JP7756602B2 - 遺伝子分析方法、遺伝子分析装置、及び遺伝子分析用キット - Google Patents

遺伝子分析方法、遺伝子分析装置、及び遺伝子分析用キット

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Publication number
JP7756602B2
JP7756602B2 JP2022097908A JP2022097908A JP7756602B2 JP 7756602 B2 JP7756602 B2 JP 7756602B2 JP 2022097908 A JP2022097908 A JP 2022097908A JP 2022097908 A JP2022097908 A JP 2022097908A JP 7756602 B2 JP7756602 B2 JP 7756602B2
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Japan
Prior art keywords
substrate
fluorescent dye
extension reaction
fluorescent
base extension
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JP2022097908A
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English (en)
Japanese (ja)
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JP2023184021A5 (enExample
JP2023184021A (ja
Inventor
貴洋 安藤
崇秀 横井
千裕 万里
隆 穴沢
祥子 川上
猛 石田
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Hitachi Ltd
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Hitachi Ltd
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Priority to JP2022097908A priority Critical patent/JP7756602B2/ja
Priority to US18/857,536 priority patent/US20250341468A1/en
Priority to PCT/JP2023/006013 priority patent/WO2023243147A1/ja
Publication of JP2023184021A publication Critical patent/JP2023184021A/ja
Publication of JP2023184021A5 publication Critical patent/JP2023184021A5/ja
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/12—Circuits of general importance; Signal processing
    • G01N2201/125—Digital circuitry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Electrochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Optics & Photonics (AREA)
  • Medicinal Chemistry (AREA)
  • Sustainable Development (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2022097908A 2022-06-17 2022-06-17 遺伝子分析方法、遺伝子分析装置、及び遺伝子分析用キット Active JP7756602B2 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2022097908A JP7756602B2 (ja) 2022-06-17 2022-06-17 遺伝子分析方法、遺伝子分析装置、及び遺伝子分析用キット
US18/857,536 US20250341468A1 (en) 2022-06-17 2023-02-20 Gene analysis method, gene analysis apparatus, and gene analysis kit
PCT/JP2023/006013 WO2023243147A1 (ja) 2022-06-17 2023-02-20 遺伝子分析方法、遺伝子分析装置、及び遺伝子分析用キット

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2022097908A JP7756602B2 (ja) 2022-06-17 2022-06-17 遺伝子分析方法、遺伝子分析装置、及び遺伝子分析用キット

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JP2023184021A JP2023184021A (ja) 2023-12-28
JP2023184021A5 JP2023184021A5 (enExample) 2024-10-28
JP7756602B2 true JP7756602B2 (ja) 2025-10-20

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US (1) US20250341468A1 (enExample)
JP (1) JP7756602B2 (enExample)
WO (1) WO2023243147A1 (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2025007748A (ja) * 2023-07-03 2025-01-17 株式会社日立製作所 遺伝子変異の定量分析方法、定量分析装置、及び定量分析キット
JP2025073334A (ja) 2023-10-26 2025-05-13 Ｔｏｐｐａｎホールディングス株式会社 受電機、無線給電システム、及び、無線給電方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003310300A (ja) 2002-04-24 2003-11-05 Hitachi Ltd 遺伝子検査方法
JP2004526423A (ja) 2000-12-01 2004-09-02 コーネル リサーチ ファンデーション インコーポレーテッド 結合エンドヌクレアーゼ開裂および連結反応を使用した核酸の相違の検出方法
JP2006508632A (ja) 2002-03-01 2006-03-16 ラブジェン， インコーポレイテッド 遺伝子疾患の検出法
JP2006521086A (ja) 2003-02-28 2006-09-21 ラブジェン， インコーポレイテッド 遺伝子疾患の検出方法
CN1982470A (zh) 2005-12-13 2007-06-20 广州华银医药科技有限公司 一种同时检测靶基因序列及已知点突变的新技术
JP2016189704A (ja) 2015-03-31 2016-11-10 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5723993B2 (ja) * 2012-10-19 2015-05-27 株式会社日立製作所 遺伝子分析方法および遺伝子分析装置および分析用キット

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004526423A (ja) 2000-12-01 2004-09-02 コーネル リサーチ ファンデーション インコーポレーテッド 結合エンドヌクレアーゼ開裂および連結反応を使用した核酸の相違の検出方法
JP2006508632A (ja) 2002-03-01 2006-03-16 ラブジェン， インコーポレイテッド 遺伝子疾患の検出法
JP2003310300A (ja) 2002-04-24 2003-11-05 Hitachi Ltd 遺伝子検査方法
JP2006521086A (ja) 2003-02-28 2006-09-21 ラブジェン， インコーポレイテッド 遺伝子疾患の検出方法
CN1982470A (zh) 2005-12-13 2007-06-20 广州华银医药科技有限公司 一种同时检测靶基因序列及已知点突变的新技术
JP2016189704A (ja) 2015-03-31 2016-11-10 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
福島 弘文，蛍光標識プライマーを用いたSTR多型分析，Jpn. J. Electroph.，1997年，Vol.41, No.6，p.301-305

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US20250341468A1 (en) 2025-11-06
WO2023243147A1 (ja) 2023-12-21
JP2023184021A (ja) 2023-12-28

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