JPS61501489A - 認識及び同定剤としてのバクテリオファ−ジ - Google Patents
認識及び同定剤としてのバクテリオファ−ジInfo
- Publication number
- JPS61501489A JPS61501489A JP60501412A JP50141285A JPS61501489A JP S61501489 A JPS61501489 A JP S61501489A JP 60501412 A JP60501412 A JP 60501412A JP 50141285 A JP50141285 A JP 50141285A JP S61501489 A JPS61501489 A JP S61501489A
- Authority
- JP
- Japan
- Prior art keywords
- bacteriophage
- bacteria
- phage
- antibody
- molecular
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- 241000191967 Staphylococcus aureus Species 0.000 description 2
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- 238000010353 genetic engineering Methods 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
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- XNCSCQSQSGDGES-UHFFFAOYSA-N 2-[2-[bis(carboxymethyl)amino]propyl-(carboxymethyl)amino]acetic acid Chemical compound OC(=O)CN(CC(O)=O)C(C)CN(CC(O)=O)CC(O)=O XNCSCQSQSGDGES-UHFFFAOYSA-N 0.000 description 1
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- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
- G01N33/586—Liposomes, microcapsules or cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Wood Science & Technology (AREA)
- Virology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Mycology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Toxicology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US59113684A | 1984-03-19 | 1984-03-19 | |
| US591136 | 1984-03-19 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS61501489A true JPS61501489A (ja) | 1986-07-24 |
Family
ID=24365204
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60501412A Pending JPS61501489A (ja) | 1984-03-19 | 1985-03-15 | 認識及び同定剤としてのバクテリオファ−ジ |
Country Status (7)
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998013515A1 (fr) * | 1996-09-27 | 1998-04-02 | Organo Corporation | Procede et appareil de detection de bacteries |
| JP2003505105A (ja) * | 1999-07-30 | 2003-02-12 | プロフォス・アクチエンゲゼルシャフト | 細菌株の検出および同定 |
| JP2009508490A (ja) * | 2005-09-15 | 2009-03-05 | マイクロファージ・インコーポレーテッド | バクテリオファージを用いた、微生物同定のための方法および装置 |
| JP2012513598A (ja) * | 2008-12-22 | 2012-06-14 | サントル ナシオナル ドゥ ラ ルシェルシェサイアンティフィク（セエヌエールエス） | 新規生体トレーサー及びその濾過設備の制御における使用 |
| JP2018523468A (ja) * | 2015-07-31 | 2018-08-23 | ユニバーシティ オブ ピッツバーグ − オブ ザ コモンウェルス システム オブ ハイヤー エデュケイション | バクテリオファージを使用する細菌の検出 |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4746604A (en) * | 1985-05-24 | 1988-05-24 | Enzo Biochem, Inc. | Specific binding assays utilizing a viable cell as a label |
| AU1043788A (en) * | 1986-12-01 | 1988-06-30 | Mcdonnell Douglas Corporation | Method of identifying unknown organisms |
| JPH05219994A (ja) * | 1990-01-24 | 1993-08-31 | Amoco Corp | シグナル産生部分およびその使用法 |
| GB9326277D0 (en) * | 1993-12-23 | 1994-02-23 | Marconi Gec Ltd | Labelling |
| IT1291913B1 (it) * | 1997-05-22 | 1999-01-21 | Angeletti P Ist Richerche Bio | Metodo che prevede l'uso di batteriofagi per la rivelazione della presenza di molecole di interesse in campioni biologici |
| CA2333463A1 (en) | 1998-06-04 | 1999-12-09 | Microsens Biophage Limited | Analytical method using multiple virus labelling |
| DE60014987T2 (de) * | 1999-02-22 | 2005-11-10 | Matsushita Electric Industrial Co., Ltd., Kadoma | Verfahren zum Nachweis von Bakterien |
| US8216780B2 (en) | 2002-04-12 | 2012-07-10 | Microphage (Tm) Incorporated | Method for enhanced sensitivity in bacteriophage-based diagnostic assays |
| CA2482173C (en) | 2002-04-12 | 2012-10-23 | Colorado School Of Mines | Method for detecting low concentrations of a target bacterium that uses phages to infect target bacterial cells |
| US8092990B2 (en) | 2005-03-31 | 2012-01-10 | Colorado School Of Mines | Apparatus and method for detecting microscopic organisms using bacteriophage |
| AU2008265989B8 (en) | 2007-06-15 | 2012-01-12 | Microphage Incorporated | Method of detection of microorganisms with enhanced bacteriophage amplification |
