JPS6234390B2 - - Google Patents
Info
- Publication number
- JPS6234390B2 JPS6234390B2 JP57146707A JP14670782A JPS6234390B2 JP S6234390 B2 JPS6234390 B2 JP S6234390B2 JP 57146707 A JP57146707 A JP 57146707A JP 14670782 A JP14670782 A JP 14670782A JP S6234390 B2 JPS6234390 B2 JP S6234390B2
- Authority
- JP
- Japan
- Prior art keywords
- enzyme
- electrode
- glutamic acid
- biosensor
- stable
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000004190 Enzymes Human genes 0.000 claims description 140
- 108090000790 Enzymes Proteins 0.000 claims description 140
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 103
- 238000000034 method Methods 0.000 claims description 51
- 229960002989 glutamic acid Drugs 0.000 claims description 49
- 230000000694 effects Effects 0.000 claims description 39
- 239000000126 substance Substances 0.000 claims description 24
- 239000000758 substrate Substances 0.000 claims description 21
- 238000004458 analytical method Methods 0.000 claims description 16
- 108010093096 Immobilized Enzymes Proteins 0.000 claims description 14
- 229940024606 amino acid Drugs 0.000 claims description 13
- 150000001413 amino acids Chemical class 0.000 claims description 13
- 108010069325 L-glutamate oxidase Proteins 0.000 claims description 12
- 230000009471 action Effects 0.000 claims description 9
- 230000008859 change Effects 0.000 claims description 6
- 239000012847 fine chemical Substances 0.000 claims description 4
- 238000002523 gelfiltration Methods 0.000 claims description 4
- 229920005654 Sephadex Polymers 0.000 claims description 2
- 239000012507 Sephadex™ Substances 0.000 claims description 2
- 239000012528 membrane Substances 0.000 description 43
- 239000002609 medium Substances 0.000 description 25
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 23
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 21
- 239000001301 oxygen Substances 0.000 description 21
- 229910052760 oxygen Inorganic materials 0.000 description 21
- 229920001817 Agar Polymers 0.000 description 18
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- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 16
- 238000006243 chemical reaction Methods 0.000 description 15
- LPUQAYUQRXPFSQ-DFWYDOINSA-M monosodium L-glutamate Chemical compound [Na+].[O-]C(=O)[C@@H](N)CCC(O)=O LPUQAYUQRXPFSQ-DFWYDOINSA-M 0.000 description 14
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- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 10
- 238000005259 measurement Methods 0.000 description 9
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 8
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- 229910021529 ammonia Inorganic materials 0.000 description 7
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- 108010008292 L-Amino Acid Oxidase Proteins 0.000 description 6
- 102000007070 L-amino-acid oxidase Human genes 0.000 description 6
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- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 6
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- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 5
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- VWWQXMAJTJZDQX-UYBVJOGSSA-N flavin adenine dinucleotide Chemical compound C1=NC2=C(N)N=CN=C2N1[C@@H]([C@H](O)[C@@H]1O)O[C@@H]1CO[P@](O)(=O)O[P@@](O)(=O)OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C2=NC(=O)NC(=O)C2=NC2=C1C=C(C)C(C)=C2 VWWQXMAJTJZDQX-UYBVJOGSSA-N 0.000 description 5
- 235000019162 flavin adenine dinucleotide Nutrition 0.000 description 5
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- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 229920000298 Cellophane Polymers 0.000 description 4
- CKLJMWTZIZZHCS-UHFFFAOYSA-N D-OH-Asp Natural products OC(=O)C(N)CC(O)=O CKLJMWTZIZZHCS-UHFFFAOYSA-N 0.000 description 4
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- ORTQZVOHEJQUHG-UHFFFAOYSA-L copper(II) chloride Chemical compound Cl[Cu]Cl ORTQZVOHEJQUHG-UHFFFAOYSA-L 0.000 description 4
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- 238000005516 engineering process Methods 0.000 description 4
- OEZPVSPULCMUQB-VRTOBVRTSA-N hydron;(e)-(3-methyl-1,3-benzothiazol-2-ylidene)hydrazine;chloride Chemical compound Cl.C1=CC=C2S\C(=N\N)N(C)C2=C1 OEZPVSPULCMUQB-VRTOBVRTSA-N 0.000 description 4
