JPH0516838B2 - - Google Patents
Info
- Publication number
- JPH0516838B2 JPH0516838B2 JP61225059A JP22505986A JPH0516838B2 JP H0516838 B2 JPH0516838 B2 JP H0516838B2 JP 61225059 A JP61225059 A JP 61225059A JP 22505986 A JP22505986 A JP 22505986A JP H0516838 B2 JPH0516838 B2 JP H0516838B2
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- immobilized
- rna
- nylon
- solid support
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 102000039446 nucleic acids Human genes 0.000 claims description 57
- 108020004707 nucleic acids Proteins 0.000 claims description 57
- 150000007523 nucleic acids Chemical class 0.000 claims description 57
- 239000011324 bead Substances 0.000 claims description 50
- 239000000523 sample Substances 0.000 claims description 38
- 239000004677 Nylon Substances 0.000 claims description 36
- 229920001778 nylon Polymers 0.000 claims description 36
- 238000000034 method Methods 0.000 claims description 34
- 230000027455 binding Effects 0.000 claims description 22
- 238000006243 chemical reaction Methods 0.000 claims description 18
- 239000003153 chemical reaction reagent Substances 0.000 claims description 18
- 239000007787 solid Substances 0.000 claims description 16
- 150000001412 amines Chemical class 0.000 claims description 14
- 125000003368 amide group Chemical group 0.000 claims description 13
- 108091033319 polynucleotide Proteins 0.000 claims description 11
- 102000040430 polynucleotide Human genes 0.000 claims description 11
- 239000002157 polynucleotide Substances 0.000 claims description 11
- 125000000320 amidine group Chemical group 0.000 claims description 9
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 8
- 102000004190 Enzymes Human genes 0.000 claims description 8
- 108090000790 Enzymes Proteins 0.000 claims description 8
- 150000004985 diamines Chemical class 0.000 claims description 7
- 239000003446 ligand Substances 0.000 claims description 6
- 230000000295 complement effect Effects 0.000 claims description 5
- 230000009870 specific binding Effects 0.000 claims description 5
- 108090001008 Avidin Proteins 0.000 claims description 4
- 229960002685 biotin Drugs 0.000 claims description 4
- 235000020958 biotin Nutrition 0.000 claims description 4
- 239000011616 biotin Substances 0.000 claims description 4
- 230000003100 immobilizing effect Effects 0.000 claims description 4
- 230000004913 activation Effects 0.000 claims description 3
- 125000002947 alkylene group Chemical group 0.000 claims description 3
- 125000004432 carbon atom Chemical group C* 0.000 claims description 3
- 238000002372 labelling Methods 0.000 claims description 3
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 3
- 125000000843 phenylene group Chemical group C1(=C(C=CC=C1)*)* 0.000 claims description 2
- 230000003172 anti-dna Effects 0.000 claims 1
- 125000004836 hexamethylene group Chemical group [H]C([H])([*:2])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[*:1] 0.000 claims 1
- 108020004414 DNA Proteins 0.000 description 33
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- 239000012528 membrane Substances 0.000 description 23
- 238000009396 hybridization Methods 0.000 description 21
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- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 16
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 15
- 239000000243 solution Substances 0.000 description 14
- 102000004169 proteins and genes Human genes 0.000 description 13
- 108090000623 proteins and genes Proteins 0.000 description 13
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 10
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- 239000001301 oxygen Substances 0.000 description 10
- 229910052760 oxygen Inorganic materials 0.000 description 10
- 229920000136 polysorbate Polymers 0.000 description 10
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 9
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- -1 quaternary ammonium ions Chemical class 0.000 description 9
- 150000003839 salts Chemical class 0.000 description 9
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 8
- 238000011534 incubation Methods 0.000 description 8
- 239000011780 sodium chloride Substances 0.000 description 8
- 239000012064 sodium phosphate buffer Substances 0.000 description 8
