JPH0315986B2 - - Google Patents
Info
- Publication number
- JPH0315986B2 JPH0315986B2 JP18600683A JP18600683A JPH0315986B2 JP H0315986 B2 JPH0315986 B2 JP H0315986B2 JP 18600683 A JP18600683 A JP 18600683A JP 18600683 A JP18600683 A JP 18600683A JP H0315986 B2 JPH0315986 B2 JP H0315986B2
- Authority
- JP
- Japan
- Prior art keywords
- hydroxyprogesterone
- hemisuccinate
- bsa
- antigenic protein
- antigen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000004169 proteins and genes Human genes 0.000 claims description 16
- 108090000623 proteins and genes Proteins 0.000 claims description 16
- 229950000801 hydroxyprogesterone caproate Drugs 0.000 claims description 14
- DBPWSSGDRRHUNT-UHFFFAOYSA-N 17alpha-hydroxy progesterone Natural products C1CC2=CC(=O)CCC2(C)C2C1C1CCC(C(=O)C)(O)C1(C)CC2 DBPWSSGDRRHUNT-UHFFFAOYSA-N 0.000 claims description 13
- 239000000427 antigen Substances 0.000 claims description 13
- 102000036639 antigens Human genes 0.000 claims description 13
- 108091007433 antigens Proteins 0.000 claims description 13
- 230000000890 antigenic effect Effects 0.000 claims description 10
- -1 steroid compound Chemical class 0.000 claims description 7
- DOMWKUIIPQCAJU-LJHIYBGHSA-N Hydroxyprogesterone caproate Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(C)=O)(OC(=O)CCCCC)[C@@]1(C)CC2 DOMWKUIIPQCAJU-LJHIYBGHSA-N 0.000 claims 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 15
- 229940098773 bovine serum albumin Drugs 0.000 description 15
- DBPWSSGDRRHUNT-CEGNMAFCSA-N 17α-hydroxyprogesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2 DBPWSSGDRRHUNT-CEGNMAFCSA-N 0.000 description 13
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 12
- 238000000034 method Methods 0.000 description 11
- 230000009260 cross reactivity Effects 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 description 6
- 241000124008 Mammalia Species 0.000 description 4
- 241000283973 Oryctolagus cuniculus Species 0.000 description 4
- 239000002671 adjuvant Substances 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 150000002148 esters Chemical class 0.000 description 3
- 229960000890 hydrocortisone Drugs 0.000 description 3
- 230000003053 immunization Effects 0.000 description 3
- 239000000186 progesterone Substances 0.000 description 3
- 229960003387 progesterone Drugs 0.000 description 3
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 description 2
- WHBHBVVOGNECLV-OBQKJFGGSA-N 11-deoxycortisol Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 WHBHBVVOGNECLV-OBQKJFGGSA-N 0.000 description 2
- YOETUEMZNOLGDB-UHFFFAOYSA-N 2-methylpropyl carbonochloridate Chemical compound CC(C)COC(Cl)=O YOETUEMZNOLGDB-UHFFFAOYSA-N 0.000 description 2
- 241000283707 Capra Species 0.000 description 2
- 108091006905 Human Serum Albumin Proteins 0.000 description 2
- 102000008100 Human Serum Albumin Human genes 0.000 description 2
- 108010058846 Ovalbumin Proteins 0.000 description 2
- 230000001919 adrenal effect Effects 0.000 description 2
- 230000004520 agglutination Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 238000002649 immunization Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000008213 purified water Substances 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 238000003127 radioimmunoassay Methods 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- IMFACGCPASFAPR-UHFFFAOYSA-N tributylamine Chemical compound CCCCN(CCCC)CCCC IMFACGCPASFAPR-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- RWBRUCCWZPSBFC-RXRZZTMXSA-N (20S)-20-hydroxypregn-4-en-3-one Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@@H](O)C)[C@@]1(C)CC2 RWBRUCCWZPSBFC-RXRZZTMXSA-N 0.000 description 1
- JERGUCIJOXJXHF-UHFFFAOYSA-N 17alpha-Hydroxypregnenolone Natural products C1C=C2CC(O)CCC2(C)C2C1C1CCC(C(=O)C)(O)C1(C)CC2 JERGUCIJOXJXHF-UHFFFAOYSA-N 0.000 description 1
- JERGUCIJOXJXHF-TVWVXWENSA-N 17alpha-hydroxypregnenolone Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2 JERGUCIJOXJXHF-TVWVXWENSA-N 0.000 description 1
- 206010000021 21-hydroxylase deficiency Diseases 0.000 description 1
- BTJIUGUIPKRLHP-UHFFFAOYSA-N 4-nitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1 BTJIUGUIPKRLHP-UHFFFAOYSA-N 0.000 description 1
- 208000005676 Adrenogenital syndrome Diseases 0.000 description 1