| US8697434B2 (en) | 2008-01-11 | 2014-04-15 | Colorado School Of Mines | Detection of phage amplification by SERS nanoparticles |
| US9441204B2 (en) | 2008-04-03 | 2016-09-13 | Colorado School Of Mines | Compositions and methods for detecting Yersinia pestis bacteria |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3094466A (en) * | 1959-07-15 | 1963-06-18 | Warner Lambert Pharmaceutical | Preparation for typing of staphylococci and process therefor |
| US3562384A (en) * | 1960-11-08 | 1971-02-09 | Miles Lab | Immunological indicator and test system |
| US3553310A (en) * | 1967-12-28 | 1971-01-05 | Miles Lab | Immunologically reactive particles |
| GB1303802A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1969-01-16 | 1973-01-24 | ||
| US4189466A (en) * | 1975-09-19 | 1980-02-19 | Technical Research Affiliates, Inc. | Detection of rheumatoid factor by antibody sensitized microbial particles |
| US4035316A (en) * | 1975-11-24 | 1977-07-12 | California Institute Of Technology | Cell specific, variable density, polymer microspheres |
| US4104126A (en) * | 1976-01-29 | 1978-08-01 | Nichols Institute Of Endocrinology, Inc. | Non-isotopic substrate assay employing bacteriolysis products |
| US4298689A (en) * | 1978-09-25 | 1981-11-03 | Research Corporation | Gonorrhea diagnostic test |
| US4223005A (en) * | 1979-02-15 | 1980-09-16 | University Of Illinois Foundation | Antibody coated bacteria |
| DE2930706A1 (de) * | 1979-07-28 | 1981-02-05 | Medac Klinische Spezialpraep | Verfahren zum nachweis von erregerspezifischen antikoerpern |
| US4282315A (en) * | 1979-09-13 | 1981-08-04 | Corning Glass Works | Preparation of enriched whole virus radioligand |
| US4474877A (en) * | 1980-09-19 | 1984-10-02 | Research And Education Institute, Inc. Harbor - Ucla Medical Center | Process for detection and measurement of viral specific immunoglobulins and article of manufacture therefor |
| IL63855A (en) * | 1981-09-16 | 1984-10-31 | Teva Pharma | Method and kit for detecting pregnancy |
| US4511662A (en) * | 1982-06-18 | 1985-04-16 | Bio-Rad Laboratories, Inc. | Simultaneous assay for T and B lymphocyte populations and subpopulations |
-
1985
- 1985-03-15 DE DE19853590116 patent/DE3590116T1/de not_active Withdrawn
- 1985-03-15 JP JP60501412A patent/JPS61501489A/ja active Pending
- 1985-03-15 NL NL8520062A patent/NL8520062A/nl unknown
- 1985-03-15 EP EP19850901766 patent/EP0175761A4/en not_active Withdrawn
- 1985-03-15 GB GB08527324A patent/GB2181542A/en not_active Withdrawn
- 1985-03-15 WO PCT/US1985/000438 patent/WO1985004189A1/en not_active Application Discontinuation
- 1985-11-19 SE SE8505458A patent/SE8505458D0/xx not_active Application Discontinuation
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998013515A1 (fr) * | 1996-09-27 | 1998-04-02 | Organo Corporation | Procede et appareil de detection de bacteries |
| JP2003505105A (ja) * | 1999-07-30 | 2003-02-12 | プロフォス・アクチエンゲゼルシャフト | 細菌株の検出および同定 |
| JP2009508490A (ja) * | 2005-09-15 | 2009-03-05 | マイクロファージ・インコーポレーテッド | バクテリオファージを用いた、微生物同定のための方法および装置 |
| JP2012513598A (ja) * | 2008-12-22 | 2012-06-14 | サントル ナシオナル ドゥ ラ ルシェルシェサイアンティフィク（セエヌエールエス） | 新規生体トレーサー及びその濾過設備の制御における使用 |
| JP2018523468A (ja) * | 2015-07-31 | 2018-08-23 | ユニバーシティ オブ ピッツバーグ − オブ ザ コモンウェルス システム オブ ハイヤー エデュケイション | バクテリオファージを使用する細菌の検出 |
Also Published As
| Publication number | Publication date |
|---|---|
| SE8505458L (sv) | 1985-11-19 |
| NL8520062A (nl) | 1986-02-03 |
| GB2181542A (en) | 1987-04-23 |
| WO1985004189A1 (en) | 1985-09-26 |
| SE8505458D0 (sv) | 1985-11-19 |
| DE3590116T1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1987-02-19 |
| EP0175761A4 (en) | 1986-09-24 |
| EP0175761A1 (en) | 1986-04-02 |
| GB8527324D0 (en) | 1985-12-11 |
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