JPWO2011062147A1 - 歯髄細胞から象牙芽細胞への分化誘導方法 - Google Patents
歯髄細胞から象牙芽細胞への分化誘導方法 Download PDFInfo
- Publication number
- JPWO2011062147A1 JPWO2011062147A1 JP2011541917A JP2011541917A JPWO2011062147A1 JP WO2011062147 A1 JPWO2011062147 A1 JP WO2011062147A1 JP 2011541917 A JP2011541917 A JP 2011541917A JP 2011541917 A JP2011541917 A JP 2011541917A JP WO2011062147 A1 JPWO2011062147 A1 JP WO2011062147A1
- Authority
- JP
- Japan
- Prior art keywords
- differentiation
- pulp
- odontoblasts
- wnt
- signaling pathway
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/14—Alkali metal chlorides; Alkaline earth metal chlorides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/40—Peroxides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/1703—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- A61K38/1709—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/40—Regulators of development
- C12N2501/415—Wnt; Frizzeled
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1305—Adipocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1311—Osteocytes, osteoblasts, odontoblasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1317—Chondrocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1323—Adult fibroblasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1329—Cardiomyocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1335—Skeletal muscle cells, myocytes, myoblasts, myotubes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1341—Tenocytes, cells from tendons and ligaments
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1347—Smooth muscle cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1352—Mesenchymal stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1352—Mesenchymal stem cells
- C12N2502/1358—Bone marrow mesenchymal stem cells (BM-MSC)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1352—Mesenchymal stem cells
- C12N2502/1364—Dental pulp stem cells, dental follicle stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1352—Mesenchymal stem cells
- C12N2502/137—Blood-borne mesenchymal stem cells, e.g. Msc from umbilical cord blood
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1352—Mesenchymal stem cells
- C12N2502/1376—Mesenchymal stem cells from hair follicles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1352—Mesenchymal stem cells
- C12N2502/1382—Adipose-derived stem cells [ADSC], adipose stromal stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1352—Mesenchymal stem cells
- C12N2502/1388—Mesenchymal stem cells from other natural sources
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1394—Bone marrow stromal cells; whole marrow
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0664—Dental pulp stem cells, Dental follicle stem cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Inorganic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Gastroenterology & Hepatology (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Alternative & Traditional Medicine (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Mycology (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Marine Sciences & Fisheries (AREA)
- Rheumatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
