JPS5643279A - Novel alkyl substituted fluorescent compound and composite body - Google Patents

Novel alkyl substituted fluorescent compound and composite body

Info

Publication number
JPS5643279A
JPS5643279A JP11015880A JP11015880A JPS5643279A JP S5643279 A JPS5643279 A JP S5643279A JP 11015880 A JP11015880 A JP 11015880A JP 11015880 A JP11015880 A JP 11015880A JP S5643279 A JPS5643279 A JP S5643279A
Authority
JP
Japan
Prior art keywords
alkyl substituted
composite body
fluorescent compound
novel alkyl
substituted fluorescent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP11015880A
Other languages
English (en)
Japanese (ja)
Other versions
JPH0147471B2 (enExample
Inventor
Raru Kuhanna Puyare
Fuitsushiyaa Uruman Edouin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syva Co
Original Assignee
Syva Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syva Co filed Critical Syva Co
Publication of JPS5643279A publication Critical patent/JPS5643279A/ja
Publication of JPH0147471B2 publication Critical patent/JPH0147471B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07J—STEROIDS
    • C07J41/00—Normal steroids containing one or more nitrogen atoms not belonging to a hetero ring
    • C07J41/0033—Normal steroids containing one or more nitrogen atoms not belonging to a hetero ring not covered by C07J41/0005
    • C07J41/0088—Normal steroids containing one or more nitrogen atoms not belonging to a hetero ring not covered by C07J41/0005 containing unsubstituted amino radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
    • C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
    • C07D311/78—Ring systems having three or more relevant rings
    • C07D311/80—Dibenzopyrans; Hydrogenated dibenzopyrans
    • C07D311/82—Xanthenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07J—STEROIDS
    • C07J41/00—Normal steroids containing one or more nitrogen atoms not belonging to a hetero ring
    • C07J41/0033—Normal steroids containing one or more nitrogen atoms not belonging to a hetero ring not covered by C07J41/0005
    • C07J41/0055—Normal steroids containing one or more nitrogen atoms not belonging to a hetero ring not covered by C07J41/0005 the 17-beta position being substituted by an uninterrupted chain of at least three carbon atoms which may or may not be branched, e.g. cholane or cholestane derivatives, optionally cyclised, e.g. 17-beta-phenyl or 17-beta-furyl derivatives
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/961—Chemistry: molecular biology and microbiology including a step of forming, releasing, or exposing the antigen or forming the hapten-immunogenic carrier complex or the antigen per se
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/806—Antigenic peptides or proteins

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Pyrane Compounds (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Saccharide Compounds (AREA)
  • Luminescent Compositions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP11015880A 1979-09-07 1980-08-11 Novel alkyl substituted fluorescent compound and composite body Granted JPS5643279A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/073,158 US4351760A (en) 1979-09-07 1979-09-07 Novel alkyl substituted fluorescent compounds and polyamino acid conjugates

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP1069142A Division JPH01308485A (ja) 1979-09-07 1989-03-20 アルキル置換ケイ光化合物複合体

Publications (2)

Publication Number Publication Date
JPS5643279A true JPS5643279A (en) 1981-04-21
JPH0147471B2 JPH0147471B2 (enExample) 1989-10-13

Family

ID=22112067

Family Applications (2)

Application Number Title Priority Date Filing Date
JP11015880A Granted JPS5643279A (en) 1979-09-07 1980-08-11 Novel alkyl substituted fluorescent compound and composite body
JP1069142A Granted JPH01308485A (ja) 1979-09-07 1989-03-20 アルキル置換ケイ光化合物複合体

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP1069142A Granted JPH01308485A (ja) 1979-09-07 1989-03-20 アルキル置換ケイ光化合物複合体

Country Status (5)

Country Link
US (1) US4351760A (enExample)
EP (1) EP0025912B1 (enExample)
JP (2) JPS5643279A (enExample)
CA (1) CA1159823A (enExample)
DE (1) DE3071680D1 (enExample)

