で表した。
蒸留水は、アドバンテック東洋株式会社製GS-200型蒸留水製造装置を使用して蒸留およびイオン交換処理したものを使用した。
7-アリル-ホタルルシフェリンは、以下の合成スキームに従って合成した。
以下の化合物
TBS保護体32の合成
1H NMR(270MHz, CDCl3)δ 0.01(6H, s), 0.74(9H, s), 6.80-7.80(6H, complex)。
1H NMR(270MHz, CDCl3)δ 0.28(6H, s), 1.03(9H, s), 7.00-7.80(6H, complex), 10.10(1H, s)。
1H NMR(270MHz, CDCl3)δ 0.25(6H, s), 1.00(9H, s), 1.43(3H, t, J=7.0Hz), 4.23(2H, q, J=7.1Hz), 5.95(1H, d, J=12.5Hz), 6.47(1H, d, J=16.1Hz), 7.01-7.80(7H, complex)。
1H NMR(270MHz, CD3OD)δ 6.47(1H, d, J=15.8Hz), 7.00-7.90(7H, complex)。
IR(neat) 3380, 3320, 1740, 1600cm-1
1H NMR(270MHz, CDCl3)δ2.47(1H, dd, J=8.1, 13Hz), 2.60(1H, dd, J=5.1, 13Hz), 3.21(1H, dd, J=5.1, 8.1Hz), 3.66(3H, s), 7.18-7.32(9H, complex), 7.40-7.54(6H, complex)
13C NMR(67.8MHz, CD3OD)δ36.90(t), 52.16(q), 53.78(d), 66.83(s), 126.8(d)×3, 127.9(d)×6, 129.6(d)×6, 144.5(s)×3, 174.2(s)。
1H NMR(270MHz, CDCl3)δ 2.75(2H, dd, J=4.6, 7.9Hz), δ3.75(3H, s), 4.77(1H, dd, 2.7, 7.9Hz), 6.35(1H, d, J=16.1Hz), 6.90-7.80(22H, complex)。
1H NMR(270MHz, CD3OD)δ3.63(2H, dd, J=3.1, 8.9Hz), 3.8 1(3H, s), 5.27(1H, t, J=8.9Hz), 7.07-7.13(2H, complex), 7.33(1H, d, J=16.1Hz), 7.55-7.83(4H, complex)。
200μLポリスチレンチューブ内で、リン酸カリウム緩衝液(0.5 M、pH8.0、20μl)基質溶液(2.5mM、20μl)、酵素溶液(20μl)、次いでATP-Mg溶液(10mM、40μl)を混合して発光スペクトル測定を行った。酵素溶液の濃度は、17μMのものを使用した。ただし、ホタルルシフェリンは、1.7μM、フェノール型ルシフェリンは、170μMの酵素をそれぞれ使用した。また、発光スペクトル測定の露光時間は、60秒とした。ただし、ホタルルシフェリンは、5秒で行った。
上記手順を使用して、ホタルルシフェリンおよびナフトール-モノエン型のルシフェリン類似体(上記化合物39)、並びに図1および図2に示したアリル基で修飾された化合物の発光波長を測定した。ホタルルシフェリンは、565nmの発光波長を有していた。また、ナフトール-モノエン型のルシフェリン類似体(上記化合物39)は、660nmの発光波長を有していた。図1および図2に示した化合物は、それぞれ605nmおよび690nmの発光波長を有していた。ホタルルシフェリンのベンゾチアゾール環部分の7位をアリル基で修飾することにより、発光波長が長波長側におよそ30〜40nmシフトした。また、ナフトール-モノエン型のルシフェリン類似体のナフトール部分の5位をアリル基で修飾することにより、発光波長が長波長側におよそ30〜40nmシフトした。
ルシフェラーゼが導入されたトランスジェニック細胞、組織および生物に対して本発明のルシフェリン類似体を投与することにより、発光を生じさせことができる。この反応を利用して、生体の窓である690nm付近の発光波長を有する本発明の化合物を使用することにより、インビボにおける発光を検出しやすくなる。たとえば、移植組織や移植細胞にへルシフェラーゼを導入した後、移植動物に対して本発明の化合物を投与すことにより、移植された生体内における、特に現在は難しいとされる生体内深部の移植組織の状態をインビボにおいて非侵襲的にイメージングすることができる。
