JPH08511677A - クローン化ヒトアルファ−フェトプロティンの発現及び精製 - Google Patents
クローン化ヒトアルファ−フェトプロティンの発現及び精製Info
- Publication number
- JPH08511677A JPH08511677A JP6507567A JP50756794A JPH08511677A JP H08511677 A JPH08511677 A JP H08511677A JP 6507567 A JP6507567 A JP 6507567A JP 50756794 A JP50756794 A JP 50756794A JP H08511677 A JPH08511677 A JP H08511677A
- Authority
- JP
- Japan
- Prior art keywords
- fetoprotein
- afp
- human alpha
- expression
- human
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4715—Pregnancy proteins, e.g. placenta proteins, alpha-feto-protein, pregnancy specific beta glycoprotein
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Reproductive Health (AREA)
- Biochemistry (AREA)
- Pregnancy & Childbirth (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Toxicology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gynecology & Obstetrics (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.ヒトアルファ−フェトプロティンの発現を指令することのできる発現制御因 子に作動的に結合している前記ヒトアルファ−フェトプロティンをコードする組 み換えDNA分子を含有する形質転換された原核生物細胞の提供と、前記形質転 換細胞による前記ヒトアルファ−フェトプロティンの発現可能化を含む、原核生 物細胞中におけるヒトアルファ−フェトプロティンを生産する方法。 2.前記原核生物細胞がE.coliであることを特徴とする請求項1に記載の方法。 3.前記発現制御因子がE.coliTrpプロモータであることを特徴とする請求項 1に記載の方法。 4.前記発現制御因子がE.coliTacプロモータであることを特徴とする請求項 1に記載の方法。 5.請求項1に記載の方法により生産された事実上純化されたヒトアルファ−フ ェトプロティン。 6.請求項5に記載の事実上純化されたヒトアルファ−フェトプロティンを含有 する治療用組成物。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US76743591A | 1991-09-27 | 1991-09-27 | |
PCT/US1992/008317 WO1994010199A1 (en) | 1991-09-27 | 1992-10-30 | Expression and purification of cloned human alpha-fetoprotein |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2007290274A Division JP2008120804A (ja) | 2007-11-08 | 2007-11-08 | クローン化ヒトアルファ−フェトプロティンの発現及び精製 |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH08511677A true JPH08511677A (ja) | 1996-12-10 |
Family
ID=26785055
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP6507567A Pending JPH08511677A (ja) | 1991-09-27 | 1992-10-30 | クローン化ヒトアルファ−フェトプロティンの発現及び精製 |
Country Status (8)
Country | Link |
---|---|
US (1) | US5384250A (ja) |
EP (1) | EP0749441B1 (ja) |
JP (1) | JPH08511677A (ja) |
AT (1) | ATE285416T1 (ja) |
CA (1) | CA2120131A1 (ja) |
DE (1) | DE69233463T2 (ja) |
ES (1) | ES2235156T3 (ja) |
WO (1) | WO1994010199A1 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002534077A (ja) * | 1999-01-06 | 2002-10-15 | アトランティック バイオファーマシューティカルズ インコーポレイティッド | トランスジェニック動物における分泌型ヒトアルファ−フェト蛋白質の発現 |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5965528A (en) * | 1991-09-27 | 1999-10-12 | Mcgill University | Recombinant human alph-fetoprotein as an immunosuppressive agent |
US6288034B1 (en) * | 1995-01-24 | 2001-09-11 | Martinex R & D Inc. | Recombinant human alpha-fetoprotein as an immunosuppressive agent |
US6331611B1 (en) | 1991-09-27 | 2001-12-18 | Mcgill University | Expression and purification of cloned human alpha-fetoprotein |
US5674842A (en) * | 1994-10-26 | 1997-10-07 | Health Research, Incorporated | Growth inhibitory peptide |
US7101837B1 (en) | 1995-01-24 | 2006-09-05 | Martinex R & D Inc. | Recombinant human alpha-fetoprotein as a cell proliferative agent |