Families Citing this family (143)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4774191A (en) * 1979-09-07 1988-09-27 Syntex (U.S.A.) Inc. Fluorescent conjugates bound to a support
US4588697A (en) * 1979-09-07 1986-05-13 Syntex (U.S.A.) Inc. Method for performing fluorescent protein binding assay employing novel alkyl substituted fluorescent compounds and conjugates
WO1982000641A1 (en) * 1980-08-25 1982-03-04 Ab Kabivitrum Peptide substrates for determination of protease activity
US4439356A (en) * 1981-03-03 1984-03-27 Syva Company Unsymmetrical fluorescein derivatives
IT1140209B (it) * 1981-09-25 1986-09-24 Anic Spa Reagenti per immunoluorescenza e metodo per la loro preparazione
US4952691A (en) * 1981-12-11 1990-08-28 Abbott Laboratories Fluorescence polarization immunoassay
US4585862A (en) * 1981-12-11 1986-04-29 Abbott Laboratories Fluorescence polarization immunoassay
US4537718A (en) * 1982-10-26 1985-08-27 Columbia University In The City Of New York Impermeant spectroscopic probes
US4582791A (en) * 1983-10-07 1986-04-15 Syntex (U.S.A.) Inc. Reducing non-specific background in immunofluorescence techniques
US5336622A (en) * 1986-04-25 1994-08-09 Abbott Laboratories Tracers for use in flecainide fluorescence polarization immunoassay
US5124457A (en) * 1986-05-21 1992-06-23 Abbott Laboratories Phencyclidine and phencyclidine metabolites assay, tracers, immunogens and antibodies
US5221629A (en) * 1986-05-21 1993-06-22 Abbott Laboratories Phencyclidine and phencyclidine metabolites assay, tracers, immunogens and antibodies
US5155212A (en) * 1986-05-21 1992-10-13 Abbott Laboratories Phencyclidine and phencyclidine metabolites assay, tracers, immunogens, antibodies and reagent kit
US4939264A (en) * 1986-07-14 1990-07-03 Abbott Laboratories Immunoassay for opiate alkaloids and their metabolites; tracers, immunogens and antibodies
US5223627A (en) * 1986-07-15 1993-06-29 Abbott Laboratories Fluorescence polarization method for monitoring fetal lung maturity
US4784961A (en) * 1987-03-23 1988-11-15 Abbott Laboratories Fluorescence polarization method for monitoring fetal lung maturity
US5034317A (en) * 1987-01-30 1991-07-23 Polaroid Corporation Enzyme controlled release system and organic conjugate reactant
US4868132A (en) * 1987-02-03 1989-09-19 Abbott Laboratories Fluorescence polarization immunoassay for amphetamine/methamphetamine
US4900686A (en) * 1987-04-02 1990-02-13 Polaroid Corporation Fluorescent conjugates and biological diagnostic assay
US5100807A (en) * 1987-10-19 1992-03-31 Abbott Laboratories Phenylacetylglutamine (pag) analytical test
US5262333A (en) * 1988-10-28 1993-11-16 Abbott Laboratories Method and reagents for detecting amphetamine and/or D-methamphetamine in biological samples
US5073629A (en) * 1989-01-23 1991-12-17 Abbott Laboratories Methadone fluorescence polarization immunoassay
EP0386644B1 (en) * 1989-03-10 1997-06-04 F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft Reagents for the determination of drugs
US5248791A (en) * 1989-04-10 1993-09-28 Abbott Laboratories Reagents, methods and kits for an amphetamine-class fluorescence polarization immunoassay
US5101015A (en) * 1989-04-10 1992-03-31 Abbott Laboratories Reagents for an amphetamine-class fluorescence polarization immunoassay
US5099020A (en) * 1989-11-27 1992-03-24 Abbott Laboratories Barbiturate assay compositions and methods
US5614386A (en) * 1995-06-23 1997-03-25 Baylor College Of Medicine Alternative dye-labeled primers for automated DNA sequencing
US5728529A (en) * 1995-06-23 1998-03-17 Baylor College Of Medicine Alternative dye-labeled ribonucleotides, deoxyribonucleotides, and dideoxyribonucleotides for automated DNA analysis
AU2678997A (en) * 1996-04-19 1997-11-12 Lexigen Pharmaceuticals Corp. Inhibition of the binding of human ige to its receptor by tetracyclic compounds for the alleviation of ige-mediated immune response
WO1998035012A2 (en) 1997-02-12 1998-08-13 Chan Eugene Y Methods and products for analyzing polymers
US6544797B1 (en) 1997-04-09 2003-04-08 Biosite Diagnostics, Inc. Compositions and methods for inhibiting light-induced inactivation of biological reagents
