JP7744244B2 - 翻訳用組成物及びペプチドの製造方法 - Google Patents

翻訳用組成物及びペプチドの製造方法

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Publication number
JP7744244B2
JP7744244B2 JP2021567657A JP2021567657A JP7744244B2 JP 7744244 B2 JP7744244 B2 JP 7744244B2 JP 2021567657 A JP2021567657 A JP 2021567657A JP 2021567657 A JP2021567657 A JP 2021567657A JP 7744244 B2 JP7744244 B2 JP 7744244B2
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Prior art keywords
trna
anticodon
base
seq
ile
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JP2021567657A
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Japanese (ja)
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JPWO2021132546A1 (https=
JPWO2021132546A5 (https=
Inventor
万奈 籠谷
博子 山下
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Chugai Pharmaceutical Co Ltd
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Chugai Pharmaceutical Co Ltd
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Priority claimed from PCT/JP2019/051241 external-priority patent/WO2020138336A1/ja
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Publication of JPWO2021132546A5 publication Critical patent/JPWO2021132546A5/ja
Priority to JP2025151312A priority Critical patent/JP2025172162A/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
JP2021567657A 2019-12-26 2020-12-25 翻訳用組成物及びペプチドの製造方法 Active JP7744244B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2025151312A JP2025172162A (ja) 2019-12-26 2025-09-11 翻訳用組成物及びペプチドの製造方法

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JPPCT/JP2019/051241 2019-12-26
PCT/JP2019/051241 WO2020138336A1 (ja) 2018-12-26 2019-12-26 コドン拡張のための変異ｔＲＮＡ
JP2020109292 2020-06-25
JP2020109292 2020-06-25
PCT/JP2020/048652 WO2021132546A1 (ja) 2019-12-26 2020-12-25 翻訳用組成物及びペプチドの製造方法

Related Child Applications (1)

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JP2025151312A Division JP2025172162A (ja) 2019-12-26 2025-09-11 翻訳用組成物及びペプチドの製造方法

Publications (3)

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JPWO2021132546A1 JPWO2021132546A1 (https=) 2021-07-01
JPWO2021132546A5 JPWO2021132546A5 (https=) 2023-12-08
JP7744244B2 true JP7744244B2 (ja) 2025-09-25

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JP2021567657A Active JP7744244B2 (ja) 2019-12-26 2020-12-25 翻訳用組成物及びペプチドの製造方法
JP2025151312A Pending JP2025172162A (ja) 2019-12-26 2025-09-11 翻訳用組成物及びペプチドの製造方法

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JP2025151312A Pending JP2025172162A (ja) 2019-12-26 2025-09-11 翻訳用組成物及びペプチドの製造方法

Country Status (6)

Country Link
US (1) US20230108274A1 (https=)
EP (1) EP4026906A4 (https=)
JP (2) JP7744244B2 (https=)
KR (1) KR20220121831A (https=)
CN (1) CN114641569A (https=)
WO (1) WO2021132546A1 (https=)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW202446781A (zh) 2011-12-28 2024-12-01 日商中外製藥股份有限公司 胜肽化合物之製造方法
EP4166664A1 (en) 2015-03-13 2023-04-19 Chugai Seiyaku Kabushiki Kaisha Modified aminoacyl-trna synthetase and use thereof
JP7187323B2 (ja) 2017-01-31 2022-12-12 中外製薬株式会社 無細胞翻訳系におけるペプチドの合成方法
KR20250007021A (ko) 2017-06-09 2025-01-13 추가이 세이야쿠 가부시키가이샤 N-치환 아미노산을 포함하는 펩타이드의 합성 방법
EP3878836B1 (en) 2018-11-07 2025-07-23 Chugai Seiyaku Kabushiki Kaisha O-substituted serine derivative production method
EP3939962A4 (en) 2019-03-15 2022-12-28 Chugai Seiyaku Kabushiki Kaisha PROCESS FOR PREPARING AN AROMATIC AMINO ACID DERIVATIVE
BR112022007535A2 (pt) 2019-11-07 2022-07-12 Chugai Pharmaceutical Co Ltd Composto peptídico cíclico tendo ação inibitória de kras
JP7823181B2 (ja) 2022-05-06 2026-03-03 中外製薬株式会社 Ｈｒａｓおよびｎｒａｓに対して選択的なｋｒａｓ阻害作用を有する環状化合物

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014217352A (ja) 2013-05-10 2014-11-20 国立大学法人東京大学 ペプチドライブラリの製造方法、ペプチドライブラリ、及びスクリーニング方法
JP2017063672A (ja) 2015-09-29 2017-04-06 国立大学法人 東京大学 タンパク質を製造するための翻訳系、及びタンパク質の製造方法
WO2019077887A1 (ja) 2017-10-16 2019-04-25 国立大学法人東京大学 Ｄ－アミノ酸及びβ－アミノ酸の取り込みを増強するｔＲＮＡのＤ及びＴアームの改変