CN1150030C (zh) * | 1995-01-24 | 2004-05-19 | 马蒂尼克斯研究与发展公司 | 重组人甲胎蛋白及其用途 |
US6534479B1 (en) | 1995-01-24 | 2003-03-18 | Martinex R & D Inc. | Recombinant alpha-fetoprotein hybrid cytotoxins for treating and diagnosing cancers |
US5866329A (en) * | 1995-09-27 | 1999-02-02 | Demetriou; Achilles A. | Method and probe for detection of gene associated with liver neoplastic disease |
US6087334A (en) * | 1998-08-21 | 2000-07-11 | Amylin Pharmaceuticals, Inc. | Anti-diabetic peptides |
US7208576B2 (en) * | 1999-01-06 | 2007-04-24 | Merrimack Pharmaceuticals, Inc. | Non-glycosylated human alpha-fetoprotein, methods of production, and uses thereof |
EP1417301B1 (en) * | 2001-08-07 | 2006-11-02 | Novozymes A/S | Carbohydrates and polyols for dissolving protein crystals |
EA011606B1 (ru) * | 2004-07-14 | 2009-04-28 | Эдуард Борисович Татулов | Способы получения и применения рекомбинантного альфа-фетопротеина |
CN101743018A (zh) * | 2006-12-19 | 2010-06-16 | 梅里麦克药品公司 | 甲胎蛋白和免疫调谐剂的共同施用以治疗多发性硬化 |
AU2008219007A1 (en) | 2007-02-20 | 2008-08-28 | Merrimack Pharmaceuticals, Inc. | Methods of treating multiple sclerosis by administration of alpha-fetoprotein in combination with an integrin antagonist |
US20100304873A1 (en) * | 2009-05-28 | 2010-12-02 | Lipa Markowitz | Bowling Ball and Football Game Controller |
CN105732795A (zh) * | 2016-03-21 | 2016-07-06 | 海南医学院 | 一种获得人类甲胎蛋白的方法 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH025866A (ja) * | 1988-06-27 | 1990-01-10 | Kansai Shin Gijutsu Kenkyusho:Kk | ヒトアルファフェトプロテインドメインi遺伝子、対応プラスミド組換体、対応形質転換体、該ドメインiの製造法及び製造された該ドメインi |
US5206153A (en) * | 1990-06-27 | 1993-04-27 | Snow Brand Milk Products Co., Ltd. | Method of producing human α-fetoprotein and product produced thereby |
-
1992
- 1992-09-28 CA CA002120131A patent/CA2120131A1/en not_active Abandoned
- 1992-10-30 EP EP93915109A patent/EP0749441B1/en not_active Expired - Lifetime
- 1992-10-30 AT AT93915109T patent/ATE285416T1/de not_active IP Right Cessation
- 1992-10-30 DE DE69233463T patent/DE69233463T2/de not_active Expired - Fee Related
- 1992-10-30 ES ES93915109T patent/ES2235156T3/es not_active Expired - Lifetime
- 1992-10-30 WO PCT/US1992/008317 patent/WO1994010199A1/en active IP Right Grant
- 1992-10-30 JP JP6507567A patent/JPH08511677A/ja active Pending
-
1993
- 1993-10-08 US US08/133,773 patent/US5384250A/en not_active Expired - Lifetime
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002534077A (ja) * | 1999-01-06 | 2002-10-15 | アトランティック バイオファーマシューティカルズ インコーポレイティッド | トランスジェニック動物における分泌型ヒトアルファ−フェト蛋白質の発現 |
Also Published As
Publication number | Publication date |
---|---|
DE69233463D1 (de) | 2005-01-27 |
WO1994010199A1 (en) | 1994-05-11 |
CA2120131A1 (en) | 1994-05-11 |
EP0749441B1 (en) | 2004-12-22 |
DE69233463T2 (de) | 2005-12-22 |
ATE285416T1 (de) | 2005-01-15 |
US5384250A (en) | 1995-01-24 |
ES2235156T3 (es) | 2005-07-01 |
EP0749441A1 (en) | 1996-12-27 |
EP0749441A4 (en) | 1998-12-30 |
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