US6683173B2 (en) 1998-04-03 2004-01-27 Epoch Biosciences, Inc. Tm leveling methods
US7045610B2 (en) * 1998-04-03 2006-05-16 Epoch Biosciences, Inc. Modified oligonucleotides for mismatch discrimination
US7715989B2 (en) * 1998-04-03 2010-05-11 Elitech Holding B.V. Systems and methods for predicting oligonucleotide melting temperature (TmS)
US6949367B1 (en) 1998-04-03 2005-09-27 Epoch Pharmaceuticals, Inc. Modified oligonucleotides for mismatch discrimination
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6153411A (en) 1998-10-30 2000-11-28 American Water Works Company, Inc. Methods and kits for detection of Cryptosporidium parvum using immunomagnetic separation and amplification
WO2000063683A1 (en) 1999-04-20 2000-10-26 Target Discovery, Inc. Polypeptide fingerprinting methods, metabolic profiling, and bioinformatics database
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
EP1975256B1 (en) 2000-03-01 2012-07-11 Epoch Biosciences, Inc. Modified oligonucleotides for mismatch discrimination
EP1944310A3 (en) 2000-03-01 2008-08-06 Epoch Biosciences, Inc. Modified oligonucleotides for mismatch discrimination
US7019129B1 (en) 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
US6936702B2 (en) * 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
WO2001094609A1 (en) 2000-06-07 2001-12-13 Li-Cor, Inc. Charge-switch nucleotides
US7163918B2 (en) * 2000-08-22 2007-01-16 New River Pharmaceuticals Inc. Iodothyronine compositions
US20040209251A1 (en) 2000-10-19 2004-10-21 Schneider Luke V Methods for determining protein and peptide terminal sequences
EP1354064A2 (en) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
EP1368497A4 (en) * 2001-03-12 2007-08-15 California Inst Of Techn METHODS AND APPARATUS FOR ANALYZING ASYNCHRONOUS BASE EXTENSION POLYNUCLEOTIDE SEQUENCES
NZ511705A (en) * 2001-05-14 2004-03-26 Horticulture & Food Res Inst Methods and rapid immunoassay device for detecting progesterone and other steroids
US7118907B2 (en) * 2001-06-06 2006-10-10 Li-Cor, Inc. Single molecule detection systems and methods
US6800765B2 (en) * 2001-08-02 2004-10-05 Molecular Devices Corporation Fluorescent pH indicators for intracellular assays
US20040054162A1 (en) * 2001-10-30 2004-03-18 Hanna Michelle M. Molecular detection systems utilizing reiterative oligonucleotide synthesis
US7045319B2 (en) * 2001-10-30 2006-05-16 Ribomed Biotechnologies, Inc. Molecular detection systems utilizing reiterative oligonucleotide synthesis
EP1497304B1 (en) 2002-04-12 2014-06-25 Catalyst Assets LLC Dual-labeled nucleotides
AU2003291677A1 (en) * 2002-10-30 2004-05-25 Pointilliste, Inc. Methods for producing polypeptide-tagged collections and capture systems containing the tagged polypeptides
WO2004071641A2 (en) * 2003-02-10 2004-08-26 Pointilliste, Inc. Self-assembling arrays and uses thereof
US7745116B2 (en) * 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
CA2544367A1 (en) * 2003-10-29 2005-05-12 Ribomed Biotechnologies, Inc. Compositions, methods and detection technologies for reiterative oligonucleotide synthesis
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005067980A2 (en) * 2004-01-12 2005-07-28 Pointilliste, Inc. Design of therapeutics and therapeutics
EP1716254B1 (en) 2004-02-19 2010-04-07 Helicos Biosciences Corporation Methods for analyzing polynucleotide sequences
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
JP2008512084A (ja) 2004-05-25 2008-04-24 ヘリコス バイオサイエンシーズ コーポレイション 核酸の配列決定のための方法およびデバイス
US20060067889A1 (en) * 2004-09-27 2006-03-30 Light Sciences Corporation Singlet oxygen photosensitizers activated by target binding enhancing the selectivity of targeted PDT agents
US7220549B2 (en) * 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7842823B2 (en) * 2005-10-27 2010-11-30 The Regents Of The University Of California Fluorogenic probes for reactive oxygen species
WO2007070642A2 (en) * 2005-12-15 2007-06-21 Helicos Biosciences Corporation Methods for increasing accuracy of nucleic acid sequencing
