CN113423827A - 用于类固醇的1,2-脱氢的微生物的生物技术优化 - Google Patents

用于类固醇的1,2-脱氢的微生物的生物技术优化 Download PDF

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Publication number
CN113423827A
CN113423827A CN202080013236.2A CN202080013236A CN113423827A CN 113423827 A CN113423827 A CN 113423827A CN 202080013236 A CN202080013236 A CN 202080013236A CN 113423827 A CN113423827 A CN 113423827A
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China
Prior art keywords
bacterium
dehydrogenase activity
hsdh
genetically modified
steroid
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Pending
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CN202080013236.2A
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English (en)
Chinese (zh)
Inventor
C·T·拉特曼
B·雅诺哈
H-F·莱泽
S·里佐姆
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Yuruoyapi Co
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Sanofi SA
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/02—Dehydrogenating; Dehydroxylating
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07J—STEROIDS
    • C07J5/00—Normal steroids containing carbon, hydrogen, halogen or oxygen, substituted in position 17 beta by a chain of two carbon atoms, e.g. pregnane and substituted in position 21 by only one singly bound oxygen atom, i.e. only one oxygen bound to position 21 by a single bond
    • C07J5/0046—Normal steroids containing carbon, hydrogen, halogen or oxygen, substituted in position 17 beta by a chain of two carbon atoms, e.g. pregnane and substituted in position 21 by only one singly bound oxygen atom, i.e. only one oxygen bound to position 21 by a single bond substituted in position 17 alfa
    • C07J5/0053—Normal steroids containing carbon, hydrogen, halogen or oxygen, substituted in position 17 beta by a chain of two carbon atoms, e.g. pregnane and substituted in position 21 by only one singly bound oxygen atom, i.e. only one oxygen bound to position 21 by a single bond substituted in position 17 alfa not substituted in position 16
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/365—Nocardia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01053—3-Alpha (or 20-beta)-hydroxysteroid dehydrogenase (1.1.1.53)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/99—Oxidoreductases acting on the CH-CH group of donors (1.3) with other acceptors (1.3.99)
    • C12Y103/99004—3-Oxosteroid 1-dehydrogenase (1.3.99.4)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Steroid Compounds (AREA)
CN202080013236.2A 2019-02-08 2020-02-07 用于类固醇的1,2-脱氢的微生物的生物技术优化 Pending CN113423827A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP19305153 2019-02-08
EP19305153.9 2019-02-08
PCT/EP2020/053167 WO2020161317A1 (en) 2019-02-08 2020-02-07 Biotechnological optimization of microorganisms for the 1,2-dehydrogenation of steroids

Publications (1)

Publication Number Publication Date
CN113423827A true CN113423827A (zh) 2021-09-21

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Family Applications (1)

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CN202080013236.2A Pending CN113423827A (zh) 2019-02-08 2020-02-07 用于类固醇的1,2-脱氢的微生物的生物技术优化

Country Status (14)

Country Link
US (1) US20220136029A1 (https=)
EP (1) EP3921414A1 (https=)
JP (2) JP7661229B2 (https=)
KR (1) KR20210125490A (https=)
CN (1) CN113423827A (https=)
AU (1) AU2020219424A1 (https=)
BR (1) BR112021015481A2 (https=)
CA (1) CA3128954A1 (https=)
IL (1) IL285386A (https=)
MX (1) MX2021009515A (https=)
SG (1) SG11202108529WA (https=)
TW (1) TWI852986B (https=)
WO (1) WO2020161317A1 (https=)
ZA (1) ZA202105566B (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN118325997A (zh) * 2024-04-10 2024-07-12 湖南玉新药业有限公司 采用诺卡氏菌一步发酵生产泼尼松龙的方法
CN119464157A (zh) * 2025-01-13 2025-02-18 山东绿邦生物科技有限公司 居瘤胃棒状杆菌、微生物菌剂及其应用

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11202108529WA (en) * 2019-02-08 2021-09-29 Sanofi Sa Biotechnological optimization of microorganisms for the 1,2-dehydrogenation of steroids

Citations (9)

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TW200530206A (en) * 2004-02-04 2005-09-16 Biovitrum Ab New compounds
TW200533335A (en) * 2003-12-22 2005-10-16 Amgen Sf Llc Aryl sulfonamide compounds and uses related thereto
WO2006124141A2 (en) * 2005-04-05 2006-11-23 Taro Pharmaceuticals North America, Inc. A process of preparing prednisolone devoid of steroid-derived impurities using recombinant e. coli transformed with a δ1-dehydrogenase gene
EP2511844A2 (en) * 2006-10-10 2012-10-17 Los Alamos National Security, LLC Advanced drug development and manufacturing
AU2013200516A1 (en) * 2006-07-12 2013-02-21 University Of Tennessee Research Foundation Substituted Acylanilides and Methods of Use Thereof
CN106754775A (zh) * 2016-12-23 2017-05-31 华东理工大学 一种羰基还原酶突变体及其基因与应用
TW202045720A (zh) * 2019-02-08 2020-12-16 法商賽諾菲公司 微生物之生物技術最佳化
US20210254056A1 (en) * 2017-05-05 2021-08-19 Camp4 Therapeutics Corporation Identification and targeted modulation of gene signaling networks
CN113329754A (zh) * 2018-11-20 2021-08-31 麻雀制药股份有限公司 用于施用皮质类固醇的方法

