JPH032520B2 - - Google Patents
Info
- Publication number
- JPH032520B2 JPH032520B2 JP57136332A JP13633282A JPH032520B2 JP H032520 B2 JPH032520 B2 JP H032520B2 JP 57136332 A JP57136332 A JP 57136332A JP 13633282 A JP13633282 A JP 13633282A JP H032520 B2 JPH032520 B2 JP H032520B2
- Authority
- JP
- Japan
- Prior art keywords
- lower alkyl
- alkyl ester
- culture
- phenylalanine
- ape
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 claims description 28
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 claims description 28
- 125000005907 alkyl ester group Chemical group 0.000 claims description 25
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 24
- 244000005700 microbiome Species 0.000 claims description 18
- 230000001580 bacterial effect Effects 0.000 claims description 17
- 229960005190 phenylalanine Drugs 0.000 claims description 17
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 claims description 15
- 239000001530 fumaric acid Substances 0.000 claims description 14
- 238000004519 manufacturing process Methods 0.000 claims description 14
- 229910021529 ammonia Inorganic materials 0.000 claims description 12
- 238000006243 chemical reaction Methods 0.000 claims description 12
- YZQCXOFQZKCETR-UWVGGRQHSA-N Asp-Phe Chemical compound OC(=O)C[C@H](N)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 YZQCXOFQZKCETR-UWVGGRQHSA-N 0.000 claims description 7
- 241000589516 Pseudomonas Species 0.000 claims description 7
- 125000000217 alkyl group Chemical group 0.000 claims description 3
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 description 20
- 238000000034 method Methods 0.000 description 13
- 239000000047 product Substances 0.000 description 12
- 239000000243 solution Substances 0.000 description 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 6
- 239000007864 aqueous solution Substances 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 239000002994 raw material Substances 0.000 description 5
- 241000894007 species Species 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 4
- -1 N-protected L-aspartic acid anhydride Chemical class 0.000 description 4
- 241000589776 Pseudomonas putida Species 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 229960005261 aspartic acid Drugs 0.000 description 4
- NLVWBYNKMPGKRG-TYYBGVCCSA-N azanium;(e)-4-hydroxy-4-oxobut-2-enoate Chemical compound [NH4+].OC(=O)\C=C\C([O-])=O NLVWBYNKMPGKRG-TYYBGVCCSA-N 0.000 description 4
- VSDUZFOSJDMAFZ-VIFPVBQESA-N methyl L-phenylalaninate Chemical compound COC(=O)[C@@H](N)CC1=CC=CC=C1 VSDUZFOSJDMAFZ-VIFPVBQESA-N 0.000 description 4
- 239000000049 pigment Substances 0.000 description 4
- 125000006239 protecting group Chemical group 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- CKLJMWTZIZZHCS-UHFFFAOYSA-N D-OH-Asp Natural products OC(=O)C(N)CC(O)=O CKLJMWTZIZZHCS-UHFFFAOYSA-N 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- CKLJMWTZIZZHCS-UWTATZPHSA-N L-Aspartic acid Natural products OC(=O)[C@H](N)CC(O)=O CKLJMWTZIZZHCS-UWTATZPHSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 125000003277 amino group Chemical group 0.000 description 3
- IAOZJIPTCAWIRG-QWRGUYRKSA-N aspartame Chemical compound OC(=O)C[C@H](N)C(=O)N[C@H](C(=O)OC)CC1=CC=CC=C1 IAOZJIPTCAWIRG-QWRGUYRKSA-N 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 235000013372 meat Nutrition 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-SVZMEOIVSA-N (+)-Galactose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-SVZMEOIVSA-N 0.000 description 2
- WHBMMWSBFZVSSR-UHFFFAOYSA-N 3-hydroxybutyric acid Chemical compound CC(O)CC(O)=O WHBMMWSBFZVSSR-UHFFFAOYSA-N 0.000 description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- 239000001729 Ammonium fumarate Substances 0.000 description 2
- 108010082340 Arginine deiminase Proteins 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 2
- GLZPCOQZEFWAFX-UHFFFAOYSA-N Geraniol Chemical compound CC(C)=CCCC(C)=CCO GLZPCOQZEFWAFX-UHFFFAOYSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 2
- GKZIWHRNKRBEOH-HOTGVXAUSA-N Phe-Phe Chemical compound C([C@H]([NH3+])C(=O)N[C@@H](CC=1C=CC=CC=1)C([O-])=O)C1=CC=CC=C1 GKZIWHRNKRBEOH-HOTGVXAUSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 2
- 235000019297 ammonium fumarate Nutrition 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- CKKXWJDFFQPBQL-SEPHDYHBSA-N azane;(e)-but-2-enedioic acid Chemical compound N.N.OC(=O)\C=C\C(O)=O CKKXWJDFFQPBQL-SEPHDYHBSA-N 0.000 description 2
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 238000004440 column chromatography Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 239000012531 culture fluid Substances 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 2