1.Wntシグナル伝達経路を活性化しうる物質を用いることを特徴とする、歯髄細胞から象牙芽細胞への分化誘導方法。
2.Wntシグナル伝達経路を活性化しうる物質が、直接的又は間接的にWntシグナル伝達経路を活性化しうる物質である、前項1に記載の分化誘導方法。
3.間接的にWntシグナル伝達経路を活性化しうる物質が、ヘパラン硫酸プロテオグリカンとWntの相互作用を調節しうる物質である前項2に記載の分化誘導方法。
4.ヘパラン硫酸プロテオグリカンとWntの相互作用を調節しうる物質が、ヘパラン硫酸プロテオグリカンから硫酸基を除去しうる物質である前項3に記載の分化誘導方法。
5.Wntシグナル伝達経路を活性化しうる物質が、塩素酸ナトリウム、過塩素酸ナトリウム、塩化リチウム、Norrin及びR-Spondin2から選択されるいずれかである前項1〜4のいずれか1に記載の分化誘導方法。
6.Wntが、Wnt10aである前項1〜5のいずれか1に記載の分化誘導方法。
7.Wntシグナル伝達経路を活性化しうる物質からなる、歯髄細胞から象牙芽細胞への分化誘導剤。
8.Wntシグナル伝達経路を活性化しうる物質が、塩素酸ナトリウム、過塩素酸ナトリウム、塩化リチウム、Norrin及びR-Spondin2から選択されるいずれかである前項7に記載の分化誘導剤。
9.前項7又は8に記載の分化誘導剤を有効成分とする覆髄材。
10.さらにフィブロネクチンを含む、前項9に記載の覆髄材。
本実施例では、歯髄由来細胞株であるMEDP細胞を、NaClO3を含む系で培養したときの細胞におけるDsppの発現を確認した。Dsppの発現は、PCRにより確認した。
(配列番号1)forward 5'-AGCCGTGGAGATGCTTCTTA-3'
(配列番号2)reverse 5'-TCACTCTGGCTGTCACCATC-3'
B.GADPH増幅用プライマー
(配列番号3)forward 5'-TGCACCACCAACTGCTTAG-3'
(配列番号4)reverse 5'-GGATGCAGGGATGATGTTC-3'
本実施例では、歯髄由来細胞株であるMEDP細胞を、LiClを含む系で培養したときの細胞におけるDsppの発現を確認した。Dsppの発現は、PCRにより確認した。
NaClO3の代わりにLiClを用い、培養1日目に、最終濃度が5 mM又は50 mMとなるようにLiClを添加した以外は、実施例1と同手法により分化誘導処理を行い、Dsppの発現を確認した。
本実施例では、歯髄由来細胞株であるMEDP細胞を、NaClO4を含む系で培養したときの細胞におけるDsppの発現を確認した。Dsppの発現は、PCRにより確認した。
本実施例では、歯髄由来細胞株であるMEDP細胞を、Norrinを含む系で培養したときの細胞におけるDsppの発現を確認する。Dsppの発現は、PCRにより確認する。
NaClO3の代わりにNorrin(R&D systems社:catalog nNo.:3014-NR)を用いる以外は、実施例1と同手法により分化誘導処理を行い、Dsppの発現を確認する。Norrinの添加濃度については検討する。
本実施例では、歯髄由来細胞株であるMEDP細胞を、R-Spondin2を含む系で培養したときの細胞におけるDsppの発現を確認する。Dsppの発現は、PCRにより確認する。
NaClO3の代わりにR-Spondin2(R&D systems社:catalog nNo.:3266-RS/CF)を用いる以外は、実施例1と同手法により分化誘導処理を行い、Dsppの発現を確認する。R-Spondin2の添加濃度については検討する。
充填することで、う蝕の直下に象牙質が活発に再生されることが期待される。十分な二次(修復)象牙質の形成が確認された後、う蝕部位を削除し、レジンあるいは金属による修復処置が行われる。
Claims (10)
- Wntシグナル伝達経路を活性化しうる物質を用いることを特徴とする、歯髄細胞から象牙芽細胞への分化誘導方法。
- Wntシグナル伝達経路を活性化しうる物質が、直接的又は間接的にWntシグナル伝達経路を活性化しうる物質である、請求項1に記載の分化誘導方法。
- 間接的にWntシグナル伝達経路を活性化しうる物質が、ヘパラン硫酸プロテオグリカンとWntの相互作用を調節しうる物質である請求項2に記載の分化誘導方法。
- ヘパラン硫酸プロテオグリカンとWntの相互作用を調節しうる物質が、ヘパラン硫酸プロテオグリカンから硫酸基を除去しうる物質である請求項3に記載の分化誘導方法。
- Wntシグナル伝達経路を活性化しうる物質が、塩素酸ナトリウム、過塩素酸ナトリウム、塩化リチウム、Norrin及びR-Spondin2から選択されるいずれかである請求項1〜4のいずれか1に記載の分化誘導方法。
- Wntが、Wnt10aである請求項1〜5のいずれか1に記載の分化誘導方法。
- Wntシグナル伝達経路を活性化しうる物質からなる、歯髄細胞から象牙芽細胞への分化誘導剤。
- Wntシグナル伝達経路を活性化しうる物質が、塩素酸ナトリウム、過塩素酸ナトリウム、塩化リチウム、Norrin及びR-Spondin2から選択されるいずれかである請求項7に記載の分化誘導剤。
- 請求項7又は8に記載の分化誘導剤を有効成分とする覆髄材。
- さらにフィブロネクチンを含む、請求項9に記載の覆髄材。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2011541917A JP5808053B2 (ja) | 2009-11-17 | 2010-11-16 | 歯髄細胞から象牙芽細胞への分化誘導方法 |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2009262378 | 2009-11-17 | ||
JP2009262378 | 2009-11-17 | ||
PCT/JP2010/070330 WO2011062147A1 (ja) | 2009-11-17 | 2010-11-16 | 歯髄細胞から象牙芽細胞への分化誘導方法 |
JP2011541917A JP5808053B2 (ja) | 2009-11-17 | 2010-11-16 | 歯髄細胞から象牙芽細胞への分化誘導方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPWO2011062147A1 true JPWO2011062147A1 (ja) | 2013-04-04 |
JP5808053B2 JP5808053B2 (ja) | 2015-11-10 |
Family
ID=44059625
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2011541917A Active JP5808053B2 (ja) | 2009-11-17 | 2010-11-16 | 歯髄細胞から象牙芽細胞への分化誘導方法 |
Country Status (5)
Country | Link |
---|---|
US (1) | US8993000B2 (ja) |
EP (1) | EP2502985B1 (ja) |
JP (1) | JP5808053B2 (ja) |
CN (1) | CN102686720B (ja) |
WO (1) | WO2011062147A1 (ja) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2015009261A2 (en) * | 2013-07-16 | 2015-01-22 | Yeditepe Universitesi | Product used in differentiation of human tooth stem cells |
KR101524488B1 (ko) * | 2013-08-01 | 2015-06-01 | 전남대학교산학협력단 | 상아질 모세포의 분화 유도를 위한 치과용 조성물 |
EP2889373A1 (en) | 2013-12-25 | 2015-07-01 | Yeditepe Universitesi | Dental differentiation product |
WO2016068257A1 (ja) * | 2014-10-31 | 2016-05-06 | 国立大学法人東北大学 | 細胞の製造方法及び造血組織の製造方法 |
JP6887948B2 (ja) * | 2014-12-29 | 2021-06-16 | ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー | 親油性薬剤を歯髄に送達するため、および象牙質生成を増強するための組成物および方法 |
GB201617820D0 (en) * | 2016-10-21 | 2016-12-07 | King's College London | Dental treatment |
EP3967369A4 (en) * | 2019-05-10 | 2022-06-01 | Nippon Shika Yakuhin Co., Ltd. | DENTAL ROOT CANAL FILLING MATERIAL COMPOSITION |
JP2021027803A (ja) * | 2019-08-09 | 2021-02-25 | 国立研究開発法人国立長寿医療研究センター | 象牙質再生用細胞培養物 |
CN114106094B (zh) * | 2021-10-22 | 2023-11-03 | 天津市口腔医院(天津市整形外科医院、南开大学口腔医院) | 一种可诱导人牙髓细胞成牙本质分化和矿化的多肽、多肽衍生物、纳米纤维及其应用 |
WO2024070172A1 (ja) * | 2022-09-26 | 2024-04-04 | 国立大学法人大阪大学 | 歯髄細胞から象牙芽細胞への分化誘導剤及び分化誘導方法 |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH06256132A (ja) | 1993-03-05 | 1994-09-13 | Sunstar Inc | 覆髄剤 |
JPH06340555A (ja) | 1993-06-02 | 1994-12-13 | Suntory Ltd | 象牙質形成覆髄剤 |
JP2002363084A (ja) | 2001-05-31 | 2002-12-18 | Univ Nihon | 第二象牙質形成促進剤 |
JP4125241B2 (ja) * | 2002-02-06 | 2008-07-30 | シュティフトゥング カエザール | 歯に由来する多能性胚性様幹細胞およびその使用 |
JP4592302B2 (ja) | 2003-03-07 | 2010-12-01 | 日本合成化学工業株式会社 | 象牙質再生覆髄剤 |
JP4575687B2 (ja) | 2004-03-18 | 2010-11-04 | リジェンティス株式会社 | 象牙質形成覆髄剤 |
JP2005341961A (ja) | 2004-05-07 | 2005-12-15 | Hitachi Medical Corp | ヒト歯髄細胞の分化誘導方法及び象牙質再生用組成物 |
US7850960B2 (en) * | 2004-12-30 | 2010-12-14 | University Of Washington | Methods for regulation of stem cells |
JP4884678B2 (ja) | 2005-02-03 | 2012-02-29 | 国立大学法人名古屋大学 | ヒト歯髄細胞からの象牙質再生方法 |
DE602006021408D1 (de) * | 2005-04-27 | 2011-06-01 | Univ Leiden Medical Ct | Behandlung von hpv-induzierter intraepithelialer anogenitaler neoplasien |
EP1981910B1 (en) * | 2006-01-13 | 2013-06-26 | A Chan Holding B.V. | Method for identifying inhibitor of the glypican-sclerostin interaction |
WO2008120720A1 (ja) * | 2007-03-30 | 2008-10-09 | National University Corporation Okayama University | 象牙質形成促進剤および象牙質形成覆髄材 |
JP5572291B2 (ja) * | 2008-04-07 | 2014-08-13 | 独立行政法人国立長寿医療研究センター | 歯髄及び/又は象牙質形成促進のための薬剤及びその利用 |
JP2009262378A (ja) | 2008-04-23 | 2009-11-12 | Daikin Ind Ltd | 表面材とその製造方法、建築物、空調機の室外機ケーシング、及び室外熱交換器 |
KR101198918B1 (ko) * | 2009-09-01 | 2012-11-07 | (주)케어젠 | Wnt10?유래 펩타이드 및 그의 용도 |
CN103403148A (zh) * | 2010-10-01 | 2013-11-20 | 纽约市哥伦比亚大学理事会 | 通过细胞产生牙本质、牙骨质和牙釉质 |
KR20140048276A (ko) * | 2011-07-15 | 2014-04-23 | 온코메드 파마슈티칼스, 인크. | Rspo 결합제 및 이의 용도 |
-
2010
- 2010-11-16 JP JP2011541917A patent/JP5808053B2/ja active Active
- 2010-11-16 EP EP20100831541 patent/EP2502985B1/en not_active Not-in-force
- 2010-11-16 WO PCT/JP2010/070330 patent/WO2011062147A1/ja active Application Filing
- 2010-11-16 US US13/510,535 patent/US8993000B2/en active Active
