JPH0742307B2 - 蛋白質の復元法 - Google Patents

蛋白質の復元法

Info

Publication number
JPH0742307B2
JPH0742307B2 JP62083132A JP8313287A JPH0742307B2 JP H0742307 B2 JPH0742307 B2 JP H0742307B2 JP 62083132 A JP62083132 A JP 62083132A JP 8313287 A JP8313287 A JP 8313287A JP H0742307 B2 JPH0742307 B2 JP H0742307B2
Authority
JP
Japan
Prior art keywords
protein
reactivation
concentration
reconstitution
buffer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP62083132A
Other languages
English (en)
Japanese (ja)
Other versions
JPS62242697A (ja
Inventor
ライナー・ルードルフ
シユテフアン・フイツシヤー
Original Assignee
ベーリンガー・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ベーリンガー・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング filed Critical ベーリンガー・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング
Publication of JPS62242697A publication Critical patent/JPS62242697A/ja
Publication of JPH0742307B2 publication Critical patent/JPH0742307B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP62083132A 1986-04-08 1987-04-06 蛋白質の復元法 Expired - Lifetime JPH0742307B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3611817.6 1986-04-08
DE19863611817 DE3611817A1 (de) 1986-04-08 1986-04-08 Verfahren zur renaturierung von proteinen

Publications (2)

Publication Number Publication Date
JPS62242697A JPS62242697A (ja) 1987-10-23
JPH0742307B2 true JPH0742307B2 (ja) 1995-05-10

Family

ID=6298240

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62083132A Expired - Lifetime JPH0742307B2 (ja) 1986-04-08 1987-04-06 蛋白質の復元法

Country Status (20)

Country Link
US (1) US4933434A (https=)
EP (1) EP0241022B1 (https=)
JP (1) JPH0742307B2 (https=)
KR (1) KR900005279B1 (https=)
AT (1) ATE84541T1 (https=)
AU (1) AU583723B2 (https=)
CA (1) CA1304191C (https=)
CZ (1) CZ283304B6 (https=)
DD (1) DD260280A5 (https=)
DE (2) DE3611817A1 (https=)
DK (1) DK173650B1 (https=)
ES (1) ES2038969T3 (https=)
FI (1) FI93842C (https=)
GR (1) GR3007605T3 (https=)
HK (1) HK46896A (https=)
HU (1) HU198514B (https=)
IE (1) IE60064B1 (https=)
IL (1) IL82091A (https=)
SK (1) SK279976B6 (https=)
ZA (1) ZA872474B (https=)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989001484A1 (fr) * 1987-08-14 1989-02-23 Gebro Broschek Kg Pharmazeutische Fabrik Procede d'oxydation intramoleculaire de deux groupes -sh dans un compose peptidique de façon a former un pont bisulfure et peptide produit selon ce procede
DE3832898A1 (de) * 1988-09-28 1990-04-12 Boehringer Mannheim Gmbh Praeparat von in prokaryonten exprimiertem plasminogenaktivator
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
DE3903581A1 (de) * 1989-02-07 1990-08-16 Boehringer Mannheim Gmbh Gewebs-plasminogenaktivator-derivat
JPH06500084A (ja) * 1990-08-20 1994-01-06 ノボ　ノルディスク　アクティーゼルスカブ 生物学的に活性な化合物、その製造方法及びその利用
AU664021B2 (en) * 1990-09-05 1995-11-02 Natinco Nv Solubilization of proteins in active forms
US5023323A (en) * 1990-09-12 1991-06-11 Monsanto Company Method of somatotropin naturation
DE4037196A1 (de) * 1990-11-22 1992-05-27 Boehringer Mannheim Gmbh Verfahren zur reaktivierung von denaturiertem protein
DE4139000A1 (de) 1991-11-27 1993-06-03 Boehringer Mannheim Gmbh Verfahren zur gentechnologischen herstellung von biologisch aktivem ss-ngf
EP1077263A1 (de) 1999-07-29 2001-02-21 F.Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen durch Co-Sekretion von Chaperonen
US20050079526A1 (en) * 2002-02-20 2005-04-14 Affinium Pharmaceuticals, Inc. Methods and apparatuses for characterizing refolding and aggregation of biological molecules
CN102892780B (zh) 2010-03-17 2015-07-29 通益制药有限公司 获得生物活性的重组人g-csf的方法
WO2012104099A1 (en) 2011-02-04 2012-08-09 Glucometrix Ag Process for the production of recombinant trypsin
CN106699836A (zh) * 2017-03-07 2017-05-24 安徽鑫华坤生物工程有限公司 一种蛋白质复性的透析设备
CN120341163B (zh) * 2025-06-20 2025-09-30 深圳市中芯辰瑞光电科技有限公司 一种固晶头