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US20090092967A1 (en) * 2006-06-26 2009-04-09 Epoch Biosciences, Inc. Method for generating target nucleic acid sequences
US8557601B2 (en) 2006-07-10 2013-10-15 The Regents Of The University Of California Luminescent 1-hydroxy-2-pyridinone chelates of lanthanides
JP5352460B2 (ja) 2006-08-15 2013-11-27 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア ルミネッセント大環状ランタニド錯体
FR2918666B1 (fr) * 2007-07-13 2010-12-31 Synth Innove Lab Colorants fluorescents polycarboxyliques
CN102037144B (zh) 2008-04-01 2013-09-25 生物检索技术股份有限公司 稳定化的核酸暗猝灭剂-荧光团探针
US8242243B2 (en) 2008-05-15 2012-08-14 Ribomed Biotechnologies, Inc. Methods and reagents for detecting CpG methylation with a methyl CpG binding protein (MBP)
WO2009152102A2 (en) * 2008-06-10 2009-12-17 The Regents Of The University Of California Pro-fluorescent probes
US8211644B2 (en) * 2008-07-13 2012-07-03 Ribomed Biotechnologies, Inc. Molecular beacon-based methods for detection of targets using abscription
US20120034156A1 (en) * 2010-08-03 2012-02-09 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Artificial cells
US8583380B2 (en) 2008-09-05 2013-11-12 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
WO2010107716A2 (en) 2009-03-15 2010-09-23 Ribomed Biotechnologies, Inc. Abscription based molecular detection
US9273059B2 (en) 2009-08-24 2016-03-01 Lumiphore, Inc. Macrocyclic HOPO chelators
WO2011027966A2 (en) 2009-09-03 2011-03-10 Seegene, Inc. Td probe and its uses
KR20110050327A (ko) 2009-11-07 2011-05-13 주식회사 씨젠 Ｔｈｄ 프라이머 타겟 검출
BR112012018394B8 (pt) 2009-12-21 2021-07-27 Seegene Inc método para detecção de uma sequência de ácido nucleico alvo e kit para detecção de uma sequência de ácido nucleico alvo
US20110151457A1 (en) 2009-12-22 2011-06-23 Elitech Holding B.V. Hypertheromostable endonuclease iv substrate probe
CN103228798B (zh) 2010-09-24 2015-12-09 斯坦福大学托管董事会 使用固定的引物直接捕获、扩增及测序靶标dna
KR20120042100A (ko) 2010-10-22 2012-05-03 주식회사 씨젠 이중표지 고정화 프로브를 이용한 고상에서의 타겟 핵산서열 검출
MX340258B (es) 2011-01-11 2016-07-01 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
US8969003B2 (en) 2011-03-23 2015-03-03 Elitech Holding B.V. Functionalized 3-alkynyl pyrazolopyrimidine analogues as universal bases and methods of use
WO2012129547A1 (en) 2011-03-23 2012-09-27 Elitech Holding B.V. Functionalized 3-alkynyl pyrazolopyrimidine analogues as universal bases and methods of use
KR101569479B1 (ko) 2011-03-29 2015-11-16 주식회사 씨젠 Ｐｔｏ 절단 및 연장-의존적 절단에 의한 타겟 핵산서열의 검출
MX342067B (es) 2011-05-04 2016-09-09 Seegene Inc Detección de secuencias de ácido nucleico objetivo por desdoblamiento e hibridización de oligonucleótido de sonda.
US8809238B2 (en) 2011-05-09 2014-08-19 Fluidigm Corporation Probe based nucleic acid detection
DK2714939T3 (en) 2011-05-24 2015-06-29 Elitech Holding Bv DETECTION OF METHICILLIN-RESISTENT STAPHYLOCOCCUS AUREUS
JP2015513666A (ja) 2012-02-17 2015-05-14 エヌブイエス テクノロジーズ，インコーポレイティド アッセイへの適用のためのポリマー骨格
RU2620955C2 (ru) 2012-03-05 2017-05-30 Сиджен, Инк. Детекция нуклеотидной вариации в нуклеиновокислотной последовательности-мишени в анализе с расщеплением и удлинением зондирующего и метящего олигонуклеотида (рто)
ES2833524T3 (es) 2012-05-02 2021-06-15 Ibis Biosciences Inc Secuenciación de ADN
ES2683707T3 (es) 2012-05-02 2018-09-27 Ibis Biosciences, Inc. Secuenciación de ADN
EP2850086B1 (en) 2012-05-18 2023-07-05 Pacific Biosciences Of California, Inc. Heteroarylcyanine dyes
WO2014035712A1 (en) 2012-08-28 2014-03-06 Pierce Biotechnology, Inc. Benzopyrylium compounds
SG11201502786TA (en) 2012-10-12 2015-05-28 Nvs Technologies Inc Polymers having orthogonal reactive groups and uses thereof
US20160046997A1 (en) 2012-10-18 2016-02-18 Oslo Universitetssykehus Hf Biomarkers for cervical cancer
WO2014078733A1 (en) 2012-11-16 2014-05-22 The Regents Of The University Of California Pictet-spengler ligation for protein chemical modification
US10077475B2 (en) 2013-01-24 2018-09-18 California Institute Of Technology FRET-based analytes detection and related methods and systems
EP4570924A3 (en) 2013-01-24 2025-09-03 California Institute of Technology Chromophore-based characterization and detection methods