- 239000008273 gelatin Substances 0.000 description 2
- 229920000159 gelatin Polymers 0.000 description 2
- 235000019322 gelatine Nutrition 0.000 description 2
- 235000011852 gelatine desserts Nutrition 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 150000004702 methyl esters Chemical class 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- UQDJGEHQDNVPGU-UHFFFAOYSA-N serine phosphoethanolamine Chemical compound [NH3+]CCOP([O-])(=O)OCC([NH3+])C([O-])=O UQDJGEHQDNVPGU-UHFFFAOYSA-N 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 229960004793 sucrose Drugs 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000004809 thin layer chromatography Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 1
- QDGAVODICPCDMU-UHFFFAOYSA-N 2-amino-3-[3-[bis(2-chloroethyl)amino]phenyl]propanoic acid Chemical compound OC(=O)C(N)CC1=CC=CC(N(CCCl)CCCl)=C1 QDGAVODICPCDMU-UHFFFAOYSA-N 0.000 description 1
- 239000004254 Ammonium phosphate Substances 0.000 description 1
- FRYULLIZUDQONW-IMJSIDKUSA-N Asp-Asp Chemical compound OC(=O)C[C@H](N)C(=O)N[C@@H](CC(O)=O)C(O)=O FRYULLIZUDQONW-IMJSIDKUSA-N 0.000 description 1
- 208000035404 Autolysis Diseases 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 206010057248 Cell death Diseases 0.000 description 1
- RFSUNEUAIZKAJO-VRPWFDPXSA-N D-Fructose Natural products OC[C@H]1OC(O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-VRPWFDPXSA-N 0.000 description 1
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 1
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 239000005792 Geraniol Substances 0.000 description 1
- GLZPCOQZEFWAFX-YFHOEESVSA-N Geraniol Natural products CC(C)=CCC\C(C)=C/CO GLZPCOQZEFWAFX-YFHOEESVSA-N 0.000 description 1
- 238000003794 Gram staining Methods 0.000 description 1
- SRBFZHDQGSBBOR-HWQSCIPKSA-N L-arabinopyranose Chemical compound O[C@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-HWQSCIPKSA-N 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 description 1
- 229930064664 L-arginine Natural products 0.000 description 1
- 235000014852 L-arginine Nutrition 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 229910021380 Manganese Chloride Inorganic materials 0.000 description 1
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 1
- 241001645955 Pseudomonas chlororaphis subsp. aureofaciens Species 0.000 description 1
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 1
- 108010046334 Urease Proteins 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 229960003767 alanine Drugs 0.000 description 1
- GZCGUPFRVQAUEE-KVTDHHQDSA-N aldehydo-D-mannose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)C=O GZCGUPFRVQAUEE-KVTDHHQDSA-N 0.000 description 1
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
- 229940024606 amino acid Drugs 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 229910000148 ammonium phosphate Inorganic materials 0.000 description 1
- 235000019289 ammonium phosphates Nutrition 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 150000008064 anhydrides Chemical class 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 125000001584 benzyloxycarbonyl group Chemical group C(=O)(OCC1=CC=CC=C1)* 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 159000000007 calcium salts Chemical class 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 150000001879 copper Chemical class 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000000921 elemental analysis Methods 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 210000003495 flagella Anatomy 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 229940113087 geraniol Drugs 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 229910021389 graphene Inorganic materials 0.000 description 1
- 150000004688 heptahydrates Chemical class 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 159000000003 magnesium salts Chemical class 0.000 description 1
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 1
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 description 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
- 239000011976 maleic acid Substances 0.000 description 1
- 239000001630 malic acid Substances 0.000 description 1
- 235000011090 malic acid Nutrition 0.000 description 1
- 150000002696 manganese Chemical class 0.000 description 1
- 239000011565 manganese chloride Substances 0.000 description 1
- 235000002867 manganese chloride Nutrition 0.000 description 1
