JPS5934882A - バイオセンサ− - Google Patents
バイオセンサ−Info
- Publication number
- JPS5934882A JPS5934882A JP57146707A JP14670782A JPS5934882A JP S5934882 A JPS5934882 A JP S5934882A JP 57146707 A JP57146707 A JP 57146707A JP 14670782 A JP14670782 A JP 14670782A JP S5934882 A JPS5934882 A JP S5934882A
- Authority
- JP
- Japan
- Prior art keywords
- enzyme
- glutamic acid
- present
- electrode
- biosensor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000004190 Enzymes Human genes 0.000 claims abstract description 148
- 108090000790 Enzymes Proteins 0.000 claims abstract description 148
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-Glutamic acid Natural products OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims abstract description 116
- 229960002989 glutamic acid Drugs 0.000 claims abstract description 61
- 239000000126 substance Substances 0.000 claims abstract description 24
- 239000000758 substrate Substances 0.000 claims abstract description 20
- 229940024606 amino acid Drugs 0.000 claims abstract description 18
- 150000001413 amino acids Chemical class 0.000 claims abstract description 17
- 238000004458 analytical method Methods 0.000 claims abstract description 14
- 230000009471 action Effects 0.000 claims abstract description 10
- 230000008859 change Effects 0.000 claims abstract description 10
- 108010093096 Immobilized Enzymes Proteins 0.000 claims description 14
- 108010069325 L-glutamate oxidase Proteins 0.000 claims description 8
- 235000001014 amino acid Nutrition 0.000 abstract description 17
- 102000004316 Oxidoreductases Human genes 0.000 abstract description 8
- 108090000854 Oxidoreductases Proteins 0.000 abstract description 8
- 241000187180 Streptomyces sp. Species 0.000 abstract description 3
- 125000003338 L-glutaminyl group Chemical group O=C([*])[C@](N([H])[H])([H])C([H])([H])C([H])([H])C(=O)N([H])[H] 0.000 abstract 1
- 238000000034 method Methods 0.000 description 45
- 239000012528 membrane Substances 0.000 description 42
- 230000000694 effects Effects 0.000 description 31
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 24
- 108020003175 receptors Proteins 0.000 description 24
- 102000005962 receptors Human genes 0.000 description 24
- 239000002609 medium Substances 0.000 description 23
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 21
- 239000001301 oxygen Substances 0.000 description 21
- 229910052760 oxygen Inorganic materials 0.000 description 21
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- 239000008272 agar Substances 0.000 description 18
- 244000005700 microbiome Species 0.000 description 18
- 239000000243 solution Substances 0.000 description 18
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 16
- 238000006243 chemical reaction Methods 0.000 description 15
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 description 13
- 210000004027 cell Anatomy 0.000 description 12
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- 239000001913 cellulose Substances 0.000 description 12
- -1 mercury ions Chemical class 0.000 description 12
- 239000004220 glutamic acid Substances 0.000 description 11
- 238000004519 manufacturing process Methods 0.000 description 11
- 239000000049 pigment Substances 0.000 description 11
- 238000000502 dialysis Methods 0.000 description 10
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 10
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 9
- 239000000872 buffer Substances 0.000 description 9
- 235000013922 glutamic acid Nutrition 0.000 description 9
- 238000005259 measurement Methods 0.000 description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 8
- 229930195714 L-glutamate Natural products 0.000 description 8
- 239000000523 sample Substances 0.000 description 8
- 239000012488 sample solution Substances 0.000 description 8
- 241000187747 Streptomyces Species 0.000 description 7
- 229910021529 ammonia Inorganic materials 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 6
