AU2020219424A1 - Biotechnological optimization of microorganisms for the 1,2-dehydrogenation of steroids - Google Patents

Biotechnological optimization of microorganisms for the 1,2-dehydrogenation of steroids Download PDF

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Publication number
AU2020219424A1
AU2020219424A1 AU2020219424A AU2020219424A AU2020219424A1 AU 2020219424 A1 AU2020219424 A1 AU 2020219424A1 AU 2020219424 A AU2020219424 A AU 2020219424A AU 2020219424 A AU2020219424 A AU 2020219424A AU 2020219424 A1 AU2020219424 A1 AU 2020219424A1
Authority
AU
Australia
Prior art keywords
bacterium
steroid
dehydrogenated
dehydrogenase activity
genetically modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
AU2020219424A
Other languages
English (en)
Inventor
Bernd Janocha
Claus Tobias Lattemann
Hans-Falk Rasser
Sebastian Rissom
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Euroapi France SAS
Original Assignee
Euroapi France SAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Euroapi France SAS filed Critical Euroapi France SAS
Publication of AU2020219424A1 publication Critical patent/AU2020219424A1/en
Assigned to EUROAPI FRANCE reassignment EUROAPI FRANCE Request for Assignment Assignors: SANOFI
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/02—Dehydrogenating; Dehydroxylating
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07J—STEROIDS
    • C07J5/00—Normal steroids containing carbon, hydrogen, halogen or oxygen, substituted in position 17 beta by a chain of two carbon atoms, e.g. pregnane and substituted in position 21 by only one singly bound oxygen atom, i.e. only one oxygen bound to position 21 by a single bond
    • C07J5/0046—Normal steroids containing carbon, hydrogen, halogen or oxygen, substituted in position 17 beta by a chain of two carbon atoms, e.g. pregnane and substituted in position 21 by only one singly bound oxygen atom, i.e. only one oxygen bound to position 21 by a single bond substituted in position 17 alfa
    • C07J5/0053—Normal steroids containing carbon, hydrogen, halogen or oxygen, substituted in position 17 beta by a chain of two carbon atoms, e.g. pregnane and substituted in position 21 by only one singly bound oxygen atom, i.e. only one oxygen bound to position 21 by a single bond substituted in position 17 alfa not substituted in position 16
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/365—Nocardia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01053—3-Alpha (or 20-beta)-hydroxysteroid dehydrogenase (1.1.1.53)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/99—Oxidoreductases acting on the CH-CH group of donors (1.3) with other acceptors (1.3.99)
    • C12Y103/99004—3-Oxosteroid 1-dehydrogenase (1.3.99.4)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Steroid Compounds (AREA)
AU2020219424A 2019-02-08 2020-02-07 Biotechnological optimization of microorganisms for the 1,2-dehydrogenation of steroids Pending AU2020219424A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP19305153 2019-02-08
EP19305153.9 2019-02-08
PCT/EP2020/053167 WO2020161317A1 (en) 2019-02-08 2020-02-07 Biotechnological optimization of microorganisms for the 1,2-dehydrogenation of steroids

Publications (1)

Publication Number Publication Date
AU2020219424A1 true AU2020219424A1 (en) 2021-08-26

Family

ID=66554300

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2020219424A Pending AU2020219424A1 (en) 2019-02-08 2020-02-07 Biotechnological optimization of microorganisms for the 1,2-dehydrogenation of steroids

Country Status (14)

Country Link
US (1) US20220136029A1 (https=)
EP (1) EP3921414A1 (https=)
JP (2) JP7661229B2 (https=)
KR (1) KR20210125490A (https=)
CN (1) CN113423827A (https=)
AU (1) AU2020219424A1 (https=)
BR (1) BR112021015481A2 (https=)
CA (1) CA3128954A1 (https=)
IL (1) IL285386A (https=)
MX (1) MX2021009515A (https=)
SG (1) SG11202108529WA (https=)
TW (1) TWI852986B (https=)
WO (1) WO2020161317A1 (https=)
ZA (1) ZA202105566B (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11202108529WA (en) * 2019-02-08 2021-09-29 Sanofi Sa Biotechnological optimization of microorganisms for the 1,2-dehydrogenation of steroids
CN118325997B (zh) * 2024-04-10 2025-07-22 湖南玉新药业有限公司 采用诺卡氏菌一步发酵生产泼尼松龙的方法
CN119464157B (zh) * 2025-01-13 2025-06-27 山东绿邦生物科技有限公司 居瘤胃棒状杆菌、微生物菌剂及其应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1232278A1 (en) * 1999-10-22 2002-08-21 Akzo Nobel N.V. MICROBIAL 9$g(a)-HYDROXYLATION OF STEROIDS
JP2007515490A (ja) * 2003-12-22 2007-06-14 アムジェン インコーポレーティッド アリールスルホンアミド化合物およびそれに関連する使用法
US20050239853A1 (en) * 2004-02-04 2005-10-27 Tjeerd Barf New compounds
WO2006124141A2 (en) * 2005-04-05 2006-11-23 Taro Pharmaceuticals North America, Inc. A process of preparing prednisolone devoid of steroid-derived impurities using recombinant e. coli transformed with a δ1-dehydrogenase gene
AU2013200516B2 (en) * 2006-07-12 2015-09-03 University Of Tennessee Research Foundation Substituted Acylanilides and Methods of Use Thereof
DK2084519T3 (da) * 2006-10-10 2012-08-20 Los Alamos Nat Security Llc Røntgenfluorescens-analysefremgangsmåde
CN106754775B (zh) * 2016-12-23 2019-08-20 华东理工大学 一种羰基还原酶突变体及其基因与应用
WO2018204764A1 (en) * 2017-05-05 2018-11-08 Camp4 Therapeutics Corporation Identification and targeted modulation of gene signaling networks
AU2019384074B2 (en) * 2018-11-20 2025-04-10 Sparrow Pharmaceuticals, Inc. Methods for administering corticosteroids
SG11202108529WA (en) * 2019-02-08 2021-09-29 Sanofi Sa Biotechnological optimization of microorganisms for the 1,2-dehydrogenation of steroids

Also Published As

Publication number Publication date
JP2025038080A (ja) 2025-03-18
US20220136029A1 (en) 2022-05-05
TW202045720A (zh) 2020-12-16
KR20210125490A (ko) 2021-10-18
ZA202105566B (en) 2024-05-30
JP2022519711A (ja) 2022-03-24
TWI852986B (zh) 2024-08-21
SG11202108529WA (en) 2021-09-29
MX2021009515A (es) 2021-10-26
CA3128954A1 (en) 2020-08-13
IL285386A (en) 2021-09-30
CN113423827A (zh) 2021-09-21
BR112021015481A2 (pt) 2021-12-07
EP3921414A1 (en) 2021-12-15
WO2020161317A1 (en) 2020-08-13
JP7661229B2 (ja) 2025-04-14

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