JPH0336508B2 - - Google Patents
Info
- Publication number
- JPH0336508B2 JPH0336508B2 JP6283085A JP6283085A JPH0336508B2 JP H0336508 B2 JPH0336508 B2 JP H0336508B2 JP 6283085 A JP6283085 A JP 6283085A JP 6283085 A JP6283085 A JP 6283085A JP H0336508 B2 JPH0336508 B2 JP H0336508B2
- Authority
- JP
- Japan
- Prior art keywords
- electric field
- cells
- conductive path
- fine particles
- polarizable
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 230000005684 electric field Effects 0.000 claims description 39
- 239000010419 fine particle Substances 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 15
- 238000006073 displacement reaction Methods 0.000 claims description 14
- 230000007910 cell fusion Effects 0.000 claims description 13
- 239000013598 vector Substances 0.000 claims description 9
- 239000003094 microcapsule Substances 0.000 claims description 8
- 230000002776 aggregation Effects 0.000 claims description 7
- 238000004220 aggregation Methods 0.000 claims description 7
- 239000008151 electrolyte solution Substances 0.000 claims description 7
- 239000000126 substance Substances 0.000 claims description 7
- 239000011859 microparticle Substances 0.000 claims description 4
- 239000000615 nonconductor Substances 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 230000007423 decrease Effects 0.000 claims 1
- 230000001939 inductive effect Effects 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 77
- 239000011521 glass Substances 0.000 description 7
- 230000004927 fusion Effects 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 239000000725 suspension Substances 0.000 description 6
- 230000004907 flux Effects 0.000 description 5
- 102000053602 DNA Human genes 0.000 description 4
- 108020004414 DNA Proteins 0.000 description 4
- 238000004720 dielectrophoresis Methods 0.000 description 4
- 210000000170 cell membrane Anatomy 0.000 description 3
- WABPQHHGFIMREM-UHFFFAOYSA-N lead(0) Chemical compound [Pb] WABPQHHGFIMREM-UHFFFAOYSA-N 0.000 description 3
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 3
- 230000000638 stimulation Effects 0.000 description 3
- 239000004925 Acrylic resin Substances 0.000 description 2
- 229920000178 Acrylic resin Polymers 0.000 description 2
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- 229930195725 Mannitol Natural products 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 239000010410 layer Substances 0.000 description 2
- 239000000594 mannitol Substances 0.000 description 2
- 235000010355 mannitol Nutrition 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 210000001938 protoplast Anatomy 0.000 description 2
- 229920002799 BoPET Polymers 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000005041 Mylar™ Substances 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 238000003163 cell fusion method Methods 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- GTKRFUAGOKINCA-UHFFFAOYSA-M chlorosilver;silver Chemical compound [Ag].[Ag]Cl GTKRFUAGOKINCA-UHFFFAOYSA-M 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012777 electrically insulating material Substances 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 239000007772 electrode material Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- -1 for example Substances 0.000 description 1
- 238000007499 fusion processing Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000011810 insulating material Substances 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000010445 mica Substances 0.000 description 1
