JP7772363B2 - メイタンシノールの酵素的生産方法 - Google Patents

メイタンシノールの酵素的生産方法

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Publication number
JP7772363B2
JP7772363B2 JP2021561569A JP2021561569A JP7772363B2 JP 7772363 B2 JP7772363 B2 JP 7772363B2 JP 2021561569 A JP2021561569 A JP 2021561569A JP 2021561569 A JP2021561569 A JP 2021561569A JP 7772363 B2 JP7772363 B2 JP 7772363B2
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JP
Japan
Prior art keywords
amino acid
acid sequence
positions
sequence corresponding
seq
Prior art date
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Active
Application number
JP2021561569A
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English (en)
Japanese (ja)
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JPWO2021107133A1 (https=
Inventor
祐太朗 馬場
賢治 西村
佑太 三木
志慧 川▲崎▼
剛 山本
真由 川▲崎▼
祥吾 中野
創平 伊藤
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Microbiopharm Japan Co Ltd
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Microbiopharm Japan Co Ltd
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Publication of JPWO2021107133A1 publication Critical patent/JPWO2021107133A1/ja
Application granted granted Critical
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/188—Heterocyclic compound containing in the condensed system at least one hetero ring having nitrogen atoms and oxygen atoms as the only ring heteroatoms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01001—Carboxylesterase (3.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
JP2021561569A 2019-11-29 2020-11-27 メイタンシノールの酵素的生産方法 Active JP7772363B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019217460 2019-11-29
JP2019217460 2019-11-29
PCT/JP2020/044347 WO2021107133A1 (ja) 2019-11-29 2020-11-27 メイタンシノールの酵素的生産方法

Publications (2)

Publication Number Publication Date
JPWO2021107133A1 JPWO2021107133A1 (https=) 2021-06-03
JP7772363B2 true JP7772363B2 (ja) 2025-11-18

Family

ID=76129659

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2021561569A Active JP7772363B2 (ja) 2019-11-29 2020-11-27 メイタンシノールの酵素的生産方法

Country Status (4)

Country Link
US (1) US12473579B2 (https=)
EP (1) EP4067497A4 (https=)
JP (1) JP7772363B2 (https=)
WO (1) WO2021107133A1 (https=)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002016368A1 (en) 2000-08-18 2002-02-28 Immunogen, Inc. Process for the preparation and purification of thiol-containing maytansinoids
WO2011102388A1 (ja) 2010-02-16 2011-08-25 株式会社エーピーアイ コーポレーション １－アミノ－１－アルコキシカルボニル－２－ビニルシクロプロパンの製造方法
WO2012029819A1 (ja) 2010-08-31 2012-03-08 株式会社エーピーアイ コーポレーション 新規加水分解酵素タンパク質

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5529972A (en) 1978-08-24 1980-03-03 Takeda Chem Ind Ltd Preparation of maytansinol
JPS61123719A (ja) 1984-11-19 1986-06-11 Mazda Motor Corp タ−ボ過給機の潤滑装置
US20020156274A1 (en) 2001-03-16 2002-10-24 Terfloth Gerald J. Process for preparing maytansinol
EP1945647B9 (en) 2005-11-08 2012-11-14 Immunogen, Inc. Processes for preparation of maytansinol
ES2327480B1 (es) 2007-06-15 2010-08-10 Bioiberica, S.A. "disacaridos para el tratamiento de tendones, ligamentos y huesos".
LT3486248T (lt) 2012-09-26 2021-08-25 Immunogen, Inc. Patobulinti maitansinolio acilinimo būdai

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002016368A1 (en) 2000-08-18 2002-02-28 Immunogen, Inc. Process for the preparation and purification of thiol-containing maytansinoids
WO2011102388A1 (ja) 2010-02-16 2011-08-25 株式会社エーピーアイ コーポレーション １－アミノ－１－アルコキシカルボニル－２－ビニルシクロプロパンの製造方法
WO2012029819A1 (ja) 2010-08-31 2012-03-08 株式会社エーピーアイ コーポレーション 新規加水分解酵素タンパク質

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ZOCK J., et al.，The Bacillus subtilis pnbA gene encoding p-nitrobenzyl esterase: cloning, sequence and high-level expression in Escherichia coli，Gene，1994年，Vol.151，pp.37-43

Also Published As

Publication number Publication date
JPWO2021107133A1 (https=) 2021-06-03
EP4067497A1 (en) 2022-10-05
WO2021107133A1 (ja) 2021-06-03
US20230030526A1 (en) 2023-02-02
EP4067497A4 (en) 2024-03-13
US12473579B2 (en) 2025-11-18
WO2021107133A8 (ja) 2021-09-30

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