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 7
- 239000007995 HEPES buffer Substances 0.000 description 7
- 229910019142 PO4 Inorganic materials 0.000 description 7
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 7
- 239000010452 phosphate Substances 0.000 description 7
- 208000037065 Subacute sclerosing leukoencephalitis Diseases 0.000 description 6
- 206010042297 Subacute sclerosing panencephalitis Diseases 0.000 description 6
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 6
- 239000011541 reaction mixture Substances 0.000 description 6
- ZRALSGWEFCBTJO-UHFFFAOYSA-N anhydrous guanidine Natural products NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 5
- 125000002091 cationic group Chemical group 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 102000014914 Carrier Proteins Human genes 0.000 description 4
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- 108010090804 Streptavidin Proteins 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- ZYGHJZDHTFUPRJ-UHFFFAOYSA-N coumarin Chemical compound C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 description 4
- 229910001629 magnesium chloride Inorganic materials 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 241000972773 Aulopiformes Species 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 3
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 3
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 3
- 239000000020 Nitrocellulose Substances 0.000 description 3
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 3
- 239000007983 Tris buffer Substances 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 239000002168 alkylating agent Substances 0.000 description 3
- 229940100198 alkylating agent Drugs 0.000 description 3
- 125000003277 amino group Chemical group 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 239000003125 aqueous solvent Substances 0.000 description 3
- 108091008324 binding proteins Proteins 0.000 description 3
- 229940098773 bovine serum albumin Drugs 0.000 description 3
- 238000004587 chromatography analysis Methods 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
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- 239000008101 lactose Substances 0.000 description 3
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- 229920001220 nitrocellulos Polymers 0.000 description 3
- 239000002773 nucleotide Substances 0.000 description 3
- 125000003729 nucleotide group Chemical group 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
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- 235000019515 salmon Nutrition 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
- YGTHBVOCTPFJHQ-UHFFFAOYSA-N 4-(dimethylamino)butanamide Chemical compound CN(C)CCCC(N)=O YGTHBVOCTPFJHQ-UHFFFAOYSA-N 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 description 2
- 102000002464 Galactosidases Human genes 0.000 description 2
- 108010093031 Galactosidases Proteins 0.000 description 2
- 108010024636 Glutathione Proteins 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 2
- PEEHTFAAVSWFBL-UHFFFAOYSA-N Maleimide Chemical compound O=C1NC(=O)C=C1 PEEHTFAAVSWFBL-UHFFFAOYSA-N 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
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- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- 108020004682 Single-Stranded DNA Proteins 0.000 description 2
- DRTQHJPVMGBUCF-XVFCMESISA-N Uridine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-XVFCMESISA-N 0.000 description 2
- 125000000217 alkyl group Chemical group 0.000 description 2
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- 125000000129 anionic group Chemical group 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
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- VHRGRCVQAFMJIZ-UHFFFAOYSA-N cadaverine Chemical compound NCCCCCN VHRGRCVQAFMJIZ-UHFFFAOYSA-N 0.000 description 2
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- 229960000956 coumarin Drugs 0.000 description 2
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- 229920000140 heteropolymer Polymers 0.000 description 2
- 210000004408 hybridoma Anatomy 0.000 description 2
- IXCSERBJSXMMFS-UHFFFAOYSA-N hydrogen chloride Substances Cl.Cl IXCSERBJSXMMFS-UHFFFAOYSA-N 0.000 description 2