- 2010-11-16 CN CN2010800518716A patent/CN102686720B/zh active Active
Also Published As
Publication number | Publication date |
---|---|
WO2011062147A1 (ja) | 2011-05-26 |
EP2502985B1 (en) | 2015-04-29 |
CN102686720A (zh) | 2012-09-19 |
EP2502985A1 (en) | 2012-09-26 |
US20120231091A1 (en) | 2012-09-13 |
JP5808053B2 (ja) | 2015-11-10 |
EP2502985A4 (en) | 2013-04-24 |
US8993000B2 (en) | 2015-03-31 |
CN102686720B (zh) | 2013-12-04 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP5808053B2 (ja) | 歯髄細胞から象牙芽細胞への分化誘導方法 | |
Zheng et al. | Stem cell-based bone and dental regeneration: a view of microenvironmental modulation | |
US20170258847A1 (en) | Postnatal stem cells and uses thereof | |
Shi et al. | The efficacy of mesenchymal stem cells to regenerate and repair dental structures | |
Prescott et al. | In vivo generation of dental pulp-like tissue by using dental pulp stem cells, a collagen scaffold, and dentin matrix protein 1 after subcutaneous transplantation in mice | |
US11364323B2 (en) | Combination grafts for tissue repair or regeneration applications | |
JP5069572B2 (ja) | 間葉系細胞の製造方法、歯の製造方法及び歯形成用間葉系細胞 | |
Kwack et al. | Clinical potential of dental pulp stem cells in pulp regeneration: current endodontic progress and future perspectives | |
Yeom et al. | Platelet‐rich plasma enhances the differentiation of dental pulp progenitor cells into odontoblasts | |
CN102131489A (zh) | 牙齿缺损部的修复方法及修复材料的制造方法 | |
Jose et al. | Oral tissue regeneration: Current status and future perspectives | |
JPH08501779A (ja) | モルフォゲン誘導歯周組織再生 | |
WO2010021162A1 (ja) | 骨組織再生用の細胞製剤 | |
Bates et al. | Cell-based regenerative approaches to the treatment of oral soft tissue defects. | |
US20110206648A1 (en) | Gene-enhanced tissue engineering | |
Wang et al. | Research progress in cell therapy for oral diseases: Focus on cell sources and strategies to optimize cell function | |
EP1990056A1 (en) | Tooth root formation promoter and method for promotion of tooth root formation | |
JP7297309B2 (ja) | 歯根膜幹細胞が濃縮された細胞集団の製造方法 | |
Hosein | Strategies for whole tooth regeneration and possible rejuvenation of adult dental pulp stem cells. | |
CA2809164C (en) | Postnatal stem cells and uses thereof | |
Koutrouli et al. | Biological responses of two calcium-silicate-based cements on a tissue-engineered 3D organotypic deciduous pulp analogue | |
Rezapour | Comparison of the effects of different pulp capping materials on viability, morphology and ageing of dental pulp cells | |
Gruber | Regulation of the expression of Sclerostin in periodontal fibroblasts | |
Egusa | Gingiva-derived iPS cells: A promising tool for dental applications | |
Galler | Self-assembling peptide hydrogels targeted for dental tissue regeneration |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20150121 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20150316 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20150818 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20150907 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 5808053 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313113 |
|
R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |