JP7801770B2 - 三次元（３ｄ）組織培養用のサポーティングバス - Google Patents

三次元（３ｄ）組織培養用のサポーティングバス

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Publication number
JP7801770B2
JP7801770B2 JP2022510781A JP2022510781A JP7801770B2 JP 7801770 B2 JP7801770 B2 JP 7801770B2 JP 2022510781 A JP2022510781 A JP 2022510781A JP 2022510781 A JP2022510781 A JP 2022510781A JP 7801770 B2 JP7801770 B2 JP 7801770B2
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Prior art keywords
gel
bath
polymer
dimensional tissue
polysaccharide
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JP2022510781A
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Japanese (ja)
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JPWO2021193981A1 (https=
Inventor
典弥 松▲崎▼
ドンヒ カン
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University of Osaka NUC
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Osaka University NUC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/30—Constructional details, e.g. recesses, hinges biodegradable
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Developmental Biology & Embryology (AREA)
  • Sustainable Development (AREA)
  • Pregnancy & Childbirth (AREA)
  • Dermatology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2022510781A 2020-03-26 2021-03-25 三次元（３ｄ）組織培養用のサポーティングバス Active JP7801770B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020056029 2020-03-26
JP2020056029 2020-03-26
PCT/JP2021/014105 WO2021193981A1 (ja) 2020-03-26 2021-03-25 三次元（３ｄ）組織培養用のサポーティングバス

Publications (2)

Publication Number Publication Date
JPWO2021193981A1 JPWO2021193981A1 (https=) 2021-09-30
JP7801770B2 true JP7801770B2 (ja) 2026-01-19

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JP2022510781A Active JP7801770B2 (ja) 2020-03-26 2021-03-25 三次元（３ｄ）組織培養用のサポーティングバス

Country Status (4)

Country Link
US (1) US20230105586A1 (https=)
EP (1) EP4130235A4 (https=)
JP (1) JP7801770B2 (https=)
WO (1) WO2021193981A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114854042A (zh) * 2022-05-07 2022-08-05 暨南大学 一种用于悬浮3d生物打印的悬浮介质及制备方法与应用
CN115353726B (zh) * 2022-05-16 2023-08-08 浙江大学 支持浴材料及其制备方法和组合
JPWO2024014377A1 (https=) * 2022-07-12 2024-01-18
CN116572524B (zh) * 2023-05-17 2024-07-19 宁波大学 卡拉胶在生物3d打印中的应用

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001525826A (ja) 1997-05-16 2001-12-11 アムジエン・インコーポレーテツド 持続放出性遅延型ゲル
JP2016039798A (ja) 2014-08-12 2016-03-24 旭化成ケミカルズ株式会社 ピックル液
WO2017141691A1 (ja) 2016-02-16 2017-08-24 国立大学法人大阪大学 脈管系構造を有する三次元組織の製造方法、および脈管系構造のゲルを含む三次元組織
WO2018191244A1 (en) 2017-04-10 2018-10-18 TheWell Bioscience Hydrogel for cell culture and biomedical applications
JP2019041755A (ja) 2017-09-04 2019-03-22 学校法人早稲田大学 チキソトロピー性を有するゲルを用いる多層３次元細胞培養足場システム
US20190249134A1 (en) 2016-03-15 2019-08-15 Boston Biomedical, Inc. Cell cultures and use thereof
WO2019155391A1 (en) 2018-02-06 2019-08-15 Regen Lab Sa Cross-linked hyaluronic acids and combinations with prp/bmc

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6762936B2 (ja) * 2014-12-11 2020-09-30 イーティーエッチ チューリッヒ 軟骨修復のための移植片足場及びその製造方法
WO2017049066A1 (en) 2015-09-18 2017-03-23 University Of Florida Research Foundation, Incorporated Apparatus for culturing and interacting with a three-dimensional cell culture
JP7177045B2 (ja) * 2016-06-03 2022-11-22 ビコ グループ アー・ベー 化粧品の試験及び移植のためのモデルとして使用するための、新規のプリンティングヘッドを用いたヒト皮膚の３ｄバイオプリンティングのための、フィブリン含有又は非含有のｒｇｄ接合多糖類バイオインクの調製及び適用
WO2019199899A1 (en) * 2018-04-09 2019-10-17 Case Western Reserve University Bioink and crosslinkable support medium for printing

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001525826A (ja) 1997-05-16 2001-12-11 アムジエン・インコーポレーテツド 持続放出性遅延型ゲル
JP2016039798A (ja) 2014-08-12 2016-03-24 旭化成ケミカルズ株式会社 ピックル液
WO2017141691A1 (ja) 2016-02-16 2017-08-24 国立大学法人大阪大学 脈管系構造を有する三次元組織の製造方法、および脈管系構造のゲルを含む三次元組織
US20190249134A1 (en) 2016-03-15 2019-08-15 Boston Biomedical, Inc. Cell cultures and use thereof
WO2018191244A1 (en) 2017-04-10 2018-10-18 TheWell Bioscience Hydrogel for cell culture and biomedical applications
JP2019041755A (ja) 2017-09-04 2019-03-22 学校法人早稲田大学 チキソトロピー性を有するゲルを用いる多層３次元細胞培養足場システム
WO2019155391A1 (en) 2018-02-06 2019-08-15 Regen Lab Sa Cross-linked hyaluronic acids and combinations with prp/bmc

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Science Advances，2015年，Vol.1，e1500758

Also Published As

Publication number Publication date
US20230105586A1 (en) 2023-04-06
WO2021193981A1 (ja) 2021-09-30
JPWO2021193981A1 (https=) 2021-09-30
EP4130235A4 (en) 2024-07-10
EP4130235A1 (en) 2023-02-08

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