- 229910052618 mica group Inorganic materials 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 239000006174 pH buffer Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6283085A JPS61219386A (ja) | 1985-03-26 | 1985-03-26 | 分極性微粒体の集合方法および装置 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6283085A JPS61219386A (ja) | 1985-03-26 | 1985-03-26 | 分極性微粒体の集合方法および装置 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61219386A JPS61219386A (ja) | 1986-09-29 |
| JPH0336508B2 true JPH0336508B2 (enrdf_load_stackoverflow) | 1991-05-31 |
Family
ID=13211626
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP6283085A Granted JPS61219386A (ja) | 1985-03-26 | 1985-03-26 | 分極性微粒体の集合方法および装置 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61219386A (enrdf_load_stackoverflow) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH074218B2 (ja) * | 1987-01-24 | 1995-01-25 | 株式会社アドバンス | 細胞融合装置 |
| JP2619387B2 (ja) * | 1987-04-30 | 1997-06-11 | 株式会社日立製作所 | 細胞融合方法 |
| JPS63178495U (enrdf_load_stackoverflow) * | 1987-05-11 | 1988-11-18 | ||
| JPH0782009B2 (ja) * | 1988-03-24 | 1995-09-06 | 工業技術院長 | 細胞融合方法及び装置 |
| JP4504501B2 (ja) * | 2000-03-15 | 2010-07-14 | 株式会社東海理化電機製作所 | 粒子移動／固定装置 |
| JP4677832B2 (ja) * | 2005-06-13 | 2011-04-27 | 東ソー株式会社 | 細胞融合用微小流路基板及びそれを用いた細胞融合用微小流路構造体並びに細胞融合方法 |
| JP4918811B2 (ja) * | 2005-06-13 | 2012-04-18 | 東ソー株式会社 | 細胞融合用チャンバーおよび細胞融合装置、並びにそれらを用いた細胞融合方法 |
| JP2007296510A (ja) * | 2006-04-03 | 2007-11-15 | Tosoh Corp | 微粒子操作装置及び微粒子操作方法 |
| JP4973305B2 (ja) * | 2006-05-11 | 2012-07-11 | 東ソー株式会社 | 細胞融合装置及びそれを用いた細胞融合方法 |
| JP2008260008A (ja) * | 2007-03-19 | 2008-10-30 | Tosoh Corp | 微粒子操作装置及びそれを用いた微粒子操作方法 |
| JP7450871B2 (ja) * | 2019-08-28 | 2024-03-18 | 株式会社日立製作所 | 細胞製造装置 |
-
1985
- 1985-03-26 JP JP6283085A patent/JPS61219386A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS61219386A (ja) | 1986-09-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4923814A (en) | High speed, high power apparatus for vesicle prealignment, poration, loading and fusion in uniform electric fields and method therefor | |
| JPH0336508B2 (enrdf_load_stackoverflow) | ||
| Zimmermann | Electrofusion of cells: principles and industrial potential | |
| JPH05506605A (ja) | 固体、半固体または液体物質の操作 | |
| KR101598847B1 (ko) | 액적의 직접 충전 및 전기영동에 기반한 전기천공 기기, 장치 및 방법 | |
| EP1093515A1 (en) | Electrofusion chamber | |
| CN105143436B (zh) | 电穿孔方法与设备 | |
| JPH01141582A (ja) | 遺伝子導入装置 | |
| Matsue et al. | Microring-ring electrode for manipulation of a single cell | |
| US4784954A (en) | Procedure and device for the fusion of cells | |
| JPH01141583A (ja) | 細胞処理チャンバ | |
| CN219621187U (zh) | 一种细胞电穿孔装置 | |
| JPH0687782B2 (ja) | 細胞電気穿孔法およびその装置 | |
| US20130089929A1 (en) | Microdevice for fusing cells | |
| CN211079184U (zh) | 一种结合了超声和电流脉冲的转染装置 | |
| JP4918811B2 (ja) | 細胞融合用チャンバーおよび細胞融合装置、並びにそれらを用いた細胞融合方法 | |
| JP2590459B2 (ja) | 細胞捕獲装置 | |
| JPH0740914B2 (ja) | 電気細胞融合用電極 | |
| US20030141294A1 (en) | Electrofusion chamber | |
| JPS6349071A (ja) | 多極電極型細胞融合チヤンバ | |
| JPS6349068A (ja) | 電気式遺伝子導入装置 | |
| CN115820414A (zh) | 一种细胞电穿孔装置 | |
| Yakovenko | Electroporators based on digital formation of arbitrarily-shaped electroporation pulses | |
| JP2565463B2 (ja) | 電気的細胞操作装置のための操作チャンバー | |
| JPH0324078Y2 (enrdf_load_stackoverflow) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |