JPH11508775A - ヘベアブラシリエンシス(Heveabrasiliensis)から得られる(S)−ヒドロキシニトリルリアーゼ - Google Patents
ヘベアブラシリエンシス(Heveabrasiliensis)から得られる(S)−ヒドロキシニトリルリアーゼInfo
- Publication number
- JPH11508775A JPH11508775A JP9505502A JP50550297A JPH11508775A JP H11508775 A JPH11508775 A JP H11508775A JP 9505502 A JP9505502 A JP 9505502A JP 50550297 A JP50550297 A JP 50550297A JP H11508775 A JPH11508775 A JP H11508775A
- Authority
- JP
- Japan
- Prior art keywords
- protein
- hnl
- hydroxynitrile lyase
- dna sequence
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/002—Nitriles (-CN)
- C12P13/004—Cyanohydrins
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Detergent Compositions (AREA)
- Chemical Or Physical Treatment Of Fibers (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Saccharide Compounds (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.精製されかつ単離された形の(S)-ヒドロキシニトリルリアーゼ。 2.後続のDNA配列SEQ ID NO1に由来するアミノ酸配列SEQ I D NO2を有するか又は少なくとも80%がこの配列と同一である、請求の範 囲1記載の(S)-ヒドロキシニトリルリアーゼ。 3.(S)-ヒドロキシニトリルリアーゼをコードするか又はヒドロキシニトリル リアーゼをコードする領域中で85%以上がこの配列と同一である、請求の範囲 2記載のDNA配列。 4.(S)-ヒドロキシニトリルリアーゼ活性に必要な配列を有する、請求の範囲 3記載のDNA配列。 5.請求の範囲3又は4記載のDNA配列を有するベクター。 6.ベクターによって導入される、請求の範囲3又は4記載のDNA配列を有す る宿主細胞。 7.微生物の細胞である、請求の範囲6記載の宿主細胞。 8.サッカロミセス セレビシアエ(Saccharomyces cerevisiae)又はピチアパ ストリス(Pichia pastoris)に由来する、請求の範囲6又は7記載の宿主細胞 。 9.請求の範囲6ないし8のいずれかに記載の宿主細胞中での請求の範囲3又は 4記載のDNA配列の不均一な発現によって産生される組換えたん白質。 10.(S)-ヒドロキシニトリルリアーゼ活性を有するたん白質を産生する方法に 於て、請求の範囲6ないし8のいずれかに記載の宿主細胞を培養し、産生したた ん白質を単離することを特徴とする、上記方法。 11.(S)-シアンヒドリンを産生するために、請求の範囲9記載の組換えたん白 質を使用する方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AT0118295A AT404837B (de) | 1995-07-12 | 1995-07-12 | (s)-hydroxynitrillyase aus hevea brasiliensis |
AT1182/95 | 1995-07-12 | ||
PCT/EP1996/003010 WO1997003204A2 (de) | 1995-07-12 | 1996-07-10 | (s)-hydroxynitrillyase aus hevea brasiliensis |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH11508775A true JPH11508775A (ja) | 1999-08-03 |
JP4090076B2 JP4090076B2 (ja) | 2008-05-28 |
Family
ID=3508388
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP50550297A Expired - Lifetime JP4090076B2 (ja) | 1995-07-12 | 1996-07-10 | ヘベアブラシリエンシス(Heveabrasiliensis)から得られる(S)−ヒドロキシニトリルリアーゼ |
Country Status (11)
Country | Link |
---|---|
US (1) | US6046042A (ja) |
EP (1) | EP0840792B1 (ja) |
JP (1) | JP4090076B2 (ja) |
AT (2) | AT404837B (ja) |
CA (1) | CA2226518A1 (ja) |
DE (1) | DE59608804D1 (ja) |
DK (1) | DK0840792T3 (ja) |
ES (1) | ES2170251T3 (ja) |
IL (1) | IL122606A (ja) |
TW (1) | TW469293B (ja) |
WO (1) | WO1997003204A2 (ja) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2005312431A (ja) * | 2004-03-31 | 2005-11-10 | Nippon Shokubai Co Ltd | 新規改変型s−ヒドロキシニトリルリアーゼ |
WO2006041226A1 (ja) | 2004-10-15 | 2006-04-20 | Mitsubishi Rayon Co., Ltd. | 改良型ヒドロキシニトリルリアーゼ |
US7214523B2 (en) | 2002-12-17 | 2007-05-08 | Nippon Shokubai Co., Ltd. | Method for production of S-hydroxynitrile lyase by use of Escherichia coli |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19703314A1 (de) * | 1996-02-09 | 1997-08-14 | Degussa | Verfahren zur Herstellung von (S)-Cyanhydrinen |
AT407397B (de) | 1998-07-02 | 2001-02-26 | Dsm Fine Chem Austria Gmbh | (s)-hydroxynitrillyasen mit verbesserter substratakzeptanz und deren verwendung |
DE69928206T2 (de) * | 1998-12-28 | 2006-08-03 | Nippon Shokubai Co., Ltd. | Verfahren zur Herstellung von S-Hydroxynitrillyasen |