- 229940099607 manganese chloride Drugs 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- VMVNZNXAVJHNDJ-UHFFFAOYSA-N methyl 2,2,2-trifluoroacetate Chemical compound COC(=O)C(F)(F)F VMVNZNXAVJHNDJ-UHFFFAOYSA-N 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000011707 mineral Chemical class 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 229910017464 nitrogen compound Inorganic materials 0.000 description 1
- 150000002830 nitrogen compounds Chemical class 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 150000002897 organic nitrogen compounds Chemical class 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- BXRNXXXXHLBUKK-UHFFFAOYSA-N piperazine-2,5-dione Chemical compound O=C1CNC(=O)CN1 BXRNXXXXHLBUKK-UHFFFAOYSA-N 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 159000000001 potassium salts Chemical class 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 230000028043 self proteolysis Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- 229940074410 trehalose Drugs 0.000 description 1
- 229960004295 valine Drugs 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
- 150000003751 zinc Chemical class 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
- C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
- C07K5/06—Dipeptides
- C07K5/06104—Dipeptides with the first amino acid being acidic
- C07K5/06113—Asp- or Asn-amino acid
- C07K5/06121—Asp- or Asn-amino acid the second amino acid being aromatic or cycloaliphatic
- C07K5/0613—Aspartame
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/20—Aspartic acid; Asparagine
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/874—Pseudomonas
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/874—Pseudomonas
- Y10S435/877—Pseudomonas putida
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Description
ãçºæã®è©³çŽ°ãªèª¬æã
æ¬çºæã¯ãããã«é
žãã¢ã³ã¢ãã¢åã³ïŒ¬âããš
ãã«ã¢ã©ãã³äœçŽã¢ã«ãã«ãšã¹ãã«ãã埮çç©ã
çšããŠÎ±ââã¢ã¹ãã«ãã«ââããšãã«ã¢ã©
ãã³äœçŽã¢ã«ãã«ãšã¹ãã«ã補é ããæ¹æ³ã«é¢ã
ããã®ã§ããã
ãã«ã¢ã©ãã³äœçŽã¢ã«ãã«ãšã¹ãã«ãã埮çç©ã
çšããŠÎ±ââã¢ã¹ãã«ãã«ââããšãã«ã¢ã©
ãã³äœçŽã¢ã«ãã«ãšã¹ãã«ã補é ããæ¹æ³ã«é¢ã
ããã®ã§ããã
αââã¢ã¹ãã«ãã«ââããšãã«ã¢ã©ãã³
äœçŽã¢ã«ãã«ãšã¹ãã«ïŒä»¥äžÎ±âAPEãšäºãïŒã
ç¹ã«ã¡ãã«ãšã¹ãã«ã¯æ°ããçå³å€ãšããŠæ³šç®ã
ããŠããæçšãªç©è³ªã§ãããαâAPEã®è£œé æ³
ãšããŠã¯ãâä¿è·ïŒ¬âã¢ã¹ãã©ã®ã³é žç¡æ°Žç©ãš
âããšãã«ã¢ã©ãã³äœçŽã¢ã«ãã«ãšã¹ãã«ãå
å¿ãããŠïŒ®âä¿è·Î±âAPEãšããä¿è·åºãé€å»
ããŠÎ±âAPEãšããæ¹æ³ãâä¿è·ïŒ¬âã¢ã¹ã
ã©ã®ã³é žãšããšãã«ã¢ã©ãã³äœçŽã¢ã«ãã«ãšã¹ã
ã«ãšãèçœè³ªåè§£é µçŽ ã®ååšäžã§åå¿ãããŠïŒ®â
ä¿è·Î±âAPEããŸãâä¿è·Î±âAPEãšããšã
ã«ã¢ã©ãã³äœçŽã¢ã«ãã«ãšã¹ãã«ãšãšã®ä»å åå
ç©ãšããä¿è·åºãé€å»ããŠÎ±âAPEãšããæ¹æ³
ãªã©ãç¥ãããŠããã
äœçŽã¢ã«ãã«ãšã¹ãã«ïŒä»¥äžÎ±âAPEãšäºãïŒã
ç¹ã«ã¡ãã«ãšã¹ãã«ã¯æ°ããçå³å€ãšããŠæ³šç®ã
ããŠããæçšãªç©è³ªã§ãããαâAPEã®è£œé æ³
ãšããŠã¯ãâä¿è·ïŒ¬âã¢ã¹ãã©ã®ã³é žç¡æ°Žç©ãš
âããšãã«ã¢ã©ãã³äœçŽã¢ã«ãã«ãšã¹ãã«ãå
å¿ãããŠïŒ®âä¿è·Î±âAPEãšããä¿è·åºãé€å»
ããŠÎ±âAPEãšããæ¹æ³ãâä¿è·ïŒ¬âã¢ã¹ã
ã©ã®ã³é žãšããšãã«ã¢ã©ãã³äœçŽã¢ã«ãã«ãšã¹ã
ã«ãšãèçœè³ªåè§£é µçŽ ã®ååšäžã§åå¿ãããŠïŒ®â
ä¿è·Î±âAPEããŸãâä¿è·Î±âAPEãšããšã
ã«ã¢ã©ãã³äœçŽã¢ã«ãã«ãšã¹ãã«ãšãšã®ä»å åå
ç©ãšããä¿è·åºãé€å»ããŠÎ±âAPEãšããæ¹æ³
ãªã©ãç¥ãããŠããã
åè
ã®æ¹æ³ã¯ãâä¿è·Î±âAPEãšãšãã«ïŒ®
âä¿è·Î²âAPEãå¯çãããšããåé¡ãããã
åŸè ã®æ¹æ³ã¯ããã®ãããªåé¡ããªãç¹åã³åæ
ãšããŠã©ã»ãäœã䜿çšã§ããç¹ãªã©ã§åªããæ¹æ³
ã§ããããããããããã®æ¹æ³ã§ããåæã®ã¢ã¹
ãã©ã®ã³é žåã¯ãã®ç¡æ°Žç©ã¯ãã¢ããåºããã³ãž
ã«ãªãã·ã«ã«ããã«åºã®ãããªä¿è·åºã§ä¿è·ãã
ã®ã¡çšããå¿ èŠããã€ãã
âä¿è·Î²âAPEãå¯çãããšããåé¡ãããã
åŸè ã®æ¹æ³ã¯ããã®ãããªåé¡ããªãç¹åã³åæ
ãšããŠã©ã»ãäœã䜿çšã§ããç¹ãªã©ã§åªããæ¹æ³
ã§ããããããããããã®æ¹æ³ã§ããåæã®ã¢ã¹
ãã©ã®ã³é žåã¯ãã®ç¡æ°Žç©ã¯ãã¢ããåºããã³ãž
ã«ãªãã·ã«ã«ããã«åºã®ãããªä¿è·åºã§ä¿è·ãã
ã®ã¡çšããå¿ èŠããã€ãã
ãããã®å
¬ç¥æè¡ã§ã¯åœç¶å¿
èŠãšãããã¢ãã
åºãžã®ä¿è·åºå°å ¥åã³é€å»ã®å·¥çšã®äžå¿ èŠãªæ¹æ³
ãéçºããããšãã§ããã°å·¥çšã®ç°¡ç¥åãšããã«
䌎ãªãåæã補åçã®æ倱ãé¿ããããšãã§ãã
å·¥æ¥çã«éåžžãªå©ç¹ãçããã
åºãžã®ä¿è·åºå°å ¥åã³é€å»ã®å·¥çšã®äžå¿ èŠãªæ¹æ³
ãéçºããããšãã§ããã°å·¥çšã®ç°¡ç¥åãšããã«
䌎ãªãåæã補åçã®æ倱ãé¿ããããšãã§ãã
å·¥æ¥çã«éåžžãªå©ç¹ãçããã
æ¬çºæè
ãã¯ãâã¢ã¹ãã©ã®ã³é
žã®ä»£ãã«ã
ææ©é žã§ãããããçµæžçãªããã«é žãçšããã
ãšãã§ããã°ãããæå©ãªÎ±âAPEã®åææ³ã
確ç«ããããšèããããã«é žãšããšãã«ã¢ã©ãã³
äœçŽã¢ã«ãã«ãšã¹ãã«ããçŽæ¥Î±âAPEãåæ
ããæ¹æ³ã«ã€ããŠéææ€èšããçµæãã·ãŠãŒãã¢
ãã¹å±ã«å±ãã埮çç©ãçšããããšã«ãã€ãŠãã
ãã«é žãã¢ã³ã¢ãã¢åã³ïŒ¬âããšãã«ã¢ã©ãã³äœ
çŽã¢ã«ãã«ãšã¹ãã«ããαâAPEãçæããã
ãšãèŠåºããã
ææ©é žã§ãããããçµæžçãªããã«é žãçšããã
ãšãã§ããã°ãããæå©ãªÎ±âAPEã®åææ³ã
確ç«ããããšèããããã«é žãšããšãã«ã¢ã©ãã³
äœçŽã¢ã«ãã«ãšã¹ãã«ããçŽæ¥Î±âAPEãåæ
ããæ¹æ³ã«ã€ããŠéææ€èšããçµæãã·ãŠãŒãã¢
ãã¹å±ã«å±ãã埮çç©ãçšããããšã«ãã€ãŠãã
ãã«é žãã¢ã³ã¢ãã¢åã³ïŒ¬âããšãã«ã¢ã©ãã³äœ
çŽã¢ã«ãã«ãšã¹ãã«ããαâAPEãçæããã
ãšãèŠåºããã
æ¬çºæã¯ãã·ãŠãŒãã¢ãã¹å±ã«å±ããããã«
é žãã¢ã³ã¢ãã¢åã³ïŒ¬âããšãã«ã¢ã©ãã³äœçŽã¢
ã«ãã«ãšã¹ãã«ããαâAPEãçæãããèœå
ãæãã埮çç©ã®å¹é€ç©åã¯ãã®åŠçç©ãããã
ã«é žãã¢ã³ã¢ãã¢åã³ïŒ¬âããšãã«ã¢ã©ãã³äœçŽ
ã¢ã«ãã«ãšã¹ãã«ãšæ¥è§Šãããããšãç¹åŸŽãšãã
αââã¢ã¹ãã«ãã«ââããšãã«ã¢ã©ãã³äœ
çŽã¢ã«ãã«ãšã¹ãã«ã®è£œé æ³ãæäŸãããã®ã§ã
ãã
é žãã¢ã³ã¢ãã¢åã³ïŒ¬âããšãã«ã¢ã©ãã³äœçŽã¢
