JPS62148459A - グルタミンの分離精製法 - Google Patents
グルタミンの分離精製法Info
- Publication number
- JPS62148459A JPS62148459A JP29004485A JP29004485A JPS62148459A JP S62148459 A JPS62148459 A JP S62148459A JP 29004485 A JP29004485 A JP 29004485A JP 29004485 A JP29004485 A JP 29004485A JP S62148459 A JPS62148459 A JP S62148459A
- Authority
- JP
- Japan
- Prior art keywords
- glutamine
- exchange resin
- glutamic acid
- strongly acidic
- cation exchange
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 title claims abstract description 60
- 238000000926 separation method Methods 0.000 title claims abstract description 7
- 238000000746 purification Methods 0.000 title claims abstract description 4
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims abstract description 19
- 239000012535 impurity Substances 0.000 claims abstract description 18
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 230000002378 acidificating effect Effects 0.000 claims abstract description 14
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000003729 cation exchange resin Substances 0.000 claims abstract description 13
- 235000013922 glutamic acid Nutrition 0.000 claims abstract description 13
- 239000004220 glutamic acid Substances 0.000 claims abstract description 13
- 238000001032 ion-exclusion chromatography Methods 0.000 claims abstract description 11
- ODHCTXKNWHHXJC-VKHMYHEASA-N 5-oxo-L-proline Chemical compound OC(=O)[C@@H]1CCC(=O)N1 ODHCTXKNWHHXJC-VKHMYHEASA-N 0.000 claims abstract 3
- 229940079889 pyrrolidonecarboxylic acid Drugs 0.000 claims abstract 3
- 238000000034 method Methods 0.000 claims description 18
- QAOWNCQODCNURD-UHFFFAOYSA-L sulfate group Chemical group S(=O)(=O)([O-])[O-] QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims description 6
- 238000000855 fermentation Methods 0.000 abstract description 19
- 230000004151 fermentation Effects 0.000 abstract description 19
- 239000000243 solution Substances 0.000 abstract description 17
- 239000007788 liquid Substances 0.000 abstract description 16
- 239000007864 aqueous solution Substances 0.000 abstract description 14
- 239000011347 resin Substances 0.000 abstract description 7
- 229920005989 resin Polymers 0.000 abstract description 7
- 239000013078 crystal Substances 0.000 abstract description 5
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 abstract description 4
- 238000002425 crystallisation Methods 0.000 abstract description 4
- 230000008025 crystallization Effects 0.000 abstract description 4
- 239000000049 pigment Substances 0.000 abstract description 4
- 238000004132 cross linking Methods 0.000 abstract description 2
- 239000012452 mother liquor Substances 0.000 abstract 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 61
- 229930182816 L-glutamine Natural products 0.000 description 12
- 229960002989 glutamic acid Drugs 0.000 description 12
- 150000003863 ammonium salts Chemical class 0.000 description 9
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 5
- PHKGGXPMPXXISP-DFWYDOINSA-N azanium;(4s)-4-amino-5-hydroxy-5-oxopentanoate Chemical compound [NH4+].[O-]C(=O)[C@@H]([NH3+])CCC([O-])=O PHKGGXPMPXXISP-DFWYDOINSA-N 0.000 description 5
- 150000001768 cations Chemical class 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 4
- 150000001450 anions Chemical class 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 239000003792 electrolyte Substances 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 239000003957 anion exchange resin Substances 0.000 description 2
- 238000004042 decolorization Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- HCGIOFQTKHOCPM-QTNFYWBSSA-N diazanium;(2s)-2-aminopentanedioate Chemical compound [NH4+].[NH4+].[O-]C(=O)[C@@H](N)CCC([O-])=O HCGIOFQTKHOCPM-QTNFYWBSSA-N 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 239000007785 strong electrolyte Substances 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 229930195714 L-glutamate Natural products 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 229920001429 chelating resin Polymers 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 239000000306 component Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 125000000404 glutamine group Chemical group N[C@@H](CCC(N)=O)C(=O)* 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- VAOCPAMSLUNLGC-UHFFFAOYSA-N metronidazole Chemical compound CC1=NC=C([N+]([O-])=O)N1CCO VAOCPAMSLUNLGC-UHFFFAOYSA-N 0.000 description 1
- 239000001967 plate count agar Chemical class 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP29004485A JPS62148459A (ja) | 1985-12-23 | 1985-12-23 | グルタミンの分離精製法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP29004485A JPS62148459A (ja) | 1985-12-23 | 1985-12-23 | グルタミンの分離精製法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62148459A true JPS62148459A (ja) | 1987-07-02 |
| JPH0455420B2 JPH0455420B2 (enrdf_load_stackoverflow) | 1992-09-03 |
Family
ID=17751055
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP29004485A Granted JPS62148459A (ja) | 1985-12-23 | 1985-12-23 | グルタミンの分離精製法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62148459A (enrdf_load_stackoverflow) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100404498C (zh) * | 2004-03-15 | 2008-07-23 | 上海化工研究院 | L－谷氨酰胺－15n2的分离提取方法 |
| CN102924321A (zh) * | 2012-11-30 | 2013-02-13 | 通辽梅花生物科技有限公司 | 一种从发酵液中提取谷氨酰胺的方法 |
| CN104860838A (zh) * | 2015-04-29 | 2015-08-26 | 宁夏诚志万胜生物工程有限公司 | 一种从谷氨酰胺发酵液中分离提取谷氨酰胺的方法 |
| CN109438274A (zh) * | 2018-11-19 | 2019-03-08 | 廊坊梅花生物技术开发有限公司 | 从谷氨酰胺粗母液中回收谷氨酰胺的方法 |
-
1985
- 1985-12-23 JP JP29004485A patent/JPS62148459A/ja active Granted
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100404498C (zh) * | 2004-03-15 | 2008-07-23 | 上海化工研究院 | L－谷氨酰胺－15n2的分离提取方法 |
| CN102924321A (zh) * | 2012-11-30 | 2013-02-13 | 通辽梅花生物科技有限公司 | 一种从发酵液中提取谷氨酰胺的方法 |
| CN102924321B (zh) * | 2012-11-30 | 2015-12-09 | 通辽梅花生物科技有限公司 | 一种从发酵液中提取谷氨酰胺的方法 |
| CN104860838A (zh) * | 2015-04-29 | 2015-08-26 | 宁夏诚志万胜生物工程有限公司 | 一种从谷氨酰胺发酵液中分离提取谷氨酰胺的方法 |
| CN109438274A (zh) * | 2018-11-19 | 2019-03-08 | 廊坊梅花生物技术开发有限公司 | 从谷氨酰胺粗母液中回收谷氨酰胺的方法 |
| CN109438274B (zh) * | 2018-11-19 | 2021-09-28 | 廊坊梅花生物技术开发有限公司 | 从谷氨酰胺粗母液中回收谷氨酰胺的方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0455420B2 (enrdf_load_stackoverflow) | 1992-09-03 |
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