CN114641569A - 翻译用组合物及肽的制备方法 - Google Patents

翻译用组合物及肽的制备方法 Download PDF

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Publication number
CN114641569A
CN114641569A CN202080076538.4A CN202080076538A CN114641569A CN 114641569 A CN114641569 A CN 114641569A CN 202080076538 A CN202080076538 A CN 202080076538A CN 114641569 A CN114641569 A CN 114641569A
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CN
China
Prior art keywords
trna
ile
translation
letter
anticodon
Prior art date
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Pending
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CN202080076538.4A
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English (en)
Chinese (zh)
Inventor
笼谷万奈
山下博子
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Chugai Pharmaceutical Co Ltd
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Chugai Pharmaceutical Co Ltd
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Priority claimed from PCT/JP2019/051241 external-priority patent/WO2020138336A1/ja
Application filed by Chugai Pharmaceutical Co Ltd filed Critical Chugai Pharmaceutical Co Ltd
Publication of CN114641569A publication Critical patent/CN114641569A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
CN202080076538.4A 2019-12-26 2020-12-25 翻译用组合物及肽的制备方法 Pending CN114641569A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JPPCT/JP2019/051241 2019-12-26
PCT/JP2019/051241 WO2020138336A1 (ja) 2018-12-26 2019-12-26 コドン拡張のための変異ｔＲＮＡ
JP2020109292 2020-06-25
JP2020-109292 2020-06-25
PCT/JP2020/048652 WO2021132546A1 (ja) 2019-12-26 2020-12-25 翻訳用組成物及びペプチドの製造方法

Publications (1)

Publication Number Publication Date
CN114641569A true CN114641569A (zh) 2022-06-17

Family

ID=76573318

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202080076538.4A Pending CN114641569A (zh) 2019-12-26 2020-12-25 翻译用组合物及肽的制备方法

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US (1) US20230108274A1 (https=)
EP (1) EP4026906A4 (https=)
JP (2) JP7744244B2 (https=)
KR (1) KR20220121831A (https=)
CN (1) CN114641569A (https=)
WO (1) WO2021132546A1 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW202446781A (zh) 2011-12-28 2024-12-01 日商中外製藥股份有限公司 胜肽化合物之製造方法
EP4166664A1 (en) 2015-03-13 2023-04-19 Chugai Seiyaku Kabushiki Kaisha Modified aminoacyl-trna synthetase and use thereof
JP7187323B2 (ja) 2017-01-31 2022-12-12 中外製薬株式会社 無細胞翻訳系におけるペプチドの合成方法
KR20250007021A (ko) 2017-06-09 2025-01-13 추가이 세이야쿠 가부시키가이샤 N-치환 아미노산을 포함하는 펩타이드의 합성 방법
EP3878836B1 (en) 2018-11-07 2025-07-23 Chugai Seiyaku Kabushiki Kaisha O-substituted serine derivative production method
EP3939962A4 (en) 2019-03-15 2022-12-28 Chugai Seiyaku Kabushiki Kaisha PROCESS FOR PREPARING AN AROMATIC AMINO ACID DERIVATIVE
BR112022007535A2 (pt) 2019-11-07 2022-07-12 Chugai Pharmaceutical Co Ltd Composto peptídico cíclico tendo ação inibitória de kras
JP7823181B2 (ja) 2022-05-06 2026-03-03 中外製薬株式会社 Ｈｒａｓおよびｎｒａｓに対して選択的なｋｒａｓ阻害作用を有する環状化合物

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1694958A (zh) * 2002-09-13 2005-11-09 昆士兰大学 以密码子翻译效率为基础的基因表达系统
CN103189522A (zh) * 2010-08-27 2013-07-03 国立大学法人东京大学 新型人工翻译合成系统
US20160272964A1 (en) * 2013-05-10 2016-09-22 The University Of Tokyo Method for Producing Peptide Library, Peptide Library, and Screening Method
US20180298390A1 (en) * 2015-09-29 2018-10-18 Hiroshi Murakami Novel Genetic Code and Translation System for Producing Polypeptide, and Method for Producing Polypeptide

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006135096A1 (ja) 2005-06-14 2006-12-21 Gifu University 部位特異的にタンパク質にチロシンアナログを導入する方法
WO2007061136A1 (ja) 2005-11-24 2007-05-31 Riken 非天然型アミノ酸を組み込んだタンパク質の製造方法
WO2007066627A1 (ja) 2005-12-06 2007-06-14 The University Of Tokyo 多目的アシル化触媒とその用途
AU2007224019A1 (en) 2006-03-03 2007-09-13 California Institute Of Technology Site-specific incorporation of amino acids into molecules
WO2008001947A1 (en) 2006-06-28 2008-01-03 Riken MUTANT SepRS, AND METHOD FOR SITE-SPECIFIC INTRODUCTION OF PHOSPHOSERINE INTO PROTEIN BY USING THE SAME
US20120077224A1 (en) 2009-06-05 2012-03-29 The Salk Institute For Biological Studies unnatural amino acid incorporation in eukaryotic cells
JP5818237B2 (ja) 2010-09-09 2015-11-18 国立大学法人 東京大学 Ｎ−メチルアミノ酸およびその他の特殊アミノ酸を含む特殊ペプチド化合物ライブラリーの翻訳構築と活性種探索法
EP2647720B1 (en) 2010-12-03 2019-06-19 The University of Tokyo Peptide library production method, peptide library, and screening method
TW202446781A (zh) * 2011-12-28 2024-12-01 日商中外製藥股份有限公司 胜肽化合物之製造方法
JP6754997B2 (ja) 2013-08-26 2020-09-16 国立大学法人 東京大学 大環状ペプチド、その製造方法、及び大環状ペプチドライブラリを用いるスクリーニング方法
WO2015120287A2 (en) 2014-02-06 2015-08-13 Yale University Compositions and methods of use thereof for making polypeptides with many instances of nonstandard amino acids
WO2018052002A1 (ja) 2016-09-13 2018-03-22 第一三共株式会社 Ｔｈｒｏｍｂｏｓｐｏｎｄｉｎ １結合ペプチド
JP7187323B2 (ja) 2017-01-31 2022-12-12 中外製薬株式会社 無細胞翻訳系におけるペプチドの合成方法
CN110869544B (zh) 2017-06-09 2024-03-08 中外制药株式会社 膜透过性高的环状肽化合物及包含其的文库
EP3699276A4 (en) * 2017-10-16 2021-07-28 The University Of Tokyo MODIFICATION OF ARM D AND T ARNT IMPROVING DAMINO ACID AND BETA AMINO ACID CAPTURE
JPWO2019139126A1 (ja) 2018-01-11 2021-04-08 国立大学法人 東京大学 Ｎｔｃｐ阻害剤
EP3904568A4 (en) * 2018-12-26 2024-03-20 Chugai Seiyaku Kabushiki Kaisha Mutated trna for codon expansion
WO2022104001A1 (en) * 2020-11-13 2022-05-19 Bristol-Myers Squibb Company Expanded protein libraries and uses thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1694958A (zh) * 2002-09-13 2005-11-09 昆士兰大学 以密码子翻译效率为基础的基因表达系统
CN103189522A (zh) * 2010-08-27 2013-07-03 国立大学法人东京大学 新型人工翻译合成系统
US20160272964A1 (en) * 2013-05-10 2016-09-22 The University Of Tokyo Method for Producing Peptide Library, Peptide Library, and Screening Method
US20180298390A1 (en) * 2015-09-29 2018-10-18 Hiroshi Murakami Novel Genetic Code and Translation System for Producing Polypeptide, and Method for Producing Polypeptide

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
TAKAYUKI KANDA ET AL.: ""Sense Codon-Dependent Introduction of Unnatural Amino Acids into Multiple Sites of a Protein"", 《BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS》, vol. 270, pages 1137 - 1139 *
王志鹏等: ""非天然氨基酸引入法制备含有 新型赖氨酸翻译后修饰蛋白质"", 《化学教育（中英文）》, vol. 39, no. 18, pages 9 - 13 *

Also Published As

Publication number Publication date
EP4026906A4 (en) 2023-12-27
JPWO2021132546A1 (https=) 2021-07-01
EP4026906A1 (en) 2022-07-13
WO2021132546A1 (ja) 2021-07-01
JP7744244B2 (ja) 2025-09-25
KR20220121831A (ko) 2022-09-01
JP2025172162A (ja) 2025-11-20
US20230108274A1 (en) 2023-04-06

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