ã«ãã«ãšã¹ãã«ããαâAPEãçæãããèœå
ãæãã埮çç©ã®å¹é€ç©åã¯ãã®åŠçç©ãããã
ã«é žãã¢ã³ã¢ãã¢åã³ïŒ¬âããšãã«ã¢ã©ãã³äœçŽ
ã¢ã«ãã«ãšã¹ãã«ãšæ¥è§Šãããããšãç¹åŸŽãšãã
αââã¢ã¹ãã«ãã«ââããšãã«ã¢ã©ãã³äœ
çŽã¢ã«ãã«ãšã¹ãã«ã®è£œé æ³ãæäŸãããã®ã§ã
ãã
æ¬çºæã®åå¿ãåå¿åŒã§è¡šãããšã次ã®ããã§
ãããïŒåŒäžïŒ²ã¯äœçŽã¢ã«ãã«åºãè¡šãããïŒ æ¬çºæã§çšãã埮çç©ã¯ã·ãŠãŒãã¢ãã¹å±ã«å±
ããαâAPEçç£èã§ãããæ¬çºæè ãã«ãã€
ãŠå±±å£çæ°åéœåžã®åå£äžããåé¢ããããã®ã
ããªåŸ®çç©ã®èåŠçæ§è³ªã¯äžèšã®éãã§ããã
ãããïŒåŒäžïŒ²ã¯äœçŽã¢ã«ãã«åºãè¡šãããïŒ æ¬çºæã§çšãã埮çç©ã¯ã·ãŠãŒãã¢ãã¹å±ã«å±
ããαâAPEçç£èã§ãããæ¬çºæè ãã«ãã€
ãŠå±±å£çæ°åéœåžã®åå£äžããåé¢ããããã®ã
ããªåŸ®çç©ã®èåŠçæ§è³ªã¯äžèšã®éãã§ããã
(a) 圢æ
èæ±å¯å€©å¹å°ã§37âãïŒã24æéçè²ããå Ž
å 现èã®åœ¢åã³å€§ãã 棹è 0.5ã0.7Ã1.0ã1.5ÎŒïœ çŽ°èã®å€åœ¢æ§ã®æç¡ åèåã¯åè éåæ§ã®æç¡ æ 極 éæ¯ èåã®æç¡ ç¡ ã°ã©ã æè²æ§ é°æ§ æé žæ§ ç¡ (b) åå¹å°ã«ãããçè²ç¶æ èæ±å¯å€©å¹³æ¿å¹é€ïŒ37âãïŒæ¥éå¹é€ïŒ (ã€) ã³ãããŒåœ¢ç¶ã®é é æ®é çŽåŸçŽïŒmm (ã) ã³ãããŒã®åœ¢ å圢 (ã) ã³ãããŒè¡šé¢ã®åœ¢ç¶ å¹³æ» (ã) ã³ãããŒã®éèµ·ç¶æ åã¬ã³ãºç¶ (ã) ã³ãããŒã®åšçž å šçž (ã) ã³ãããŒã®å 容 å質 (ã) ã³ãããŒã®è²èª¿ ä¹³çœè² (ã) ã³ãããŒã®éæ床 åéæ (ãª) ã³ãããŒã®å æ²¢ éå (ã) å¯æº¶æ§è²çŽ ã®çæ å¯æº¶æ§æ·¡çžè²è²çŽ ç
æ èæ±å¯å€©æé¢å¹é€ïŒ37âãïŒæ¥éå¹é€ïŒ (ã€) çè²ã®è¯åŠ çè²è¯å¥œ (ã) ã³ãããŒã®åœ¢ å¹³æ» (ã) ã³ãããŒã®æé¢ã®éèµ·ç¶æ æå¹³ç¶ (ã) ã³ãããŒã®å æ²¢ éå (ã) ã³ãããŒè¡šé¢ã®åœ¢ç¶ å¹³æ» (ã) ã³ãããŒã®éæ床 åéæ (ã) ã³ãããŒã®è² ä¹³çœè² (ã) ã³ãããŒã®è³ª ãã¿ãŒè³ª èæ±æ¶²äœå¹é€ïŒ37âãïŒæ¥éå¹é€ïŒ (ã€) è¡šé¢ã®çè² ãªã (ã) æ¿åºŠ ããæ¿ã (ã) æ²æŸ± ç²æ«ç¶ (ã) ã¬ã¹çºç ãªã (ã) å¹å°ã®çè² ãªã èæ±å¯å€©ç©¿åºå¹é€ïŒ37âãïŒæ¥éå¹é€ïŒ (ã€) çè²ã®å Žæ äžäžäžæ§ (ã) ã³ãããŒã®åœ¢ç¶ ä¹³é ç¶ èæ±ãŒã©ãã³ç©¿åºå¹é€ïŒ20âã14æ¥éå¹
é€ïŒ (ã€) ãŒã©ãã³æ¶²å ãªã ãªããã¹ãã«ã¯ïŒ37âãïŒæ¥éå¹é€ïŒ (ã€) åå¿ BCPãéè²ã«ããªããã¹ãé玫
è²ã«ãã (ã) ååºã液å ãªã (c) ççåŠçæ§è³ª ç¡«é žå¡©ã®éå â è±çªåå¿ â MRãã¹ã â VRãã¹ã â ã€ã³ããŒã«ã®çæ â ç¡«åæ°ŽçŽ ã®çæ ïŒïŒïŒ·ïŒ ãã³ãã³ã®å æ°Žå解 â ã¯ãšã³é žã®å©çš ïŒ ç¡æ©çªçŽ æºã®å©çš ã¢ã³ã¢ãã¢æ ã®ã¿å©çš è²çŽ ã®çæ ç·é»è²æ°Žæº¶æ§èå è²çŽ çæ ãŠã¬ã¢ãŒãŒ â ãªãã·ã¿ãŒãŒ ïŒ ã«ã¿ã©ãŒãŒ ïŒ çè²ã®ç¯å² PHïŒã9.5 枩床 10ã43â é žçŽ ã«å¯Ÿããæ 床 奜æ°æ§ ïŒâãã¹ã ïŒ ç³é¡ããã®é žåã³ã¬ã¹ã®çæã®æç¡ é ž ã¬ã¹ (1) âã¢ã©ãããŒã¹ ïŒ â (2) âãã·ããŒã¹ ïŒ â (3) âã°ã«ã³ãŒã¹ ïŒ â (4) âãã³ããŒã¹ ïŒ â (5) âãã©ã¯ããŒã¹ â â (6) âã¬ã©ã¯ããŒã¹ ïŒ â (7) 麊èœç³ â â (8) ã·ãšç³ â â (9) ä¹³ ç³ â â (10) ãã¬ãããŒã¹ â â (11) âãœã«ããã â â (12) âãã³ããã â â (13) ã€ãã·ãã â â (14) ã°ãªã»ãªã³ â â (15) ãã³ãã³ â â ã¢ã«ã®ãã³ ãžãããã©ãŒãŒïŒArginine
DihydrolaseïŒ ïŒ ççŽ æºã®å©çšïŒ37âãïŒãïŒæ¥éå¹é€ïŒ å©çšãããã®ïŒïŒ€âã°ã«ã³ãŒã¹ãâããª
ã³ãβââã¢ã©ãã³ãâã¢ã«ã®ãã³ å©çšããªããã®ïŒãã¬ãããŒã¹ãã¡ãœã€ã
ã·ããŒã« ã²ã©ããªãŒã« ããŒãžã€ãºã»ããã¢ã«ã»ãªãã»ãã¿ãŒããã€ã
ã€ãã»ãã¯ããªãªããžãŒïŒBergeyâ²s Menual of
Deteminative BacteriologyïŒãïŒçïŒ1974幎ïŒ
ã®èšèŒã«åŸã€ãŠåž°å±åå®ãè¡ãªããšæ¬èã¯ã·ãŠãŒ
ãã¢ãã¹å±ã®ç¹åŸŽãæããã
å 现èã®åœ¢åã³å€§ãã 棹è 0.5ã0.7Ã1.0ã1.5ÎŒïœ çŽ°èã®å€åœ¢æ§ã®æç¡ åèåã¯åè éåæ§ã®æç¡ æ 極 éæ¯ èåã®æç¡ ç¡ ã°ã©ã æè²æ§ é°æ§ æé žæ§ ç¡ (b) åå¹å°ã«ãããçè²ç¶æ èæ±å¯å€©å¹³æ¿å¹é€ïŒ37âãïŒæ¥éå¹é€ïŒ (ã€) ã³ãããŒåœ¢ç¶ã®é é æ®é çŽåŸçŽïŒmm (ã) ã³ãããŒã®åœ¢ å圢 (ã) ã³ãããŒè¡šé¢ã®åœ¢ç¶ å¹³æ» (ã) ã³ãããŒã®éèµ·ç¶æ åã¬ã³ãºç¶ (ã) ã³ãããŒã®åšçž å šçž (ã) ã³ãããŒã®å 容 å質 (ã) ã³ãããŒã®è²èª¿ ä¹³çœè² (ã) ã³ãããŒã®éæ床 åéæ (ãª) ã³ãããŒã®å æ²¢ éå (ã) å¯æº¶æ§è²çŽ ã®çæ å¯æº¶æ§æ·¡çžè²è²çŽ ç
æ èæ±å¯å€©æé¢å¹é€ïŒ37âãïŒæ¥éå¹é€ïŒ (ã€) çè²ã®è¯åŠ çè²è¯å¥œ (ã) ã³ãããŒã®åœ¢ å¹³æ» (ã) ã³ãããŒã®æé¢ã®éèµ·ç¶æ æå¹³ç¶ (ã) ã³ãããŒã®å æ²¢ éå (ã) ã³ãããŒè¡šé¢ã®åœ¢ç¶ å¹³æ» (ã) ã³ãããŒã®éæ床 åéæ (ã) ã³ãããŒã®è² ä¹³çœè² (ã) ã³ãããŒã®è³ª ãã¿ãŒè³ª èæ±æ¶²äœå¹é€ïŒ37âãïŒæ¥éå¹é€ïŒ (ã€) è¡šé¢ã®çè² ãªã (ã) æ¿åºŠ ããæ¿ã (ã) æ²æŸ± ç²æ«ç¶ (ã) ã¬ã¹çºç ãªã (ã) å¹å°ã®çè² ãªã èæ±å¯å€©ç©¿åºå¹é€ïŒ37âãïŒæ¥éå¹é€ïŒ (ã€) çè²ã®å Žæ äžäžäžæ§ (ã) ã³ãããŒã®åœ¢ç¶ ä¹³é ç¶ èæ±ãŒã©ãã³ç©¿åºå¹é€ïŒ20âã14æ¥éå¹
é€ïŒ (ã€) ãŒã©ãã³æ¶²å ãªã ãªããã¹ãã«ã¯ïŒ37âãïŒæ¥éå¹é€ïŒ (ã€) åå¿ BCPãéè²ã«ããªããã¹ãé玫
è²ã«ãã (ã) ååºã液å ãªã (c) ççåŠçæ§è³ª ç¡«é žå¡©ã®éå â è±çªåå¿ â MRãã¹ã â VRãã¹ã â ã€ã³ããŒã«ã®çæ â ç¡«åæ°ŽçŽ ã®çæ ïŒïŒïŒ·ïŒ ãã³ãã³ã®å æ°Žå解 â ã¯ãšã³é žã®å©çš ïŒ ç¡æ©çªçŽ æºã®å©çš ã¢ã³ã¢ãã¢æ ã®ã¿å©çš è²çŽ ã®çæ ç·é»è²æ°Žæº¶æ§èå è²çŽ çæ ãŠã¬ã¢ãŒãŒ â ãªãã·ã¿ãŒãŒ ïŒ ã«ã¿ã©ãŒãŒ ïŒ çè²ã®ç¯å² PHïŒã9.5 枩床 10ã43â é žçŽ ã«å¯Ÿããæ 床 奜æ°æ§ ïŒâãã¹ã ïŒ ç³é¡ããã®é žåã³ã¬ã¹ã®çæã®æç¡ é ž ã¬ã¹ (1) âã¢ã©ãããŒã¹ ïŒ â (2) âãã·ããŒã¹ ïŒ â (3) âã°ã«ã³ãŒã¹ ïŒ â (4) âãã³ããŒã¹ ïŒ â (5) âãã©ã¯ããŒã¹ â â (6) âã¬ã©ã¯ããŒã¹ ïŒ â (7) 麊èœç³ â â (8) ã·ãšç³ â â (9) ä¹³ ç³ â â (10) ãã¬ãããŒã¹ â â (11) âãœã«ããã â â (12) âãã³ããã â â (13) ã€ãã·ãã â â (14) ã°ãªã»ãªã³ â â (15) ãã³ãã³ â â ã¢ã«ã®ãã³ ãžãããã©ãŒãŒïŒArginine
DihydrolaseïŒ ïŒ ççŽ æºã®å©çšïŒ37âãïŒãïŒæ¥éå¹é€ïŒ å©çšãããã®ïŒïŒ€âã°ã«ã³ãŒã¹ãâããª
ã³ãβââã¢ã©ãã³ãâã¢ã«ã®ãã³ å©çšããªããã®ïŒãã¬ãããŒã¹ãã¡ãœã€ã
ã·ããŒã« ã²ã©ããªãŒã« ããŒãžã€ãºã»ããã¢ã«ã»ãªãã»ãã¿ãŒããã€ã
ã€ãã»ãã¯ããªãªããžãŒïŒBergeyâ²s Menual of
Deteminative BacteriologyïŒãïŒçïŒ1974幎ïŒ
ã®èšèŒã«åŸã€ãŠåž°å±åå®ãè¡ãªããšæ¬èã¯ã·ãŠãŒ
ãã¢ãã¹å±ã®ç¹åŸŽãæããã
æŽã«çŽ°èã«ããªâβâããããã·é
ªé
žïŒpolyâ
βâhydroxybutyrateïŒã®èç©ããªãããšãèå
æ§è²çŽ ãçæããããšãã¢ã«ã®ãã³ã»ãžãããã©
ãŒãŒïŒarginine dihydrolaseïŒãååšããããšã
ãã·ãŠãŒãã¢ãã¹å±ã®ãšã¢ãã®ããµçš®ïŒP.
aeroginosaïŒããããçš®ïŒP.putidaïŒãããã¬ã»ã³
çš®ïŒP.fluoresceneïŒãã¯ãã©ãã€ã¹çš®ïŒP.
chloraphisïŒããªãŠã¬ãªãã¢ã·ãšã³ã¹çš®ïŒP.
aureofaciensïŒã®ããããã«è©²åœããããšããã
ã€ãã
βâhydroxybutyrateïŒã®èç©ããªãããšãèå
æ§è²çŽ ãçæããããšãã¢ã«ã®ãã³ã»ãžãããã©
ãŒãŒïŒarginine dihydrolaseïŒãååšããããšã
ãã·ãŠãŒãã¢ãã¹å±ã®ãšã¢ãã®ããµçš®ïŒP.
aeroginosaïŒããããçš®ïŒP.putidaïŒãããã¬ã»ã³
çš®ïŒP.fluoresceneïŒãã¯ãã©ãã€ã¹çš®ïŒP.
chloraphisïŒããªãŠã¬ãªãã¢ã·ãšã³ã¹çš®ïŒP.
aureofaciensïŒã®ããããã«è©²åœããããšããã
ã€ãã
ãããŠçè²æž©åºŠç¯å²ã¯ãããçš®ã®ãããããé«
ããšã¢ãã®ããµçš®ã«è¿ããã®ã瀺ãããç¡«é žå¡©ã
éå ããªãããšãã²ã©ããªãŒã«ãã€ãã·ããåã³
ãã¬ãããŒã¹ãè³åãããããªã³åã³Î²âã¢ã©ã
ã³ãè³åããããšãããããçš®ïŒPseudomonas
putidaïŒã®å€çš®ãšåå®ããã
ããšã¢ãã®ããµçš®ã«è¿ããã®ã瀺ãããç¡«é žå¡©ã
éå ããªãããšãã²ã©ããªãŒã«ãã€ãã·ããåã³
ãã¬ãããŒã¹ãè³åãããããªã³åã³Î²âã¢ã©ã
ã³ãè³åããããšãããããçš®ïŒPseudomonas
putidaïŒã®å€çš®ãšåå®ããã
æ¬èçš®ã®ä»£è¡šçèæ ªã§ããã·ãŠãŒãã¢ãã¹ã»ã
ããïŒPseudomonas putidaïŒTSâ15001ã¯å·¥æ¥
æè¡é¢åŸ®çç©å·¥æ¥æè¡ç 究æã«å¯èšãããŠãã
ïŒåŸ®å·¥ç æ¡å¯ç¬¬159å·ïŒã
ããïŒPseudomonas putidaïŒTSâ15001ã¯å·¥æ¥
æè¡é¢åŸ®çç©å·¥æ¥æè¡ç 究æã«å¯èšãããŠãã
ïŒåŸ®å·¥ç æ¡å¯ç¬¬159å·ïŒã
ãã®åŸ®çç©ãå¹é€ãããããªå¹å°ãšããŠã¯ãç
çŽ æºãçªçŽ æºãææ©æ é€æºãç¡æ©æ é€æºãªã©ãå«
ãéåžžã®æ é€å¹å°ã䜿çšã§ããã
çŽ æºãçªçŽ æºãææ©æ é€æºãç¡æ©æ é€æºãªã©ãå«
ãéåžžã®æ é€å¹å°ã䜿çšã§ããã
ççŽ æºãšããŠã¯ãã°ã«ã³ãŒã¹ãã·ãŠã¯ããŒã¹ã
ç³èçã®çæ°Žåç©ãªãã³ã«é ç³é žãããã«é žãã
ã¬ã€ã³é žããªã³ãŽé žçã®ææ©ç£åã³ãã®å¡©é¡ãã
çªçŽ æºãšããŠã¯éåžžã®éé µã«çšããããç¡«é žã¢ã³
ã¢ããŠã ãå¡©åã¢ã³ã¢ããŠã ãã¢ã³ã¢ãã¢ããªã³
é žã¢ã³ã¢ããŠã ãç¡é žã¢ã³ã¢ããŠã çã®ç¡æ©çªçŽ
ååç©åã³å°¿çŽ ãã³ãŒã³ã»ã¹ãã€ãŒãã»ãªã«ãŒã
ã«ãŒã€ã³ããããã³ãé µæ¯ãšãã¹ãèãšãã¹ãªã©
ã®ææ©çªçŽ ååç©ãçšããããšãã§ããã
ç³èçã®çæ°Žåç©ãªãã³ã«é ç³é žãããã«é žãã
ã¬ã€ã³é žããªã³ãŽé žçã®ææ©ç£åã³ãã®å¡©é¡ãã
çªçŽ æºãšããŠã¯éåžžã®éé µã«çšããããç¡«é žã¢ã³
ã¢ããŠã ãå¡©åã¢ã³ã¢ããŠã ãã¢ã³ã¢ãã¢ããªã³
é žã¢ã³ã¢ããŠã ãç¡é žã¢ã³ã¢ããŠã çã®ç¡æ©çªçŽ
ååç©åã³å°¿çŽ ãã³ãŒã³ã»ã¹ãã€ãŒãã»ãªã«ãŒã
ã«ãŒã€ã³ããããã³ãé µæ¯ãšãã¹ãèãšãã¹ãªã©
ã®ææ©çªçŽ ååç©ãçšããããšãã§ããã
ãã®ä»ç¡æ©æ é€æºãšããŠã¯ãäŸãã°ãã«ã«ã·ãŠ
ã å¡©ããã°ãã·ãŠã å¡©ãã«ãªãŠã å¡©ããªã³é žå¡©ã
é±å¡©ããã³ã¬ã³å¡©ãäºéå¡©ãé å¡©ãªã©ãçšããã
ãã
ã å¡©ããã°ãã·ãŠã å¡©ãã«ãªãŠã å¡©ããªã³é žå¡©ã
é±å¡©ããã³ã¬ã³å¡©ãäºéå¡©ãé å¡©ãªã©ãçšããã
ãã
ãã®åŸ®çç©ã®å¹é€ã¯æ
£çšã®æ¹æ³ã§è¡ãªãããšã
ã§ãããéåžžçŽ20ãªããçŽ40âã奜ãŸããã¯çŽ25
ãªããçŽ38âã§ãPHçŽïŒãªããçŽïŒã奜ãŸããã¯
çŽ5.5ãªããçŽ7.5ã§ãæ¯ãšããéæ°æ¹æãªã©ã®æ
段ã«ãã奜æ°çã«è¡ãªãããããªããå¹é€ã«åœã€
ãŠãå¹å°äžã«Î±âAPEãããšãã«ã¢ã©ãã³äœçŽ
ã¢ã«ãã«ãšã¹ãã«çãå°éæ·»å ããŠããããšã«ã
ã€ãŠåŸããã埮çç©ã®å¹é€ç©åã¯ãã®åŠçç©ã®Î±
âAPEçç£èœãé«ããããšãã§ããã
ã§ãããéåžžçŽ20ãªããçŽ40âã奜ãŸããã¯çŽ25
ãªããçŽ38âã§ãPHçŽïŒãªããçŽïŒã奜ãŸããã¯
çŽ5.5ãªããçŽ7.5ã§ãæ¯ãšããéæ°æ¹æãªã©ã®æ
段ã«ãã奜æ°çã«è¡ãªãããããªããå¹é€ã«åœã€
ãŠãå¹å°äžã«Î±âAPEãããšãã«ã¢ã©ãã³äœçŽ
ã¢ã«ãã«ãšã¹ãã«çãå°éæ·»å ããŠããããšã«ã
ã€ãŠåŸããã埮çç©ã®å¹é€ç©åã¯ãã®åŠçç©ã®Î±
âAPEçç£èœãé«ããããšãã§ããã
æ¬çºæã§çšãã埮çç©ã®å¹é€ç©ä»ã¯ãã®åŠçç©
ãšã¯ããã®åŸ®çç©ãå¹é€ããŠåŸãããå¹é€æ¶²ãã
ã®å¹é€æ¶²çããæ¡åããèäœãããããåŠçããŠ
åŸãæŽæµèäœã也ç¥èäœãèäœç Žç ç©ãèªå·±æ¶å
çã«ããèäœæ¶åç©ãèäœã®è¶ é³æ³¢åŠçç©ããã®
ä»ã®æº¶èçæç©åã¯ããããåºå®ãããã®ãªã©ã
äºãããŸãããããã®å¹é€ç©ããåŸãããé µçŽ è
çœè³ªåºåãå«ããã®ã§ããã
ãšã¯ããã®åŸ®çç©ãå¹é€ããŠåŸãããå¹é€æ¶²ãã
ã®å¹é€æ¶²çããæ¡åããèäœãããããåŠçããŠ
åŸãæŽæµèäœã也ç¥èäœãèäœç Žç ç©ãèªå·±æ¶å
çã«ããèäœæ¶åç©ãèäœã®è¶ é³æ³¢åŠçç©ããã®
ä»ã®æº¶èçæç©åã¯ããããåºå®ãããã®ãªã©ã
äºãããŸãããããã®å¹é€ç©ããåŸãããé µçŽ è
çœè³ªåºåãå«ããã®ã§ããã
å¹é€æ¶²ããã®èäœã®åé¢ãåŸãããèäœã®åŠç
çã¯æ £çšã®æ¹æ³ã§å®¹æã«è¡ãªãããšãã§ããã
çã¯æ £çšã®æ¹æ³ã§å®¹æã«è¡ãªãããšãã§ããã
æ¬çºæã¯ããã®åŸ®çç©ã®å¹é€ç©åã¯ãã®åŠçç©
ã氎溶液äžã§ããã«é žãã¢ã³ã¢ãã¢åã³ïŒ¬âããš
ãã«ã¢ã©ãã³äœçŽã¢ã«ãã«ãšã¹ãã«ãšæ¥è§Šããã
ããšã«ããè¡ãªããã®ã§ããããŸãæ¬çºæã¯ãçš
ãã埮çç©ã®å¹é€éäžã§ãå¹å°äžã«ããã«é žãã¢
ã³ã¢ãã¢åã³ïŒ¬âããšãã«ã¢ã©ãã³äœçŽã¢ã«ãã«
ãšã¹ãã«ãæ·»å ããŠå¹é€ãç¶ç¶ããäž¡è ãšæ¬çºæ
ã®åŸ®çç©ãšãæ¥è§Šãããããšã«ãã€ãŠãå®æœã§ã
ãã
ã氎溶液äžã§ããã«é žãã¢ã³ã¢ãã¢åã³ïŒ¬âããš
ãã«ã¢ã©ãã³äœçŽã¢ã«ãã«ãšã¹ãã«ãšæ¥è§Šããã
ããšã«ããè¡ãªããã®ã§ããããŸãæ¬çºæã¯ãçš
ãã埮çç©ã®å¹é€éäžã§ãå¹å°äžã«ããã«é žãã¢
ã³ã¢ãã¢åã³ïŒ¬âããšãã«ã¢ã©ãã³äœçŽã¢ã«ãã«
ãšã¹ãã«ãæ·»å ããŠå¹é€ãç¶ç¶ããäž¡è ãšæ¬çºæ
ã®åŸ®çç©ãšãæ¥è§Šãããããšã«ãã€ãŠãå®æœã§ã
ãã
æ¬çºæã§çšããããã«é
žãã¢ã³ã¢ãã¢åã³ïŒ¬â
ããšãã«ã¢ã©ãã³äœçŽã¢ã«ãã«ãšã¹ãã«ã¯ããã
ããéé¢ã®åœ¢ã®ãã®ã§ãã€ãŠããããããããã
ã®å¡©ã®åœ¢ã§ãã€ãŠããããããã«é žã«å¯Ÿããã¢ã³
ã¢ãã¢ã®éæ¯ã¯éåžžåœéæ¯ã§ãåè ïŒã«å¯ŸããŠåŸ
è çŽ0.5ãªããçŽ10çšåºŠã奜ãŸããã¯çŽïŒãªãã
çŽïŒã§ãããåŸã€ãŠãå®éçã«ã¯ãã®äž¡åæãšã
ãŠããã«é žã¢ã³ã¢ããŠã ïŒããã«é žæ°ŽçŽ ã¢ã³ã¢ã
ãŠã åã¯ããã«é žã¢ã³ã¢ããŠã ïŒãçšããã®ã䟿
å©ã§ããã
ããšãã«ã¢ã©ãã³äœçŽã¢ã«ãã«ãšã¹ãã«ã¯ããã
ããéé¢ã®åœ¢ã®ãã®ã§ãã€ãŠããããããããã
ã®å¡©ã®åœ¢ã§ãã€ãŠããããããã«é žã«å¯Ÿããã¢ã³
ã¢ãã¢ã®éæ¯ã¯éåžžåœéæ¯ã§ãåè ïŒã«å¯ŸããŠåŸ
è çŽ0.5ãªããçŽ10çšåºŠã奜ãŸããã¯çŽïŒãªãã