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4216141A (en) 1978-07-19 1980-08-05 The Salk Institute For Biological Studies Method for cyclization of peptides

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3231658A1 (https=) 1981-01-21 1983-11-17
IE55163B1 (en) * 1982-06-07 1990-06-20 Celltech Ltd A process for the preparation of chymosin
JPS593711U (ja) * 1982-06-30 1984-01-11 協伸工業株式会社 電線被覆剥取器
AU538170B2 (en) * 1982-09-15 1984-08-02 Owens-Illinois Glass Container Inc. Modified protein to mould an enzyme type molecule
GR79124B (https=) * 1982-12-22 1984-10-02 Genentech Inc
EP0122080B1 (en) * 1983-03-25 1989-09-13 Celltech Limited A process for the production of a protein
US4530787A (en) * 1984-03-28 1985-07-23 Cetus Corporation Controlled oxidation of microbially produced cysteine-containing proteins
KR860007688A (ko) 1985-03-08 1986-10-15 시끼 모리야 전자석장치(電磁石裝置)
GB8508340D0 (en) * 1985-03-29 1985-05-09 Creighton T E Production of protein
EP0208539A3 (en) * 1985-07-11 1988-08-03 Repligen Corporation Folding disulfide-cross-linkable proteins
US4656255A (en) * 1985-09-09 1987-04-07 International Minerals & Chemical Corp. Protein recovery
DE3618817A1 (de) * 1986-06-04 1987-12-10 Behringwerke Ag Verfahren zur gewinnung aktiver proteine aus einer biologisch inaktiven form

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4216141A (en) 1978-07-19 1980-08-05 The Salk Institute For Biological Studies Method for cyclization of peptides

Also Published As

Publication number Publication date
DD260280A5 (de) 1988-09-21
GR3007605T3 (https=) 1993-08-31
KR900005279B1 (ko) 1990-07-27
CA1304191C (en) 1992-06-23
EP0241022A3 (en) 1989-10-25
ATE84541T1 (de) 1993-01-15
CZ249587A3 (en) 1997-11-12
CZ283304B6 (cs) 1998-02-18
HK46896A (en) 1996-03-22
HU198514B (en) 1989-10-30
DE3783497D1 (de) 1993-02-25
IL82091A0 (en) 1987-10-30
IE60064B1 (en) 1994-06-01
FI871522L (fi) 1987-10-09
IL82091A (en) 1991-12-15
AU7119087A (en) 1987-10-15
SK249587A3 (en) 1999-06-11
EP0241022A2 (de) 1987-10-14
EP0241022B1 (de) 1993-01-13
IE870695L (en) 1987-10-08
ES2038969T3 (es) 1993-08-16
FI93842B (fi) 1995-02-28
US4933434A (en) 1990-06-12
JPS62242697A (ja) 1987-10-23
FI871522A0 (fi) 1987-04-07
DK173650B1 (da) 2001-05-21
HUT44046A (en) 1988-01-28
AU583723B2 (en) 1989-05-04
DK179787D0 (da) 1987-04-08
DE3611817A1 (de) 1987-10-15
SK279976B6 (sk) 1999-06-11
ZA872474B (en) 1988-07-27
FI93842C (fi) 1995-06-12
KR870010185A (ko) 1987-11-30
DK179787A (da) 1987-10-09

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