JPWO2007086367A1 - Si/Si3N4型ナノ粒子、該ナノ粒子を用いた生体物質標識剤及び前記ナノ粒子の製造方法 - Google Patents
Si/Si3N4型ナノ粒子、該ナノ粒子を用いた生体物質標識剤及び前記ナノ粒子の製造方法 Download PDFInfo
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Abstract
Description
本発明のSi/Si3N4型ナノ粒子について説明する。
本発明のSi/Si3N4型ナノ粒のコアはSiで形成されている。前記Siで形成されるコアの粒径は1nm〜50nmの範囲であるが、好ましくは1nm〜20nmの範囲であり、さらに好ましくは、2nm〜12nmの範囲である。
本発明のSi/Si3N4型ナノ粒のシェルはSi3N4で形成されている。Si3N4はSiと比較してバンドギャップが大きく、またSiO2と比較すると酸素に対する安定性が高いという点で優れており、水分散液中での紫外線照射時の劣化性能が大きく改善されるという長所をもつために好ましい。
また、本発明に係るSi3N4で形成されたシェルは、表面が親水化処理されていてもよく、このような親水化処理された、Si/Si3N4型ナノ粒子は、分子標識物質と有機分子を介して結合させることにより生体物質標識剤として使用できる。
本発明のSi/Si3N4型ナノ粒子の製法方法について説明する。
シェル厚=4:2.5となり、体積比で、モノシランガス:アンモニア=1:1.3の場合、Si/Si3N4型ナノ粒子の粒径に対するシェル厚はおよそ、コアの粒径:シェル厚=2:3.5となる。
本発明のSi/Si3N4型ナノ粒子の構造、物性について説明する。
本発明のSi/Si3N4型ナノ粒子の親水化処理について説明する。
本発明の生体物質標識剤について説明する。
本発明の生体物質標識剤は、上述した親水化処理されたSi/Si3N4型ナノ粒子と、分子標識物質とを有機分子を介して結合させて得られる。
本発明の生体物質標識剤は分子標識物質が目的とする生体物質と特異的に結合および/または反応することにより、生体物質の標識が可能となる。
本発明の生体物質標識剤は、親水化処理されたSi/Si3N4型ナノ粒子と、分子標識物質とが有機分子により結合されている。該有機分子としてはSi/Si3N4型ナノ粒子と分子標識物質とを結合できる有機分子であれば特に制限はないが、例えば、タンパク質中でも、アルブミン、ミオグロビンおよびカゼイン等、またタンパク質の一種であるアビジンをビオチンと共に用いることも好適に用いられる。上記結合の態様としては特に限定されず、共有結合、イオン結合、水素結合、配位結合、物理吸着および化学吸着等が挙げられる。結合の安定性から共有結合などの結合力の強い結合が好ましい。
《Si/Si3N4型ナノ粒子1の製造》
モノシランガス0.70リットル//分、窒素ガス19.00リットル/分、アンモニア0.30リットル/分の混合ガスを温度700℃、圧力50kPaで保持した石英ガラス製の反応管に導入し0.5時間反応させた。このとき反応管の下部に粉末が形成した。
混合ガスをモノシランガス0.48リットル/分、窒素ガス19.00リットル/分、アンモニア0.52リットル/分とし、アニ−ル処理の条件を1時間1100℃とした以外の条件は、実施例1と同様にして表面が親水化処理されたSi/Si3N4型ナノ粒子2を得た。結果を表1に示す。
混合ガスをモノシランガス0.43リットル/分、窒素ガス19.00リットル/分、アンモニア0.57リットル/分とし、アニ−ル処理の条件を0.5時間1100℃とした以外の条件は、実施例1と同様にして表面が親水化処理されたSi/Si3N4型ナノ粒子3を得た。結果を表1に示す。
混合ガスをモノシランガス1.44リットル/分、窒素ガス18.00リットル/分、アンモニア0.56リットル/分とし、反応温度を650℃とし、アニ−ル処理の条件を1時間、1000℃とした以外の条件は、実施例1と同様にして表面が親水化処理されたSi/Si3N4型ナノ粒子4を得た。結果を表1に示す。
混合ガスをモノシランガス0.09リットル/分、窒素ガス19.80リットル/分、アンモニア0.11リットル/分とし、反応温度を850℃とし、アニ−ル処理の条件を1時1250℃とした以外の条件は、実施例1と同様にして表面が親水化処理されたSi/Si3N4型ナノ粒子5を得た。結果を表1に示す。
モノシランガス1.00リットル/分、窒素ガス19.00リットル/分の混合ガスを温度700℃、圧力50kPaで保持した石英ガラス製の反応管に導入し0.5時間反応させた。このとき反応管の下部に粉末が形成した。
測定条件 :グラファイト製試料台を用いて測定を行った。
得られた表面が親水化処理されたSi/Si3N4型ナノ粒子および表面が親水化処理されたSi/SiO2型ナノ粒子の発光特性を以下のような方法で測定した。
《生体物質標識剤の製造》
実施例1の、表面が親水化処理された本発明のSi/Si3N4型ナノ粒子2の水溶液にアビジン25mgを添加し40℃で10分間攪拌を行い、アビジンコンジュゲートSi/Si3N4型ナノ粒子を作製した。
Claims (8)
- Siで形成されたコアとSi3N4で形成されたシェルとを有し、前記コアの粒径が1nm〜50nmの範囲であることを特徴とするSi/Si3N4型ナノ粒子。
- 前記シェルの厚さが1nm〜50nmの範囲であることを特徴とする請求の範囲第1項に記載のSi/Si3N4型ナノ粒子。
- 前記シェルの表面が親水化処理されていることを特徴とする請求の範囲第1項または請求の範囲第2項に記載のSi/Si3N4型ナノ粒子。
- 請求の範囲第3項に記載のSi/Si3N4型ナノ粒子と分子標識物質とを有機分子を介して結合させたことを特徴とする生体物質標識剤。
- 前記分子標識物質がヌクレオチド鎖であることを特徴とする請求の範囲第4項に記載の生体物質標識剤。
- 前記分子標識物質が抗体であることを特徴とする請求の範囲第4項に記載の生体物質標識剤。
- 前記有機分子が、ビオチン及びアビジンであることを特徴とする請求の範囲第4項に記載の生体物質標識剤。
- 請求の範囲第項1または請求の範囲第2項に記載のSi/Si3N4型ナノ粒子を製造するに当たり、モノシランガスとアンモニアとの気相反応を行う工程を有することを特徴とするSi/Si3N4型ナノ粒子の製造方法。
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