CA1288709C - Immobilization of nucleic acids on derivatized nylon supports - Google Patents
Immobilization of nucleic acids on derivatized nylon supportsInfo
- Publication number
- CA1288709C CA1288709C CA000511592A CA511592A CA1288709C CA 1288709 C CA1288709 C CA 1288709C CA 000511592 A CA000511592 A CA 000511592A CA 511592 A CA511592 A CA 511592A CA 1288709 C CA1288709 C CA 1288709C
- Authority
- CA
- Canada
- Prior art keywords
- nucleic acid
- immobilized
- nylon
- rna
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 58
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 58
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- 239000004677 Nylon Substances 0.000 title claims abstract description 40
- 239000000523 sample Substances 0.000 claims abstract description 42
- 239000007787 solid Substances 0.000 claims abstract description 16
- 125000003368 amide group Chemical group 0.000 claims abstract description 14
- 150000001412 amines Chemical class 0.000 claims abstract description 14
- 108091033319 polynucleotide Proteins 0.000 claims abstract description 12
- 102000040430 polynucleotide Human genes 0.000 claims abstract description 12
- 239000002157 polynucleotide Substances 0.000 claims abstract description 12
- 238000006243 chemical reaction Methods 0.000 claims abstract description 11
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- ZRALSGWEFCBTJO-UHFFFAOYSA-N anhydrous guanidine Natural products NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 claims description 7
- 150000004985 diamines Chemical class 0.000 claims description 7
- 125000000320 amidine group Chemical group 0.000 claims description 6
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- 125000004836 hexamethylene group Chemical group [H]C([H])([*:2])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[*:1] 0.000 claims 1
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- 201000000050 myeloid neoplasm Diseases 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
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- 239000004033 plastic Substances 0.000 description 1
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- 150000003141 primary amines Chemical class 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 150000005619 secondary aliphatic amines Chemical class 0.000 description 1
- 150000003335 secondary amines Chemical group 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 229940083575 sodium dodecyl sulfate Drugs 0.000 description 1
- 235000010265 sodium sulphite Nutrition 0.000 description 1
- 229940063673 spermidine Drugs 0.000 description 1
- 229940063675 spermine Drugs 0.000 description 1
- 210000004989 spleen cell Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-L sulfite Chemical compound [O-]S([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-L 0.000 description 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 1
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- DWCSXQCXXITVKE-UHFFFAOYSA-N triethyloxidanium Chemical class CC[O+](CC)CC DWCSXQCXXITVKE-UHFFFAOYSA-N 0.000 description 1
- QDNCLIPKBNMUPP-UHFFFAOYSA-N trimethyloxidanium Chemical compound C[O+](C)C QDNCLIPKBNMUPP-UHFFFAOYSA-N 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
- G01N33/545—Synthetic resin
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/803—Physical recovery methods, e.g. chromatography, grinding
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US06/781,332 US4806546A (en) | 1985-09-30 | 1985-09-30 | Immobilization of nucleic acids on derivatized nylon supports |
| US781,332 | 1985-09-30 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1288709C true CA1288709C (en) | 1991-09-10 |
Family
ID=25122380
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000511592A Expired - Lifetime CA1288709C (en) | 1985-09-30 | 1986-06-13 | Immobilization of nucleic acids on derivatized nylon supports |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US4806546A (enExample) |
| EP (1) | EP0221308B1 (enExample) |
| JP (1) | JPS6281565A (enExample) |
| CA (1) | CA1288709C (enExample) |
| DE (1) | DE3666707D1 (enExample) |
Families Citing this family (77)
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| DE3850273T2 (de) * | 1987-03-02 | 1994-09-29 | Gen Probe Inc | Polykationische Träger zur Reinigung, Trennung und Hybridisierung von Nukleinsäure. |
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| JPH084519B2 (ja) * | 1987-12-25 | 1996-01-24 | エフ・ホフマン―ラ ロシュ アーゲー | ハイブリッド形成用担体およびその調整方法 |
| CA1325761C (en) * | 1987-12-25 | 1994-01-04 | Takanori Oka | Method of detecting an intended nucleic acid in a sample |
| US4952519A (en) * | 1988-05-02 | 1990-08-28 | E. I. Du Pont De Nemours And Company | Protein immobilization with poly(ethyleneimine) derivatized with a hydroprobic group |
| JP2667447B2 (ja) * | 1988-06-28 | 1997-10-27 | オリンパス光学工業株式会社 | 固体表面への細胞の固定化方法 |
| US5047523A (en) * | 1988-08-02 | 1991-09-10 | Ortho Diagnostic Systems Inc. | Nucleic acid probe for detection of neisseria gonorrhoea |
| JP2739068B2 (ja) * | 1988-09-24 | 1998-04-08 | 春馬 川口 | Ｄｎａ固定ミクロスフェア及びそれを用いるｄｎａ転写制御因子精製法 |
| US5972386A (en) * | 1995-12-19 | 1999-10-26 | Flinders Technologies Pty, Ltd. | Dry solid medium for storage and analysis of genetic material |
| WO1990003959A1 (en) * | 1988-10-05 | 1990-04-19 | The Flinders University Of South Australia | Solid medium and method for dna storage |
| US6447804B1 (en) | 1988-10-05 | 2002-09-10 | Whatman, Plc | Dry solid medium for storage and analysis of genetic material |
| US5496562A (en) * | 1988-10-05 | 1996-03-05 | Flinders Technologies Pty Ltd | Solid medium and method for DNA storage |
| US5807527A (en) * | 1991-05-29 | 1998-09-15 | Flinders Technologies Pty. Ltd. | Solid medium and method for DNA storage |
| US6627226B2 (en) | 1988-10-05 | 2003-09-30 | Whatman, Inc. | Dry solid medium for storage and analysis of genetic material |
| US5985327A (en) * | 1988-10-05 | 1999-11-16 | Flinders Technologies Pty. Ltd. | Solid medium and method for DNA storage |
| US5756126A (en) * | 1991-05-29 | 1998-05-26 | Flinders Technologies Pty. Ltd. | Dry solid medium for storage and analysis of genetic material |
| US5683916A (en) * | 1988-10-31 | 1997-11-04 | Hemasure Inc. | Membrane affinity apparatus and purification methods related thereto |
| US5324829A (en) * | 1988-12-16 | 1994-06-28 | Ortho Diagnostic Systems, Inc. | High specific activity nucleic acid probes having target recognition and signal generating moieties |
| US5030561A (en) * | 1988-12-27 | 1991-07-09 | Becton, Dickinson And Company | Chlamydia assay using amidine modified supports or particles |
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| EP0456765A1 (en) * | 1989-01-31 | 1991-11-21 | E.I. Du Pont De Nemours And Company | Porous substrates with a high concentration of amine groups |
| US5559221A (en) * | 1989-02-15 | 1996-09-24 | Worcester Foundation For Experimental Biology | Method of separating oligonucleotides from a mixture |
| US5352578A (en) * | 1989-02-15 | 1994-10-04 | Worcester Foundation For Experimental Biology | Method of separating oligonucleotides from a mixture |
| DE69032847T2 (de) * | 1989-06-30 | 1999-05-12 | Chiron Corp., Emeryville, Calif. | Hydrophobe Nukleinsäure-Sonde |
| JPH03257369A (ja) * | 1990-02-27 | 1991-11-15 | Yon Rin Mo | 簡単なイムノドットニトロセルロースまたはナイロンペーパー |
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| US5667976A (en) * | 1990-05-11 | 1997-09-16 | Becton Dickinson And Company | Solid supports for nucleic acid hybridization assays |
| WO1992008808A1 (en) * | 1990-11-14 | 1992-05-29 | Siska Diagnostics, Inc. | Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor |
| EP0834575B1 (en) * | 1990-12-06 | 2001-11-28 | Affymetrix, Inc. (a Delaware Corporation) | Identification of nucleic acids in samples |
| FR2679255B1 (fr) * | 1991-07-17 | 1993-10-22 | Bio Merieux | Procede d'immobilisation d'un fragment nucleique par fixation passive sur un support solide, support solide ainsi obtenu et son utilisation. |
| GB9208548D0 (en) * | 1992-04-21 | 1992-06-03 | Kodak Ltd | An immunoassay and test kit |
| US5795714A (en) * | 1992-11-06 | 1998-08-18 | Trustees Of Boston University | Method for replicating an array of nucleic acid probes |
| US6436635B1 (en) * | 1992-11-06 | 2002-08-20 | Boston University | Solid phase sequencing of double-stranded nucleic acids |