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EP1232278A1 (en) * 1999-10-22 2002-08-21 Akzo Nobel N.V. MICROBIAL 9$g(a)-HYDROXYLATION OF STEROIDS

Patent Citations (11)

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TW200533335A (en) * 2003-12-22 2005-10-16 Amgen Sf Llc Aryl sulfonamide compounds and uses related thereto
TW200530206A (en) * 2004-02-04 2005-09-16 Biovitrum Ab New compounds
WO2006124141A2 (en) * 2005-04-05 2006-11-23 Taro Pharmaceuticals North America, Inc. A process of preparing prednisolone devoid of steroid-derived impurities using recombinant e. coli transformed with a δ1-dehydrogenase gene
US20070015236A1 (en) * 2005-04-05 2007-01-18 Albert Fliss Process of preparing prednisolone devoid of steroid-derived impurities using recombinant E. coli transformed with A delta¹-dehydrogenase gene
AU2013200516A1 (en) * 2006-07-12 2013-02-21 University Of Tennessee Research Foundation Substituted Acylanilides and Methods of Use Thereof
EP2511844A2 (en) * 2006-10-10 2012-10-17 Los Alamos National Security, LLC Advanced drug development and manufacturing
CN106754775A (zh) * 2016-12-23 2017-05-31 华东理工大学 一种羰基还原酶突变体及其基因与应用
US20210254056A1 (en) * 2017-05-05 2021-08-19 Camp4 Therapeutics Corporation Identification and targeted modulation of gene signaling networks
CN113329754A (zh) * 2018-11-20 2021-08-31 麻雀制药股份有限公司 用于施用皮质类固醇的方法
TW202045720A (zh) * 2019-02-08 2020-12-16 法商賽諾菲公司 微生物之生物技術最佳化
US20220136029A1 (en) * 2019-02-08 2022-05-05 Sanofi Biotechnological optimization of microorganisms for the 1,2-dehydrogenation of steroids

Non-Patent Citations (3)

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DEBASHIS GHOSH 等: "Three domentional structure of holo 3-alpha, 20-beta-hydroxysteroid dehydrogenase:a member of a short-chian dehydrogenase family", 《PRI.NATI.ACAD.SCI.USA》, 1 November 1991 (1991-11-01) *
DOUGLAS F. COVEY 等: "Inactivation of Streptomyces hydrogenans 20P-Hydroxysteroid Dehydrogenase by an Enzyme-Generated Ethoxyacetylenic Ketone in the Presence of a Thiol Scavenger", 《BIOCHEMISTRY》, vol. 25, no. 23, 18 November 1986 (1986-11-18), XP055599772, DOI: 10.1021/bi00371a007 *
李京宝;蔺潇;周虹;: "糖皮质激素与骨", 中华骨质疏松和骨矿盐疾病杂志, no. 04, 20 December 2013 (2013-12-20) *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN118325997A (zh) * 2024-04-10 2024-07-12 湖南玉新药业有限公司 采用诺卡氏菌一步发酵生产泼尼松龙的方法
CN119464157A (zh) * 2025-01-13 2025-02-18 山东绿邦生物科技有限公司 居瘤胃棒状杆菌、微生物菌剂及其应用
CN119464157B (zh) * 2025-01-13 2025-06-27 山东绿邦生物科技有限公司 居瘤胃棒状杆菌、微生物菌剂及其应用

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JP2025038080A (ja) 2025-03-18
US20220136029A1 (en) 2022-05-05
TW202045720A (zh) 2020-12-16
KR20210125490A (ko) 2021-10-18
ZA202105566B (en) 2024-05-30
JP2022519711A (ja) 2022-03-24
AU2020219424A1 (en) 2021-08-26
TWI852986B (zh) 2024-08-21
SG11202108529WA (en) 2021-09-29
MX2021009515A (es) 2021-10-26
CA3128954A1 (en) 2020-08-13
IL285386A (en) 2021-09-30
BR112021015481A2 (pt) 2021-12-07
EP3921414A1 (en) 2021-12-15
WO2020161317A1 (en) 2020-08-13
JP7661229B2 (ja) 2025-04-14

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