ES2203611T3 - Procedimiento de proteccion de proteinas recombinantes y celulas hospedantes utilizadas. - Google Patents
Procedimiento de proteccion de proteinas recombinantes y celulas hospedantes utilizadas.Info
- Publication number
- ES2203611T3 ES2203611T3 ES92915114T ES92915114T ES2203611T3 ES 2203611 T3 ES2203611 T3 ES 2203611T3 ES 92915114 T ES92915114 T ES 92915114T ES 92915114 T ES92915114 T ES 92915114T ES 2203611 T3 ES2203611 T3 ES 2203611T3
- Authority
- ES
- Spain
- Prior art keywords
- host cell
- cell according
- protein
- chosen
- lactis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- 230000037431 insertion Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 239000000138 intercalating agent Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- XIXADJRWDQXREU-UHFFFAOYSA-M lithium acetate Chemical compound [Li+].CC([O-])=O XIXADJRWDQXREU-UHFFFAOYSA-M 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000003471 mutagenic agent Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000003016 pheromone Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 230000001124 posttranscriptional effect Effects 0.000 description 1
- 230000001323 posttranslational effect Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000005026 transcription initiation Effects 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 1
- 229940045145 uridine Drugs 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/76—Albumins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9108217 | 1991-07-02 | ||
| FR9108217A FR2678636A1 (fr) | 1991-07-02 | 1991-07-02 | Procede de production de proteines recombinantes et cellules hote utilisees. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2203611T3 true ES2203611T3 (es) | 2004-04-16 |
Family
ID=9414582
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES92915114T Expired - Lifetime ES2203611T3 (es) | 1991-07-02 | 1992-06-30 | Procedimiento de proteccion de proteinas recombinantes y celulas hospedantes utilizadas. |
Country Status (14)
| Country | Link |
|---|---|
| EP (2) | EP0521767A1 (cg-RX-API-DMAC7.html) |
| JP (1) | JPH06509226A (cg-RX-API-DMAC7.html) |
| AT (1) | ATE250126T1 (cg-RX-API-DMAC7.html) |
| AU (1) | AU2278392A (cg-RX-API-DMAC7.html) |
| CA (1) | CA2110992A1 (cg-RX-API-DMAC7.html) |
| DE (1) | DE69233206T2 (cg-RX-API-DMAC7.html) |
| DK (1) | DK0592574T3 (cg-RX-API-DMAC7.html) |
| ES (1) | ES2203611T3 (cg-RX-API-DMAC7.html) |
| FR (1) | FR2678636A1 (cg-RX-API-DMAC7.html) |
| IE (1) | IE922148A1 (cg-RX-API-DMAC7.html) |
| NO (1) | NO934572L (cg-RX-API-DMAC7.html) |
| NZ (1) | NZ243403A (cg-RX-API-DMAC7.html) |
| PT (1) | PT592574E (cg-RX-API-DMAC7.html) |
| WO (1) | WO1993001274A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105063080A (zh) * | 2015-09-07 | 2015-11-18 | 复旦大学 | 不含有信号肽的在马克斯克鲁维酵母营养缺陷型菌株中进行外源基因表达的重组载体 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3381090D1 (de) * | 1982-05-19 | 1990-02-15 | Gist Brocades Nv | Klonierungssystem fuer kluyveromyce spezies. |
| IT1203758B (it) * | 1986-03-27 | 1989-02-23 | Univ Roma | Vettori di clonazione e di espressione di geni eterologhi in lieviti e lieviti trasformati con tali vettori |
| AU628018B2 (en) * | 1987-07-28 | 1992-09-10 | Dsm Ip Assets B.V. | Kluyveromyces as a host strain |
| FR2649991B2 (fr) * | 1988-08-05 | 1994-03-04 | Rhone Poulenc Sante | Utilisation de derives stables du plasmide pkd1 pour l'expression et la secretion de proteines heterologues dans les levures du genre kluyveromyces |
-
1991
- 1991-07-02 FR FR9108217A patent/FR2678636A1/fr active Granted
-
1992
- 1992-06-30 PT PT92915114T patent/PT592574E/pt unknown
- 1992-06-30 EP EP92401848A patent/EP0521767A1/fr active Pending
- 1992-06-30 EP EP92915114A patent/EP0592574B1/fr not_active Expired - Lifetime
- 1992-06-30 ES ES92915114T patent/ES2203611T3/es not_active Expired - Lifetime
- 1992-06-30 JP JP5502002A patent/JPH06509226A/ja active Pending
- 1992-06-30 CA CA002110992A patent/CA2110992A1/fr not_active Abandoned
- 1992-06-30 DE DE69233206T patent/DE69233206T2/de not_active Expired - Lifetime
- 1992-06-30 DK DK92915114T patent/DK0592574T3/da active
- 1992-06-30 AT AT92915114T patent/ATE250126T1/de active
- 1992-06-30 WO PCT/FR1992/000610 patent/WO1993001274A1/fr not_active Ceased
- 1992-06-30 AU AU22783/92A patent/AU2278392A/en not_active Abandoned
- 1992-07-01 NZ NZ243403A patent/NZ243403A/en unknown
- 1992-07-01 IE IE214892A patent/IE922148A1/en not_active Application Discontinuation
-
1993
- 1993-12-13 NO NO934572A patent/NO934572L/no unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PT592574E (pt) | 2004-02-27 |
| NO934572D0 (no) | 1993-12-13 |
| DE69233206D1 (de) | 2003-10-23 |
| JPH06509226A (ja) | 1994-10-20 |
| FR2678636B1 (cg-RX-API-DMAC7.html) | 1994-08-19 |
| NO934572L (no) | 1993-12-13 |
| NZ243403A (en) | 1994-12-22 |
| CA2110992A1 (fr) | 1993-01-21 |
| EP0592574B1 (fr) | 2003-09-17 |
| EP0521767A1 (fr) | 1993-01-07 |
| DK0592574T3 (da) | 2004-01-19 |
| EP0592574A1 (fr) | 1994-04-20 |
| ATE250126T1 (de) | 2003-10-15 |
| DE69233206T2 (de) | 2004-07-01 |
| FR2678636A1 (fr) | 1993-01-08 |
| AU2278392A (en) | 1993-02-11 |
| WO1993001274A1 (fr) | 1993-01-21 |
| IE922148A1 (en) | 1993-01-13 |
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