| US5605798A (en) | 1993-01-07 | 1997-02-25 | Sequenom, Inc. | DNA diagnostic based on mass spectrometry |
| US6194144B1 (en) | 1993-01-07 | 2001-02-27 | Sequenom, Inc. | DNA sequencing by mass spectrometry |
| US6893816B1 (en) * | 1993-10-28 | 2005-05-17 | Houston Advanced Research Center | Microfabricated, flowthrough porous apparatus for discrete detection of binding reactions |
| US6428955B1 (en) | 1995-03-17 | 2002-08-06 | Sequenom, Inc. | DNA diagnostics based on mass spectrometry |
| US7803529B1 (en) | 1995-04-11 | 2010-09-28 | Sequenom, Inc. | Solid phase sequencing of biopolymers |
| US20060063193A1 (en) * | 1995-04-11 | 2006-03-23 | Dong-Jing Fu | Solid phase sequencing of double-stranded nucleic acids |
| US5830655A (en) | 1995-05-22 | 1998-11-03 | Sri International | Oligonucleotide sizing using cleavable primers |
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| CA2348054C (en) * | 1998-10-23 | 2012-06-19 | Qiagen Gesellschaft Mit Beschrankter Haftung | Processes and means for the isolation and purification of nucleic acids at surfaces |
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| AU2004290375A1 (en) * | 2003-11-06 | 2005-05-26 | Sru Biosystems, Inc. | High-density amine-functionalized surface |
| WO2005102526A1 (en) | 2004-04-09 | 2005-11-03 | Research Think Tank, Inc. | Devices and methods for collection, storage and transportation of biological specimens |
| CN101346185A (zh) * | 2005-12-21 | 2009-01-14 | 皇家飞利浦电子股份有限公司 | 生物分子的传感器和使用所述传感器的分析方法 |
| WO2008055080A2 (en) * | 2006-10-31 | 2008-05-08 | Sru Biosystems, Inc. | Method for blocking non-specific protein binding on a functionalized surface |
| US20090137024A1 (en) * | 2007-11-28 | 2009-05-28 | Canon U.S. Life Sciences, Inc. | Method of Separating Target DNA from Mixed DNA |
| CN119866372A (zh) * | 2022-10-31 | 2025-04-22 | 深圳华大智造科技股份有限公司 | 一种核酸加载试剂及用其检测测序芯片吸附能力的方法 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1550819A (en) * | 1975-06-04 | 1979-08-22 | Nat Res Dev | Polymeric support for biogically active materials |
| FR2422956A1 (fr) * | 1978-04-13 | 1979-11-09 | Pasteur Institut | Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede |
| JPS5510590A (en) * | 1978-05-04 | 1980-01-25 | Wellcome Found | Enzyme immunity quantity analysis |
| US4455370A (en) * | 1982-05-17 | 1984-06-19 | E. I. Du Pont De Nemours And Company | Transferring separated components in gel electrophoresis via nylon membrane |
| US4588682A (en) * | 1982-12-13 | 1986-05-13 | Integrated Genetics, Inc. | Binding nucleic acid to a support |
| US4512896A (en) * | 1983-02-07 | 1985-04-23 | Yale University | Transfer of macromolecules from a chromatographic substrate to an immobilizing matrix |
| IL71715A0 (en) * | 1983-08-05 | 1984-09-30 | Miles Lab | Method,gene probes and test kit for detecting bacteria by nucleic acid hybridization |
| US4652517A (en) * | 1984-06-11 | 1987-03-24 | Diagnostic Research Limited Partnership | Methods for the in vitro detection and identification of unknown pathogens or genetic entities |
| FR2565996B1 (fr) * | 1984-06-15 | 1988-01-22 | Inst Nat Sante Rech Med | Acides nucleiques marques chimiquement, leur utilisation et necessaire pour sa mise en oeuvre |
| US4563417A (en) * | 1984-08-31 | 1986-01-07 | Miles Laboratories, Inc. | Nucleic acid hybridization assay employing antibodies to intercalation complexes |
-
1985
- 1985-09-30 US US06/781,332 patent/US4806546A/en not_active Expired - Fee Related
-
1986
- 1986-06-13 CA CA000511592A patent/CA1288709C/en not_active Expired - Lifetime
- 1986-09-18 EP EP86112900A patent/EP0221308B1/en not_active Expired
- 1986-09-18 DE DE8686112900T patent/DE3666707D1/de not_active Expired
- 1986-09-25 JP JP61225059A patent/JPS6281565A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| DE3666707D1 (en) | 1989-12-07 |
| EP0221308A1 (en) | 1987-05-13 |
| EP0221308B1 (en) | 1989-11-02 |
| US4806546A (en) | 1989-02-21 |
| JPS6281565A (ja) | 1987-04-15 |
| JPH0516838B2 (enExample) | 1993-03-05 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKLA | Lapsed | ||
| MKLA | Lapsed |
Effective date: 19970910 |