JPH07108232B2 - ペプチド又は蛋白質の製造方法 - Google Patents

ペプチド又は蛋白質の製造方法

Info

Publication number
JPH07108232B2
JPH07108232B2 JP2271880A JP27188090A JPH07108232B2 JP H07108232 B2 JPH07108232 B2 JP H07108232B2 JP 2271880 A JP2271880 A JP 2271880A JP 27188090 A JP27188090 A JP 27188090A JP H07108232 B2 JPH07108232 B2 JP H07108232B2
Authority
JP
Japan
Prior art keywords
protein
peptide
protease
arg
dipeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP2271880A
Other languages
English (en)
Japanese (ja)
Other versions
JPH04148694A (ja
Inventor
浩明 山本
邦彦 山下
Original Assignee
エム・ディ・リサーチ株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by エム・ディ・リサーチ株式会社 filed Critical エム・ディ・リサーチ株式会社
Priority to JP2271880A priority Critical patent/JPH07108232B2/ja
Priority to CA002070781A priority patent/CA2070781A1/en
Priority to PCT/JP1991/000239 priority patent/WO1992006211A1/ja
Priority to EP91904654A priority patent/EP0591524A1/en
Publication of JPH04148694A publication Critical patent/JPH04148694A/ja
Priority to US08/243,082 priority patent/US5506120A/en
Publication of JPH07108232B2 publication Critical patent/JPH07108232B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/57563—Vasoactive intestinal peptide [VIP]; Related peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Vascular Medicine (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
JP2271880A 1990-10-09 1990-10-09 ペプチド又は蛋白質の製造方法 Expired - Lifetime JPH07108232B2 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2271880A JPH07108232B2 (ja) 1990-10-09 1990-10-09 ペプチド又は蛋白質の製造方法
CA002070781A CA2070781A1 (en) 1990-10-09 1991-02-25 Method of producing peptides or proteins
PCT/JP1991/000239 WO1992006211A1 (en) 1990-10-09 1991-02-25 Process for producing peptide or protein
EP91904654A EP0591524A1 (en) 1990-10-09 1991-02-25 Process for producing peptide or protein
US08/243,082 US5506120A (en) 1990-10-09 1994-05-16 Method of producing peptides or proteins as fusion proteins

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2271880A JPH07108232B2 (ja) 1990-10-09 1990-10-09 ペプチド又は蛋白質の製造方法

Publications (2)

Publication Number Publication Date
JPH04148694A JPH04148694A (ja) 1992-05-21
JPH07108232B2 true JPH07108232B2 (ja) 1995-11-22

Family

ID=17506194

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2271880A Expired - Lifetime JPH07108232B2 (ja) 1990-10-09 1990-10-09 ペプチド又は蛋白質の製造方法

Country Status (5)

Country Link
US (1) US5506120A (https=)
EP (1) EP0591524A1 (https=)
JP (1) JPH07108232B2 (https=)
CA (1) CA2070781A1 (https=)
WO (1) WO1992006211A1 (https=)