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 6
- 239000004809 Teflon Substances 0.000 description 6
- 229920006362 Teflon® Polymers 0.000 description 6
- 229960005261 aspartic acid Drugs 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 6
- 230000015572 biosynthetic process Effects 0.000 description 6
- 238000012258 culturing Methods 0.000 description 6
- 230000000813 microbial effect Effects 0.000 description 6
- KPGXRSRHYNQIFN-UHFFFAOYSA-N 2-oxoglutaric acid Chemical compound OC(=O)CCC(=O)C(O)=O KPGXRSRHYNQIFN-UHFFFAOYSA-N 0.000 description 5
- 229920002472 Starch Polymers 0.000 description 5
- 239000008351 acetate buffer Substances 0.000 description 5
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- 229910052697 platinum Inorganic materials 0.000 description 5
- 239000008057 potassium phosphate buffer Substances 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 238000000746 purification Methods 0.000 description 5
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- 239000008107 starch Substances 0.000 description 5
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- 229920000298 Cellophane Polymers 0.000 description 4
- 102000053602 DNA Human genes 0.000 description 4
- 108020004414 DNA Proteins 0.000 description 4
- 108010008292 L-Amino Acid Oxidase Proteins 0.000 description 4
- 102000007070 L-amino-acid oxidase Human genes 0.000 description 4
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- 150000001768 cations Chemical class 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- ORTQZVOHEJQUHG-UHFFFAOYSA-L copper(II) chloride Chemical compound Cl[Cu]Cl ORTQZVOHEJQUHG-UHFFFAOYSA-L 0.000 description 4
- 230000007423 decrease Effects 0.000 description 4
- 229940116901 diethyldithiocarbamate Drugs 0.000 description 4
- LMBWSYZSUOEYSN-UHFFFAOYSA-N diethyldithiocarbamic acid Chemical compound CCN(CC)C(S)=S LMBWSYZSUOEYSN-UHFFFAOYSA-N 0.000 description 4
- 239000003792 electrolyte Substances 0.000 description 4
- 235000019162 flavin adenine dinucleotide Nutrition 0.000 description 4
- 239000011714 flavin adenine dinucleotide Substances 0.000 description 4
- OEZPVSPULCMUQB-VRTOBVRTSA-N hydron;(e)-(3-methyl-1,3-benzothiazol-2-ylidene)hydrazine;chloride Chemical compound Cl.C1=CC=C2S\C(=N\N)N(C)C2=C1 OEZPVSPULCMUQB-VRTOBVRTSA-N 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 235000018102 proteins Nutrition 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- 235000013555 soy sauce Nutrition 0.000 description 4
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- CKLJMWTZIZZHCS-UHFFFAOYSA-N D-OH-Asp Natural products OC(=O)C(N)CC(O)=O CKLJMWTZIZZHCS-UHFFFAOYSA-N 0.000 description 3
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 3
- 239000005057 Hexamethylene diisocyanate Substances 0.000 description 3
- CKLJMWTZIZZHCS-UWTATZPHSA-N L-Aspartic acid Natural products OC(=O)[C@H](N)CC(O)=O CKLJMWTZIZZHCS-UWTATZPHSA-N 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 235000003704 aspartic acid Nutrition 0.000 description 3
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 3
- 238000011088 calibration curve Methods 0.000 description 3
- 239000001569 carbon dioxide Substances 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 238000004132 cross linking Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 125000000524 functional group Chemical group 0.000 description 3
- 230000002068 genetic effect Effects 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- RRAMGCGOFNQTLD-UHFFFAOYSA-N hexamethylene diisocyanate Chemical compound O=C=NCCCCCCN=C=O RRAMGCGOFNQTLD-UHFFFAOYSA-N 0.000 description 3
- 229960002885 histidine Drugs 0.000 description 3
- 239000003094 microcapsule Substances 0.000 description 3
- 239000008363 phosphate buffer Substances 0.000 description 3
- 229910001414 potassium ion Inorganic materials 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 230000004044 response Effects 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 238000011218 seed culture Methods 0.000 description 3
