JP7661229B2 - ステロイドの１，２－脱水素のための微生物の生物工学的最適化 - Google Patents

ステロイドの１，２－脱水素のための微生物の生物工学的最適化 Download PDF

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JP7661229B2
JP7661229B2 JP2021546256A JP2021546256A JP7661229B2 JP 7661229 B2 JP7661229 B2 JP 7661229B2 JP 2021546256 A JP2021546256 A JP 2021546256A JP 2021546256 A JP2021546256 A JP 2021546256A JP 7661229 B2 JP7661229 B2 JP 7661229B2
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hsdh
dehydrogenated
bacterium
steroid
dehydrogenase activity
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クラウス・トビアス・ラッテマン
ベルント・ヤノハ
ハンス－ファルク・ラッサー
セバスティアン・リーソム
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ユーロ・エー・ピー・アイ・フランス
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07J—STEROIDS
    • C07J5/00—Normal steroids containing carbon, hydrogen, halogen or oxygen, substituted in position 17 beta by a chain of two carbon atoms, e.g. pregnane and substituted in position 21 by only one singly bound oxygen atom, i.e. only one oxygen bound to position 21 by a single bond
    • C07J5/0046—Normal steroids containing carbon, hydrogen, halogen or oxygen, substituted in position 17 beta by a chain of two carbon atoms, e.g. pregnane and substituted in position 21 by only one singly bound oxygen atom, i.e. only one oxygen bound to position 21 by a single bond substituted in position 17 alfa
    • C07J5/0053—Normal steroids containing carbon, hydrogen, halogen or oxygen, substituted in position 17 beta by a chain of two carbon atoms, e.g. pregnane and substituted in position 21 by only one singly bound oxygen atom, i.e. only one oxygen bound to position 21 by a single bond substituted in position 17 alfa not substituted in position 16
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/02—Dehydrogenating; Dehydroxylating
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/365—Nocardia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01053—3-Alpha (or 20-beta)-hydroxysteroid dehydrogenase (1.1.1.53)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/99—Oxidoreductases acting on the CH-CH group of donors (1.3) with other acceptors (1.3.99)
    • C12Y103/99004—3-Oxosteroid 1-dehydrogenase (1.3.99.4)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Steroid Compounds (AREA)
JP2021546256A 2019-02-08 2020-02-07 ステロイドの１，２－脱水素のための微生物の生物工学的最適化 Active JP7661229B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2024218426A JP2025038080A (ja) 2019-02-08 2024-12-13 ステロイドの１，２－脱水素のための微生物の生物工学的最適化

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP19305153 2019-02-08
EP19305153.9 2019-02-08
PCT/EP2020/053167 WO2020161317A1 (en) 2019-02-08 2020-02-07 Biotechnological optimization of microorganisms for the 1,2-dehydrogenation of steroids

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JP7661229B2 true JP7661229B2 (ja) 2025-04-14

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JP2024218426A Withdrawn JP2025038080A (ja) 2019-02-08 2024-12-13 ステロイドの１，２－脱水素のための微生物の生物工学的最適化

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US (1) US20220136029A1 (https=)
EP (1) EP3921414A1 (https=)
JP (2) JP7661229B2 (https=)
KR (1) KR20210125490A (https=)
CN (1) CN113423827A (https=)
AU (1) AU2020219424A1 (https=)
BR (1) BR112021015481A2 (https=)
CA (1) CA3128954A1 (https=)
IL (1) IL285386A (https=)
MX (1) MX2021009515A (https=)
SG (1) SG11202108529WA (https=)
TW (1) TWI852986B (https=)
WO (1) WO2020161317A1 (https=)
ZA (1) ZA202105566B (https=)

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Publication number Priority date Publication date Assignee Title
SG11202108529WA (en) * 2019-02-08 2021-09-29 Sanofi Sa Biotechnological optimization of microorganisms for the 1,2-dehydrogenation of steroids
CN118325997B (zh) * 2024-04-10 2025-07-22 湖南玉新药业有限公司 采用诺卡氏菌一步发酵生产泼尼松龙的方法
CN119464157B (zh) * 2025-01-13 2025-06-27 山东绿邦生物科技有限公司 居瘤胃棒状杆菌、微生物菌剂及其应用

Citations (1)

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Publication number Priority date Publication date Assignee Title
WO2001031050A1 (en) 1999-10-22 2001-05-03 Akzo Nobel N.V. MICROBIAL 9α-HYDROXYLATION OF STEROIDS

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JP2007515490A (ja) * 2003-12-22 2007-06-14 アムジェン インコーポレーティッド アリールスルホンアミド化合物およびそれに関連する使用法
US20050239853A1 (en) * 2004-02-04 2005-10-27 Tjeerd Barf New compounds
WO2006124141A2 (en) * 2005-04-05 2006-11-23 Taro Pharmaceuticals North America, Inc. A process of preparing prednisolone devoid of steroid-derived impurities using recombinant e. coli transformed with a δ1-dehydrogenase gene
AU2013200516B2 (en) * 2006-07-12 2015-09-03 University Of Tennessee Research Foundation Substituted Acylanilides and Methods of Use Thereof
DK2084519T3 (da) * 2006-10-10 2012-08-20 Los Alamos Nat Security Llc Røntgenfluorescens-analysefremgangsmåde
CN106754775B (zh) * 2016-12-23 2019-08-20 华东理工大学 一种羰基还原酶突变体及其基因与应用
WO2018204764A1 (en) * 2017-05-05 2018-11-08 Camp4 Therapeutics Corporation Identification and targeted modulation of gene signaling networks
AU2019384074B2 (en) * 2018-11-20 2025-04-10 Sparrow Pharmaceuticals, Inc. Methods for administering corticosteroids
SG11202108529WA (en) * 2019-02-08 2021-09-29 Sanofi Sa Biotechnological optimization of microorganisms for the 1,2-dehydrogenation of steroids

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001031050A1 (en) 1999-10-22 2001-05-03 Akzo Nobel N.V. MICROBIAL 9α-HYDROXYLATION OF STEROIDS

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Appl. Microbiol. Biotechnol.，2013年，Vol.97，pp.9503-9514
Enzyme Microb. Technol.，1989年，Vol.11，pp.116-120

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JP2025038080A (ja) 2025-03-18
US20220136029A1 (en) 2022-05-05
TW202045720A (zh) 2020-12-16
KR20210125490A (ko) 2021-10-18
ZA202105566B (en) 2024-05-30
JP2022519711A (ja) 2022-03-24
AU2020219424A1 (en) 2021-08-26
TWI852986B (zh) 2024-08-21
SG11202108529WA (en) 2021-09-29
MX2021009515A (es) 2021-10-26
CA3128954A1 (en) 2020-08-13
IL285386A (en) 2021-09-30
CN113423827A (zh) 2021-09-21
BR112021015481A2 (pt) 2021-12-07
EP3921414A1 (en) 2021-12-15
WO2020161317A1 (en) 2020-08-13

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