JPH09509311A - 単離p27タンパク質及びそれをコードする核酸分子 - Google Patents
単離p27タンパク質及びそれをコードする核酸分子Info
- Publication number
- JPH09509311A JPH09509311A JP7518645A JP51864595A JPH09509311A JP H09509311 A JPH09509311 A JP H09509311A JP 7518645 A JP7518645 A JP 7518645A JP 51864595 A JP51864595 A JP 51864595A JP H09509311 A JPH09509311 A JP H09509311A
- Authority
- JP
- Japan
- Prior art keywords
- cyclin
- cdk2
- protein
- complex
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- NGSWKAQJJWESNS-ZZXKWVIFSA-N trans-4-coumaric acid Chemical compound OC(=O)\C=C\C1=CC=C(O)C=C1 NGSWKAQJJWESNS-ZZXKWVIFSA-N 0.000 description 1
- KHPCPRHQVVSZAH-UHFFFAOYSA-N trans-cinnamyl beta-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OCC=CC1=CC=CC=C1 KHPCPRHQVVSZAH-UHFFFAOYSA-N 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 230000014621 translational initiation Effects 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
- 238000005199 ultracentrifugation Methods 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
- 241000712461 unidentified influenza virus Species 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 229940054870 urso Drugs 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4738—Cell cycle regulated proteins, e.g. cyclin, CDC, INK-CCR
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Zoology (AREA)
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- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.SDSポリアクリルアミドゲル電気泳動により測定される約27kDの見掛 けの分子量を有し、サイクリンE−Cdk2複合体に結合し、その活性化を阻害 することができる単離タンパク質。 2.請求の範囲第1項のタンパク質をコードする組み換え核酸分子。 3.核酸分子がDNA分子である請求の範囲第2項の組み換え核酸分子。 4.DNA分子がcDNA分子である請求の範囲第3項の組み換え核酸分子。 5.核酸分子がRNA分子である請求の範囲第2項の組み換え核酸分子。 6.請求の範囲第4項の組み換え核酸分子を含むベクター。 7.ベクターがプラスミドである請求の範囲第6項のベクター。 8.ベクターがウィルスである請求の範囲第6項のベクター。 9.適した宿主中に請求の範囲第6項に記載のベクターを含む、SDSポリアク リルアミドゲル電気泳動により測定される約27kDの見掛けの分子量を有し、 サイクリンE−Cdk2複合体に結合し、その活性化を阻害することができるタ ンパク質を製造するための宿主ベクター系。 10.適した宿主がバクテリア細胞である請求の範囲第9項の宿主ベクター系。 11.適した宿主が真核細胞である請求の範囲第9項の宿主ベクター系。 12.真核細胞が昆虫細胞である請求の範囲第11項の宿主ベクター系。 13.請求の範囲第9項の宿主ベクター系をタンパク質の生産を可能にする条件 下で成長させ、それにより生産されるタンパク質を回収することを含む、SDS ポリアクリルアミドゲル電気泳動により測定される約27kDの見掛けの分子量 を有し、サイクリンE−Cdk2複合体に結 合し、その活性化を阻害することができるタンパク質の製造法。 14.(a)適した量のp27タンパク質、サイクリンE、Cdk2及び薬剤を 適した条件下で接触させ; (b)そのように接触させられたp27、サイクリンE、Cdk2及び薬 剤を、p27タンパク質の不在下では活性サイクリンE−Cdk2複合体を形成 させる条件に供し; (c)そのようにして形成される活性サイクリンE−Cdk2複合体の量 を定量的に測定し; (d)そのようにして形成される活性サイクリンE−Cdk2複合体の量 を薬剤の不在下で形成される活性サイクリンE−Cdk2複合体の量と比較する ことを含んでなり、薬剤の不在下におけるより多量の、薬剤の存在下で形成され る活性サイクリンE−Cdk2複合体が、薬剤がサイクリンE−Cdk2複合体 の活性化を阻害するp27タンパク質の能力を特異的に阻害できることを示す、 薬剤がサイクリンE−Cdk2複合体の活性化を阻害するp27タンパク質の能 力を特異的に阻害することができるか否かの決定方法。 15.(a)適した量のp27タンパク質、サイクリンE、Cdk2及び薬剤を 適した条件下で接触させ; (b)そのように接触させられたp27、サイクリンE、Cdk2及び薬 剤を、p27タンパク質の不在下では活性サイクリンE−Cdk2複合体を形成 させる条件に供し; (c)そのようにして形成される活性サイクリンE−Cdk2複合体の量 を定量的に測定し; (d)そのようにして形成される活性サイクリンE−Cdk2複 合体の量を薬剤の不在下で形成される活性サイクリンE−Cdk2複合体の量と 比較することを含んでなり、薬剤の不在下におけるより少量の、薬剤の存在下で 形成される活性サイクリンE−Cdk2複合体が、薬剤かサイクリンE−Cdk 2複合体の活性化を阻害するp27タンパク質の能力を特異的に増強できること を示す、 薬剤がサイクリンE−Cdk2複合体の活性化を阻害するp27タンパク質の能 力を特異的に増強することができるか否かの決定方法。 16.患者に治療的有効量の、患者の高増殖性細胞においてサイクリンE−Cd k2複合体の活性化を阻害するp27タンパク質の能力を特異的に増強すること ができる薬剤を投与し、それにより患者を処置することを含む、高増殖性障害を 有する患者の処置方法。 17.患者がヒトである請求の範囲第16項の方法。 18.高増殖性障害が癌及び過形成から成る群より選ばれる請求の範囲第16項 の方法。 19.患者に治療的有効量の、患者の低増殖性細胞においてサイクリンE−Cd k2複合体の活性化を阻害するp27タンパク質の能力を特異的に阻害すること ができる薬剤を投与し、それにより患者を処置することを含む、低増殖性障害を 有する患者の処置方法。 20.患者がヒトである請求の範囲第19項の方法。 21.低増殖性障害が潰瘍である請求の範囲第19項の方法。 22.患者の細胞において、高増殖性障害を伴うp27タンパク質突然変異の存 在を測定し、それにより患者における高増殖性障害を診断することを含む、患者 の細胞におけるp27タンパク質突然変異の存在に伴う、患者における高増殖性 障害の診断方法。 23.患者がヒトである請求の範囲第22項の方法。 24.高増殖性障害が癌である請求の範囲第22項の方法。 25.有効量の、適した宿主細胞に感染することができ、請求の範囲第2項の核 酸分子を含む組み換えウィルス、及び製薬学的に許容し得る担体を含む製薬学的 組成物。 26.患者の処置に有効な量の請求の範囲第25項の製薬学的組成物を患者に投 与することを含む、患者の細胞におけるp27タンパク質突然変異に伴う高増殖 性障害に苦しむ患者の処置の方法。 27.患者がヒトである請求の範囲第26項の方法。 28.高増殖性障害が癌である請求の範囲第26項の方法。