- 229910052709 silver Inorganic materials 0.000 description 3
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- 239000006228 supernatant Substances 0.000 description 3
- 235000015099 wheat brans Nutrition 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 description 2
- XQUPVDVFXZDTLT-UHFFFAOYSA-N 1-[4-[[4-(2,5-dioxopyrrol-1-yl)phenyl]methyl]phenyl]pyrrole-2,5-dione Chemical compound O=C1C=CC(=O)N1C(C=C1)=CC=C1CC1=CC=C(N2C(C=CC2=O)=O)C=C1 XQUPVDVFXZDTLT-UHFFFAOYSA-N 0.000 description 2
- ALYNCZNDIQEVRV-UHFFFAOYSA-N 4-aminobenzoic acid Chemical compound NC1=CC=C(C(O)=O)C=C1 ALYNCZNDIQEVRV-UHFFFAOYSA-N 0.000 description 2
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 239000005909 Kieselgur Substances 0.000 description 2
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 2
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 2
- HDFGOPSGAURCEO-UHFFFAOYSA-N N-ethylmaleimide Chemical compound CCN1C(=O)C=CC1=O HDFGOPSGAURCEO-UHFFFAOYSA-N 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
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- 101710098398 Probable alanine aminotransferase, mitochondrial Proteins 0.000 description 2
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 2
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Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57146707A JPS5934882A (ja) | 1982-08-23 | 1982-08-23 | バイオセンサ− |
| AU16215/83A AU565635B2 (en) | 1982-06-29 | 1983-06-24 | L-glutamic acid oxidase |
| EP83106258A EP0097949B1 (en) | 1982-06-29 | 1983-06-27 | L-glutamic acid oxidase, its production, and its use |
| DE8383106258T DE3379467D1 (en) | 1982-06-29 | 1983-06-27 | L-glutamic acid oxidase, its production, and its use |
| AT83106258T ATE41674T1 (de) | 1982-06-29 | 1983-06-27 | L-glutaminsaeure-oxidase, ihre gewinnung und ihre verwendung. |
| ES523668A ES8500991A1 (es) | 1982-06-29 | 1983-06-28 | Un acido l-glutamico oxidasa. |
| US06/509,234 US4614714A (en) | 1982-08-21 | 1983-06-28 | Use of novel L-glutamic acid oxidase |
| KR1019830002917A KR890002412B1 (ko) | 1982-06-29 | 1983-06-28 | L-글루타민산 옥시다아제의 제조법 |
| CA000431424A CA1208581A (en) | 1982-06-29 | 1983-06-29 | L-glutamic acid oxidase, its production, and its use |
| ES530224A ES8605037A1 (es) | 1982-08-21 | 1984-03-01 | Un metodo de analisis del acido l-glutamico |
| KR1019890005399A KR890003087B1 (ko) | 1982-06-29 | 1989-04-24 | L- 글루타민산의 분석용 키드 및 바이오센서 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57146707A JPS5934882A (ja) | 1982-08-23 | 1982-08-23 | バイオセンサ− |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5934882A true JPS5934882A (ja) | 1984-02-25 |
| JPS6234390B2 JPS6234390B2 (enrdf_load_stackoverflow) | 1987-07-27 |
Family
ID=15413720
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP57146707A Granted JPS5934882A (ja) | 1982-06-29 | 1982-08-23 | バイオセンサ− |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5934882A (enrdf_load_stackoverflow) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2013146264A (ja) * | 2011-12-22 | 2013-08-01 | Okayama Univ | アミノ酸オキシダーゼ固定化体及びアミノ酸測定装置 |
| US9891185B2 (en) | 1998-10-08 | 2018-02-13 | Abbott Diabetes Care Inc. | Small volume in vitro analyte sensor |
| WO2021193598A1 (ja) * | 2020-03-24 | 2021-09-30 | 味の素株式会社 | Ｌ－グルタミン酸オキシダーゼ変異体 |
-
1982
- 1982-08-23 JP JP57146707A patent/JPS5934882A/ja active Granted
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9891185B2 (en) | 1998-10-08 | 2018-02-13 | Abbott Diabetes Care Inc. | Small volume in vitro analyte sensor |
| JP2013146264A (ja) * | 2011-12-22 | 2013-08-01 | Okayama Univ | アミノ酸オキシダーゼ固定化体及びアミノ酸測定装置 |
| WO2021193598A1 (ja) * | 2020-03-24 | 2021-09-30 | 味の素株式会社 | Ｌ－グルタミン酸オキシダーゼ変異体 |
| JPWO2021193598A1 (enrdf_load_stackoverflow) * | 2020-03-24 | 2021-09-30 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6234390B2 (enrdf_load_stackoverflow) | 1987-07-27 |
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