çŽïŒã§ãããåŸã€ãŠãå®éçã«ã¯ãã®äž¡åæãšã
ãŠããã«é žã¢ã³ã¢ããŠã ïŒããã«é žæ°ŽçŽ ã¢ã³ã¢ã
ãŠã åã¯ããã«é žã¢ã³ã¢ããŠã ïŒãçšããã®ã䟿
å©ã§ããã
æ¬çºæã§åŸ®çç©ã®å¹é€ç©åã¯ãã®åŠçç©ããã
ãã«é žãã¢ã³ã¢ãã¢åã³ïŒ¬âããšãã«ã¢ã©ãã³äœ
çŽã¢ã«ãã«ãšã¹ãã«ãšæ¥è§Šãããéã®æ¿åºŠã«ã¯æ Œ
å¥ã®å¶éã¯ãªãããããã«é žåã³ïŒ¬âããšãã«ã¢
ã©ãã³äœçŽã¢ã«ãã«ãšã¹ãã«ã®æ¿åºŠã¯ãéåžžãçŽ
ïŒééïŒ ãªãã溶解床ã®èš±ãç¯å²ã奜ãŸããã¯çŽ
ïŒééïŒ ãªããçŽ40ééïŒ çšåºŠã§ããã
ãã«é žãã¢ã³ã¢ãã¢åã³ïŒ¬âããšãã«ã¢ã©ãã³äœ
çŽã¢ã«ãã«ãšã¹ãã«ãšæ¥è§Šãããéã®æ¿åºŠã«ã¯æ Œ
å¥ã®å¶éã¯ãªãããããã«é žåã³ïŒ¬âããšãã«ã¢
ã©ãã³äœçŽã¢ã«ãã«ãšã¹ãã«ã®æ¿åºŠã¯ãéåžžãçŽ
ïŒééïŒ ãªãã溶解床ã®èš±ãç¯å²ã奜ãŸããã¯çŽ
ïŒééïŒ ãªããçŽ40ééïŒ çšåºŠã§ããã
æ¬çºæã®åŸ®çç©ã®å¹é€ç©åã¯ãã®åŠçç©ã®äœ¿çš
éã«ãæ Œå¥ã®å¶éã¯ãªããããããã¯éåžžã¢ã«æ¿
床ã§ããäœãæ¿åºŠã®åºè³ªïŒã¢ã«åœãã湿èééã§
çŽ10ïœãªããçŽ1000ïœã奜ãŸããã¯çŽ50ïœãªãã
çŽ500ïœã®èäœã«çžåœããå¹é€ç©åã¯åŠçç©ãçš
ããã
éã«ãæ Œå¥ã®å¶éã¯ãªããããããã¯éåžžã¢ã«æ¿
床ã§ããäœãæ¿åºŠã®åºè³ªïŒã¢ã«åœãã湿èééã§
çŽ10ïœãªããçŽ1000ïœã奜ãŸããã¯çŽ50ïœãªãã
çŽ500ïœã®èäœã«çžåœããå¹é€ç©åã¯åŠçç©ãçš
ããã
æ¬çºæã®æ¹æ³ã®åå¿æž©åºŠã¯ãéåžžçŽ10âãªãã
çŽ50âã奜ãŸããã¯çŽ25âãªããçŽ40âã§ããã
ãŸããåå¿æ¶²ã®æ¶²æ§ã¯ãPHçŽïŒãªããçŽïŒã奜ãŸ
ããã¯çŽïŒãªããçŽïŒã§ãããåŸã€ãŠãã®èª¿ç¯ã®
ããã«ç·©è¡å€ãé žåã¯å¡©åºçãé©åœã«æ·»å ããŠã
ãã
çŽ50âã奜ãŸããã¯çŽ25âãªããçŽ40âã§ããã
ãŸããåå¿æ¶²ã®æ¶²æ§ã¯ãPHçŽïŒãªããçŽïŒã奜ãŸ
ããã¯çŽïŒãªããçŽïŒã§ãããåŸã€ãŠãã®èª¿ç¯ã®
ããã«ç·©è¡å€ãé žåã¯å¡©åºçãé©åœã«æ·»å ããŠã
ãã
åå¿æéã¯äœãéå®çã§ãªãããéåžžçŽïŒæé
ãªããçŽ40æéã奜ãŸããã¯çŽ10æéãªããçŽ20
æéçšåºŠã䟿å©ã§ããã
ãªããçŽ40æéã奜ãŸããã¯çŽ10æéãªããçŽ20
æéçšåºŠã䟿å©ã§ããã
æ¬çºæã§çšããâããšãã«ã¢ã©ãã³äœçŽã¢ã«
ãã«ãšã¹ãã«ã®äœçŽã¢ã«ãã«åºã¯ãã¡ãã«åºããš
ãã«åºãã€ãœãããã«åºãªã©ã®åºã§ããã
ãã«ãšã¹ãã«ã®äœçŽã¢ã«ãã«åºã¯ãã¡ãã«åºããš
ãã«åºãã€ãœãããã«åºãªã©ã®åºã§ããã
ããšãã«ã¢ã©ãã³äœçŽã¢ã«ãã«ãšã¹ãã«ã®ïŒ€â
äœã¯å©çšãããããŸãåå¿ã«é¢äžããªãã®ã§ãã
ã®ïŒ¬âäœã«ä»£ããŠã©ã»ãäœãçšããŠãããã
äœã¯å©çšãããããŸãåå¿ã«é¢äžããªãã®ã§ãã
ã®ïŒ¬âäœã«ä»£ããŠã©ã»ãäœãçšããŠãããã
çæããαâAPEã¯å
¬ç¥ã®åé¢ç²Ÿè£œæ段ã«ã
ãåé¢ç²Ÿè£œããããšãã§ãããäŸãã°ãåå¿æ¶²ã«
èäœçã®åºåœ¢åãå«ããšãã¯ãé å¿åé¢ãéç
ã«ãããããåé¢ããã®ã¡ãå¿ èŠã«å¿ããŠé€èçœ
çã®åŠçãè¡ãªããæ £çšã®ã«ã©ã ã¯ãããã°ã©ã
ã€ãŒãèå±€ã¯ãããã°ã©ãã€ãŒãæ¶æãæ» å§äžã§
ã®ä¹Ÿç¥çã®åé¢ç²Ÿè£œæ段ã«ããαâAPEã粟補
åé¢ããããšãã§ããã
ãåé¢ç²Ÿè£œããããšãã§ãããäŸãã°ãåå¿æ¶²ã«
èäœçã®åºåœ¢åãå«ããšãã¯ãé å¿åé¢ãéç
ã«ãããããåé¢ããã®ã¡ãå¿ èŠã«å¿ããŠé€èçœ
çã®åŠçãè¡ãªããæ £çšã®ã«ã©ã ã¯ãããã°ã©ã
ã€ãŒãèå±€ã¯ãããã°ã©ãã€ãŒãæ¶æãæ» å§äžã§
ã®ä¹Ÿç¥çã®åé¢ç²Ÿè£œæ段ã«ããαâAPEã粟補
åé¢ããããšãã§ããã
æ¬çºæã«ããã°ãâã¢ã¹ãã©ã®ã³é
žãããã®
âä¿è·äœã®ä»£ãã«ããã容æã«åŸããããã€ã
ãçµæžçãªããã«é žãçšããŠãçŽã¡ã«Î±âAPE
ã補é ããããšãã§ããããŸãçååŠåå¿ãå©çš
ããã®ã§ãåæãšããŠã©ã»ãäœãçšããŠãαâ
APEã®LLâäœã®ã¿ãéžæçã«è£œé ããããšãã§
ãããæŽã«ãŸããæ¬çºæã§ã¯Î²âAPEã®å¯çã
ãªãã
âä¿è·äœã®ä»£ãã«ããã容æã«åŸããããã€ã
ãçµæžçãªããã«é žãçšããŠãçŽã¡ã«Î±âAPE
ã補é ããããšãã§ããããŸãçååŠåå¿ãå©çš
ããã®ã§ãåæãšããŠã©ã»ãäœãçšããŠãαâ
APEã®LLâäœã®ã¿ãéžæçã«è£œé ããããšãã§
ãããæŽã«ãŸããæ¬çºæã§ã¯Î²âAPEã®å¯çã
ãªãã
以äžãå®æœäŸã§æ¬çºæãæŽã«è©³çŽ°ã«èª¬æããã
å®æœäŸäžãçŸåçã¯ããããééçŸåçã瀺ãã
å®æœäŸäžãçŸåçã¯ããããééçŸåçã瀺ãã
å®æœäŸ ïŒ
ãããŒã«é
žæ°ŽçŽ ã¢ã³ã¢ããŠã ïŒïŒ
ããªã³é
žïŒæ°Ž
çŽ ïŒã«ãªãŠã 0.1ïŒ ããªã³é žïŒæ°ŽçŽ ïŒã«ãªãŠã 0.1
ïŒ ãç¡«é žãã°ãã·ãŠã ã»ïŒæ°Žå¡©0.05ïŒ ãç¡«é žç¬¬ïŒ
éã»ïŒæ°Žå¡©0.01ïŒ ãå¡©åãã³ã¬ã³0.01ïŒ ãå¡©åã
ããªãŠã 0.01ïŒ åã³æ®éšæ°Žãããªãå¹å°ïŒPH5.5ïŒ
1.0ãïŒå®¹ãããžã€ãŒåéé µæ§œã«å ¥ãã120
âã15åéæ» èãè¡ãªã€ãã
çŽ ïŒã«ãªãŠã 0.1ïŒ ããªã³é žïŒæ°ŽçŽ ïŒã«ãªãŠã 0.1
ïŒ ãç¡«é žãã°ãã·ãŠã ã»ïŒæ°Žå¡©0.05ïŒ ãç¡«é žç¬¬ïŒ
éã»ïŒæ°Žå¡©0.01ïŒ ãå¡©åãã³ã¬ã³0.01ïŒ ãå¡©åã
ããªãŠã 0.01ïŒ åã³æ®éšæ°Žãããªãå¹å°ïŒPH5.5ïŒ
1.0ãïŒå®¹ãããžã€ãŒåéé µæ§œã«å ¥ãã120
âã15åéæ» èãè¡ãªã€ãã
ããã«ãããšåäžçµæã®å¹å°ïŒPH5.5ïŒã«ã·ãŠ
ãŒãã¢ãã¹ ãããTSâ15001ã37âã§16æéå¹
é€ããŠåŸãåå¹é€æ¶²50mlãæ¥çš®ããå¹é€æéäžPH
5.5ã6.0ãç¶æããããã«2NâHCl氎溶液ã2N
âNaOH氎溶液ãæ·»å ããªããå¹é€æž©åºŠ37âã
æ¹æåšå転æ°500rpmãéæ°éïŒç©ºæ°ïŒåã®æ¡
件äžã§éæ°æ¹æå¹é€ãè¡ãªã€ãã16æéå¹é€åŸã
åŸãããå¹é€æ¶²ã®ãã¡ããã®500mlãé å¿åé¢ã
ãŠèäœãéãïŒæ¹¿æœ€èäœéïŒïœïŒããããïŒïŒ
50Mãªã³é žå¡©ç·©è¡æ¶²ïŒPH5.5ïŒ25mlã«æžæ¿ããã
ãã®æžæ¿æ¶²ãããã«é žæ°ŽçŽ ã¢ã³ã¢ããŠã 3.3ïœã
âããšãã«ã¢ã©ãã³ã¡ãã«ãšã¹ãã«4.5ïœãå«
ã氎溶液25mlã«å ããæ¯ãšãããªãã37âã§16æ
éåå¿ãè¡ãªã€ããåå¿äºçµåŸãåå¿æ¶²ã15âã
10000rpmã§30åéé å¿åé¢ããŠèäœãé€ããã
åŸãããäžæž 液ãæ°ŽïŒãšã¿ããŒã«ïŒå®¹éæ¯80ïŒ
20ïŒã溶é¢æ¶²ãšããã«ã©ã ã¯ãããã°ã©ãã€ãŒ
ïŒå å¡«å€ããšããŒã«55Fåæšãæ±æŽæ¹éå·¥æ¥(æ ª)補ïŒ
ã«ããåç»ãè¡ãªã€ããαââã¢ã¹ãã«ãã«ãŒ
âããšãã«ã¢ã©ãã³ã¡ãã«ãšã¹ãã«ã«çžåœãã
åç»åºåãæžå§äžã§æ¿çž®ãè¡ãªãçœè²ç²æ«50mgã
åŸãããã®ãã®ã®å çŽ åæå€åã³ç©çååŠçæ§è³ª
ã¯ä»¥äžã®éãã§ãã€ãã
ãŒãã¢ãã¹ ãããTSâ15001ã37âã§16æéå¹
é€ããŠåŸãåå¹é€æ¶²50mlãæ¥çš®ããå¹é€æéäžPH
5.5ã6.0ãç¶æããããã«2NâHCl氎溶液ã2N
âNaOH氎溶液ãæ·»å ããªããå¹é€æž©åºŠ37âã
æ¹æåšå転æ°500rpmãéæ°éïŒç©ºæ°ïŒåã®æ¡
件äžã§éæ°æ¹æå¹é€ãè¡ãªã€ãã16æéå¹é€åŸã
åŸãããå¹é€æ¶²ã®ãã¡ããã®500mlãé å¿åé¢ã
ãŠèäœãéãïŒæ¹¿æœ€èäœéïŒïœïŒããããïŒïŒ
50Mãªã³é žå¡©ç·©è¡æ¶²ïŒPH5.5ïŒ25mlã«æžæ¿ããã
ãã®æžæ¿æ¶²ãããã«é žæ°ŽçŽ ã¢ã³ã¢ããŠã 3.3ïœã
âããšãã«ã¢ã©ãã³ã¡ãã«ãšã¹ãã«4.5ïœãå«
ã氎溶液25mlã«å ããæ¯ãšãããªãã37âã§16æ
éåå¿ãè¡ãªã€ããåå¿äºçµåŸãåå¿æ¶²ã15âã
