JPH06506700A - 鋳型依存性酵素的核酸合成用プライマーとしてのオリゴヌクレオチド同時合成および直接標識化のための機能性担体 - Google Patents
鋳型依存性酵素的核酸合成用プライマーとしてのオリゴヌクレオチド同時合成および直接標識化のための機能性担体Info
- Publication number
- JPH06506700A JPH06506700A JP5504888A JP50488893A JPH06506700A JP H06506700 A JPH06506700 A JP H06506700A JP 5504888 A JP5504888 A JP 5504888A JP 50488893 A JP50488893 A JP 50488893A JP H06506700 A JPH06506700 A JP H06506700A
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- JP
- Japan
- Prior art keywords
- labeling
- labeled
- synthesis
- oligonucleotides
- reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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- 238000002474 experimental method Methods 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
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- 230000005484 gravity Effects 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- BHEPBYXIRTUNPN-UHFFFAOYSA-N hydridophosphorus(.) (triplet) Chemical compound [PH] BHEPBYXIRTUNPN-UHFFFAOYSA-N 0.000 description 1
- 150000003949 imides Chemical class 0.000 description 1
- 229910000032 lithium hydrogen carbonate Inorganic materials 0.000 description 1
- HQRPHMAXFVUBJX-UHFFFAOYSA-M lithium;hydrogen carbonate Chemical compound [Li+].OC([O-])=O HQRPHMAXFVUBJX-UHFFFAOYSA-M 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- OKDQKPLMQBXTNH-UHFFFAOYSA-N n,n-dimethyl-2h-pyridin-1-amine Chemical compound CN(C)N1CC=CC=C1 OKDQKPLMQBXTNH-UHFFFAOYSA-N 0.000 description 1
- LVTXQKPWSPEYGL-BFHYXJOUSA-N n-[9-[(2r,4s,5r)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]purin-6-yl]-4-methylbenzenesulfonamide Chemical compound C1=CC(C)=CC=C1S(=O)(=O)NC1=NC=NC2=C1N=CN2[C@@H]1O[C@H](CO)[C@@H](O)C1 LVTXQKPWSPEYGL-BFHYXJOUSA-N 0.000 description 1
- 125000001280 n-hexyl group Chemical group C(CCCCC)* 0.000 description 1
- 235000019645 odor Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 125000006239 protecting group Chemical group 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 239000012925 reference material Substances 0.000 description 1
- 125000006853 reporter group Chemical group 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000004007 reversed phase HPLC Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 238000007086 side reaction Methods 0.000 description 1
- 238000010898 silica gel chromatography Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 238000010532 solid phase synthesis reaction Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000001384 succinic acid Substances 0.000 description 1
- 125000000472 sulfonyl group Chemical group *S(*)(=O)=O 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 125000002221 trityl group Chemical group [H]C1=C([H])C([H])=C([H])C([H])=C1C([*])(C1=C(C(=C(C(=C1[H])[H])[H])[H])[H])C1=C([H])C([H])=C([H])C([H])=C1[H] 0.000 description 1
- 238000005866 tritylation reaction Methods 0.000 description 1
- 238000002525 ultrasonication Methods 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
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- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (10)
- 1.標識化基を与えられたヌクレオシドまたはヌクレオチドまたはその誘導体も しくは前駆体がアンカー基を介して共有結合されることを特徴とする高分子担体 。
- 2.ヌクレオシド、ヌクレオシドまたはその誘導体もしくは前駆体がその3′− ヒドロキシル基を介して相のアンカー基に結合される請求項1記載の担体。
- 3.標識化基が核塩基に結合される請求項1記載の担体。
- 4.3′末端ヌクレオチド標識オリゴヌクレオチドの合成のための請求項1記載 の高分子担体の使用。
- 5.3′末端に所定の数および型のデオキシヌクレオシドまたはりボヌクレオシ ド、デオキシ−またはリボヌクレオチドまたはその誘導体もしくは前駆体を有す ることを特徴とする、少なくとも1つの標識化基を含有するオリゴヌクレオチド 。
- 6.DNAまたはRNAポリメラーゼによって触媒される鋳型依存性反応におけ るプライマーとしての請求項5記載のオリゴヌクレオチドの使用。
- 7.反応が酵素的核酸配列決定法の一部である請求項6記載の使用。
- 8.反応がポリメラーゼ連鎖反応の一部である請求項6記載の使用。
- 9.プライマーがその3′末端に標識化基を与えるヌクレオシドを含有すること を特徴とする、プライマーの鋳型核酸ヘのハイブリダイゼーシヨン、次いで、そ の3′末端で開始されるプライマーの酵素的伸長による標識核酸の製造方法。
- 10.請求項1記載の担体がさらなるモノヌクレオシド単位との結合によって延 長されることを特徴とする、高分子担体上での化学的合成による末端ヌクレオチ ド標識オリゴヌクレオチドの製造方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE4128480 | 1991-08-28 | ||
DE4128480,1 | 1991-08-28 | ||
PCT/EP1992/001932 WO1993005060A1 (de) | 1991-08-28 | 1992-08-22 | Funktionalisierte trägermaterialien für gleichzeitige synthese und direktmarkierung von oligonukleotiden als primer für matrizenabhängige enzymatische nukleinsäuresynthesen |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH06506700A true JPH06506700A (ja) | 1994-07-28 |
JP2562862B2 JP2562862B2 (ja) | 1996-12-11 |
Family
ID=6439283
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP5504888A Expired - Lifetime JP2562862B2 (ja) | 1991-08-28 | 1992-08-22 | 鋳型依存性酵素的核酸合成用プライマーとしてのオリゴヌクレオチド同時合成および直接標識化のための機能性担体 |
Country Status (10)
Country | Link |
---|---|
US (1) | US5574141A (ja) |
EP (1) | EP0600965B1 (ja) |
JP (1) | JP2562862B2 (ja) |
KR (1) | KR0123559B1 (ja) |
AT (1) | ATE163308T1 (ja) |
CA (1) | CA2113478C (ja) |
DE (2) | DE4227882A1 (ja) |
DK (1) | DK0600965T3 (ja) |
ES (1) | ES2116342T3 (ja) |
WO (1) | WO1993005060A1 (ja) |
Families Citing this family (20)
Publication number | Priority date | Publication date | Assignee | Title |
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DE4418691A1 (de) * | 1994-05-28 | 1996-02-22 | Boehringer Mannheim Gmbh | 3'-(4'-) nicht-radioaktiv markierte Nukleoside und Nukleotide mit Aminocarbonsäure-, Peptid- oder Carbonsäure-Spacer |
CA2279669A1 (en) * | 1995-12-15 | 1997-06-16 | Enzo Therapeutics, Inc. | Property effecting and/or property exhibiting constructs for the expression of non-native nucleic acid processing components for therapeutic and diagnostic uses |
US6291164B1 (en) | 1996-11-22 | 2001-09-18 | Invitrogen Corporation | Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate |
US6235483B1 (en) * | 2000-01-31 | 2001-05-22 | Agilent Technologies, Inc. | Methods and kits for indirect labeling of nucleic acids |
JP4128453B2 (ja) * | 2001-03-19 | 2008-07-30 | プレジデント アンド フェロウズ オブ ハーバード カレッジ | 新規分子機能の進化 |
WO2002103008A2 (en) | 2001-06-20 | 2002-12-27 | Nuevolution A/S | Templated molecules and methods for using such molecules |
EP1540013B1 (en) * | 2002-08-19 | 2015-07-08 | The President and Fellows of Harvard College | Evolving new molecular function |
US8017323B2 (en) * | 2003-03-26 | 2011-09-13 | President And Fellows Of Harvard College | Free reactant use in nucleic acid-templated synthesis |
WO2007011722A2 (en) | 2005-07-15 | 2007-01-25 | President And Fellows Of Harvard College | Reaction discovery system |
SG10201408401RA (en) | 2009-09-16 | 2015-01-29 | Genentech Inc | Coiled coil and/or tether containing protein complexes and uses thereof |
CN102782134B (zh) | 2009-12-29 | 2017-11-24 | 库尔纳公司 | 通过抑制核呼吸因子1(nrf1)的天然反义转录物而治疗nrf1相关疾病 |
AR080793A1 (es) | 2010-03-26 | 2012-05-09 | Roche Glycart Ag | Anticuerpos biespecificos |
EP2655414B1 (en) | 2010-12-23 | 2018-08-29 | Roche Diagniostics GmbH | Bispecific binding agent |
JP6046049B2 (ja) | 2010-12-23 | 2016-12-14 | エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft | 二価結合剤による翻訳後修飾されたポリペプチドの検出 |
JP5766296B2 (ja) | 2010-12-23 | 2015-08-19 | エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft | ポリペプチド−ポリヌクレオチド複合体、およびエフェクター成分の標的化された送達におけるその使用 |
EP2812357B1 (en) | 2012-02-10 | 2020-11-04 | F.Hoffmann-La Roche Ag | Single-chain antibodies and other heteromultimers |