AU2014200958B2 (en) 2013-02-25 2016-01-14 Seegene, Inc. Detection of nucleotide variation on target nucleic acid sequence
EP2971106B1 (en) 2013-03-11 2020-02-26 ELITechGroup, Inc. Methods for true isothermal strand displacement amplification
WO2014142575A1 (en) 2013-03-13 2014-09-18 Seegene, Inc. Quantification of target nucleic acid using melting peak analysis
WO2014159063A1 (en) 2013-03-14 2014-10-02 Elitech Holding B.V. Functionalized 3-alkynyl pyrazolopyrimidine analogues as universal bases and methods of use
BR112015023069A2 (pt) 2013-03-14 2017-07-18 Nvs Tech Inc superfície de oxidação para o sequestro de biomoléculas e métodos relacionados
US9347095B2 (en) 2013-03-15 2016-05-24 Bio-Rad Laboratories, Inc. Digital assays for mutation detection
KR101403507B1 (ko) 2013-03-21 2014-06-09 주식회사 현일바이오 결핵균 및 비결핵 마이코박테리아의 선택적 검출 방법, 그리고 이를 이용한 키트
EP2997161B1 (en) 2013-05-13 2017-09-27 Elitechgroup B.V. Droplet digital pcr with short minor groove probes
WO2015008985A1 (en) 2013-07-15 2015-01-22 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent immobilized oligonucleotide hybridization
WO2015057008A2 (en) 2013-10-18 2015-04-23 Seegene, Inc. Detection of target nucleic acid sequence on solid phase by pto cleavage and extension using hcto assay
US9540688B2 (en) 2014-02-19 2017-01-10 Personal Genomics, Inc. Real-time sequencing method for single molecule
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
EP3140286B1 (en) 2014-05-09 2018-12-26 Biosearch Technologies, Inc. Cosmic quenchers
EP3230467A1 (en) 2014-12-12 2017-10-18 ELITechGroup B.V. Methods and compositions for detecting antibiotic resistant bacteria
JP2017538418A (ja) 2014-12-12 2017-12-28 エリテックグループ・ベスローテン・フェンノートシャップＥｌｉｔｅｃｈｇｒｏｕｐ Ｂ．Ｖ． 抗生物質耐性細菌を検出する方法および組成物
EP3280821B1 (en) 2015-04-07 2023-12-06 Polyskope Labs Detection of one or more pathogens
PT3283499T (pt) 2015-04-15 2022-08-23 Biosearch Tech Inc Sondas de extintor dual
DK3298162T3 (da) 2015-05-18 2020-03-02 Saga Diagnostics Ab Detektering af målnukleinsyre og varianter
JP7208797B2 (ja) 2016-07-01 2023-01-19 カールスバーグ エー／エス プーリング及び分割アプローチを適用することによって生物の集団内の突然変異体をスクリーニングする方法
EP4575499A3 (en) 2017-01-04 2025-07-09 MGI Tech Co., Ltd. Stepwise sequencing by non-labeled reversible terminators or natural nucleotides
CN108823287B (zh) 2017-04-28 2019-04-12 厦门大学 一种检测靶核酸序列的方法
US11591645B2 (en) 2017-08-31 2023-02-28 Seegene, Inc. Evaluation of performance of components using a pair of dimer-forming primers
WO2019066461A2 (en) 2017-09-29 2019-04-04 Seegene, Inc. DETECTION OF TARGET NUCLEIC ACID SEQUENCES BY CLEAVAGE ANALYSIS AND EXTENSION OF PTO
WO2019203623A1 (en) 2018-04-20 2019-10-24 Seegene, Inc. . Method and apparatus for detecting a plurality of target nucleic acid sequences in sample
KR102220701B1 (ko) 2018-09-28 2021-03-03 (주)나노헬릭스 형광기반의 다중 융해 분석을 이용한 증폭 산물의 다중 분석방법
EP4040946A1 (en) 2019-10-10 2022-08-17 Carlsberg A/S Methods for preparing mutant plants
WO2021080629A1 (en) 2019-10-23 2021-04-29 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
CN113046420B (zh) 2019-12-26 2022-10-04 厦门大学 一种不对称扩增多个靶核酸的方法
CN113046421B (zh) 2019-12-26 2022-09-30 厦门大学 一种不对称扩增靶核酸的方法
EP4403645A4 (en) 2021-09-17 2025-10-08 Seegene Inc DETECTION OF A TARGET NUCLEIC ACID SEQUENCE USING A MARKER OLIGONUCLEOTIDE CARRYING A SYNTHETIC NON-NATURAL BASE
CA3229091A1 (en) 2021-10-26 2023-05-04 Caribou Biosciences, Inc. Exonuclease-coupled real-time endonuclease activity assay
EP4446438A4 (en) 2021-12-10 2025-10-08 Seegene Inc LABELED LOOP PRIMER COMPRISING A SINGLE-STRANDED INTERACTIVE DOUBLE LABELING SYSTEM, AND NUCLEIC ACID ISOTHERMAL AMPLIFICATION METHOD USING SAME
KR20250034099A (ko) 2022-06-27 2025-03-10 주식회사 씨젠 베타-하이드록시산을 이용한 타겟 핵산의 증폭 방법
EP4603593A1 (en) 2022-10-14 2025-08-20 Seegene, Inc. Method for detecting target nucleic acid in sample using two probes with different tm values
EP4621068A1 (en) 2022-11-16 2025-09-24 Seegene, Inc. Detection of target nucleic acid by quenching-pto cleavage and extension (q-ptoce) assay
WO2026050087A1 (en) 2024-08-27 2026-03-05 Caribou Biosciences, Inc. Real-time crispr endonuclease activity assay