- 229910000041 hydrogen chloride Inorganic materials 0.000 description 2
- 230000003053 immunization Effects 0.000 description 2
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- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
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- KIDHWZJUCRJVML-UHFFFAOYSA-N putrescine Chemical compound NCCCCN KIDHWZJUCRJVML-UHFFFAOYSA-N 0.000 description 2
- 125000000467 secondary amino group Chemical group [H]N([*:1])[*:2] 0.000 description 2
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- 239000001488 sodium phosphate Substances 0.000 description 2
- 229910000162 sodium phosphate Inorganic materials 0.000 description 2
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 2
- 239000007790 solid phase Substances 0.000 description 2
- ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 description 2
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- NZNMSOFKMUBTKW-UHFFFAOYSA-N cyclohexanecarboxylic acid Chemical compound OC(=O)C1CCCCC1 NZNMSOFKMUBTKW-UHFFFAOYSA-N 0.000 description 1
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- UQGFMSUEHSUPRD-UHFFFAOYSA-N disodium;3,7-dioxido-2,4,6,8,9-pentaoxa-1,3,5,7-tetraborabicyclo[3.3.1]nonane Chemical compound [Na+].[Na+].O1B([O-])OB2OB([O-])OB1O2 UQGFMSUEHSUPRD-UHFFFAOYSA-N 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- CEIPQQODRKXDSB-UHFFFAOYSA-N ethyl 3-(6-hydroxynaphthalen-2-yl)-1H-indazole-5-carboximidate dihydrochloride Chemical compound Cl.Cl.C1=C(O)C=CC2=CC(C3=NNC4=CC=C(C=C43)C(=N)OCC)=CC=C21 CEIPQQODRKXDSB-UHFFFAOYSA-N 0.000 description 1
- 230000000763 evoking effect Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000012847 fine chemical Substances 0.000 description 1
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- 125000000524 functional group Chemical group 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- RPLXGDGIXIJNQD-UHFFFAOYSA-N hexane-1,3-diamine Chemical compound CCCC(N)CCN RPLXGDGIXIJNQD-UHFFFAOYSA-N 0.000 description 1
- NAQMVNRVTILPCV-UHFFFAOYSA-N hexane-1,6-diamine Chemical compound NCCCCCCN NAQMVNRVTILPCV-UHFFFAOYSA-N 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 230000002163 immunogen Effects 0.000 description 1
- 239000003547 immunosorbent Substances 0.000 description 1
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- 239000002523 lectin Substances 0.000 description 1
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- 239000003550 marker Substances 0.000 description 1
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- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
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- 244000005700 microbiome Species 0.000 description 1
- 201000000050 myeloid neoplasm Diseases 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
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- 230000001717 pathogenic effect Effects 0.000 description 1
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical class OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 229920000768 polyamine Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 150000003139 primary aliphatic amines Chemical class 0.000 description 1
- 150000003142 primary aromatic amines Chemical class 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 150000005619 secondary aliphatic amines Chemical class 0.000 description 1
- 150000003336 secondary aromatic amines Chemical class 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 235000010265 sodium sulphite Nutrition 0.000 description 1
- 229940063673 spermidine Drugs 0.000 description 1
- 229940063675 spermine Drugs 0.000 description 1
- 210000004989 spleen cell Anatomy 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 229960002175 thyroglobulin Drugs 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- DWCSXQCXXITVKE-UHFFFAOYSA-N triethyloxidanium Chemical class CC[O+](CC)CC DWCSXQCXXITVKE-UHFFFAOYSA-N 0.000 description 1
- ZSDSQXJSNMTJDA-UHFFFAOYSA-N trifluralin Chemical compound CCCN(CCC)C1=C([N+]([O-])=O)C=C(C(F)(F)F)C=C1[N+]([O-])=O ZSDSQXJSNMTJDA-UHFFFAOYSA-N 0.000 description 1
- QDNCLIPKBNMUPP-UHFFFAOYSA-N trimethyloxidanium Chemical class C[O+](C)C QDNCLIPKBNMUPP-UHFFFAOYSA-N 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 1
