ES2038969T3 - Procedimiento para la renaturalizacion de proteinas. - Google Patents

Procedimiento para la renaturalizacion de proteinas.

Info

Publication number
ES2038969T3
ES2038969T3 ES198787105230T ES87105230T ES2038969T3 ES 2038969 T3 ES2038969 T3 ES 2038969T3 ES 198787105230 T ES198787105230 T ES 198787105230T ES 87105230 T ES87105230 T ES 87105230T ES 2038969 T3 ES2038969 T3 ES 2038969T3
Authority
ES
Spain
Prior art keywords
proteins
renaturalization
procedure
renatured
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES198787105230T
Other languages
English (en)
Inventor
Rainer Dr. Rer. Nat. Rudolph
Stephen Dr. Rer. Nat. Fischer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Application granted granted Critical
Publication of ES2038969T3 publication Critical patent/ES2038969T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PARA LA RENATURALIZACION DE PROTEINAS DESNATURALIZADAS DISUELTAS EN UN TAMPON DE RENATURALIZACION. SE PREPARA UNA DISOLUCION DE CONCENTRACION CRITICA DE LA PROTEINA EN EL TAMPON ELEGIDO, Y, UNA VEZ QUE SE FORMA EL PRODUCTO INTERMEDIO, SE AÑADE MAS PROTEINA A RENATURALIZAR EN LA CANTIDAD NECESARIA PARA ALCANZAR LA CONCENTRACION CRITICA.
ES198787105230T 1986-04-08 1987-04-08 Procedimiento para la renaturalizacion de proteinas. Expired - Lifetime ES2038969T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19863611817 DE3611817A1 (de) 1986-04-08 1986-04-08 Verfahren zur renaturierung von proteinen

Publications (1)

Publication Number Publication Date
ES2038969T3 true ES2038969T3 (es) 1993-08-16

Family

ID=6298240

Family Applications (1)

Application Number Title Priority Date Filing Date
ES198787105230T Expired - Lifetime ES2038969T3 (es) 1986-04-08 1987-04-08 Procedimiento para la renaturalizacion de proteinas.

Country Status (20)

Country Link
US (1) US4933434A (es)
EP (1) EP0241022B1 (es)
JP (1) JPH0742307B2 (es)
KR (1) KR900005279B1 (es)
AT (1) ATE84541T1 (es)
AU (1) AU583723B2 (es)
CA (1) CA1304191C (es)
CZ (1) CZ283304B6 (es)
DD (1) DD260280A5 (es)
DE (2) DE3611817A1 (es)
DK (1) DK173650B1 (es)
ES (1) ES2038969T3 (es)
FI (1) FI93842C (es)
GR (1) GR3007605T3 (es)
HK (1) HK46896A (es)
HU (1) HU198514B (es)
IE (1) IE60064B1 (es)
IL (1) IL82091A (es)
SK (1) SK279976B6 (es)
ZA (1) ZA872474B (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989001484A1 (en) * 1987-08-14 1989-02-23 Gebro Broschek Kg Pharmazeutische Fabrik Process for intramolecular oxidation of two -sh groups in a peptide compound to a disulphide bridge, and peptide so obtained
DE3832898A1 (de) * 1988-09-28 1990-04-12 Boehringer Mannheim Gmbh Praeparat von in prokaryonten exprimiertem plasminogenaktivator
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
DE3903581A1 (de) * 1989-02-07 1990-08-16 Boehringer Mannheim Gmbh Gewebs-plasminogenaktivator-derivat
ATE154073T1 (de) * 1990-08-20 1997-06-15 Novo Nordisk As Prozess für die herstellung von biologisch aktivem igf-1 durch verwendung von amino-terminal verlängertem igf-1
ES2119779T3 (es) * 1990-09-05 1998-10-16 Southern Cross Biotech Pty Ltd Solubilizacion de proteinas en formas activas.
US5023323A (en) * 1990-09-12 1991-06-11 Monsanto Company Method of somatotropin naturation
DE4037196A1 (de) * 1990-11-22 1992-05-27 Boehringer Mannheim Gmbh Verfahren zur reaktivierung von denaturiertem protein
DE4139000A1 (de) 1991-11-27 1993-06-03 Boehringer Mannheim Gmbh Verfahren zur gentechnologischen herstellung von biologisch aktivem ss-ngf
EP1077263A1 (de) 1999-07-29 2001-02-21 F.Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen durch Co-Sekretion von Chaperonen
US20050079526A1 (en) * 2002-02-20 2005-04-14 Affinium Pharmaceuticals, Inc. Methods and apparatuses for characterizing refolding and aggregation of biological molecules
CN102892780B (zh) 2010-03-17 2015-07-29 通益制药有限公司 获得生物活性的重组人g-csf的方法
WO2012104099A1 (en) 2011-02-04 2012-08-09 Glucometrix Ag Process for the production of recombinant trypsin
CN106699836A (zh) * 2017-03-07 2017-05-24 安徽鑫华坤生物工程有限公司 一种蛋白质复性的透析设备

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4216141A (en) 1978-07-19 1980-08-05 The Salk Institute For Biological Studies Method for cyclization of peptides
DE3231658A1 (es) 1981-01-21 1983-11-17
AU553017B2 (en) * 1982-06-07 1986-06-26 Chr. Hansen A/S A process for the preparation of chymosin
JPS593711U (ja) * 1982-06-30 1984-01-11 協伸工業株式会社 電線被覆剥取器
AU538170B2 (en) * 1982-09-15 1984-08-02 Owens-Illinois Glass Container Inc. Modified protein to mould an enzyme type molecule
GR79124B (es) * 1982-12-22 1984-10-02 Genentech Inc
JPH06102034B2 (ja) * 1983-03-25 1994-12-14 セルテク リミテツド タンパク質の生産方法
US4530787A (en) * 1984-03-28 1985-07-23 Cetus Corporation Controlled oxidation of microbially produced cysteine-containing proteins
KR860007688A (ko) 1985-03-08 1986-10-15 시끼 모리야 전자석장치(電磁石裝置)
GB8508340D0 (en) * 1985-03-29 1985-05-09 Creighton T E Production of protein
EP0208539A3 (en) * 1985-07-11 1988-08-03 Repligen Corporation Folding disulfide-cross-linkable proteins
US4656255A (en) * 1985-09-09 1987-04-07 International Minerals & Chemical Corp. Protein recovery
DE3618817A1 (de) * 1986-06-04 1987-12-10 Behringwerke Ag Verfahren zur gewinnung aktiver proteine aus einer biologisch inaktiven form

Also Published As

Publication number Publication date
EP0241022B1 (de) 1993-01-13
IL82091A0 (en) 1987-10-30
EP0241022A2 (de) 1987-10-14
CZ283304B6 (cs) 1998-02-18
DE3611817A1 (de) 1987-10-15
AU583723B2 (en) 1989-05-04
DK179787A (da) 1987-10-09
CA1304191C (en) 1992-06-23
HK46896A (en) 1996-03-22
JPH0742307B2 (ja) 1995-05-10
CZ249587A3 (en) 1997-11-12
JPS62242697A (ja) 1987-10-23
US4933434A (en) 1990-06-12
FI93842C (fi) 1995-06-12
HU198514B (en) 1989-10-30
ATE84541T1 (de) 1993-01-15
DK173650B1 (da) 2001-05-21
KR870010185A (ko) 1987-11-30
HUT44046A (en) 1988-01-28
DE3783497D1 (de) 1993-02-25
ZA872474B (en) 1988-07-27
IE60064B1 (en) 1994-06-01
AU7119087A (en) 1987-10-15
IE870695L (en) 1987-10-08
DD260280A5 (de) 1988-09-21
FI93842B (fi) 1995-02-28
FI871522A0 (fi) 1987-04-07
SK249587A3 (en) 1999-06-11
GR3007605T3 (es) 1993-08-31
DK179787D0 (da) 1987-04-08
FI871522A (fi) 1987-10-09
SK279976B6 (sk) 1999-06-11
IL82091A (en) 1991-12-15
KR900005279B1 (ko) 1990-07-27
EP0241022A3 (en) 1989-10-25

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