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US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
EP0548267A4 (en) * 1990-09-04 1994-11-23 Salk Inst Biotech Ind Production of insulin-like growth factor-1 in methylotrophic yeast cells
ATE220105T1 (de) * 1991-04-01 2002-07-15 Merck & Co Inc Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung
FR2686899B1 (fr) 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
FR2701953B1 (fr) * 1993-02-22 1995-05-24 Centre Nat Rech Scient Protéine de fusion multi-VIP et procédé de préparation de VIP recombinant.
US6316004B1 (en) * 1993-06-22 2001-11-13 T. Tikhonenko Chimeric somatostatin containing protein and encoding DNA, plasmids of expression, method for preparing chimeric protein, strain-producers, immunogenic composition, method for increasing the productivity of farm animals
US5888775A (en) * 1994-04-29 1999-03-30 Dade International Inc Peptide synthesis and purification by fusion to penI protein or precipitation effective portion thereof
US6037145A (en) * 1994-09-07 2000-03-14 Suntory Limited Process for production of protein
BR9608278A (pt) * 1995-04-11 1999-10-13 Merck & Co Inc Processo para produzir um intermediário de dipeptìdeo de um composto à base de dipeptìdeo
US6037321A (en) * 1995-05-03 2000-03-14 Biostar Inc. Fusion proteins comprising vasoactive intestinal peptide or PACAP
WO1996034958A1 (en) * 1995-05-03 1996-11-07 Biostar Inc. Vasoactive intestinal peptide
WO1997001627A1 (en) * 1995-06-27 1997-01-16 Igen International, Inc. High-level expression and efficient recovery of ubiquitin fusion proteins from escherichia coli
GB9513967D0 (en) * 1995-07-08 1995-09-06 Univ Leicester Insulin
US20080286228A1 (en) * 1995-10-20 2008-11-20 Tarantolo Stefano R Compositions and methods for enhancing immune responses mediated by antigen-presenting cells
JP4125784B2 (ja) * 1995-10-20 2008-07-30 ユニバーシティ・オブ・ネブラスカ・ボード・オブ・リージェンツ 抗原提示細胞により仲介される免疫応答を高めるための組成物および方法
GB9526733D0 (en) 1995-12-30 1996-02-28 Delta Biotechnology Ltd Fusion proteins
US5935824A (en) * 1996-01-31 1999-08-10 Technologene, Inc. Protein expression system
CA2198966C (en) * 1996-03-04 2011-06-21 Yuji Suzuki Method for cleaving chimeric protein using processing enzyme
US6265204B1 (en) 1997-01-17 2001-07-24 Genencor International, Inc. DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi
GB9716790D0 (en) 1997-08-07 1997-10-15 Creative Peptides Sweden Ab Recombinant DNA molecules comprising multimeric copies of a gene sequence and expression thereof
KR100368073B1 (ko) * 1999-03-09 2003-01-24 한국생명공학연구원 융합파트너를 이용한 재조합 단백질의 제조방법
EP2206720A1 (en) 2000-04-12 2010-07-14 Human Genome Sciences, Inc. Albumin fusion proteins
US7507413B2 (en) 2001-04-12 2009-03-24 Human Genome Sciences, Inc. Albumin fusion proteins
AU2002364587A1 (en) 2001-12-21 2003-07-30 Human Genome Sciences, Inc. Albumin fusion proteins
EP2261250B1 (en) 2001-12-21 2015-07-01 Human Genome Sciences, Inc. GCSF-Albumin fusion proteins
US20080194481A1 (en) 2001-12-21 2008-08-14 Human Genome Sciences, Inc. Albumin Fusion Proteins
ATE443767T1 (de) * 2002-04-22 2009-10-15 Dow Global Technologies Inc Kostengünstige herstellung von peptiden
DE60334109D1 (de) * 2002-06-29 2010-10-21 Leadbio Inc Yapsin-mangelmutantenstamm von hansenula polymorpha sowie verfahren zur herstellung rekombinanter proteine damit
BRPI0406647A (pt) * 2003-01-06 2005-12-06 Angiochem Inc Método para transportar um composto através da barreira sanguìnea do cérebro
CA2513213C (en) 2003-01-22 2013-07-30 Human Genome Sciences, Inc. Albumin fusion proteins
AU2004276687B2 (en) * 2003-09-30 2011-07-14 Asubio Pharma Co., Ltd. Method of cleaving polypeptide by using OmpT protease mutant
ES2567634T3 (es) * 2004-02-09 2016-04-25 Human Genome Sciences, Inc. Proteínas de fusión de albúmina
RU2369404C2 (ru) * 2004-02-09 2009-10-10 Хьюман Дженом Сайенсиз, Инк. Слитые белки альбумина
US20130172274A1 (en) 2005-12-20 2013-07-04 Duke University Methods and compositions for delivering active agents with enhanced pharmacological properties
US8334365B2 (en) 2006-06-07 2012-12-18 Human Genome Sciences, Inc. Albumin fusion proteins
US9365634B2 (en) * 2007-05-29 2016-06-14 Angiochem Inc. Aprotinin-like polypeptides for delivering agents conjugated thereto to tissues
EP3412300A1 (en) 2008-06-27 2018-12-12 Duke University Therapeutic agents comprising elastin-like peptides
BRPI0920209A2 (pt) 2008-10-15 2015-12-22 Angiochem Inc conjugados de agonistas de glp-1 e usos dos mesmos
DK3311828T3 (da) * 2009-08-14 2021-06-28 Phasebio Pharmaceuticals Inc Modificerede vasoaktive tarmpeptider
WO2012170524A1 (en) 2011-06-06 2012-12-13 Phasebio Pharmaceuticals, Inc. Use of modified vasoactive intestinal peptides in the treatment of hypertension
WO2015172046A1 (en) 2014-05-08 2015-11-12 Phasebio Pharmaceuticals, Inc. Methods and compositions for treating cystic fibrosis
CA2976038A1 (en) 2015-02-09 2016-08-18 Phasebio Pharmaceuticals, Inc. Methods and compositions for treating muscle disease and disorders
DK3307326T3 (da) 2015-06-15 2020-10-19 Angiochem Inc Fremgangsmåder til behandling af leptomeningeal karcinomatose
US20200024321A1 (en) 2017-03-20 2020-01-23 Lupin Limited Expression and large-scale production of peptides