10000rpmã§30åéé å¿åé¢ããŠèäœãé€ããã
åŸãããäžæž 液ãæ°ŽïŒãšã¿ããŒã«ïŒå®¹éæ¯80ïŒ
20ïŒã溶é¢æ¶²ãšããã«ã©ã ã¯ãããã°ã©ãã€ãŒ
ïŒå å¡«å€ããšããŒã«55Fåæšãæ±æŽæ¹éå·¥æ¥(æ ª)補ïŒ
ã«ããåç»ãè¡ãªã€ããαââã¢ã¹ãã«ãã«ãŒ
âããšãã«ã¢ã©ãã³ã¡ãã«ãšã¹ãã«ã«çžåœãã
åç»åºåãæžå§äžã§æ¿çž®ãè¡ãªãçœè²ç²æ«50mgã
åŸãããã®ãã®ã®å çŽ åæå€åã³ç©çååŠçæ§è³ª
ã¯ä»¥äžã®éãã§ãã€ãã
å®æž¬å€ïŒïŒ
ïŒ Î±ââã¢ã¹ãã«ãã«â
âããšãã«ã¢ã©ãã³ã¡ãã«
ãšã¹ãã«ãšããŠã®èšç®å€
ïŒïŒ ïŒ ïŒ£ 57.70 57.14  6.20 6.12  10.05 9.52 èç¹ïŒ235ã236âã§ãå解ããã
âããšãã«ã¢ã©ãã³ã¡ãã«
ãšã¹ãã«ãšããŠã®èšç®å€
ïŒïŒ ïŒ ïŒ£ 57.70 57.14  6.20 6.12  10.05 9.52 èç¹ïŒ235ã236âã§ãå解ããã
æ¯æå
床ïŒãαã25/DïŒ32.0ïŒïŒ£ïŒ1.0ãé
¢é
žïŒ
ã¢ããåºãããªãããã¢ã»ãã«åãã«ã«ããã·
ã«åºãã¡ãã«åãããã®ã®ååéã¯404ã§ãã€ãã
ã«åºãã¡ãã«åãããã®ã®ååéã¯404ã§ãã€ãã
ãŸãããããâããšãã«ã¢ã©ãã«ââããš
ãã«ã¢ã©ãã³ãâããšãã«ã¢ã©ãã«ââããš
ãã«ã¢ã©ãã³ã¡ãã«ãšã¹ãã«ããžã±ãããã©ãž
ã³ãâããšãã«ã¢ã©ãã³ãâããšãã«ã¢ã©ã
ã³ã¡ãã«ãšã¹ãã«ãâã¢ã¹ãã©ã®ã³é žãâã¢
ã¹ãã©ãã«ââããšãã«ã¢ã©ãã³ãâã¢ã¹ã
ã«ãã«ââã¢ã¹ãã©ã®ã³é žãαââã¢ã¹ãã«
ãã«ââããšãã«ã¢ã©ãã³ã¡ãã«ãšã¹ãã«ãβ
ââã¢ã¹ãã«ãã«ââããšãã«ã¢ã©ãã³ãæš
æºç©è³ªãšããŠèå±€ã¯ãããã°ã©ãã€ãŒãé«é液äœ
ã¯ãããã°ã©ãã€ãŒåã³ã¢ããé žåæèšã§åæã
è¡ãªã€ããæŽã«å¡©é žã¡ã¿ããŒã«ã«ããã¡ãã«åå
ã³ããªãããã¢ã»ããŒãã¡ãã«ãšã¹ãã«ã«ããã
ãªãããã¢ã»ãã«ååŠçãè¡ãªã€ãè©Šæã®ã¬ã¹ã¯
ãããã°ã©ãã€ãŒãªãã³ã«ã¬ã¹ã¯ãããã°ã©ãã€
ãŒã»ãã¹ã¹ãã¯ãã«ã°ã©ãã€ãŒåæã«ããããã
αââã¢ã¹ãã«ãã«ââããšãã«ã¢ã©ãã³ã¡
ãã«ãšã¹ãã«ã§ããããšãåå®ç¢ºèªããã
ãã«ã¢ã©ãã³ãâããšãã«ã¢ã©ãã«ââããš
ãã«ã¢ã©ãã³ã¡ãã«ãšã¹ãã«ããžã±ãããã©ãž
ã³ãâããšãã«ã¢ã©ãã³ãâããšãã«ã¢ã©ã
ã³ã¡ãã«ãšã¹ãã«ãâã¢ã¹ãã©ã®ã³é žãâã¢
ã¹ãã©ãã«ââããšãã«ã¢ã©ãã³ãâã¢ã¹ã
ã«ãã«ââã¢ã¹ãã©ã®ã³é žãαââã¢ã¹ãã«
ãã«ââããšãã«ã¢ã©ãã³ã¡ãã«ãšã¹ãã«ãβ
ââã¢ã¹ãã«ãã«ââããšãã«ã¢ã©ãã³ãæš
æºç©è³ªãšããŠèå±€ã¯ãããã°ã©ãã€ãŒãé«é液äœ
ã¯ãããã°ã©ãã€ãŒåã³ã¢ããé žåæèšã§åæã
è¡ãªã€ããæŽã«å¡©é žã¡ã¿ããŒã«ã«ããã¡ãã«åå
ã³ããªãããã¢ã»ããŒãã¡ãã«ãšã¹ãã«ã«ããã
ãªãããã¢ã»ãã«ååŠçãè¡ãªã€ãè©Šæã®ã¬ã¹ã¯
ãããã°ã©ãã€ãŒãªãã³ã«ã¬ã¹ã¯ãããã°ã©ãã€
ãŒã»ãã¹ã¹ãã¯ãã«ã°ã©ãã€ãŒåæã«ããããã
αââã¢ã¹ãã«ãã«ââããšãã«ã¢ã©ãã³ã¡
ãã«ãšã¹ãã«ã§ããããšãåå®ç¢ºèªããã
å®æœäŸ ïŒ
ã·ãŠãŒãã¢ãã¹ ãããTSâ15001ãå®æœäŸïŒ
ãšåäžçµæã®å¹å°ã«åäžæ¡ä»¶ã§å¹é€ãããåŸãã
ãå¹é€æ¶²ã®ãã¡ããã®500mlããé å¿åé¢ã«ãã
èäœãéãïŒæ¹¿æœ€èäœéïŒïœïŒã1/50ïŒãªã³é žå¡©
ç·©è¡æ¶²25mlã«æžæ¿ãããããããŠåŸãèäœæžæ¿æ¶²
ãïŒâã§15åéè¶ é³æ³¢åŠçããŠèäœãç Žç ããã
ç Žç 液ãïŒâã10000rpmã§15åéé å¿åé¢ããŠ
åŸãäžæž 液ãããã«é žæ°ŽçŽ ã¢ã³ã¢ããŠã 3.0ïœå
ã³ïŒ¬âããšãã«ã¢ã©ãã³ã¡ãã«ãšã¹ãã«4.5ïœã
å«ã氎溶液25mlã«å ããæ¯ãšãããªãã37âã§16
æéåå¿ãè¡ãªã€ãã以äžå®æœäŸïŒãšåæ§ã«åŠç
ãè¡ãªããâã¢ã¹ãã«ãã«ââããšãã«ã¢ã©
ãã³ã¡ãã«ãšã¹ãã«ã®çœè²ç²æ«30mgãåŸãã
ãšåäžçµæã®å¹å°ã«åäžæ¡ä»¶ã§å¹é€ãããåŸãã
ãå¹é€æ¶²ã®ãã¡ããã®500mlããé å¿åé¢ã«ãã
èäœãéãïŒæ¹¿æœ€èäœéïŒïœïŒã1/50ïŒãªã³é žå¡©
ç·©è¡æ¶²25mlã«æžæ¿ãããããããŠåŸãèäœæžæ¿æ¶²
ãïŒâã§15åéè¶ é³æ³¢åŠçããŠèäœãç Žç ããã
ç Žç 液ãïŒâã10000rpmã§15åéé å¿åé¢ããŠ
åŸãäžæž 液ãããã«é žæ°ŽçŽ ã¢ã³ã¢ããŠã 3.0ïœå
ã³ïŒ¬âããšãã«ã¢ã©ãã³ã¡ãã«ãšã¹ãã«4.5ïœã
å«ã氎溶液25mlã«å ããæ¯ãšãããªãã37âã§16
æéåå¿ãè¡ãªã€ãã以äžå®æœäŸïŒãšåæ§ã«åŠç
ãè¡ãªããâã¢ã¹ãã«ãã«ââããšãã«ã¢ã©
ãã³ã¡ãã«ãšã¹ãã«ã®çœè²ç²æ«30mgãåŸãã
Claims (1)
- ãç¹èš±è«æ±ã®ç¯å²ã ïŒ ã·ãŠãŒãã¢ãã¹å±ã«å±ããããã«é žãã¢ã³ã¢
ãã¢åã³ïŒ¬âããšãã«ã¢ã©ãã³äœçŽã¢ã«ãã«ãšã¹
ãã«ããαââã¢ã¹ãã«ãã«ââããšãã«ã¢
ã©ãã³äœçŽã¢ã«ãã«ãšã¹ãã«ãçæãããããšã®
ã§ãã埮çç©ã®å¹é€ç©åã¯ãã®åŠçç©ããããã«
é žãã¢ã³ã¢ãã¢åã³ïŒ¬âããšãã«ã¢ã©ãã³äœçŽã¢
ã«ãã«ãšã¹ãã«ãšæ¥è§Šãããããšãç¹åŸŽãšããα
ââã¢ã¹ãã«ãã«ââããšãã«ã¢ã©ãã³äœçŽ
ã¢ã«ãã«ãšã¹ãã«ã®è£œé æ³ã ïŒ åå¿ãPHçŽïŒãªããçŽïŒã§è¡ãªãç¹èš±è«æ±ã®
ç¯å²ç¬¬ïŒé èšèŒã®è£œé æ³ã ïŒ åŸ®çç©ã®å¹é€ç©ããã®èäœã§ããç¹èš±è«æ±ã®
ç¯å²ç¬¬ïŒé åã¯ç¬¬ïŒé èšèŒã®è£œé æ³ã ïŒ åŸ®çç©ã®å¹é€ç©ã®åŠçç©ããã®æº¶èçæç©ã§
ããç¹èš±è«æ±ã®ç¯å²ç¬¬ïŒé åã¯ç¬¬ïŒé èšèŒã®è£œé
æ³ã ïŒ ïŒ¬âããšãã«ã¢ã©ãã³äœçŽã¢ã«ãã«ãšã¹ãã«
åã³Î±ââã¢ã¹ãã«ãã«ââããšãã«ã¢ã©ã
ã³äœçŽã¢ã«ãã«ãšã¹ãã«ã®äœçŽã¢ã«ãã«åºãã¡ã
ã«åºã§ããç¹èš±è«æ±ã®ç¯å²ç¬¬ïŒé ãªãã第ïŒé ã®
ããããã®é èšèŒã®è£œé æ³ã
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP57136332A JPS5928493A (ja) | 1982-08-06 | 1982-08-06 | ã¢ã¹ãã«ãã«ããšãã«ã¢ã©ãã³ã¢ã«ãã«ãšã¹ãã«ã®è£œé æ³ |
CA000433525A CA1201402A (en) | 1982-08-06 | 1983-07-29 | Process for preparation of aspartylphenylalanine alkyl esters |
EP83107615A EP0102529B1 (en) | 1982-08-06 | 1983-08-02 | Process for preparation of aspartylphenylalanine alkyl esters |
DE8383107615T DE3379842D1 (en) | 1982-08-06 | 1983-08-02 | Process for preparation of aspartylphenylalanine alkyl esters |
AU17565/83A AU561146B2 (en) | 1982-08-06 | 1983-08-03 | Fermentation process to produce lower alkyl esters of alpha- l-aspartyl-lphenyl-alanine |
US06/520,129 US4587214A (en) | 1982-08-06 | 1983-08-03 | Process for preparation of aspartylphenylalanine alkyl esters |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP57136332A JPS5928493A (ja) | 1982-08-06 | 1982-08-06 | ã¢ã¹ãã«ãã«ããšãã«ã¢ã©ãã³ã¢ã«ãã«ãšã¹ãã«ã®è£œé æ³ |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP15524090A Division JPH0315381A (ja) | 1990-06-15 | 1990-06-15 | æ°èŠåŸ®çç© |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5928493A JPS5928493A (ja) | 1984-02-15 |
