JPH0357423B2 - - Google Patents
Info
- Publication number
- JPH0357423B2 JPH0357423B2 JP59201273A JP20127384A JPH0357423B2 JP H0357423 B2 JPH0357423 B2 JP H0357423B2 JP 59201273 A JP59201273 A JP 59201273A JP 20127384 A JP20127384 A JP 20127384A JP H0357423 B2 JPH0357423 B2 JP H0357423B2
- Authority
- JP
- Japan
- Prior art keywords
- blood cells
- reagent
- blood cell
- red blood
- alkyl group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 210000000601 blood cell Anatomy 0.000 claims description 33
- 239000003153 chemical reaction reagent Substances 0.000 claims description 23
- 239000003795 chemical substances by application Substances 0.000 claims description 12
- 239000007850 fluorescent dye Substances 0.000 claims description 9
- 125000000217 alkyl group Chemical group 0.000 claims description 8
- 238000005259 measurement Methods 0.000 claims description 8
- 239000002736 nonionic surfactant Substances 0.000 claims description 7
- 239000003093 cationic surfactant Substances 0.000 claims description 6
- 125000004432 carbon atom Chemical group C* 0.000 claims description 4
- 239000007864 aqueous solution Substances 0.000 claims description 2
- 125000002091 cationic group Chemical group 0.000 claims description 2
- 229910052736 halogen Inorganic materials 0.000 claims description 2
- 150000002367 halogens Chemical class 0.000 claims description 2
- 210000003743 erythrocyte Anatomy 0.000 description 27
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 18
- 210000001995 reticulocyte Anatomy 0.000 description 14
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
- 210000001772 blood platelet Anatomy 0.000 description 12
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 10
- 238000010186 staining Methods 0.000 description 9
- 210000004369 blood Anatomy 0.000 description 8
- 239000008280 blood Substances 0.000 description 8
- 238000000684 flow cytometry Methods 0.000 description 8
- 238000010586 diagram Methods 0.000 description 7
- 230000003204 osmotic effect Effects 0.000 description 6
- 239000011780 sodium chloride Substances 0.000 description 6
- 239000012192 staining solution Substances 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- DPKHZNPWBDQZCN-UHFFFAOYSA-N acridine orange free base Chemical compound C1=CC(N(C)C)=CC2=NC3=CC(N(C)C)=CC=C3C=C21 DPKHZNPWBDQZCN-UHFFFAOYSA-N 0.000 description 5
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 5
- DZBUGLKDJFMEHC-UHFFFAOYSA-N benzoquinolinylidene Natural products C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 description 5
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000000975 dye Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000000834 fixative Substances 0.000 description 3
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 3
- 230000000638 stimulation Effects 0.000 description 3
- ADAOOVVYDLASGJ-UHFFFAOYSA-N 2,7,10-trimethylacridin-10-ium-3,6-diamine;chloride Chemical compound [Cl-].CC1=C(N)C=C2[N+](C)=C(C=C(C(C)=C3)N)C3=CC2=C1 ADAOOVVYDLASGJ-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- CXRFDZFCGOPDTD-UHFFFAOYSA-M Cetrimide Chemical compound [Br-].CCCCCCCCCCCCCC[N+](C)(C)C CXRFDZFCGOPDTD-UHFFFAOYSA-M 0.000 description 2
- -1 alkali metal salt Chemical class 0.000 description 2
- 239000003945 anionic surfactant Substances 0.000 description 2
- 239000003146 anticoagulant agent Substances 0.000 description 2
- 229940127219 anticoagulant drug Drugs 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- INCIMLINXXICKS-UHFFFAOYSA-M pyronin Y Chemical compound [Cl-].C1=CC(=[N+](C)C)C=C2OC3=CC(N(C)C)=CC=C3C=C21 INCIMLINXXICKS-UHFFFAOYSA-M 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- RNMDNPCBIKJCQP-UHFFFAOYSA-N 5-nonyl-7-oxabicyclo[4.1.0]hepta-1,3,5-trien-2-ol Chemical compound C(CCCCCCCC)C1=C2C(=C(C=C1)O)O2 RNMDNPCBIKJCQP-UHFFFAOYSA-N 0.000 description 1
