JPS59109840A - 走査形電子顕微鏡用生物試料の前処理方法 - Google Patents

走査形電子顕微鏡用生物試料の前処理方法

Info

Publication number
JPS59109840A
JPS59109840A JP57221271A JP22127182A JPS59109840A JP S59109840 A JPS59109840 A JP S59109840A JP 57221271 A JP57221271 A JP 57221271A JP 22127182 A JP22127182 A JP 22127182A JP S59109840 A JPS59109840 A JP S59109840A
Authority
JP
Japan
Prior art keywords
biological sample
inert gas
electron microscope
sample
discharge chamber
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP57221271A
Other languages
English (en)
Japanese (ja)
Inventor
Kintaro Mori
森　金太郎
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Koki Holdings Co Ltd
Original Assignee
Hitachi Koki Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi Koki Co Ltd filed Critical Hitachi Koki Co Ltd
Priority to JP57221271A priority Critical patent/JPS59109840A/ja
Priority to US06/598,511 priority patent/US4727029A/en
Priority to DE3414539A priority patent/DE3414539A1/de
Publication of JPS59109840A publication Critical patent/JPS59109840A/ja
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/44—Sample treatment involving radiation, e.g. heat

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
JP57221271A 1982-12-16 1982-12-16 走査形電子顕微鏡用生物試料の前処理方法 Pending JPS59109840A (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP57221271A JPS59109840A (ja) 1982-12-16 1982-12-16 走査形電子顕微鏡用生物試料の前処理方法
US06/598,511 US4727029A (en) 1982-12-16 1984-04-10 Apparatus and method for the pretreatment of biological specimens for use in scanning electron microscopes
DE3414539A DE3414539A1 (de) 1982-12-16 1984-04-17 Vorrichtung und verfahren zur vorbehandlung von biologischen proben fuer die verwendung in rasterelektronenmikroskopen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP57221271A JPS59109840A (ja) 1982-12-16 1982-12-16 走査形電子顕微鏡用生物試料の前処理方法

Publications (1)

Publication Number Publication Date
JPS59109840A true JPS59109840A (ja) 1984-06-25

Family

ID=16764156

Family Applications (1)

Application Number Title Priority Date Filing Date
JP57221271A Pending JPS59109840A (ja) 1982-12-16 1982-12-16 走査形電子顕微鏡用生物試料の前処理方法

Country Status (3)

Country Link
US (1) US4727029A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
JP (1) JPS59109840A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
DE (1) DE3414539A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4218196A1 (de) * 1992-06-03 1993-12-09 Fraunhofer Ges Forschung Vorrichtung zur Oberflächenbehandlung von Bauteilen mittels Niederdruckplasma
US5412211A (en) * 1993-07-30 1995-05-02 Electroscan Corporation Environmental scanning electron microscope
US5362964A (en) * 1993-07-30 1994-11-08 Electroscan Corporation Environmental scanning electron microscope
US5552601A (en) * 1994-07-13 1996-09-03 Duke University Method of drying cells for scanning electron microscopy
DE19719903A1 (de) * 1997-05-12 1998-11-19 Deutsch Zentr Luft & Raumfahrt Meßvorrichtung und Verfahren zur Reinigung von Kontaminationsbereichen einer Meßvorrichtung
US8981294B2 (en) 2008-07-03 2015-03-17 B-Nano Ltd. Scanning electron microscope, an interface and a method for observing an object within a non-vacuum environment
US20120065092A1 (en) 2010-09-14 2012-03-15 Wai Hobert Fusion analyte cytometric bead assay, and systems and kits for performing the same
CN103270419B (zh) 2010-11-12 2017-12-05 茵赛德斯有限公司 用于通过宫颈细胞的悬液样本预测受试者是否具有宫颈上皮内瘤样(cin)病变的方法和系统
US9466458B2 (en) * 2013-02-20 2016-10-11 B-Nano Ltd. Scanning electron microscope
US20150041062A1 (en) * 2013-08-12 2015-02-12 Lam Research Corporation Plasma processing chamber with removable body
CN104730292B (zh) * 2015-02-12 2017-10-24 天津力神电池股份有限公司 一种锂离子电池嵌锂态负极电镜样品的制备及观测方法
JP2018509621A (ja) 2015-03-12 2018-04-05 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ 紫外線吸収性ポリマー色素およびそれを使用する方法
US9758625B2 (en) 2015-03-12 2017-09-12 Becton, Dickinson And Company Polymeric BODIPY dyes and methods for using the same
US10472521B2 (en) 2015-12-16 2019-11-12 Becton, Dickinson And Company Photostable fluorescent polymeric tandem dyes including luminescent metal complexes
CN109661439A (zh) 2016-07-07 2019-04-19 贝克顿·迪金森公司 发荧光的水溶剂化共轭聚合物
GB201613173D0 (en) 2016-07-29 2016-09-14 Medical Res Council Electron microscopy
CN112611775B (zh) * 2020-12-16 2023-08-11 聚束科技（北京）有限公司 一种用于观察生物组织的方法及电子显微镜
EP4155349A1 (en) 2021-09-24 2023-03-29 Becton, Dickinson and Company Water-soluble yellow green absorbing dyes

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1346301A (en) * 1965-06-04 1974-02-06 Adler Auto Precision Ltd Methods for mixing and or dispensing liquids and apparatus therefor
US3876373A (en) * 1968-03-18 1975-04-08 Nicholas D Glyptis Method and apparatus for modifying the reproductive mechanism of organisms
US3962057A (en) * 1971-06-21 1976-06-08 Ernst Leitz G.M.B.H. Method of imparting contrast to a microscope object
US3775308A (en) * 1972-05-18 1973-11-27 Interior Method for preparation of composite semipermeable membrane
JPS5857205B2 (ja) * 1976-02-02 1983-12-19 住友化学工業株式会社 半透膜の製造方法
JPS5456672A (en) * 1977-10-14 1979-05-07 Toray Ind Inc High polymer tube having modified inner surface
JPS5518403A (en) * 1978-07-25 1980-02-08 Toshiba Corp Formation of organic thin film
US4447374A (en) * 1979-12-05 1984-05-08 The Tokyo Metropolitan Institute Of Medical Science Preparing replica film of specimen for electron microscopy
DE2950454A1 (de) * 1979-12-14 1981-07-02 Tokyo Metropolitan Government, Tokyo Abdruckfilm fuer elektronenmikroskopieverfahren sowie verfahren und vorrichtung zu seiner herstellung

Also Published As

Publication number Publication date
US4727029A (en) 1988-02-23
DE3414539A1 (de) 1985-10-17
DE3414539C2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 1988-03-31

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