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CA1200773A (en) * 1980-02-29 1986-02-18 William J. Rutter Expression linkers
SE8300693L (sv) * 1983-02-09 1984-08-10 Sven Lofdahl Sett att framstella och isolera proteiner och polypeptider samt en hybridvektor for detta
US4703004A (en) * 1984-01-24 1987-10-27 Immunex Corporation Synthesis of protein with an identification peptide
CA1213537A (en) * 1984-05-01 1986-11-04 Canadian Patents And Development Limited - Societe Canadienne Des Brevets Et D'exploitation Limitee Polypeptide expression method
US4828994A (en) * 1984-09-21 1989-05-09 Genex Corporation Bacillus strains with reduced extracellular protease levels
DE3679343D1 (de) * 1985-03-28 1991-06-27 Chiron Corp Expression durch verwendung von fusionsgenen fuer proteinproduktion.
JP2862870B2 (ja) * 1986-09-12 1999-03-03 協和醗酵工業株式会社 新規ペプチド
EP0312346B1 (en) * 1987-10-16 1994-08-17 Genencor International, Inc. E. coli sequence specific acid protease
JPH01191683A (ja) * 1988-01-27 1989-08-01 M D Res Kk 新規なプロテアーゼ
JPH0249585A (ja) * 1988-08-12 1990-02-19 M D Res Kk 新規なプロテアーゼ
DE68922014T2 (de) * 1988-01-27 1995-08-03 M & D Research Co Protease.
JP2643968B2 (ja) * 1988-02-03 1997-08-25 サントリー株式会社 Ｋｅｘ２エンドプロテアーゼ及びその製造方法
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Ｊ．Ｂａｃｔｅｒｉｏｌ．，Ｖｏｌ．１７０，Ｐ．５６２５−５６３２（１９８８）
Ｊ．Ｂｉｏｌ．Ｃｈｅｍ．，Ｖｏｌ．２６２，Ｎｏ．２０，Ｐ．９６１５−９６２０（１９８７）

Also Published As

Publication number Publication date
US5506120A (en) 1996-04-09
JPH04148694A (ja) 1992-05-21
WO1992006211A1 (en) 1992-04-16
EP0591524A4 (https=) 1994-08-03
EP0591524A1 (en) 1994-04-13
CA2070781A1 (en) 1992-04-10

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