JPH01285187A - クラス２制限エンドヌクレアーゼ及びその製法 - Google Patents

クラス２制限エンドヌクレアーゼ及びその製法

Info

Publication number
JPH01285187A
JPH01285187A JP1081667A JP8166789A JPH01285187A JP H01285187 A JPH01285187 A JP H01285187A JP 1081667 A JP1081667 A JP 1081667A JP 8166789 A JP8166789 A JP 8166789A JP H01285187 A JPH01285187 A JP H01285187A
Authority
JP
Japan
Prior art keywords
restriction endonuclease
class
achromobacter
recognition sequence
labeling
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP1081667A
Other languages
English (en)
Japanese (ja)
Other versions
JPH0422554B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html
Inventor
Bryan John Bolton
ブライアン・ジヨン・ボルトン
Michael Jarsch
ミヒヤエル・ヤルシユ
Gudrun Schmitz
グーン・シユミツツ
Christoph Kessler
クリストフ・ケスラー
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of JPH01285187A publication Critical patent/JPH01285187A/ja
Publication of JPH0422554B2 publication Critical patent/JPH0422554B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
JP1081667A 1988-04-02 1989-04-03 クラス２制限エンドヌクレアーゼ及びその製法 Granted JPH01285187A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19883811279 DE3811279A1 (de) 1988-04-02 1988-04-02 Typ ii-restriktionsendonuklease aspi
DE3811279.5 1988-04-02

Publications (2)

Publication Number Publication Date
JPH01285187A true JPH01285187A (ja) 1989-11-16
JPH0422554B2 JPH0422554B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 1992-04-17

Family

ID=6351328

Family Applications (1)

Application Number Title Priority Date Filing Date
JP1081667A Granted JPH01285187A (ja) 1988-04-02 1989-04-03 クラス２制限エンドヌクレアーゼ及びその製法

Country Status (3)

Country Link
EP (1) EP0336285A3 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
JP (1) JPH01285187A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
DE (1) DE3811279A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)

Also Published As

Publication number Publication date
DE3811279C2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 1990-03-01
EP0336285A3 (de) 1990-10-10
EP0336285A2 (de) 1989-10-11
JPH0422554B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 1992-04-17
DE3811279A1 (de) 1989-10-12

Similar Documents

Publication Publication Date Title
Sjöberg et al. Overproduction and purification of the B2 subunit of ribonucleotide reductase from Escherichia coli.
Neddermann et al. The purification of a mismatch-specific thymine-DNA glycosylase from HeLa cells.
US5192676A (en) Type ii restriction endonuclease, asci, obtainable from arthrobacter species and a process for producing the same
US5061628A (en) Restriction endonuclease FseI
JPH10309192A (ja) 耐熱性ジアホラーゼ遺伝子
JPH01285187A (ja) クラス２制限エンドヌクレアーゼ及びその製法
US6238904B1 (en) Type II restriction endonuclease, HpyCH4III, obtainable from Helicobacter pylori CH4 and a process for producing the same
JPS62126972A (ja) 新規制限酵素及びその製造法
US5158878A (en) Type ii restriction endonuclease swai
Chmuzh et al. A Novel Restriction Endonuclease BisI from Bacillus subtilis T30 Recognizes a Methylated DNA Sequence 5'-G (m5C)↓ NGC-3'
JPH04258290A (ja) Ｉｉ型−制限エンドヌクレーアーゼ及びこれを取得する方法並びに相補的２本鎖ｄｎａ−配列を認識しかつ切断する方法
Zeng et al. Defective DNA topoisomerase I activity in a DNAts mutant of Balb/3T3 cells
JPH029373A (ja) ＡｓｅＩ制限エンドヌクレアーゼとメチラーゼの製造方法
HU215231B (hu) Géntechnológiai eljárás S-(+)-2,2-dimetil-ciklopropán-karboxamid előállítására mikroorganizmusok segítségével
JPH0213373A (ja) クラス２制限エンドヌクレアーゼ、その製造方法および相補的二重鎖ｄｎａ配列の認識および切断方法
JPH03172178A (ja) ２型―制限エンドヌクレアーゼＳｇｒＡＩ
US5153122A (en) Type ii restriction endonuclease mami
JP2923771B1 (ja) アミノトランスフェラーゼ活性を有する耐熱性酵素及びそれをコードする遺伝子
JPH03172179A (ja) ２型―制限エンドヌクレアーゼＭｃｒＩ
JPH0458884A (ja) 新規制限酵素
JP2801263B2 (ja) カルバメート‐ヒドロラーゼを単離し引続き精製する方法、カルバメート‐ヒドロラーゼのＢｒＣＮ‐分解ペプチド、合成オリゴヌクレオチドの製法、カルバメート‐ヒドロラーゼ‐遺伝子の単離法及びアルトロバクター・オキシダンスの新規菌株
JPH0422553B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
US5470732A (en) Restriction enzyme from Brevibacterium linens
JP3498806B2 (ja) 新規制限酵素
US4724209A (en) Process for producing restriction enzyme