JPH032520B2 true JPH032520B2 (ja) | 1991-01-16 |
Family
ID=15172741
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP57136332A Granted JPS5928493A (ja) | 1982-08-06 | 1982-08-06 | ã¢ã¹ãã«ãã«ããšãã«ã¢ã©ãã³ã¢ã«ãã«ãšã¹ãã«ã®è£œé æ³ |
Country Status (6)
Country | Link |
---|---|
US (1) | US4587214A (ja) |
EP (1) | EP0102529B1 (ja) |
JP (1) | JPS5928493A (ja) |
AU (1) | AU561146B2 (ja) |
CA (1) | CA1201402A (ja) |
DE (1) | DE3379842D1 (ja) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0154472B1 (en) * | 1984-03-07 | 1989-10-11 | Ajinomoto Co., Inc. | Process for the production of l-aspartyl-l-phenylalanine ester |
JPS62205781A (ja) * | 1986-03-03 | 1987-09-10 | Res Assoc Util Of Light Oil | ã·ãŠâãã¢ãã¹å±èæ ªã®å¹é€æ¹æ³ |
US7285350B2 (en) * | 2002-09-27 | 2007-10-23 | Questair Technologies Inc. | Enhanced solid oxide fuel cell systems |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3214345A (en) * | 1958-11-20 | 1965-10-26 | Tanabe Seiyaku Co | Process for producing l-aspartic acid |
GB1004218A (en) * | 1962-05-26 | 1965-09-15 | Ajinomoto Kk | A process for producing l-aspartic acid |
US3933586A (en) * | 1972-09-07 | 1976-01-20 | Les Produits Organiques Du Santerre Orsam | Method of making l-aspartic acid from fumaric acid |
US4119493A (en) * | 1975-10-23 | 1978-10-10 | (Zaidanhojin) Sagami Chemical Research Center | Process for producing a peptide |
DE2801238C2 (de) * | 1977-01-27 | 1986-06-05 | (Zaidanhojin) Sagami Chemical Research Center, Tokio/Tokyo | Verfahren zur Herstellung von Salzen aus L,L-Dipeptiden und Phenylalaninestern |
JPS5411293A (en) * | 1977-05-23 | 1979-01-27 | Toyo Soda Mfg Co Ltd | Recovery of protease |
JPS6022918B2 (ja) * | 1978-07-27 | 1985-06-04 | 財å£æ³äººçžæš¡äžå€®ç 究æ | âãã³ãžã«ãªãã·ã«ã«ããã«âïœâã¢ã¹ãã«ãã«âïœâãã§ãã«ã¢ã©ãã³ã¡ãã«âãšã¹ãã«ãšãã§ãã«ã¢ã©ãã³ã¡ãã«ãšã¹ãã«ãšã®ä»å ååç©ã®è£œé æ¹æ³ |
US4335205A (en) * | 1979-04-06 | 1982-06-15 | Greenwood James R | Low protein degradation product basal medium for identification of non-fermentative gram-negative bacilli and other microorganisms |
US4506011A (en) * | 1981-09-05 | 1985-03-19 | Toyo Soda Manufacturing Co., Ltd. | Process for preparation of aspartylphenylalanine alkyl esters |
-
1982
- 1982-08-06 JP JP57136332A patent/JPS5928493A/ja active Granted
-
1983
- 1983-07-29 CA CA000433525A patent/CA1201402A/en not_active Expired
- 1983-08-02 DE DE8383107615T patent/DE3379842D1/de not_active Expired
- 1983-08-02 EP EP83107615A patent/EP0102529B1/en not_active Expired
- 1983-08-03 US US06/520,129 patent/US4587214A/en not_active Expired - Fee Related
- 1983-08-03 AU AU17565/83A patent/AU561146B2/en not_active Ceased
Also Published As
Publication number | Publication date |
---|---|
AU1756583A (en) | 1984-02-09 |
EP0102529B1 (en) | 1989-05-10 |
DE3379842D1 (en) | 1989-06-15 |
JPS5928493A (ja) | 1984-02-15 |
EP0102529A2 (en) | 1984-03-14 |
AU561146B2 (en) | 1987-04-30 |
CA1201402A (en) | 1986-03-04 |
US4587214A (en) | 1986-05-06 |
EP0102529A3 (en) | 1986-09-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US4774179A (en) | Process for preparing a 7-aminocephalosporanic acid compound | |
US4506011A (en) | Process for preparation of aspartylphenylalanine alkyl esters | |
US3871963A (en) | Microbial protease and preparation thereof | |
US5783428A (en) | Method of producing fumaric acid | |
JPS6322188A (ja) | æ°èŠãªïœâã¢ããã¢ã·ã©â㌠| |
JPH032520B2 (ja) | ||
US5219741A (en) | Method of making L-proline using an N-acyl-L-protine acylase | |
JPH01187090A (ja) | εâããªãªã·ã³ã®è£œé æ¹æ³ããã³Îµâããªãªã·ã³çç£è | |
JPH0445159B2 (ja) | ||
FR2620731A1 (fr) | Nouveau systeme enzymatique, son procede de preparation et son application notamment dans la preparation de la d-parahydroxyphenylglycine | |
JPH01320991A (ja) | âãã¢ããšãã«ã¢ã©ãã³é¡ã®è£œé æ¹æ³ | |
EP0455170B1 (en) | Process for culturing microorganisms of the genus Pseudomonas and process for producing L-alanine using said microorganisms | |
JPS6257317B2 (ja) | ||
JPH03160995A (ja) | ãã¬ãã«ããŒã¹ã®è£œé æ³ | |
KR950005424B1 (ko) | L-ìì€í륎íž-l-íëìëŒë ë° ê·žì ëìŒí íŒíëŒì§ì ì ì¡° ë°©ë² | |
JPH0214040B2 (ja) | ||
EP0310949B1 (en) | Process for producing d-alanine | |
JPS5829078B2 (ja) | è£é µçŽ ïœâïŒâïŒã®è£œé æ³ | |
JPS6255839B2 (ja) | ||
JPH0158957B2 (ja) | ||
JPH04304894A (ja) | 埮çç©ã«ãããã©ãžã³é žã®æ°Žé žåç©ã®è£œé æ¹æ³ | |
JPS6240298A (ja) | 埮çç©åŠçã«ããå åŠæŽ»æ§ãªãžããã¢ããããâã«ã®è£œæ³ | |
JPH0362397B2 (ja) | ||
JPH0191793A (ja) | ãžâïœâãã©ã¯ãã·ã«ãã©ããŒã¹ïŒïŒïŒâ²ïŒïŒïŒïŒâ²ãžã¢ã³ãã€ãã©ã€ãã®è£œé æ³ | |
JPH07327690A (ja) | ã€ã³ãžãŽã®è£œé æ³ |