JPH0421819B2 - - Google Patents
Info
- Publication number
- JPH0421819B2 JPH0421819B2 JP56194336A JP19433681A JPH0421819B2 JP H0421819 B2 JPH0421819 B2 JP H0421819B2 JP 56194336 A JP56194336 A JP 56194336A JP 19433681 A JP19433681 A JP 19433681A JP H0421819 B2 JPH0421819 B2 JP H0421819B2
- Authority
- JP
- Japan
- Prior art keywords
- antibody
- antibodies
- antigen
- labeled
- antigenic substance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 claims description 41
- 239000000126 substance Substances 0.000 claims description 41
- 230000000890 antigenic effect Effects 0.000 claims description 33
- 239000007787 solid Substances 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 11
- 238000013198 immunometric assay Methods 0.000 claims 1
- 239000007791 liquid phase Substances 0.000 claims 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical group [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 claims 1
- 239000000427 antigen Substances 0.000 description 85
- 108091007433 antigens Proteins 0.000 description 85
- 102000036639 antigens Human genes 0.000 description 85
- 238000003556 assay Methods 0.000 description 62
- 239000000523 sample Substances 0.000 description 45
- 238000012360 testing method Methods 0.000 description 31
- 239000002245 particle Substances 0.000 description 26
- 230000015572 biosynthetic process Effects 0.000 description 20
- 238000006243 chemical reaction Methods 0.000 description 15
- 238000003018 immunoassay Methods 0.000 description 15
- 238000011534 incubation Methods 0.000 description 15
- 239000007790 solid phase Substances 0.000 description 14
- 238000001514 detection method Methods 0.000 description 13
- 239000012530 fluid Substances 0.000 description 13
- 229920000936 Agarose Polymers 0.000 description 12
- 230000002163 immunogen Effects 0.000 description 11
- 102000004190 Enzymes Human genes 0.000 description 10
- 108090000790 Enzymes Proteins 0.000 description 10
- 229940088598 enzyme Drugs 0.000 description 10
- 238000010791 quenching Methods 0.000 description 10
- 230000000171 quenching effect Effects 0.000 description 10
- 230000005764 inhibitory process Effects 0.000 description 9
- 230000002441 reversible effect Effects 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 8
- 210000002966 serum Anatomy 0.000 description 8
- 239000000463 material Substances 0.000 description 7
- 238000005406 washing Methods 0.000 description 7
- 239000000203 mixture Substances 0.000 description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 5
- 230000035945 sensitivity Effects 0.000 description 5
- 239000000758 substrate Substances 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- 102000012406 Carcinoembryonic Antigen Human genes 0.000 description 4
- 108010022366 Carcinoembryonic Antigen Proteins 0.000 description 4
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 210000004408 hybridoma Anatomy 0.000 description 4
- 230000001900 immune effect Effects 0.000 description 4
- 210000000987 immune system Anatomy 0.000 description 4
- 239000004816 latex Substances 0.000 description 4
- 229920000126 latex Polymers 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 102000005548 Hexokinase Human genes 0.000 description 3
- 108700040460 Hexokinases Proteins 0.000 description 3
- 102000011923 Thyrotropin Human genes 0.000 description 3
- 108010061174 Thyrotropin Proteins 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000004220 aggregation Methods 0.000 description 3
- 230000002776 aggregation Effects 0.000 description 3
- 230000003281 allosteric effect Effects 0.000 description 3
- 238000006555 catalytic reaction Methods 0.000 description 3
- 230000009260 cross reactivity Effects 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 208000006454 hepatitis Diseases 0.000 description 3
- 239000013642 negative control Substances 0.000 description 3
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 3
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 3
- 238000003127 radioimmunoassay Methods 0.000 description 3
- 239000004576 sand Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 108010032595 Antibody Binding Sites Proteins 0.000 description 2
- 108010015776 Glucose oxidase Proteins 0.000 description 2
- 239000004366 Glucose oxidase Substances 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 102000003992 Peroxidases Human genes 0.000 description 2
- 241000276498 Pollachius virens Species 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 239000013068 control sample Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- FVTCRASFADXXNN-SCRDCRAPSA-N flavin mononucleotide Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O FVTCRASFADXXNN-SCRDCRAPSA-N 0.000 description 2
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 2
- 229940116332 glucose oxidase Drugs 0.000 description 2
- 235000019420 glucose oxidase Nutrition 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 231100000283 hepatitis Toxicity 0.000 description 2
- 238000010324 immunological assay Methods 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 108040007629 peroxidase activity proteins Proteins 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- -1 polyethylene Polymers 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 230000002285 radioactive effect Effects 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000002798 spectrophotometry method Methods 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 description 1
- KGLZWCCDTHHPLO-UHFFFAOYSA-N 2,3-dihydropyridine-3-carboxamide Chemical compound NC(=O)C1CN=CC=C1 KGLZWCCDTHHPLO-UHFFFAOYSA-N 0.000 description 1
- ACNUVXZPCIABEX-UHFFFAOYSA-N 3',6'-diaminospiro[2-benzofuran-3,9'-xanthene]-1-one Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(N)C=C1OC1=CC(N)=CC=C21 ACNUVXZPCIABEX-UHFFFAOYSA-N 0.000 description 1
- 102100031126 6-phosphogluconolactonase Human genes 0.000 description 1
- 108010029731 6-phosphogluconolactonase Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- NBSCHQHZLSJFNQ-GASJEMHNSA-N D-Glucose 6-phosphate Chemical compound OC1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H](O)[C@H]1O NBSCHQHZLSJFNQ-GASJEMHNSA-N 0.000 description 1
- PHOQVHQSTUBQQK-SQOUGZDYSA-N D-glucono-1,5-lactone Chemical compound OC[C@H]1OC(=O)[C@H](O)[C@@H](O)[C@@H]1O PHOQVHQSTUBQQK-SQOUGZDYSA-N 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- VFRROHXSMXFLSN-UHFFFAOYSA-N Glc6P Natural products OP(=O)(O)OCC(O)C(O)C(O)C(O)C=O VFRROHXSMXFLSN-UHFFFAOYSA-N 0.000 description 1
- 108010018962 Glucosephosphate Dehydrogenase Proteins 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 241001490312 Lithops pseudotruncatella Species 0.000 description 1
- 108060001084 Luciferase Proteins 0.000 description 1
- 239000005089 Luciferase Substances 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 102000001105 Phosphofructokinases Human genes 0.000 description 1
- 108010069341 Phosphofructokinases Proteins 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 238000003450 affinity purification method Methods 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 239000013566 allergen Substances 0.000 description 1
- 230000008850 allosteric inhibition Effects 0.000 description 1
- 102000013529 alpha-Fetoproteins Human genes 0.000 description 1
- 108010026331 alpha-Fetoproteins Proteins 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 210000002798 bone marrow cell Anatomy 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000012876 carrier material Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000005515 coenzyme Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 238000002967 competitive immunoassay Methods 0.000 description 1
- 230000009918 complex formation Effects 0.000 description 1
- 238000010668 complexation reaction Methods 0.000 description 1