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS518673A (en) * 1974-07-12 1976-01-23 Kurita Machinery Manuf Fuirutaapuresuno senjosochi
US3996345A (en) * 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US3998943A (en) * 1973-10-02 1976-12-21 Syva Company Double receptor fluorescent immunoassay
US4199559A (en) * 1974-08-12 1980-04-22 Syva Company Fluorescence quenching with immunological pairs in immunoassays

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1417382A (en) * 1973-05-08 1975-12-10 Fuji Photo Film Co Ltd Heat-developable photographic material
US4174384A (en) * 1975-06-30 1979-11-13 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4230805A (en) * 1977-04-15 1980-10-28 Syva Company Theophylline antigens and antibodies
DE2824917A1 (de) * 1977-07-13 1979-02-01 Corning Glass Works Biologisch aktive konjugate
US4220450A (en) * 1978-04-05 1980-09-02 Syva Company Chemically induced fluorescence immunoassay
US4193983A (en) * 1978-05-16 1980-03-18 Syva Company Labeled liposome particle compositions and immunoassays therewith
US4318846A (en) * 1979-09-07 1982-03-09 Syva Company Novel ether substituted fluorescein polyamino acid compounds as fluorescers and quenchers

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3998943A (en) * 1973-10-02 1976-12-21 Syva Company Double receptor fluorescent immunoassay
JPS518673A (en) * 1974-07-12 1976-01-23 Kurita Machinery Manuf Fuirutaapuresuno senjosochi
US3996345A (en) * 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4199559A (en) * 1974-08-12 1980-04-22 Syva Company Fluorescence quenching with immunological pairs in immunoassays

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JPH01308485A (ja) 1989-12-13
EP0025912B1 (en) 1986-07-30
DE3071680D1 (en) 1986-09-04
EP0025912A1 (en) 1981-04-01
JPH0147471B2 (enExample) 1989-10-13
CA1159823A (en) 1984-01-03
US4351760A (en) 1982-09-28
JPH034596B2 (enExample) 1991-01-23

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