- 229940045145 uridine Drugs 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
- G01N33/545—Synthetic resin
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/803—Physical recovery methods, e.g. chromatography, grinding
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US781332 | 1985-09-30 | ||
| US06/781,332 US4806546A (en) | 1985-09-30 | 1985-09-30 | Immobilization of nucleic acids on derivatized nylon supports |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6281565A JPS6281565A (ja) | 1987-04-15 |
| JPH0516838B2 true JPH0516838B2 (enExample) | 1993-03-05 |
Family
ID=25122380
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61225059A Granted JPS6281565A (ja) | 1985-09-30 | 1986-09-25 | 核酸を変性ナイロン支持体に固定化する方法、これにより固定化された核酸及び試料中のポリヌクレオチド配列を測定する方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US4806546A (enExample) |
| EP (1) | EP0221308B1 (enExample) |
| JP (1) | JPS6281565A (enExample) |
| CA (1) | CA1288709C (enExample) |
| DE (1) | DE3666707D1 (enExample) |
Families Citing this family (77)
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| WO1988006633A1 (en) * | 1987-03-02 | 1988-09-07 | Arnold Lyle John Jr | Polycationic supports for nucleic acid purification, separation and hybridization |
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| CA1325761C (en) * | 1987-12-25 | 1994-01-04 | Takanori Oka | Method of detecting an intended nucleic acid in a sample |
| US4952519A (en) * | 1988-05-02 | 1990-08-28 | E. I. Du Pont De Nemours And Company | Protein immobilization with poly(ethyleneimine) derivatized with a hydroprobic group |
| JP2667447B2 (ja) * | 1988-06-28 | 1997-10-27 | オリンパス光学工業株式会社 | 固体表面への細胞の固定化方法 |
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| JP2739068B2 (ja) * | 1988-09-24 | 1998-04-08 | 春馬 川口 | Ｄｎａ固定ミクロスフェア及びそれを用いるｄｎａ転写制御因子精製法 |
| US6447804B1 (en) | 1988-10-05 | 2002-09-10 | Whatman, Plc | Dry solid medium for storage and analysis of genetic material |
| US5985327A (en) | 1988-10-05 | 1999-11-16 | Flinders Technologies Pty. Ltd. | Solid medium and method for DNA storage |
| WO1990003959A1 (en) * | 1988-10-05 | 1990-04-19 | The Flinders University Of South Australia | Solid medium and method for dna storage |
| US5972386A (en) * | 1995-12-19 | 1999-10-26 | Flinders Technologies Pty, Ltd. | Dry solid medium for storage and analysis of genetic material |
| US5496562A (en) * | 1988-10-05 | 1996-03-05 | Flinders Technologies Pty Ltd | Solid medium and method for DNA storage |
| US6627226B2 (en) | 1988-10-05 | 2003-09-30 | Whatman, Inc. | Dry solid medium for storage and analysis of genetic material |
| US5756126A (en) * | 1991-05-29 | 1998-05-26 | Flinders Technologies Pty. Ltd. | Dry solid medium for storage and analysis of genetic material |
| US5807527A (en) * | 1991-05-29 | 1998-09-15 | Flinders Technologies Pty. Ltd. | Solid medium and method for DNA storage |
| US5683916A (en) * | 1988-10-31 | 1997-11-04 | Hemasure Inc. | Membrane affinity apparatus and purification methods related thereto |
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| US5030561A (en) * | 1988-12-27 | 1991-07-09 | Becton, Dickinson And Company | Chlamydia assay using amidine modified supports or particles |
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| EP0456765A1 (en) * | 1989-01-31 | 1991-11-21 | E.I. Du Pont De Nemours And Company | Porous substrates with a high concentration of amine groups |
| US5352578A (en) * | 1989-02-15 | 1994-10-04 | Worcester Foundation For Experimental Biology | Method of separating oligonucleotides from a mixture |
| US5559221A (en) * | 1989-02-15 | 1996-09-24 | Worcester Foundation For Experimental Biology | Method of separating oligonucleotides from a mixture |
| DE69032847T2 (de) * | 1989-06-30 | 1999-05-12 | Chiron Corp., Emeryville, Calif. | Hydrophobe Nukleinsäure-Sonde |
| JPH03257369A (ja) * | 1990-02-27 | 1991-11-15 | Yon Rin Mo | 簡単なイムノドットニトロセルロースまたはナイロンペーパー |
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| US5667976A (en) * | 1990-05-11 | 1997-09-16 | Becton Dickinson And Company | Solid supports for nucleic acid hybridization assays |
| CA2095611A1 (en) * | 1990-11-14 | 1992-05-15 | Jennifer Kyoko Ishii | Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor |
| EP0834576B1 (en) * | 1990-12-06 | 2002-01-16 | Affymetrix, Inc. (a Delaware Corporation) | Detection of nucleic acid sequences |