- 230000001268 conjugating effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 230000005281 excited state Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000002795 fluorescence method Methods 0.000 description 1
- 108010074605 gamma-Globulins Proteins 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 235000012209 glucono delta-lactone Nutrition 0.000 description 1
- 229960003681 gluconolactone Drugs 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 210000004754 hybrid cell Anatomy 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 238000002796 luminescence method Methods 0.000 description 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000036963 noncompetitive effect Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000011236 particulate material Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 150000004986 phenylenediamines Chemical class 0.000 description 1
- 239000002953 phosphate buffered saline Substances 0.000 description 1
- 229930029653 phosphoenolpyruvate Natural products 0.000 description 1
- DTBNBXWJWCWCIK-UHFFFAOYSA-N phosphoenolpyruvic acid Chemical compound OC(=O)C(=C)OP(O)(O)=O DTBNBXWJWCWCIK-UHFFFAOYSA-N 0.000 description 1
- 208000024335 physical disease Diseases 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000000941 radioactive substance Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- 238000004879 turbidimetry Methods 0.000 description 1
- 231100000611 venom Toxicity 0.000 description 1
- 239000002435 venom Substances 0.000 description 1
- 210000001048 venom Anatomy 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Landscapes
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US06/323,498 US4486530A (en) | 1980-08-04 | 1981-06-24 | Immunometric assays using monoclonal antibodies |
| US323498 | 1981-06-24 |
Related Child Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP12129789A Division JPH0235365A (ja) | 1981-06-24 | 1989-05-15 | 抗原性物質の存在もしくはその濃度を決定する方法 |
| JP30785091A Division JPH0572208A (ja) | 1981-06-24 | 1991-11-22 | 単クローン性抗体を使用する免疫学的、抑制試験 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS585659A JPS585659A (ja) | 1983-01-13 |
| JPH0421819B2 true JPH0421819B2 (pt) | 1992-04-14 |
Family
ID=23259453
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP19433681A Granted JPS585659A (ja) | 1981-06-24 | 1981-12-02 | 単クロ−ン性抗体を使用する免疫学的、抑制試験 |
| JP12129789A Pending JPH0235365A (ja) | 1981-06-24 | 1989-05-15 | 抗原性物質の存在もしくはその濃度を決定する方法 |
| JP30785091A Pending JPH0572208A (ja) | 1981-06-24 | 1991-11-22 | 単クローン性抗体を使用する免疫学的、抑制試験 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP12129789A Pending JPH0235365A (ja) | 1981-06-24 | 1989-05-15 | 抗原性物質の存在もしくはその濃度を決定する方法 |
| JP30785091A Pending JPH0572208A (ja) | 1981-06-24 | 1991-11-22 | 単クローン性抗体を使用する免疫学的、抑制試験 |
Country Status (1)
| Country | Link |
|---|---|
| JP (3) | JPS585659A (pt) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS585659A (ja) * | 1981-06-24 | 1983-01-13 | ハイブリテツク インコ−ポレ−テツド | 単クロ−ン性抗体を使用する免疫学的、抑制試験 |
| JPS59219738A (ja) * | 1983-05-30 | 1984-12-11 | Ricoh Co Ltd | 複写機光学系変倍装置 |
| DE3644207A1 (de) * | 1986-12-23 | 1988-07-07 | Satzinger Gebhard Gmbh Co | Vorrichtung zum kontinuierlichen zufuehren von fluessigen oder viskosen medien, insbesondere von schmierstoffen |
| AU1232188A (en) * | 1987-05-29 | 1988-12-01 | Mallinckrodt, Inc. | Novel pair of monoclonal antibodies to insulin-like growth factor i permits immunometric assay for igf-i |
| CA2021658C (en) * | 1989-08-25 | 2001-10-09 | Myron J. Block | Multiplex immunoassay system |
| JPH03118472A (ja) * | 1989-09-29 | 1991-05-21 | Sekisui Chem Co Ltd | インスリン定量方法及び定量試薬 |
| JP2516314Y2 (ja) * | 1990-10-15 | 1996-11-06 | 日本シイエムケイ株式会社 | 電磁波シールドプリント配線板 |
| JPH05152781A (ja) * | 1991-06-24 | 1993-06-18 | Cmk Corp | プリント配線基板の製造法 |
| DE4309393A1 (de) * | 1993-03-23 | 1994-09-29 | Boehringer Mannheim Gmbh | Verringerung des Hook-Effekts in Immuntests mit teilchenförmigem Trägermaterial |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5144628A (pt) * | 1974-08-12 | 1976-04-16 | Syva Co | |
