BR112021015481A2 - Otimização biotecnológica de micro-organismos para a 1,2-desidrogenação de esteroides - Google Patents

Otimização biotecnológica de micro-organismos para a 1,2-desidrogenação de esteroides

Info

Publication number
BR112021015481A2
BR112021015481A2 BR112021015481A BR112021015481A BR112021015481A2 BR 112021015481 A2 BR112021015481 A2 BR 112021015481A2 BR 112021015481 A BR112021015481 A BR 112021015481A BR 112021015481 A BR112021015481 A BR 112021015481A BR 112021015481 A2 BR112021015481 A2 BR 112021015481A2
Authority
BR
Brazil
Prior art keywords
dehydrogenation
steroids
biotechnological
microorganisms
optimization
Prior art date
Application number
BR112021015481A
Other languages
English (en)
Inventor
Bernd Janocha
Tobias LATTEMANN Claus
Hans-Falk Rasser
Sebastian Rissom
Original Assignee
Sanofi Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Sa filed Critical Sanofi Sa
Publication of BR112021015481A2 publication Critical patent/BR112021015481A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/02—Dehydrogenating; Dehydroxylating
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01053—3-Alpha (or 20-beta)-hydroxysteroid dehydrogenase (1.1.1.53)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/99—Oxidoreductases acting on the CH-CH group of donors (1.3) with other acceptors (1.3.99)
    • C12Y103/99004—3-Oxosteroid 1-dehydrogenase (1.3.99.4)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07J—STEROIDS
    • C07J5/00—Normal steroids containing carbon, hydrogen, halogen or oxygen, substituted in position 17 beta by a chain of two carbon atoms, e.g. pregnane and substituted in position 21 by only one singly bound oxygen atom, i.e. only one oxygen bound to position 21 by a single bond
    • C07J5/0046—Normal steroids containing carbon, hydrogen, halogen or oxygen, substituted in position 17 beta by a chain of two carbon atoms, e.g. pregnane and substituted in position 21 by only one singly bound oxygen atom, i.e. only one oxygen bound to position 21 by a single bond substituted in position 17 alfa
    • C07J5/0053—Normal steroids containing carbon, hydrogen, halogen or oxygen, substituted in position 17 beta by a chain of two carbon atoms, e.g. pregnane and substituted in position 21 by only one singly bound oxygen atom, i.e. only one oxygen bound to position 21 by a single bond substituted in position 17 alfa not substituted in position 16
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/365—Nocardia

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Steroid Compounds (AREA)

Abstract

otimização biotecnológica de micro-organismos para a 1,2-desidrogenação de esteroides. a presente invenção refere-se a uma bactéria geneticamente modificada e à sua aplicação industrial, em particular na 1,2-desidrogenação de esteroides.
BR112021015481A 2019-02-08 2020-02-07 Otimização biotecnológica de micro-organismos para a 1,2-desidrogenação de esteroides BR112021015481A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP19305153 2019-02-08
PCT/EP2020/053167 WO2020161317A1 (en) 2019-02-08 2020-02-07 Biotechnological optimization of microorganisms for the 1,2-dehydrogenation of steroids

Publications (1)

Publication Number Publication Date
BR112021015481A2 true BR112021015481A2 (pt) 2021-12-07

Family

ID=66554300

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021015481A BR112021015481A2 (pt) 2019-02-08 2020-02-07 Otimização biotecnológica de micro-organismos para a 1,2-desidrogenação de esteroides

Country Status (14)

Country Link
US (1) US20220136029A1 (pt)
EP (1) EP3921414A1 (pt)
JP (1) JP2022519711A (pt)
KR (1) KR20210125490A (pt)
CN (1) CN113423827A (pt)
AU (1) AU2020219424A1 (pt)
BR (1) BR112021015481A2 (pt)
CA (1) CA3128954A1 (pt)
IL (1) IL285386A (pt)
MX (1) MX2021009515A (pt)
SG (1) SG11202108529WA (pt)
TW (1) TW202045720A (pt)
WO (1) WO2020161317A1 (pt)
ZA (1) ZA202105566B (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112021015481A2 (pt) * 2019-02-08 2021-12-07 Sanofi Sa Otimização biotecnológica de micro-organismos para a 1,2-desidrogenação de esteroides

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005063247A1 (en) * 2003-12-22 2005-07-14 Amgen Inc. Aryl sulfonamide compounds and uses related thereto
US20050239853A1 (en) * 2004-02-04 2005-10-27 Tjeerd Barf New compounds
US20070015236A1 (en) * 2005-04-05 2007-01-18 Albert Fliss Process of preparing prednisolone devoid of steroid-derived impurities using recombinant E. coli transformed with A delta¹-dehydrogenase gene
AU2013200516B2 (en) * 2006-07-12 2015-09-03 University Of Tennessee Research Foundation Substituted Acylanilides and Methods of Use Thereof
EP2084519B1 (en) * 2006-10-10 2012-08-01 Los Alamos National Security, LLC X-ray fluorescence analysis method
CN106754775B (zh) * 2016-12-23 2019-08-20 华东理工大学 一种羰基还原酶突变体及其基因与应用
WO2018204764A1 (en) * 2017-05-05 2018-11-08 Camp4 Therapeutics Corporation Identification and targeted modulation of gene signaling networks
JP7472122B2 (ja) * 2018-11-20 2024-04-22 スパロー ファーマシューティカルズ，インコーポレーテッド コルチコステロイドを投与する方法
BR112021015481A2 (pt) * 2019-02-08 2021-12-07 Sanofi Sa Otimização biotecnológica de micro-organismos para a 1,2-desidrogenação de esteroides

Also Published As

Publication number Publication date
ZA202105566B (en) 2024-05-30
TW202045720A (zh) 2020-12-16
CN113423827A (zh) 2021-09-21
IL285386A (en) 2021-09-30
JP2022519711A (ja) 2022-03-24
CA3128954A1 (en) 2020-08-13
MX2021009515A (es) 2021-10-26
WO2020161317A1 (en) 2020-08-13
KR20210125490A (ko) 2021-10-18
SG11202108529WA (en) 2021-09-29
EP3921414A1 (en) 2021-12-15
US20220136029A1 (en) 2022-05-05
AU2020219424A1 (en) 2021-08-26

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