| FR2679255B1 (fr) * | 1991-07-17 | 1993-10-22 | Bio Merieux | Procede d'immobilisation d'un fragment nucleique par fixation passive sur un support solide, support solide ainsi obtenu et son utilisation. |
| GB9208548D0 (en) * | 1992-04-21 | 1992-06-03 | Kodak Ltd | An immunoassay and test kit |
| US5795714A (en) * | 1992-11-06 | 1998-08-18 | Trustees Of Boston University | Method for replicating an array of nucleic acid probes |
| US6436635B1 (en) * | 1992-11-06 | 2002-08-20 | Boston University | Solid phase sequencing of double-stranded nucleic acids |
| US5605798A (en) | 1993-01-07 | 1997-02-25 | Sequenom, Inc. | DNA diagnostic based on mass spectrometry |
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| US6893816B1 (en) * | 1993-10-28 | 2005-05-17 | Houston Advanced Research Center | Microfabricated, flowthrough porous apparatus for discrete detection of binding reactions |
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| US7803529B1 (en) * | 1995-04-11 | 2010-09-28 | Sequenom, Inc. | Solid phase sequencing of biopolymers |
| US20060063193A1 (en) * | 1995-04-11 | 2006-03-23 | Dong-Jing Fu | Solid phase sequencing of double-stranded nucleic acids |
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| JP3523188B2 (ja) * | 2000-12-13 | 2004-04-26 | 富士写真フイルム株式会社 | プローブ分子が固定された検出具の水性処理液 |
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| WO2005102526A1 (en) | 2004-04-09 | 2005-11-03 | Research Think Tank, Inc. | Devices and methods for collection, storage and transportation of biological specimens |
| JP2009520979A (ja) * | 2005-12-21 | 2009-05-28 | コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ | 生体分子のためのセンサ及び該センサを使用した分析方法 |
| CA2667992A1 (en) * | 2006-10-31 | 2008-05-08 | Sru Biosystems, Inc. | Method for blocking non-specific protein binding on a functionalized surface |
| US20090137024A1 (en) * | 2007-11-28 | 2009-05-28 | Canon U.S. Life Sciences, Inc. | Method of Separating Target DNA from Mixed DNA |
| WO2024092388A1 (zh) * | 2022-10-31 | 2024-05-10 | 深圳华大智造科技股份有限公司 | 一种核酸加载试剂及用其检测测序芯片吸附能力的方法 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1550819A (en) * | 1975-06-04 | 1979-08-22 | Nat Res Dev | Polymeric support for biogically active materials |
| FR2422956A1 (fr) * | 1978-04-13 | 1979-11-09 | Pasteur Institut | Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede |
| JPS5510590A (en) * | 1978-05-04 | 1980-01-25 | Wellcome Found | Enzyme immunity quantity analysis |
| US4455370A (en) * | 1982-05-17 | 1984-06-19 | E. I. Du Pont De Nemours And Company | Transferring separated components in gel electrophoresis via nylon membrane |
| US4588682A (en) * | 1982-12-13 | 1986-05-13 | Integrated Genetics, Inc. | Binding nucleic acid to a support |
| US4512896A (en) * | 1983-02-07 | 1985-04-23 | Yale University | Transfer of macromolecules from a chromatographic substrate to an immobilizing matrix |
| IL71715A0 (en) * | 1983-08-05 | 1984-09-30 | Miles Lab | Method,gene probes and test kit for detecting bacteria by nucleic acid hybridization |
| US4652517A (en) * | 1984-06-11 | 1987-03-24 | Diagnostic Research Limited Partnership | Methods for the in vitro detection and identification of unknown pathogens or genetic entities |
| FR2565996B1 (fr) * | 1984-06-15 | 1988-01-22 | Inst Nat Sante Rech Med | Acides nucleiques marques chimiquement, leur utilisation et necessaire pour sa mise en oeuvre |
| US4563417A (en) * | 1984-08-31 | 1986-01-07 | Miles Laboratories, Inc. | Nucleic acid hybridization assay employing antibodies to intercalation complexes |
-
1985
- 1985-09-30 US US06/781,332 patent/US4806546A/en not_active Expired - Fee Related
-
1986
- 1986-06-13 CA CA000511592A patent/CA1288709C/en not_active Expired - Lifetime
- 1986-09-18 EP EP86112900A patent/EP0221308B1/en not_active Expired
- 1986-09-18 DE DE8686112900T patent/DE3666707D1/de not_active Expired
- 1986-09-25 JP JP61225059A patent/JPS6281565A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| US4806546A (en) | 1989-02-21 |
| EP0221308A1 (en) | 1987-05-13 |
| JPS6281565A (ja) | 1987-04-15 |
| EP0221308B1 (en) | 1989-11-02 |
| CA1288709C (en) | 1991-09-10 |
| DE3666707D1 (en) | 1989-12-07 |
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