- 239000008057 potassium phosphate buffer Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
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- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 4
- KPGXRSRHYNQIFN-UHFFFAOYSA-N 2-oxoglutaric acid Chemical compound OC(=O)CCC(=O)C(O)=O KPGXRSRHYNQIFN-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 3
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 3
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 3
- 229930182816 L-glutamine Natural products 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 3
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- 102000004316 Oxidoreductases Human genes 0.000 description 3
- 108090000854 Oxidoreductases Proteins 0.000 description 3
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- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 238000004132 cross linking Methods 0.000 description 3
- 229940116901 diethyldithiocarbamate Drugs 0.000 description 3
- LMBWSYZSUOEYSN-UHFFFAOYSA-N diethyldithiocarbamic acid Chemical compound CCN(CC)C(S)=S LMBWSYZSUOEYSN-UHFFFAOYSA-N 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
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- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 description 2
- ALYNCZNDIQEVRV-UHFFFAOYSA-N 4-aminobenzoic acid Chemical compound NC1=CC=C(C(O)=O)C=C1 ALYNCZNDIQEVRV-UHFFFAOYSA-N 0.000 description 2
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- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 2
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- 239000005057 Hexamethylene diisocyanate Substances 0.000 description 2
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- 101710098398 Probable alanine aminotransferase, mitochondrial Proteins 0.000 description 2
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 2
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- 239000011701 zinc Substances 0.000 description 1
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57146707A JPS5934882A (ja) | 1982-08-23 | 1982-08-23 | バイオセンサ− |
| AU16215/83A AU565635B2 (en) | 1982-06-29 | 1983-06-24 | L-glutamic acid oxidase |
| EP83106258A EP0097949B1 (en) | 1982-06-29 | 1983-06-27 | L-glutamic acid oxidase, its production, and its use |
| DE8383106258T DE3379467D1 (en) | 1982-06-29 | 1983-06-27 | L-glutamic acid oxidase, its production, and its use |
| AT83106258T ATE41674T1 (de) | 1982-06-29 | 1983-06-27 | L-glutaminsaeure-oxidase, ihre gewinnung und ihre verwendung. |
| ES523668A ES8500991A1 (es) | 1982-06-29 | 1983-06-28 | Un acido l-glutamico oxidasa. |
| US06/509,234 US4614714A (en) | 1982-08-21 | 1983-06-28 | Use of novel L-glutamic acid oxidase |
| KR1019830002917A KR890002412B1 (ko) | 1982-06-29 | 1983-06-28 | L-글루타민산 옥시다아제의 제조법 |
| CA000431424A CA1208581A (en) | 1982-06-29 | 1983-06-29 | L-glutamic acid oxidase, its production, and its use |
| ES530224A ES8605037A1 (es) | 1982-08-21 | 1984-03-01 | Un metodo de analisis del acido l-glutamico |
| KR1019890005399A KR890003087B1 (ko) | 1982-06-29 | 1989-04-24 | L- 글루타민산의 분석용 키드 및 바이오센서 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57146707A JPS5934882A (ja) | 1982-08-23 | 1982-08-23 | バイオセンサ− |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5934882A JPS5934882A (ja) | 1984-02-25 |
| JPS6234390B2 true JPS6234390B2 (enrdf_load_stackoverflow) | 1987-07-27 |
Family
ID=15413720
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP57146707A Granted JPS5934882A (ja) | 1982-06-29 | 1982-08-23 | バイオセンサ− |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5934882A (enrdf_load_stackoverflow) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6338790B1 (en) | 1998-10-08 | 2002-01-15 | Therasense, Inc. | Small volume in vitro analyte sensor with diffusible or non-leachable redox mediator |
| JP2013146264A (ja) * | 2011-12-22 | 2013-08-01 | Okayama Univ | アミノ酸オキシダーゼ固定化体及びアミノ酸測定装置 |
| WO2021193598A1 (ja) * | 2020-03-24 | 2021-09-30 | 味の素株式会社 | Ｌ－グルタミン酸オキシダーゼ変異体 |
-
1982
- 1982-08-23 JP JP57146707A patent/JPS5934882A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5934882A (ja) | 1984-02-25 |
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