US6194039B1 (en) * | 1999-10-22 | 2001-02-27 | Elsicon, Inc. | Materials for inducing alignment in liquid crystals and liquid crystal displays |
DE19963485B4 (de) * | 1999-12-28 | 2006-02-16 | JFC - Jülich Fine Chemicals GmbH | Verfahren zur Herstellung von Hydroxylnitril-Lyasen |
ATE423209T1 (de) * | 2001-01-16 | 2009-03-15 | Dsm Fine Chem Austria Gmbh | Gene, enthaltend eine für hydroxynitrillyase codierende dna-sequenz, rekombinante proteine mit hydroxynitrillyase-aktivität und deren verwendung |
AT412157B (de) * | 2002-07-15 | 2004-10-25 | Dsm Fine Chem Austria Gmbh | Screeningmethode zum nachweis von amidase- und nitrilhydrataseaktivitäten und deren verwendung |
AT412092B (de) * | 2003-02-27 | 2004-09-27 | Dsm Fine Chem Austria Gmbh | Verfahren zur herstellung chiraler alpha-hydroxycarbonsäuren durch enzymatische hydrolyse von chiralen cyanhydrinen |
EP1730274A4 (en) * | 2004-03-31 | 2008-03-26 | Nippon Catalytic Chem Ind | NOVEL MODIFIED S-HYDROXYNITRIC LYASE |
CN101611141B (zh) | 2006-12-14 | 2014-05-07 | 帝斯曼知识产权资产管理有限公司 | R-hnl随机变体及其用于制备光学纯的空间位阻氰醇的用途 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AT396252B (de) * | 1991-10-31 | 1993-07-26 | Chemie Linz Gmbh | Enzymatisches verfahren zur enantioselektiven herstellung optisch aktiver cyanhydrine |
-
1995
- 1995-07-12 AT AT0118295A patent/AT404837B/de not_active IP Right Cessation
-
1996
- 1996-07-10 WO PCT/EP1996/003010 patent/WO1997003204A2/de active IP Right Grant
- 1996-07-10 AT AT96925698T patent/ATE213776T1/de not_active IP Right Cessation
- 1996-07-10 DE DE59608804T patent/DE59608804D1/de not_active Expired - Lifetime
- 1996-07-10 IL IL12260696A patent/IL122606A/xx not_active IP Right Cessation
- 1996-07-10 ES ES96925698T patent/ES2170251T3/es not_active Expired - Lifetime
- 1996-07-10 US US08/981,256 patent/US6046042A/en not_active Expired - Lifetime
- 1996-07-10 CA CA002226518A patent/CA2226518A1/en not_active Abandoned
- 1996-07-10 DK DK96925698T patent/DK0840792T3/da active
- 1996-07-10 JP JP50550297A patent/JP4090076B2/ja not_active Expired - Lifetime
- 1996-07-10 EP EP96925698A patent/EP0840792B1/de not_active Expired - Lifetime
- 1996-07-11 TW TW085108400A patent/TW469293B/zh not_active IP Right Cessation
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7214523B2 (en) | 2002-12-17 | 2007-05-08 | Nippon Shokubai Co., Ltd. | Method for production of S-hydroxynitrile lyase by use of Escherichia coli |
JP2005312431A (ja) * | 2004-03-31 | 2005-11-10 | Nippon Shokubai Co Ltd | 新規改変型s−ヒドロキシニトリルリアーゼ |
WO2006041226A1 (ja) | 2004-10-15 | 2006-04-20 | Mitsubishi Rayon Co., Ltd. | 改良型ヒドロキシニトリルリアーゼ |
EP2243831A1 (en) | 2004-10-15 | 2010-10-27 | Mitsubishi Rayon Co., Ltd. | Improved hydroxynitrile lyase |
EP2267124A1 (en) | 2004-10-15 | 2010-12-29 | Mitsubishi Rayon Co., Ltd. | Improved hydroxynitrile lyase |
Also Published As
Publication number | Publication date |
---|---|
TW469293B (en) | 2001-12-21 |
ATA118295A (de) | 1998-07-15 |
EP0840792B1 (de) | 2002-02-27 |
AT404837B (de) | 1999-03-25 |
JP4090076B2 (ja) | 2008-05-28 |
WO1997003204A3 (de) | 1997-03-20 |
DK0840792T3 (da) | 2002-04-22 |
WO1997003204A2 (de) | 1997-01-30 |
DE59608804D1 (en) | 2002-04-04 |
IL122606A (en) | 2005-11-20 |
ES2170251T3 (es) | 2002-08-01 |
US6046042A (en) | 2000-04-04 |
ATE213776T1 (de) | 2002-03-15 |
IL122606A0 (en) | 1998-08-16 |
CA2226518A1 (en) | 1997-01-30 |
EP0840792A2 (de) | 1998-05-13 |
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