の結果、ルシフェリンおよびルシフェリン類似体の構造において、特定の位置に修飾を有することにより、発光波長が長波長側にシフトすることを見いだした。
[0015]
本発明は、以下の一般式Iを有する化合物またはその塩を提供する:
[化1]
式中、
Zは、
[化2]
であり、
R1は、HまたはC1−4アルキルであり、
R4は、OHまたはNR2R3であり、式中R2およびR3は、HまたはC1−4アルキルであり、
nは、0、1、2または3である。
[0016]
また、本発明は、R1、R2およびR3は、Hであり、R4は、OHである、上記化合物またはその塩を提供する。
[0017]
さらに、本発明は、上記記載の化合物を含む、ルシフェラーゼの発光基質を提供する。
[0018]
さらに、本発明は、記載の化合物を含む、発光検出キットを提供する。
発明の効果
[0019]
本発明により、発光活性を損なわずに修飾されたルシフェリンおよびルシフェリン類似体が提供される。特に、本発明により、ホタル生物発光系にお
ける発光波長が、従来の発光基質と比較して、より長波長側へシフトした新規発光基質が提供される。
図面の簡単な説明
[0020]
[図1]本発明の修飾されたホタルルシフェリンの発光波長を示す図。
[図2]本発明の修飾されたホタルルシフェリン類似体の発光波長を示す図。
発明を実施するための形態
[0021]
本発明は、発光活性を有するルシフェリン類似体が提供される。本明細書において、ルシフェリン類似体とは、ルシフェラーゼまたはルシフェラーゼ改変体と反応することによって発光を生じる物質をいう。ルシフェラーゼ改変体は、ルシフェラーゼの遺伝子を改変等することによって、その基質特性およびその発光波長が変化されたルシフェラーゼタンパク質をいう。
[0022]
本発明は、以下の一般式を有する化合物またはその塩を提供する:
[化3]
式中、
Zは、
[化4]
であり、
R1は、HまたはC1−4アルキルであり、
R4は、OHまたはNR2R3であり、式中R2およびR3は、HまたはC1−4アルキルであり、
nは、0、1、2または3である。特に、上記化合物において、R1、R2およびR3は
式中、
R1は、HまたはC1−4アルキルであり、
XおよびYは、それぞれ独立してC、N、SまたはOである。
[0028]
本明細書において、「C1−4アルキル」という用語は、1〜4炭素原子を含む飽和直鎖状または分枝鎖アルキル基、たとえばメチル、エチル、n−プロピル、イソ−プロピル、n−ブチル、イソ−ブチル、sec−ブチルおよびtert−ブチルをいう。同様に、「C1−C3アルキル」という用語は、1〜3炭素原子を含む飽和直鎖状または分枝鎖アルキル基(たとえば、メチル、エチルまたはイソ−プロピル)をいう。
[0029]
上記のように、R1がC1−4アルキルであってもよいことは、当業者であれば容易に想到することができるであろう。たとえば、本発明者らによる上記特許文献2には、本発明の化合物のR1部分に対応する部分がAMPであるルシフェリン類似化合物が、ホタル生物発光系の基質となり得る結果が示されている。したがって、置換としてのこのような低級アルキルは、活性に影響を及ぼす可能性が低いと考えられる。
[0030]
上記一般式Iにおいて、XおよびYは、それぞれ独立してC、N、SまたはOであることができる。XおよびYのヘテロ原子がC、N、SまたはOであってもよいことは、当業者であれば容易に想到することができるであろう。たとえば、本発明者らによる上記特許文献2に記載された種々のルシフェリン類似化合物には、本発明の化合物と対応する部分が種々のヘテロ原子であるルシフェリン類似化合物が、ホタル生物発光系の基質となり得る結果が示されている。
[0031]
一つの態様において、本発明は、一般式IにおいてR1は、Hであり、Xは、Nであり、Yは、Sである、以下の化合物を提供する:
[0032]
[化8]
Claims (4)
- 以下の一般式を有する化合物またはその塩:
Zは、
R1は、HまたはC1-4アルキルであり、
R4は、OHまたはNR2R3であり、式中R2およびR3は、HまたはC1-4アルキルであり、
nは、0、1、2または3である。 - R1、R2およびR3は、Hであり、R4は、OHである、請求項1に記載の化合物またはその塩。
- 請求項1または2に記載の化合物を含む、ルシフェラーゼの発光基質。
- 請求項1または2に記載の化合物を含む、発光検出キット。
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