MX354862B (es) | 2012-06-27 | 2018-03-23 | Hoffmann La Roche | Método para la producción de entidades dirigidas altamente selectivas hechas a la medida y biespecíficas que contienen dos entidades de unión diferentes. |
CA2871882A1 (en) | 2012-06-27 | 2014-01-03 | F. Hoffmann-La Roche Ag | Method for making antibody fc-region conjugates comprising at least one binding entity that specifically binds to a target and uses thereof |
WO2014021938A1 (en) * | 2012-08-02 | 2014-02-06 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and apparatus for nucleic acid synthesis using oligo-templated polymerization |
CN107001482B (zh) | 2014-12-03 | 2021-06-15 | 豪夫迈·罗氏有限公司 | 多特异性抗体 |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
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SU666183A1 (ru) * | 1977-06-15 | 1979-06-05 | Новосибирский институт органической химии СО АН СССР | Способ получени активных производных динуклеотидов |
FR2519005B1 (fr) * | 1981-12-29 | 1985-10-25 | Pasteur Institut | Fragments d'adn marques a l'une au moins de leurs extremites par des ribonucleotides modifies reconnaissables par des molecules affines et procede pour realiser une analyse de tels fragments d'adn |
DK171161B1 (da) * | 1985-03-28 | 1996-07-08 | Hoffmann La Roche | Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve |
US4739044A (en) * | 1985-06-13 | 1988-04-19 | Amgen | Method for derivitization of polynucleotides |
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SU1318600A1 (ru) * | 1985-10-09 | 1987-06-23 | Всесоюзный Научно-Исследовательский Институт Молекулярной Биологии | Способ твердофазного синтеза олигонуклеотидов |
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SU1439108A1 (ru) * | 1986-05-11 | 1988-11-23 | Всесоюзный Научно-Исследовательский Институт Молекулярной Биологии | Способ получени носителей дл твердофазного синтеза олигонуклеотидов |
DE3801987A1 (de) * | 1988-01-23 | 1989-07-27 | Boehringer Mannheim Gmbh | Traeger zur chemischen oder/und enzymatischen umsetzung von nukleinsaeuren oder nukleinsaeurefragmenten an festphasen |
US5082934A (en) * | 1989-04-05 | 1992-01-21 | Naxcor | Coumarin derivatives for use as nucleotide crosslinking reagents |
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-
1992
- 1992-08-22 AT AT92917597T patent/ATE163308T1/de not_active IP Right Cessation
- 1992-08-22 DK DK92917597T patent/DK0600965T3/da active
- 1992-08-22 WO PCT/EP1992/001932 patent/WO1993005060A1/de active IP Right Grant
- 1992-08-22 DE DE4227882A patent/DE4227882A1/de not_active Withdrawn
- 1992-08-22 US US08/199,248 patent/US5574141A/en not_active Expired - Lifetime
- 1992-08-22 JP JP5504888A patent/JP2562862B2/ja not_active Expired - Lifetime
- 1992-08-22 ES ES92917597T patent/ES2116342T3/es not_active Expired - Lifetime
- 1992-08-22 EP EP92917597A patent/EP0600965B1/de not_active Expired - Lifetime
- 1992-08-22 DE DE59209203T patent/DE59209203D1/de not_active Expired - Lifetime
- 1992-08-22 KR KR1019940700659A patent/KR0123559B1/ko not_active IP Right Cessation
- 1992-08-22 CA CA002113478A patent/CA2113478C/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
KR0123559B1 (ko) | 1997-11-28 |
DE4227882A1 (de) | 1993-04-01 |
US5574141A (en) | 1996-11-12 |
EP0600965B1 (de) | 1998-02-18 |
DK0600965T3 (da) | 1998-09-23 |
JP2562862B2 (ja) | 1996-12-11 |
ATE163308T1 (de) | 1998-03-15 |
CA2113478C (en) | 2001-06-12 |
CA2113478A1 (en) | 1993-03-18 |
EP0600965A1 (de) | 1994-06-15 |
ES2116342T3 (es) | 1998-07-16 |
DE59209203D1 (de) | 1998-03-26 |
WO1993005060A1 (de) | 1993-03-18 |
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