JPWO2008133332A1 - 基質濃度測定方法および基質濃度測定装置 - Google Patents
基質濃度測定方法および基質濃度測定装置 Download PDFInfo
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- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
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- 238000006243 chemical reaction Methods 0.000 description 2
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- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
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- 150000001540 azides Chemical class 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 229920002301 cellulose acetate Polymers 0.000 description 1
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- -1 for example Substances 0.000 description 1
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- 235000019420 glucose oxidase Nutrition 0.000 description 1
- 239000003219 hemolytic agent Substances 0.000 description 1
- 230000002949 hemolytic effect Effects 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 238000005375 photometry Methods 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229920000083 poly(allylamine) Polymers 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
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- 239000011734 sodium Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3274—Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/721—Haemoglobin
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/96—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood or serum control standard
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- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
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Abstract
Description
2 ヘモグロビン測定ユニット(ヘモグロビン測定手段)
3 基質測定ユニット(基質測定手段)
34 酵素電極
5 演算部(演算手段)
その結果、本発明では、血液試料中の基質濃度を精度良く測定することが可能となる。
Claims (12)
- ヘモグロビンを含む血液試料中の基質の濃度を測定する方法であって、
ヘモグロビンにより影響を受けた基質濃度に相関する測定値と、ヘモグロビン濃度またはそれに相関する値と、を用いて基質濃度を演算する、基質濃度測定方法。 - 上記Vmaxは、測定すべき血液試料ごとにヘモグロビン濃度またはそれに相関する値を測定し、その測定結果に基づいて血液試料ごとに決定される、請求項2に記載の基質濃度測定方法。
- 上記Vmaxは、クロマトグラフィにより得られるクロマトグラムに基づいて決定する、請求項3に記載の基質濃度測定方法。
- ヘモグロビンにより影響を受けた基質濃度に相関する測定値Reは、酵素電極法により測定される、請求項2に記載の基質濃度測定方法。
- 測定値Reは、電圧値あるいは電流値である、請求項5に記載の基質濃度測定方法。
- ヘモグロビンを含む血液試料中の基質の濃度を演算するための演算手段と、ヘモグロビンにより影響を受けた基質濃度に相関する値を測定するための基質測定機構と、を備えた基質濃度測定装置であって、
ヘモグロビン濃度またはそれに相関する値を測定するためのヘモグロビン測定機構をさらに備えており、
上記演算手段は、上記基質測定機構における測定値と、上記ヘモグロビン測定機構における測定値と、を用いて基質濃度を演算するように構成されている、基質濃度測定装置。 - 上記演算手段は、上記Vmaxを、測定すべき血液試料ごとにヘモグロビン濃度またはそれに相関する値を測定し、その測定結果に基づいて血液試料ごとに決定するように構成されている、請求項8に記載の基質濃度測定装置。
- 上記ヘモグロビン測定機構は、クロマトグラフィにより得られるクロマトグラムとして上記ヘモグロビン濃度またはそれに相関する値を測定できるように構成されている、請求項9に記載の基質濃度測定装置。
- 上記基質測定機構は、ヘモグロビンにより影響を受けた基質濃度に相関する測定値Reを得るための酵素電極を含んでいる、請求項8に記載の基質濃度測定装置。
- 測定値Reは、上記酵素電極において出力される電流値あるいはその電流値を換算した電圧値である、請求項11に記載の基質濃度測定装置。
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JP2009511920A JP4901956B2 (ja) | 2007-04-25 | 2008-04-25 | 基質濃度測定方法および基質濃度測定装置 |
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JP2007116099 | 2007-04-25 | ||
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JP2009511920A JP4901956B2 (ja) | 2007-04-25 | 2008-04-25 | 基質濃度測定方法および基質濃度測定装置 |
PCT/JP2008/058152 WO2008133332A1 (ja) | 2007-04-25 | 2008-04-25 | 基質濃度測定方法および基質濃度測定装置 |
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JPWO2008133332A1 true JPWO2008133332A1 (ja) | 2010-07-29 |
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US (1) | US20100299072A1 (ja) |
EP (1) | EP2151682B1 (ja) |
JP (1) | JP4901956B2 (ja) |
WO (1) | WO2008133332A1 (ja) |
Families Citing this family (6)
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EP2400034B1 (en) * | 2010-06-23 | 2016-08-24 | ARKRAY, Inc. | Diluent for preparing analytical sample |
JP5888885B2 (ja) * | 2010-06-23 | 2016-03-22 | アークレイ株式会社 | 測定装置、測定方法、測定プログラム、および測定システム |
US9939402B2 (en) * | 2012-02-09 | 2018-04-10 | Lg Electronics Inc. | Blood sugar detecting method and cartridge using same |
EP2972275B1 (en) * | 2013-03-14 | 2019-07-10 | Instrumentation Laboratory Company | Whole blood hemolysis sensor |
US9869654B2 (en) * | 2013-11-29 | 2018-01-16 | Broadmaster Biotech Corp. | Method of measuring hematocrit (HCT), and measurement device using the method |
EP3101416B1 (en) * | 2014-01-27 | 2021-07-21 | Hitachi High-Tech Corporation | Automatic analytical apparatus |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH02159563A (ja) * | 1988-12-12 | 1990-06-19 | Hitachi Ltd | 診断システム |
JP2000346848A (ja) * | 1999-06-04 | 2000-12-15 | Tosoh Corp | グルコースの測定方法及び測定装置 |
WO2005098424A1 (en) * | 2004-03-31 | 2005-10-20 | Bayer Healthcare Llc | Method and apparatus for implementing threshold based correction functions for biosensors |
WO2005103669A1 (ja) * | 2004-04-19 | 2005-11-03 | Matsushita Electric Industrial Co., Ltd. | 血液成分の測定方法、それに用いるバイオセンサおよび測定装置 |
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JP3700140B2 (ja) | 1995-07-21 | 2005-09-28 | アークレイ株式会社 | グルコース濃度の測定方法 |
JP3698281B2 (ja) | 1996-05-29 | 2005-09-21 | アークレイ株式会社 | グルコース濃度の測定方法 |
US7597793B2 (en) * | 2003-06-20 | 2009-10-06 | Roche Operations Ltd. | System and method for analyte measurement employing maximum dosing time delay |
-
2008
- 2008-04-25 JP JP2009511920A patent/JP4901956B2/ja active Active
- 2008-04-25 US US12/451,085 patent/US20100299072A1/en not_active Abandoned
- 2008-04-25 EP EP08740891.0A patent/EP2151682B1/en active Active
- 2008-04-25 WO PCT/JP2008/058152 patent/WO2008133332A1/ja active Application Filing
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH02159563A (ja) * | 1988-12-12 | 1990-06-19 | Hitachi Ltd | 診断システム |
JP2000346848A (ja) * | 1999-06-04 | 2000-12-15 | Tosoh Corp | グルコースの測定方法及び測定装置 |
WO2005098424A1 (en) * | 2004-03-31 | 2005-10-20 | Bayer Healthcare Llc | Method and apparatus for implementing threshold based correction functions for biosensors |
WO2005103669A1 (ja) * | 2004-04-19 | 2005-11-03 | Matsushita Electric Industrial Co., Ltd. | 血液成分の測定方法、それに用いるバイオセンサおよび測定装置 |
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US20100299072A1 (en) | 2010-11-25 |
JP4901956B2 (ja) | 2012-03-21 |
EP2151682A1 (en) | 2010-02-10 |
EP2151682B1 (en) | 2014-09-17 |
WO2008133332A1 (ja) | 2008-11-06 |
EP2151682A4 (en) | 2013-05-15 |
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