JP5808053B2 - 歯髄細胞から象牙芽細胞への分化誘導方法 - Google Patents
歯髄細胞から象牙芽細胞への分化誘導方法 Download PDFInfo
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Description
1.Wntシグナル伝達経路を活性化しうる物質を用いることを特徴とする、歯髄細胞から象牙芽細胞への分化誘導方法。
2.Wntシグナル伝達経路を活性化しうる物質が、直接的又は間接的にWntシグナル伝達経路を活性化しうる物質である、前項1に記載の分化誘導方法。
3.間接的にWntシグナル伝達経路を活性化しうる物質が、ヘパラン硫酸プロテオグリカンとWntの相互作用を調節しうる物質である前項2に記載の分化誘導方法。
4.ヘパラン硫酸プロテオグリカンとWntの相互作用を調節しうる物質が、ヘパラン硫酸プロテオグリカンから硫酸基を除去しうる物質である前項3に記載の分化誘導方法。
5.Wntシグナル伝達経路を活性化しうる物質が、塩素酸ナトリウム、過塩素酸ナトリウム、塩化リチウム、Norrin及びR-Spondin2から選択されるいずれかである前項1〜4のいずれか1に記載の分化誘導方法。
6.Wntが、Wnt10aである前項1〜5のいずれか1に記載の分化誘導方法。
7.Wntシグナル伝達経路を活性化しうる物質からなる、歯髄細胞から象牙芽細胞への分化誘導剤。
8.Wntシグナル伝達経路を活性化しうる物質が、塩素酸ナトリウム、過塩素酸ナトリウム、塩化リチウム、Norrin及びR-Spondin2から選択されるいずれかである前項7に記載の分化誘導剤。
9.前項7又は8に記載の分化誘導剤を有効成分とする覆髄材。
10.さらにフィブロネクチンを含む、前項9に記載の覆髄材。
本実施例では、歯髄由来細胞株であるMEDP細胞を、NaClO3を含む系で培養したときの細胞におけるDsppの発現を確認した。Dsppの発現は、PCRにより確認した。
(配列番号1)forward 5'-AGCCGTGGAGATGCTTCTTA-3'
(配列番号2)reverse 5'-TCACTCTGGCTGTCACCATC-3'
B.GADPH増幅用プライマー
(配列番号3)forward 5'-TGCACCACCAACTGCTTAG-3'
(配列番号4)reverse 5'-GGATGCAGGGATGATGTTC-3'
本実施例では、歯髄由来細胞株であるMEDP細胞を、LiClを含む系で培養したときの細胞におけるDsppの発現を確認した。Dsppの発現は、PCRにより確認した。
NaClO3の代わりにLiClを用い、培養1日目に、最終濃度が5 mM又は50 mMとなるようにLiClを添加した以外は、実施例1と同手法により分化誘導処理を行い、Dsppの発現を確認した。
本実施例では、歯髄由来細胞株であるMEDP細胞を、NaClO4を含む系で培養したときの細胞におけるDsppの発現を確認した。Dsppの発現は、PCRにより確認した。
本実施例では、歯髄由来細胞株であるMEDP細胞を、Norrinを含む系で培養したときの細胞におけるDsppの発現を確認する。Dsppの発現は、PCRにより確認する。
NaClO3の代わりにNorrin(R&D systems社:catalog nNo.:3014-NR)を用いる以外は、実施例1と同手法により分化誘導処理を行い、Dsppの発現を確認する。Norrinの添加濃度については検討する。
本実施例では、歯髄由来細胞株であるMEDP細胞を、R-Spondin2を含む系で培養したときの細胞におけるDsppの発現を確認する。Dsppの発現は、PCRにより確認する。
NaClO3の代わりにR-Spondin2(R&D systems社:catalog nNo.:3266-RS/CF)を用いる以外は、実施例1と同手法により分化誘導処理を行い、Dsppの発現を確認する。R-Spondin2の添加濃度については検討する。
充填することで、う蝕の直下に象牙質が活発に再生されることが期待される。十分な二次(修復)象牙質の形成が確認された後、う蝕部位を削除し、レジンあるいは金属による修復処置が行われる。
Claims (4)
- 塩素酸ナトリウム、及び/又は塩化リチウムを用いることを特徴とする、歯髄細胞から象牙芽細胞への分化誘導方法。
- 塩素酸ナトリウム及び/又は塩化リチウムからなる、歯髄細胞から象牙芽細胞への分化誘導剤。
- 請求項2に記載の分化誘導剤を有効成分とする覆髄材。
- さらにフィブロネクチンを含む、請求項3に記載の覆髄材。
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WO2015009261A2 (en) * | 2013-07-16 | 2015-01-22 | Yeditepe Universitesi | Product used in differentiation of human tooth stem cells |
KR101524488B1 (ko) * | 2013-08-01 | 2015-06-01 | 전남대학교산학협력단 | 상아질 모세포의 분화 유도를 위한 치과용 조성물 |
EP2889373A1 (en) * | 2013-12-25 | 2015-07-01 | Yeditepe Universitesi | Dental differentiation product |
JP6270995B2 (ja) * | 2014-10-31 | 2018-01-31 | 国立大学法人東北大学 | 細胞の製造方法及び造血組織の製造方法 |
EP3240562B1 (en) * | 2014-12-29 | 2023-03-22 | The Board of Trustees of the Leland Stanford Junior University | Compositions and methods for delivering lypophilic agents to dental pulp and for enhancing dentin production |
GB201617820D0 (en) * | 2016-10-21 | 2016-12-07 | King's College London | Dental treatment |
EP3967369A4 (en) * | 2019-05-10 | 2022-06-01 | Nippon Shika Yakuhin Co., Ltd. | DENTAL ROOT CANAL FILLING MATERIAL COMPOSITION |
JP2021027803A (ja) * | 2019-08-09 | 2021-02-25 | 国立研究開発法人国立長寿医療研究センター | 象牙質再生用細胞培養物 |
CN114106094B (zh) * | 2021-10-22 | 2023-11-03 | 天津市口腔医院(天津市整形外科医院、南开大学口腔医院) | 一种可诱导人牙髓细胞成牙本质分化和矿化的多肽、多肽衍生物、纳米纤维及其应用 |
WO2024070172A1 (ja) * | 2022-09-26 | 2024-04-04 | 国立大学法人大阪大学 | 歯髄細胞から象牙芽細胞への分化誘導剤及び分化誘導方法 |
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JPWO2011062147A1 (ja) | 2013-04-04 |
EP2502985B1 (en) | 2015-04-29 |
CN102686720B (zh) | 2013-12-04 |
EP2502985A4 (en) | 2013-04-24 |
CN102686720A (zh) | 2012-09-19 |
US8993000B2 (en) | 2015-03-31 |
US20120231091A1 (en) | 2012-09-13 |
WO2011062147A1 (ja) | 2011-05-26 |
EP2502985A1 (en) | 2012-09-26 |
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