ES2116342T3 - Cebador para sintesis enzimaticas de acidos nucleicos dependientes de la matriz. - Google Patents
Cebador para sintesis enzimaticas de acidos nucleicos dependientes de la matriz.Info
- Publication number
- ES2116342T3 ES2116342T3 ES92917597T ES92917597T ES2116342T3 ES 2116342 T3 ES2116342 T3 ES 2116342T3 ES 92917597 T ES92917597 T ES 92917597T ES 92917597 T ES92917597 T ES 92917597T ES 2116342 T3 ES2116342 T3 ES 2116342T3
- Authority
- ES
- Spain
- Prior art keywords
- pct
- primer
- matrix
- nucleic acids
- sec
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
Abstract
SE DESCRIBE LA SINTESIS Y LA UTILIZACION DE SOPORTES POLIMEROS CARGADOS CON BASES DE ACIDO NUCLEICO, QUE CONTIENEN POR SU PARTE GRUPOS DE MARCADO O SUS ETAPAS PRECURSORAS. LOS SOPORTES POLIMEROS ASI CARGADOS PUEDEN SER UTILIZADOS COMO UNA FASE LIQUIDA O SOLIDA PARA EL MONTAJE DE OLIGONUCLEOTIDOS UTILIZADOS COMO AGENTE PARA LA SINTESIS DE ACIDOS NUCLEICOS ENZIMATICOS DEPENDIENTES DE MATRICES, POR EJEMPLO PARA EL ANALISIS SECUENCIAL DE ACUERDO CON SANGER Y COLABORADORES O PARA LA REACCION (PCR) EN CADENA POLIMERASA.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE4128480 | 1991-08-28 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2116342T3 true ES2116342T3 (es) | 1998-07-16 |
Family
ID=6439283
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES92917597T Expired - Lifetime ES2116342T3 (es) | 1991-08-28 | 1992-08-22 | Cebador para sintesis enzimaticas de acidos nucleicos dependientes de la matriz. |
Country Status (10)
Country | Link |
---|---|
US (1) | US5574141A (es) |
EP (1) | EP0600965B1 (es) |
JP (1) | JP2562862B2 (es) |
KR (1) | KR0123559B1 (es) |
AT (1) | ATE163308T1 (es) |
CA (1) | CA2113478C (es) |
DE (2) | DE59209203D1 (es) |
DK (1) | DK0600965T3 (es) |
ES (1) | ES2116342T3 (es) |
WO (1) | WO1993005060A1 (es) |
Families Citing this family (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4418691A1 (de) * | 1994-05-28 | 1996-02-22 | Boehringer Mannheim Gmbh | 3'-(4'-) nicht-radioaktiv markierte Nukleoside und Nukleotide mit Aminocarbonsäure-, Peptid- oder Carbonsäure-Spacer |
CA2279673A1 (en) * | 1995-12-15 | 1997-06-16 | Enzo Therapeutics, Inc. | Property effecting and/or property exhibiting constructs for the expression of non-native nucleic acids for therapeutic and diagnostic uses |
US6291164B1 (en) | 1996-11-22 | 2001-09-18 | Invitrogen Corporation | Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate |
US6235483B1 (en) * | 2000-01-31 | 2001-05-22 | Agilent Technologies, Inc. | Methods and kits for indirect labeling of nucleic acids |
IL158000A0 (en) * | 2001-03-19 | 2004-03-28 | Harvard College | Evolving new molecular function |
IL159268A0 (en) | 2001-06-20 | 2004-06-01 | Nuevolution As | Templated molecules and methods for using such molecules |
WO2004016767A2 (en) * | 2002-08-19 | 2004-02-26 | The President And Fellows Of Harvard College | Evolving new molecular function |
US8017323B2 (en) * | 2003-03-26 | 2011-09-13 | President And Fellows Of Harvard College | Free reactant use in nucleic acid-templated synthesis |
WO2007011722A2 (en) | 2005-07-15 | 2007-01-25 | President And Fellows Of Harvard College | Reaction discovery system |
NZ701769A (en) | 2009-09-16 | 2016-06-24 | Genentech Inc | Coiled coil and/or tether containing protein complexes and uses thereof |
RU2615450C2 (ru) | 2009-12-29 | 2017-04-04 | Курна, Инк. | Лечение заболеваний, связанных с ядерным респираторным фактором 1(nrf1), путем ингибирования природного антисмыслового транскрипта к nrf1 |
TW201138821A (en) | 2010-03-26 | 2011-11-16 | Roche Glycart Ag | Bispecific antibodies |
EP2655413B1 (en) | 2010-12-23 | 2019-01-16 | F.Hoffmann-La Roche Ag | Polypeptide-polynucleotide-complex and its use in targeted effector moiety delivery |
WO2012085113A1 (en) | 2010-12-23 | 2012-06-28 | Roche Diagnostics Gmbh | Binding agent |
ES2605493T3 (es) | 2010-12-23 | 2017-03-14 | F. Hoffmann-La Roche Ag | Detección de un polipéptido modificado post-traduccionalmente mediante un agente ligante bivalente |
MX2014009565A (es) | 2012-02-10 | 2014-11-10 | Genentech Inc | Anticuerpos monocatenarios y otros heteromultimeros. |