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US17904594A | 1994-01-07 | 1994-01-07 | |
US08/179,045 | 1994-01-07 | ||
US08/275,983 US5688665A (en) | 1994-01-07 | 1994-07-15 | Isolated nucleic acid molecules encoding the p27 KIP-1 protein |
US08/275,983 | 1994-07-15 | ||
PCT/US1995/000247 WO1995018824A1 (en) | 1994-01-07 | 1995-01-09 | ISOLATED p27 PROTEIN AND ITS ENCODING NUCLEIC ACID MOLECULES |
Publications (2)
Publication Number | Publication Date |
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JPH09509311A true JPH09509311A (ja) | 1997-09-22 |
JP4143119B2 JP4143119B2 (ja) | 2008-09-03 |
Family
ID=26874941
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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JP51864595A Expired - Fee Related JP4143119B2 (ja) | 1994-01-07 | 1995-01-09 | 単離p27タンパク質及びそれをコードする核酸分子 |
Country Status (8)
Country | Link |
---|---|
US (1) | US5688665A (ja) |
EP (1) | EP0749442B1 (ja) |
JP (1) | JP4143119B2 (ja) |
AT (1) | ATE271129T1 (ja) |
AU (1) | AU699969B2 (ja) |
CA (1) | CA2180570C (ja) |
DE (1) | DE69533255T2 (ja) |
WO (1) | WO1995018824A1 (ja) |
Families Citing this family (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6242575B1 (en) | 1994-01-07 | 2001-06-05 | Fred Hutchinson Institute For Cancer Research | Antibodies for detecting p27 protein |
WO1996002140A1 (en) | 1994-07-15 | 1996-02-01 | Sloan-Kettering Institute For Cancer Research | ISOLATED p27 PROTEIN AND METHODS FOR ITS PRODUCTION AND USE |
US6316208B1 (en) | 1994-01-07 | 2001-11-13 | Memorial Sloan-Kettering Cancer Center | Methods for determining isolated p27 protein levels and uses thereof |
US6355774B1 (en) | 1994-01-07 | 2002-03-12 | Fred Hutchinson Cancer Research Center | Isolated p27 protein |
AU5297496A (en) * | 1995-02-17 | 1996-09-04 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Methods of preparation and use of recombinant adenoviral vectors |
US5863904A (en) | 1995-09-26 | 1999-01-26 | The University Of Michigan | Methods for treating cancers and restenosis with P21 |
US7087582B1 (en) | 1995-09-26 | 2006-08-08 | Regents Of The University Of Michigan | Combination for site-specifically transforming cells in vivo comprising a double-balloon catheter and nucleic acid comprising a gene encoding P21 |
WO1997012900A2 (en) * | 1995-09-29 | 1997-04-10 | Ciba-Geigy Ag | Ring finger protein |
WO1997026327A1 (en) | 1996-01-18 | 1997-07-24 | Fred Hutchinson Cancer Research Center | Compositions and methods for mediating cell cycle progression |
EP1023459B1 (en) * | 1997-05-15 | 2008-08-06 | Thomas Jefferson University | Determination of cyclin-dependent kinase inhibitor p27 levels as a prognostic factor in cancer patients |
US6177272B1 (en) * | 1997-07-21 | 2001-01-23 | The Regents Of The University Of Michigan | Method for treating vascular proliferative diseases with p27 and fusions thereof |