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- 241000047703 Nonion Species 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 229910052783 alkali metal Inorganic materials 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 239000000981 basic dye Substances 0.000 description 1
- 238000009534 blood test Methods 0.000 description 1
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 1
- 229910052794 bromium Inorganic materials 0.000 description 1
- MPBRYMWMMKKRGC-UHFFFAOYSA-M carbocyanin DBTC Chemical compound [Br-].C1=CC=CC2=C([N+](=C(C=C(C)C=C3N(C4=C5C=CC=CC5=CC=C4S3)CC)S3)CC)C3=CC=C21 MPBRYMWMMKKRGC-UHFFFAOYSA-M 0.000 description 1
- WOWHHFRSBJGXCM-UHFFFAOYSA-M cetyltrimethylammonium chloride Chemical compound [Cl-].CCCCCCCCCCCCCCCC[N+](C)(C)C WOWHHFRSBJGXCM-UHFFFAOYSA-M 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 230000002949 hemolytic effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59201273A JPS6179163A (ja) | 1984-09-26 | 1984-09-26 | 血球測定用試薬 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59201273A JPS6179163A (ja) | 1984-09-26 | 1984-09-26 | 血球測定用試薬 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6179163A JPS6179163A (ja) | 1986-04-22 |
| JPH0357423B2 true JPH0357423B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1991-09-02 |
Family
ID=16438222
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP59201273A Granted JPS6179163A (ja) | 1984-09-26 | 1984-09-26 | 血球測定用試薬 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6179163A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2514087B2 (ja) * | 1989-01-06 | 1996-07-10 | 富士写真フイルム株式会社 | 総コレステロ―ル分析要素 |
| US5478722A (en) * | 1991-02-17 | 1995-12-26 | The Curators Of The University Of Missouri | Preserved cell preparations for flow cytometry and immunology |
| JP2913219B2 (ja) * | 1991-03-06 | 1999-06-28 | 株式会社シマ研究所 | 尿沈渣検査成績管理方法 |
| US5284771A (en) * | 1991-12-05 | 1994-02-08 | Miles Inc. | Reagent compositions and their use in sphering cells |
| US5360739A (en) * | 1991-12-05 | 1994-11-01 | Miles Inc. | Methods for the identification and characterization of reticulocytes in whole blood |
| US5350695A (en) * | 1991-12-05 | 1994-09-27 | Miles Inc. | Methods for the identification and characterization of reticulocytes in whole blood |
| JPH09105744A (ja) * | 1994-10-08 | 1997-04-22 | Koji Aoki | 細胞固定剤入り超生体染色液 |
| JP3425830B2 (ja) * | 1995-10-06 | 2003-07-14 | シスメックス株式会社 | 新規化合物とその用途 |
| TW379284B (en) * | 1996-04-12 | 2000-01-11 | Toa Medical Electronics | Agent for detecting reticulocyte |
| FR2759166B1 (fr) * | 1997-01-31 | 1999-04-23 | Abx Sa | Reactif de coloration pour la determination de cellules sanguines |
| JP3587651B2 (ja) * | 1997-05-09 | 2004-11-10 | シスメックス株式会社 | 粒子測定装置 |
| US6060322A (en) * | 1998-10-20 | 2000-05-09 | Coulter International Corp. | Method for identification of reticulated cells |
| CA2428740A1 (en) * | 2002-05-20 | 2003-11-20 | Bayer Corporation | Automated method and reagent therefor for assaying body fluid samples such as cerebrospinal fluid (csf) |
| JP4794414B2 (ja) * | 2006-10-30 | 2011-10-19 | シスメックス株式会社 | 血小板測定用試薬、血小板測定用試薬キット並びに血小板測定方法 |
| JP4806330B2 (ja) * | 2006-10-30 | 2011-11-02 | シスメックス株式会社 | 網状赤血球及び血小板測定用試薬、網状赤血球及び血小板測定用試薬キット並びに網状赤血球及び血小板測定方法 |
| EP3104177B1 (en) * | 2011-12-21 | 2020-11-25 | Beckman Coulter, Inc. | Method for labeling intracellular and extracellular targets of leukocytes |
-
1984
- 1984-09-26 JP JP59201273A patent/JPS6179163A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6179163A (ja) | 1986-04-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |