JPH02186920A - Culture of matsutake mushroom fungus - Google Patents

Abstract

PURPOSE:To culture the fungus of Matsutake mushroom in a state homogeneously dispersing the fungus in a pumice medium and in a state readily subjected to a water-retaining and controlling process after transplanted by culturing the fungus of the Matsutake mushroom in the medium comprising many pumice pieces adsorbing a culturing solution and stirring the pumice pieces. CONSTITUTION:The fungus of Matsutake mushroom is cultured in a medium comprising many pumice pieces (preferably ones having piece sizes of 3-5mm) adsorbing a culture solution and the pumice pieces are stirred to homogeneously disperse the fungus of the Matsutake mushroom in the medium, followed by preferably planting a Japanese red pine seedling in the medium to form a mycorrhiza in the seedling.

Description

[Detailed description of the invention] (Industrial use) This invention relates to the cultivation of mushrooms, matsutake,
It is especially used for culturing matsutake mushroom starter and transplanting the starter.

(Conventional technology) Conventionally, sawdust is generally used as a culture medium for matsutake fungi, but the above bacteria grows on the surface of the medium such as sawdust, connects, forms clumps, and propagates into the inner layer of the medium. It was not possible to achieve uniform growth throughout the medium.

(Problems to be Solved by the Invention) In order to prevent the formation of conventional boundaries, the present invention aims to divide the boundaries into smaller sections and disperse them evenly throughout the culture medium.

(Means for Solving the Problems) In order to achieve the above object, this invention places a multi-porous stone or pumice stone in a culture bottle to adsorb a culture solution, and inoculates it with matsutake fungi to grow the matsutake fungus. After culturing and growing, the pumice stones on which Matsutake fungi have grown are individually and evenly dispersed in the pumice culture medium by shaking the culture bottle.

(Effect) Matsutake mushroom seeds are evenly distributed throughout the pumice stone medium. As a result, the growth of the inoculum in the pumice medium becomes uniform, and subsequent transplantation to red pine seedlings can be easily performed using pumice.

(Example λ Preparation of culture solution Add the trade name Ebios 59 to fresh water too mt, and add 1
Heat leaching at 20C for 60 minutes, then filter. Add 1 part of Amino Ugly (Strong Camoly Amino S Injection manufactured by Morishita Pharmaceutical Co., Ltd.) to this exudate.
Add 5 to 20 ml of glucose and 12 rnl of 1N hydrochloric acid solution to prepare a culture solution.

Culture using pumice medium Place a 3-5M dry pumice stone 909 in a transparent bottle with a diameter and height of about 8 (maximum), and add 15QmJ of the above culture solution to it.
Add, stopper, and sterilize by heating at 1200℃ for 60 minutes. The pH will be around 5. The remaining culture fluid absorbed by the pumice stones accumulates at the bottom and serves to prevent drying during the cultivation period. After the above-mentioned sterilization, the matsutake fungi that had been previously cultured on the agar slant medium are inoculated onto the pumice medium and cultured at 240 ml. After about one row, matsutake fungi grow in a portion of the medium. Then, shake the bottle vigorously (shake it to mix it evenly, and then culture it for 2 to 6 months at 240° C.). The Matsutake fungus will develop in a white color throughout the pumice stone medium. Culture it for 6 to 4 months without shaking as described above. However, when culturing in large quantities or in a large number of bottles, the above-mentioned culture is used as a starter.

In this case, the culture period can be shortened.

The pumice stones on which the cultured transplanted bacteria have grown sufficiently are taken out and placed in unglazed or synthetic resin flower pots (9 to 12 cs in diameter), and 1 to 2 year old red pine seedlings are planted therein. Also use soil such as Akadama. Pour water with as little bacteria as possible, and water once the surface of the medium is dry.

Product name Hybonex 1. Add 1% glucose once or twice a month. ooo Absorb the double solution. After one to two years, red pine seedlings begin to form mycorrhizas.

If mycorrhizae have not formed or are insufficient, wash the red pine roots and replant on a pumice layer where cultured bacteria have grown. Pumice stones with a size of 6 to 5 mm are suitable for uniform mixing when placed in a bottle and shaken. Potted pumice culture does not interfere with the growth of red pine seedlings by providing water, and there is no harm in leaving them in a frozen state.

(Effects of the Invention) By using pumice, the matsutake fungus that grows can be evenly dispersed in the pumice culture medium, and transplantation is simplified, and water retention management after transplantation is also facilitated.

Claims (3)

[Claims] (1) A method for culturing matsutake fungi using pumice, which is characterized by culturing matsutake fungi in a medium consisting of a large number of pumice stones that have adsorbed a culture solution, and stirring the pumice stones to evenly disperse the matsutake fungi in the medium. (2) The method for culturing Matsutake fungi using pumice as set forth in claim (1), wherein the pumice consists of grains of 3 to 5 mm. (3) Cultivate matsutake fungi in a medium consisting of a large number of pumice stones that have adsorbed the culture solution, and plant red pine seedlings in the medium in which the matsutake seed fungi are evenly distributed by stirring the pumice stones, and form mycorrhizae on the seedlings using pumice stones. Matsutake mushroom culture method.