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JP2006500927A (ja) * 2002-09-13 2006-01-12 ザ・ユニバーシティ・オブ・クイーンズランド コドン翻訳効率に基づく遺伝子発現系
WO2006135096A1 (ja) 2005-06-14 2006-12-21 Gifu University 部位特異的にタンパク質にチロシンアナログを導入する方法
WO2007061136A1 (ja) 2005-11-24 2007-05-31 Riken 非天然型アミノ酸を組み込んだタンパク質の製造方法
WO2007066627A1 (ja) 2005-12-06 2007-06-14 The University Of Tokyo 多目的アシル化触媒とその用途
AU2007224019A1 (en) 2006-03-03 2007-09-13 California Institute Of Technology Site-specific incorporation of amino acids into molecules
WO2008001947A1 (en) 2006-06-28 2008-01-03 Riken MUTANT SepRS, AND METHOD FOR SITE-SPECIFIC INTRODUCTION OF PHOSPHOSERINE INTO PROTEIN BY USING THE SAME
US20120077224A1 (en) 2009-06-05 2012-03-29 The Salk Institute For Biological Studies unnatural amino acid incorporation in eukaryotic cells
JP5725467B2 (ja) 2010-08-27 2015-05-27 国立大学法人 東京大学 新規人工翻訳合成系
JP5818237B2 (ja) 2010-09-09 2015-11-18 国立大学法人 東京大学 Ｎ−メチルアミノ酸およびその他の特殊アミノ酸を含む特殊ペプチド化合物ライブラリーの翻訳構築と活性種探索法
EP2647720B1 (en) 2010-12-03 2019-06-19 The University of Tokyo Peptide library production method, peptide library, and screening method
TW202446781A (zh) * 2011-12-28 2024-12-01 日商中外製藥股份有限公司 胜肽化合物之製造方法
JP6754997B2 (ja) 2013-08-26 2020-09-16 国立大学法人 東京大学 大環状ペプチド、その製造方法、及び大環状ペプチドライブラリを用いるスクリーニング方法
WO2015120287A2 (en) 2014-02-06 2015-08-13 Yale University Compositions and methods of use thereof for making polypeptides with many instances of nonstandard amino acids
WO2018052002A1 (ja) 2016-09-13 2018-03-22 第一三共株式会社 Ｔｈｒｏｍｂｏｓｐｏｎｄｉｎ １結合ペプチド
JP7187323B2 (ja) 2017-01-31 2022-12-12 中外製薬株式会社 無細胞翻訳系におけるペプチドの合成方法
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JPWO2019139126A1 (ja) 2018-01-11 2021-04-08 国立大学法人 東京大学 Ｎｔｃｐ阻害剤
EP3904568A4 (en) * 2018-12-26 2024-03-20 Chugai Seiyaku Kabushiki Kaisha Mutated trna for codon expansion
WO2022104001A1 (en) * 2020-11-13 2022-05-19 Bristol-Myers Squibb Company Expanded protein libraries and uses thereof

Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
JP2014217352A (ja) 2013-05-10 2014-11-20 国立大学法人東京大学 ペプチドライブラリの製造方法、ペプチドライブラリ、及びスクリーニング方法
JP2017063672A (ja) 2015-09-29 2017-04-06 国立大学法人 東京大学 タンパク質を製造するための翻訳系、及びタンパク質の製造方法
WO2019077887A1 (ja) 2017-10-16 2019-04-25 国立大学法人東京大学 Ｄ－アミノ酸及びβ－アミノ酸の取り込みを増強するｔＲＮＡのＤ及びＴアームの改変

Non-Patent Citations (2)

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Title
KANDA, T. et al.，Sense Codon-Dependent Introduction of Unnatural Amino Acids into Multiple Sites of a Protein，Biochemical and Biophysical Research Communications，2000年，vol.270，p.1136-1139
TAJIMA, K. et al.，Genetic code expansion via integration of redundant amino acid assignment by finely tuning tRNA pools，Current Opinion in Chemical Biology，2018年，Vol.46，p.212-218

Also Published As

Publication number Publication date
EP4026906A4 (en) 2023-12-27
CN114641569A (zh) 2022-06-17
JPWO2021132546A1 (https=) 2021-07-01
EP4026906A1 (en) 2022-07-13
WO2021132546A1 (ja) 2021-07-01
KR20220121831A (ko) 2022-09-01
JP2025172162A (ja) 2025-11-20
US20230108274A1 (en) 2023-04-06

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