EP1015015B1 (en) * | 1997-08-21 | 2007-05-23 | Thomas Jefferson University | pRb2/p130 PEPTIDE INHIBITORS OF cdk2 KINASE ACTIVITY |
AU1801499A (en) * | 1997-12-01 | 1999-06-16 | Sloan-Kettering Institute For Cancer Research | Uses of p27 in prostate cancer |
US6972170B1 (en) * | 1997-12-01 | 2005-12-06 | Sloan-Kettering Institute For Cancer Research | Markers for prostate cancer |
US6413974B1 (en) * | 1998-02-26 | 2002-07-02 | Aventis Pharmaceuticals Inc. | 6,9,-disubstituted 2-[trans-(4-aminocyclohexyl) amino] purines |
US6479487B1 (en) * | 1998-02-26 | 2002-11-12 | Aventis Pharmaceuticals Inc. | 6, 9-disubstituted 2-[trans-(4-aminocyclohexyl)amino] purines |
US5973119A (en) * | 1998-06-05 | 1999-10-26 | Amgen Inc. | Cyclin E genes and proteins |
JP2002517998A (ja) * | 1998-06-18 | 2002-06-25 | キュラジェン コーポレイション | p27(KIP1)のFKBP−12との相互作用 |
US6589505B1 (en) | 1999-01-29 | 2003-07-08 | St. Jude Children's Research Hospital | Cells that lack p19ink4d and p27kip1 activity and methods of use thereof |
CN100387717C (zh) | 1999-05-14 | 2008-05-14 | 弗雷德哈钦森癌症研究中心 | 通过功能性地抑制植物细胞周期蛋白抑制剂基因增加植物细胞增殖的方法 |
WO2000077258A1 (en) * | 1999-06-10 | 2000-12-21 | Sloan-Kettering Institute For Cancer Research | Markers for prostate cancer |
JP2003502065A (ja) * | 1999-06-18 | 2003-01-21 | アドヴァンスト リサーチ アンド テクノロジー インスティチュート、インコーポレイティッド | 高められた増殖能を持つ心筋細胞、およびその製造および使用法 |
US7482506B2 (en) * | 2001-03-26 | 2009-01-27 | University Of South Florida | Ras p27 mouse models and uses thereof |
GB0117600D0 (en) * | 2001-07-19 | 2001-09-12 | Trikon Holdings Ltd | Semiconductor structure |
US7601299B2 (en) * | 2004-06-18 | 2009-10-13 | Roche Diagnostics Operations, Inc. | System and method for coding information on a biosensor test strip |
US20080053911A1 (en) * | 2006-08-30 | 2008-03-06 | Qiagen Gmbh | Separation of an organic phase from a mixture comprising organic and aqueous phases by solid phase systems |
MX2016010328A (es) | 2014-02-10 | 2017-02-06 | Hutchinson Fred Cancer Res | Tratamiento con halogeno de ataque cardiaco y lesion isquemica. |
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1994
- 1994-07-15 US US08/275,983 patent/US5688665A/en not_active Expired - Lifetime
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1995
- 1995-01-09 CA CA002180570A patent/CA2180570C/en not_active Expired - Fee Related
- 1995-01-09 EP EP95906793A patent/EP0749442B1/en not_active Expired - Lifetime
- 1995-01-09 AT AT95906793T patent/ATE271129T1/de not_active IP Right Cessation
- 1995-01-09 AU AU15251/95A patent/AU699969B2/en not_active Ceased
- 1995-01-09 DE DE69533255T patent/DE69533255T2/de not_active Expired - Lifetime
- 1995-01-09 WO PCT/US1995/000247 patent/WO1995018824A1/en active IP Right Grant
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Also Published As
Publication number | Publication date |
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CA2180570C (en) | 2002-03-26 |
EP0749442A1 (en) | 1996-12-27 |
US5688665A (en) | 1997-11-18 |
CA2180570A1 (en) | 1995-07-13 |
WO1995018824A1 (en) | 1995-07-13 |
EP0749442B1 (en) | 2004-07-14 |
AU1525195A (en) | 1995-08-01 |
ATE271129T1 (de) | 2004-07-15 |
DE69533255T2 (de) | 2005-07-28 |
JP4143119B2 (ja) | 2008-09-03 |
EP0749442A4 (en) | 2000-06-28 |
AU699969B2 (en) | 1998-12-17 |
DE69533255D1 (de) | 2004-08-19 |
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