| JPS54136896A (en) * | 1978-04-05 | 1979-10-24 | Syva Co | Method of analyzing chemically produced fluorescence immunity |
| US4174384A (en) * | 1975-06-30 | 1979-11-13 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
| JPS54151894A (en) * | 1978-04-05 | 1979-11-29 | Syva Co | Method of analyzing chemicallyyproduced immunity |
| JPS5786051A (en) * | 1980-07-28 | 1982-05-28 | Akzo Nv | Determination of antigen employing two or more monochronal antibodies |
| JPS57118159A (en) * | 1980-08-04 | 1982-07-22 | Hybritech Inc | Immunological inhibition test using signle coulomb- oriented antibody |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4120945A (en) * | 1976-07-06 | 1978-10-17 | Becton, Dickinson & Company | Substrate coated with receptor and labeled ligand for assays |
| JPS5344622A (en) * | 1976-09-30 | 1978-04-21 | Mochida Pharm Co Ltd | Immunologically measuring method |
| WO1982000058A1 (en) * | 1980-06-20 | 1982-01-07 | Porter P | Processes and apparatus for carrying out specific binding assays |
| JPS585659A (ja) * | 1981-06-24 | 1983-01-13 | ハイブリテツク インコ−ポレ−テツド | 単クロ−ン性抗体を使用する免疫学的、抑制試験 |
-
1981
- 1981-12-02 JP JP19433681A patent/JPS585659A/ja active Granted
-
1989
- 1989-05-15 JP JP12129789A patent/JPH0235365A/ja active Pending
-
1991
- 1991-11-22 JP JP30785091A patent/JPH0572208A/ja active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5144628A (pt) * | 1974-08-12 | 1976-04-16 | Syva Co | |
| US4174384A (en) * | 1975-06-30 | 1979-11-13 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
| JPS54136896A (en) * | 1978-04-05 | 1979-10-24 | Syva Co | Method of analyzing chemically produced fluorescence immunity |
| JPS54151894A (en) * | 1978-04-05 | 1979-11-29 | Syva Co | Method of analyzing chemicallyyproduced immunity |
| JPS5786051A (en) * | 1980-07-28 | 1982-05-28 | Akzo Nv | Determination of antigen employing two or more monochronal antibodies |
| JPS57118159A (en) * | 1980-08-04 | 1982-07-22 | Hybritech Inc | Immunological inhibition test using signle coulomb- oriented antibody |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0572208A (ja) | 1993-03-23 |
| JPS585659A (ja) | 1983-01-13 |
| JPH0235365A (ja) | 1990-02-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JPH0421818B2 (pt) | ||
| US4486530A (en) | Immunometric assays using monoclonal antibodies | |
| Yolken | Enzyme-linked immunosorbent assay (ELISA): a practical tool for rapid diagnosis of viruses and other infectious agents. | |
| FI92110B (fi) | Useiden analyyttien vallitsevien konsentraatioiden määrittäminen | |
| CA1254828A (en) | Sandwich immunoassay | |
| CA2200683A1 (en) | Method for detecting antibodies | |
| IE890683L (en) | Method for determination of a polyvalent substance using an immunoaggregate | |
| FI89984B (fi) | Enstegsimmuntest foer bestaemning av antigenspecifika antikroppar hoerande till immunoglobulingruppen a, m, d eller e och medel daerfoer | |
| EP0149602A1 (en) | Immunometric assay using polyclonal and monoclonal antibodies and a kit for use therein | |
| US4138213A (en) | Agglutination immunoassay of immune complex with RF or Clq | |
| EP0124366B1 (en) | Method of measuring biological ligands | |
| JPH0421819B2 (pt) | ||
| JPH0792460B2 (ja) | 抽出組成物として界面活性剤混合物を使用する歯周病に随伴する微生物検出用キットおよびその検出方法 | |
| JP2596574B2 (ja) | クレアチンキナーゼｍｂ定量方法 | |
| JPH0476628B2 (pt) | ||
| EP0248038A1 (en) | Idiotypic-antigenic conjunction binding assay | |
| JP4556605B2 (ja) | 標的物質の測定方法および測定試薬 | |
| CA1289874C (en) | Anti-enzyme antibody immunoassay | |
| JP3569074B2 (ja) | イムノアッセイ法 | |
| JPS6252466A (ja) | 不均一イムノアッセイを行う方法 | |
| JPS5890169A (ja) | 単クロ−ン性抗体を使用する免疫学的、抑制試験 | |
| JPH1144688A (ja) | 蛍光偏光免疫測定法 | |
| CA1195926A (en) | Immunometric and inhibition assays using monoclonal antibodies | |
| Salomons et al. | Immunoassay: A survey of patents, patent applications and other literature 1980-1991 | |
| JPS59203958A (ja) | α↓１−プロティナ−ゼインヒビタ−・カリクレイン結合物測定用試薬 |