- 241000700198 Cavia Species 0.000 description 1
- 208000032170 Congenital Abnormalities Diseases 0.000 description 1
- 206010010356 Congenital anomaly Diseases 0.000 description 1
- QOSSAOTZNIDXMA-UHFFFAOYSA-N Dicylcohexylcarbodiimide Chemical compound C1CCCCC1N=C=NC1CCCCC1 QOSSAOTZNIDXMA-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000283977 Oryctolagus Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- 102000014169 Steroid 21-Hydroxylase Human genes 0.000 description 1
- 108010011732 Steroid 21-Hydroxylase Proteins 0.000 description 1
- 241000473945 Theria <moth genus> Species 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000008065 acid anhydrides Chemical class 0.000 description 1
- 210000004404 adrenal cortex Anatomy 0.000 description 1
- 239000003470 adrenal cortex hormone Substances 0.000 description 1
- 125000005907 alkyl ester group Chemical group 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 150000001718 carbodiimides Chemical class 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 229960002899 hydroxyprogesterone Drugs 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000012261 overproduction Methods 0.000 description 1
- 150000002923 oximes Chemical class 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000003270 steroid hormone Substances 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP18600683A JPS6078919A (ja) | 1983-10-06 | 1983-10-06 | 17α―ヒドロキシプロゲステロンの測定用抗原 |
JP19772790A JPH03150464A (ja) | 1983-10-06 | 1990-07-27 | 17α―ヒドロキシプロゲステロン測定用抗血清 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP18600683A JPS6078919A (ja) | 1983-10-06 | 1983-10-06 | 17α―ヒドロキシプロゲステロンの測定用抗原 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP19772790A Division JPH03150464A (ja) | 1983-10-06 | 1990-07-27 | 17α―ヒドロキシプロゲステロン測定用抗血清 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS6078919A JPS6078919A (ja) | 1985-05-04 |
JPH0315986B2 true JPH0315986B2 (fr) | 1991-03-04 |
Family
ID=16180715
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP18600683A Granted JPS6078919A (ja) | 1983-10-06 | 1983-10-06 | 17α―ヒドロキシプロゲステロンの測定用抗原 |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6078919A (fr) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS62119457A (ja) * | 1985-11-19 | 1987-05-30 | Sapporo Imuno Diagnostic Lab:Kk | 先天性副腎皮質過形成のマススクリ−ニング用診断薬キツト |
-
1983
- 1983-10-06 JP JP18600683A patent/JPS6078919A/ja active Granted
Also Published As
Publication number | Publication date |
---|---|
JPS6078919A (ja) | 1985-05-04 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP0078952B1 (fr) | N-Aminoalkyldérivés d'iodothyronines, immunogènes dérivés d'iodothyronines et leurs anticorps | |
JP4990785B2 (ja) | トピラメートに関する免疫アッセイ | |
JPH07119764B2 (ja) | イオン捕捉試薬およびそれを用いた結合イムノアッセイ法 | |
EP0104527A1 (fr) | Immunogènes de la digoxigénine, anticorps, conjugués marqués et dérivés apparentés | |
JPS604422B2 (ja) | 抗原の定量方法 | |
EP0119767B1 (fr) | Procédé pour la détermination de ligands | |
JPH0428718B2 (fr) | ||
JPH02213766A (ja) | 免疫測定用試薬および免疫測定器具 | |
Stalla et al. | The development of a direct homologous radioimmunoassay for serum cortisol | |
JP2002504994A (ja) | 抗アロタイプモノクローナル抗体を使用するイムノアッセイ | |
EP0712497B1 (fr) | Reactifs et procedes de detection et de quantification de la testosterone dans des echantillons de fluides | |
EP0088974A2 (fr) | Essai immunologique homogène avec un anti-réactif marqué monoclonal | |
JPH0651670B2 (ja) | 二官能性ハプテンおよび免疫化学的ハプテン測定法 | |
JP2968910B2 (ja) | 1α,25(OH)2ビタミンD3に対する抗体及びその用途 | |
JPH0315986B2 (fr) | ||
EP0109078A1 (fr) | Essai immunochimique de la gonadotropine chorionique humaine et son réactif | |
JPH0435710B2 (fr) | ||
JPS61186855A (ja) | チロキシンを形成するグロブリンの結合能力を測定する方法 | |
JPH10265457A (ja) | インドキシル硫酸誘導体、抗原、抗体及びそれを用いるインドキシル硫酸の検出方法 | |
JPH0758291B2 (ja) | アナライトが存在可能な媒体中のアナライトをルミネセンスによって検出および/または測定するための均質方法およびその方法に使用するキット | |
JPS6073358A (ja) | 17α−ヒドロキシプロゲステロン測定用抗原及び抗血清 | |
Rao et al. | Specific antisera suitable for solid-phase radioimmunoassay of 11β-hydroxyandrost-4-ene-3, 17-dione | |
JPH06148193A (ja) | 抗リン脂質抗体結合用担体及び該担体を用いた免疫学的測定法 | |
JP3749636B2 (ja) | メダラシンの免疫学的測定方法 | |
HU194284B (en) | Process and in vitro reagent kit for the determination of primary glycol acid |