RU2015100656A (ru) | 2012-06-27 | 2016-08-20 | Ф. Хоффманн-Ля Рош Аг | Способ получения конъюгатов fc-фрагмента антитела, включающих по меньшей мере одну связывающую группировку, которая специфически связывается с мишенью, и их применения |
KR20150023889A (ko) | 2012-06-27 | 2015-03-05 | 에프. 호프만-라 로슈 아게 | 2 개 이상의 상이한 결합 단위를 함유하는 맞춤-제작된 고도로 선별적이고 다중-특이적인 표적화 단위의 선별 및 제조 방법 및 이의 용도 |
WO2014021938A1 (en) * | 2012-08-02 | 2014-02-06 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and apparatus for nucleic acid synthesis using oligo-templated polymerization |
WO2016087416A1 (en) | 2014-12-03 | 2016-06-09 | F. Hoffmann-La Roche Ag | Multispecific antibodies |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SU666183A1 (ru) * | 1977-06-15 | 1979-06-05 | Новосибирский институт органической химии СО АН СССР | Способ получени активных производных динуклеотидов |
FR2519005B1 (fr) * | 1981-12-29 | 1985-10-25 | Pasteur Institut | Fragments d'adn marques a l'une au moins de leurs extremites par des ribonucleotides modifies reconnaissables par des molecules affines et procede pour realiser une analyse de tels fragments d'adn |
ES8706823A1 (es) * | 1985-03-28 | 1987-06-16 | Cetus Corp | Un procedimiento para detectar la presencia o ausencia de al menos una secuencia especifica de acidos nucleicos en una muestra |
US4762779A (en) * | 1985-06-13 | 1988-08-09 | Amgen Inc. | Compositions and methods for functionalizing nucleic acids |
US4739044A (en) * | 1985-06-13 | 1988-04-19 | Amgen | Method for derivitization of polynucleotides |
SU1318600A1 (ru) * | 1985-10-09 | 1987-06-23 | Всесоюзный Научно-Исследовательский Институт Молекулярной Биологии | Способ твердофазного синтеза олигонуклеотидов |
US4868105A (en) * | 1985-12-11 | 1989-09-19 | Chiron Corporation | Solution phase nucleic acid sandwich assay |
SU1439108A1 (ru) * | 1986-05-11 | 1988-11-23 | Всесоюзный Научно-Исследовательский Институт Молекулярной Биологии | Способ получени носителей дл твердофазного синтеза олигонуклеотидов |
DE3801987A1 (de) * | 1988-01-23 | 1989-07-27 | Boehringer Mannheim Gmbh | Traeger zur chemischen oder/und enzymatischen umsetzung von nukleinsaeuren oder nukleinsaeurefragmenten an festphasen |
US5082934A (en) * | 1989-04-05 | 1992-01-21 | Naxcor | Coumarin derivatives for use as nucleotide crosslinking reagents |
US5141813A (en) * | 1989-08-28 | 1992-08-25 | Clontech Laboratories, Inc. | Multifunctional controlled pore glass reagent for solid phase oligonucleotide synthesis |
US5403709A (en) * | 1992-10-06 | 1995-04-04 | Hybridon, Inc. | Method for sequencing synthetic oligonucleotides containing non-phosphodiester internucleotide linkages |
-
1992
- 1992-08-22 EP EP92917597A patent/EP0600965B1/de not_active Expired - Lifetime
- 1992-08-22 CA CA002113478A patent/CA2113478C/en not_active Expired - Lifetime
- 1992-08-22 US US08/199,248 patent/US5574141A/en not_active Expired - Lifetime
- 1992-08-22 JP JP5504888A patent/JP2562862B2/ja not_active Expired - Lifetime
- 1992-08-22 DE DE59209203T patent/DE59209203D1/de not_active Expired - Lifetime
- 1992-08-22 DK DK92917597T patent/DK0600965T3/da active
- 1992-08-22 AT AT92917597T patent/ATE163308T1/de not_active IP Right Cessation
- 1992-08-22 KR KR1019940700659A patent/KR0123559B1/ko not_active IP Right Cessation
- 1992-08-22 ES ES92917597T patent/ES2116342T3/es not_active Expired - Lifetime
- 1992-08-22 WO PCT/EP1992/001932 patent/WO1993005060A1/de active IP Right Grant
- 1992-08-22 DE DE4227882A patent/DE4227882A1/de not_active Withdrawn
Also Published As
Publication number | Publication date |
---|---|
JPH06506700A (ja) | 1994-07-28 |
DE59209203D1 (de) | 1998-03-26 |
EP0600965A1 (de) | 1994-06-15 |
CA2113478A1 (en) | 1993-03-18 |
DE4227882A1 (de) | 1993-04-01 |
KR0123559B1 (ko) | 1997-11-28 |
ATE163308T1 (de) | 1998-03-15 |
JP2562862B2 (ja) | 1996-12-11 |
WO1993005060A1 (de) | 1993-03-18 |
CA2113478C (en) | 2001-06-12 |
US5574141A (en) | 1996-11-12 |
DK0600965T3 (da) | 1998-09-23 |
EP0600965B1 (de) | 1998-02-18 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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