JPH06509226A - 組み替え蛋白質の製造法及び用いられる宿主細胞 - Google Patents
組み替え蛋白質の製造法及び用いられる宿主細胞Info
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- JPH06509226A JPH06509226A JP5502002A JP50200293A JPH06509226A JP H06509226 A JPH06509226 A JP H06509226A JP 5502002 A JP5502002 A JP 5502002A JP 50200293 A JP50200293 A JP 50200293A JP H06509226 A JPH06509226 A JP H06509226A
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- 239000000284 extract Substances 0.000 description 1
- 229960004222 factor ix Drugs 0.000 description 1
- 229940012413 factor vii Drugs 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000012215 gene cloning Methods 0.000 description 1
- 239000006481 glucose medium Substances 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- XIXADJRWDQXREU-UHFFFAOYSA-M lithium acetate Chemical compound [Li+].CC([O-])=O XIXADJRWDQXREU-UHFFFAOYSA-M 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000003471 mutagenic agent Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 101150015886 nuc-1 gene Proteins 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000003016 pheromone Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 230000004481 post-translational protein modification Effects 0.000 description 1
- 230000001124 posttranscriptional effect Effects 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000000892 thaumatin Substances 0.000 description 1
- 235000010436 thaumatin Nutrition 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/76—Albumins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR91/08217 | 1991-07-02 | ||
| FR9108217A FR2678636A1 (fr) | 1991-07-02 | 1991-07-02 | Procede de production de proteines recombinantes et cellules hote utilisees. |
| PCT/FR1992/000610 WO1993001274A1 (fr) | 1991-07-02 | 1992-06-30 | Procede de production de proteines recombinantes et cellules hotes utilisees |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH06509226A true JPH06509226A (ja) | 1994-10-20 |
Family
ID=9414582
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5502002A Pending JPH06509226A (ja) | 1991-07-02 | 1992-06-30 | 組み替え蛋白質の製造法及び用いられる宿主細胞 |
Country Status (14)
| Country | Link |
|---|---|
| EP (2) | EP0521767A1 (cg-RX-API-DMAC7.html) |
| JP (1) | JPH06509226A (cg-RX-API-DMAC7.html) |
| AT (1) | ATE250126T1 (cg-RX-API-DMAC7.html) |
| AU (1) | AU2278392A (cg-RX-API-DMAC7.html) |
| CA (1) | CA2110992A1 (cg-RX-API-DMAC7.html) |
| DE (1) | DE69233206T2 (cg-RX-API-DMAC7.html) |
| DK (1) | DK0592574T3 (cg-RX-API-DMAC7.html) |
| ES (1) | ES2203611T3 (cg-RX-API-DMAC7.html) |
| FR (1) | FR2678636A1 (cg-RX-API-DMAC7.html) |
| IE (1) | IE922148A1 (cg-RX-API-DMAC7.html) |
| NO (1) | NO934572L (cg-RX-API-DMAC7.html) |
| NZ (1) | NZ243403A (cg-RX-API-DMAC7.html) |
| PT (1) | PT592574E (cg-RX-API-DMAC7.html) |
| WO (1) | WO1993001274A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105063080A (zh) * | 2015-09-07 | 2015-11-18 | 复旦大学 | 不含有信号肽的在马克斯克鲁维酵母营养缺陷型菌株中进行外源基因表达的重组载体 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3381090D1 (de) * | 1982-05-19 | 1990-02-15 | Gist Brocades Nv | Klonierungssystem fuer kluyveromyce spezies. |
| IT1203758B (it) * | 1986-03-27 | 1989-02-23 | Univ Roma | Vettori di clonazione e di espressione di geni eterologhi in lieviti e lieviti trasformati con tali vettori |
| AU628018B2 (en) * | 1987-07-28 | 1992-09-10 | Dsm Ip Assets B.V. | Kluyveromyces as a host strain |
| FR2649991B2 (fr) * | 1988-08-05 | 1994-03-04 | Rhone Poulenc Sante | Utilisation de derives stables du plasmide pkd1 pour l'expression et la secretion de proteines heterologues dans les levures du genre kluyveromyces |
-
1991
- 1991-07-02 FR FR9108217A patent/FR2678636A1/fr active Granted
-
1992
- 1992-06-30 PT PT92915114T patent/PT592574E/pt unknown
- 1992-06-30 EP EP92401848A patent/EP0521767A1/fr active Pending
- 1992-06-30 EP EP92915114A patent/EP0592574B1/fr not_active Expired - Lifetime
- 1992-06-30 ES ES92915114T patent/ES2203611T3/es not_active Expired - Lifetime
- 1992-06-30 JP JP5502002A patent/JPH06509226A/ja active Pending
- 1992-06-30 CA CA002110992A patent/CA2110992A1/fr not_active Abandoned
- 1992-06-30 DE DE69233206T patent/DE69233206T2/de not_active Expired - Lifetime
- 1992-06-30 DK DK92915114T patent/DK0592574T3/da active
- 1992-06-30 AT AT92915114T patent/ATE250126T1/de active
- 1992-06-30 WO PCT/FR1992/000610 patent/WO1993001274A1/fr not_active Ceased
- 1992-06-30 AU AU22783/92A patent/AU2278392A/en not_active Abandoned
- 1992-07-01 NZ NZ243403A patent/NZ243403A/en unknown
- 1992-07-01 IE IE214892A patent/IE922148A1/en not_active Application Discontinuation
-
1993
- 1993-12-13 NO NO934572A patent/NO934572L/no unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PT592574E (pt) | 2004-02-27 |
| NO934572D0 (no) | 1993-12-13 |
| DE69233206D1 (de) | 2003-10-23 |
| FR2678636B1 (cg-RX-API-DMAC7.html) | 1994-08-19 |
| NO934572L (no) | 1993-12-13 |
| ES2203611T3 (es) | 2004-04-16 |
| NZ243403A (en) | 1994-12-22 |
| CA2110992A1 (fr) | 1993-01-21 |
| EP0592574B1 (fr) | 2003-09-17 |
| EP0521767A1 (fr) | 1993-01-07 |
| DK0592574T3 (da) | 2004-01-19 |
| EP0592574A1 (fr) | 1994-04-20 |
| ATE250126T1 (de) | 2003-10-15 |
| DE69233206T2 (de) | 2004-07-01 |
| FR2678636A1 (fr) | 1993-01-08 |
| AU2278392A (en) | 1993-02-11 |
| WO1993001274A1 (fr) | 1993-01-21 |
| IE922148A1 (en) | 1993-01-13 |
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