JP7402864B2 - ポリユビキチンリンカーに結合された線形多機能性マルチマー生体分子及びその用途 - Google Patents

ポリユビキチンリンカーに結合された線形多機能性マルチマー生体分子及びその用途 Download PDF

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JP7402864B2
JP7402864B2 JP2021517169A JP2021517169A JP7402864B2 JP 7402864 B2 JP7402864 B2 JP 7402864B2 JP 2021517169 A JP2021517169 A JP 2021517169A JP 2021517169 A JP2021517169 A JP 2021517169A JP 7402864 B2 JP7402864 B2 JP 7402864B2
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JP2021525807A (ja
JPWO2019231208A5 (https=
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ソンジン パク
デ ソン イム
ジェヨン チョ
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ワンジーン バイオテクノロジー インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
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    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/104—Aminoacyltransferases (2.3.2)
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
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    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01009—D-Xylulose reductase (1.1.1.9), i.e. xylitol dehydrogenase
    • C—CHEMISTRY; METALLURGY
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    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01307—D-Xylose reductase (1.1.1.307)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/03—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with oxygen as acceptor (1.2.3)
    • C12Y102/03003—Pyruvate oxidase (1.2.3.3)
    • C—CHEMISTRY; METALLURGY
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    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/02—Aminoacyltransferases (2.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • C12Y301/02015—Ubiquitin thiolesterase (3.1.2.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01003—Oxaloacetate decarboxylase (4.1.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/02—Acid—amino-acid ligases (peptide synthases)(6.3.2)
    • C12Y603/02021—Ubiquitin--calmodulin ligase (6.3.2.21)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)
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    • C12Y—ENZYMES
    • C12Y602/00—Ligases forming carbon-sulfur bonds (6.2)
    • C12Y602/01—Acid-Thiol Ligases (6.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/02—Acid—amino-acid ligases (peptide synthases)(6.3.2)
    • C12Y603/02019—Ubiquitin-protein ligase (6.3.2.19), i.e. ubiquitin-conjugating enzyme

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  • Immunology (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Gastroenterology & Hepatology (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2021517169A 2018-05-28 2019-05-28 ポリユビキチンリンカーに結合された線形多機能性マルチマー生体分子及びその用途 Active JP7402864B2 (ja)

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KR20180060502 2018-05-28
KR10-2018-0060502 2018-05-28
PCT/KR2019/006376 WO2019231208A1 (ko) 2018-05-28 2019-05-28 폴리유비퀴틴 링커에 결합된 선형 다기능성 멀티머 생체분자 및 이의 용도

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JP2021525807A JP2021525807A (ja) 2021-09-27
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JP7402864B2 true JP7402864B2 (ja) 2023-12-21

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EP (1) EP3805256A4 (https=)
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KR (2) KR20190135393A (https=)
CN (1) CN112204046A (https=)
AU (2) AU2019276622A1 (https=)
CA (1) CA3101185A1 (https=)
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BR112022010445A2 (pt) * 2019-11-27 2022-09-06 Onegene Biotechnology Inc Polímero biomolecular multifuncional, multiespecífico, multimérico , método para preparar um polímero biomolecular multifuncional, multiespecífico, multimérico
EP4144375A4 (en) * 2020-04-29 2024-11-06 Onegene Biotechnology Inc. NOVEL PROTEIN CONJUGATE AND ITS USE FOR PREVENTION OR TREATMENT OF NONALCOHOLIC STEATOHEPATITIS, OBESITY AND DIABETES
CN115678985B (zh) * 2022-10-26 2025-07-29 华南农业大学 March5在鉴别猪对蓝耳病毒易感性及制备药物中的应用
CN119876239A (zh) * 2025-01-27 2025-04-25 吉林大学 拟南芥AtUPL5基因在提高植物抗性方面的应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014090721A (ja) 2012-10-31 2014-05-19 Samsung Electronics Co Ltd 二重特異性（ｂｉｓｐｅｃｉｆｉｃ）抗原結合蛋白質複合体及び二重特異性抗体の製造方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7354722B1 (en) * 1999-06-11 2008-04-08 Signal Pharmaceuticals Modulators of Smurf and BMP/TGFβ signaling pathways
ATE490968T1 (de) * 2001-05-04 2010-12-15 Cornell Res Foundation Inc Schnell spaltbares sumofusionsprotein- expressionssystem für schwer zu exprimierende proteine
DE10324447A1 (de) * 2003-05-28 2004-12-30 Scil Proteins Gmbh Generierung künstlicher Bindungsproteine auf der Grundlage von Ubiquitin
AU2004254600A1 (en) * 2003-06-26 2005-01-13 Lifesensors, Inc. Methods and compositions for enhanced protein expression and purification
KR20050050824A (ko) 2003-11-26 2005-06-01 세메스 주식회사 베이크 장치에서의 웨이퍼 안착 상태를 감지하여 인터락을제어하기 위한 시스템 및 그의 제어 방법
DE102004049479A1 (de) * 2004-10-11 2006-04-13 Scil Proteins Gmbh Proteinkonjugate zur Verwendung in Therapie, Diagnose und Chromatographie
KR100621354B1 (ko) * 2005-06-14 2006-09-08 광주과학기술원 유비퀴틴을 이용한 아밀로이드-베타 펩티드의 제조방법
CA2633887C (en) * 2005-12-15 2015-12-22 Genentech, Inc. Methods and compositions for targeting polyubiquitin
WO2009009773A1 (en) * 2007-07-11 2009-01-15 The Johns Hopkins University Use of otubain enzyme to cleave lysine-48-linked polyubiquitin
AU2010332932B2 (en) * 2009-12-14 2013-01-17 Navigo Proteins Gmbh Modified ubiquitin proteins having a specific binding activity for the extradomain B of fibronectin
US20130065272A1 (en) * 2010-03-24 2013-03-14 Medical Research Council Synthesis of Site Specifically-Linked Ubiquitin
JP6198807B2 (ja) * 2012-03-16 2017-09-20 ジェネンテック， インコーポレイテッド 操作され立体構造的に安定化されたタンパク質
EP3027745A4 (en) * 2013-08-04 2017-01-25 Yeda Research and Development Co., Ltd. Artificial cellulosomes comprising multiple scaffolds and uses thereof in biomass degradation
TWI569816B (zh) 2014-04-18 2017-02-11 林信湧 一種用於治療痛風之吸入式醫藥組成物及其備製方法
KR20170025040A (ko) * 2015-08-27 2017-03-08 한국기초과학지원연구원 염 용리 가능한 k48 폴리-유비퀴틴 사슬 특이 친화성 컬럼 및 이의 용도

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014090721A (ja) 2012-10-31 2014-05-19 Samsung Electronics Co Ltd 二重特異性（ｂｉｓｐｅｃｉｆｉｃ）抗原結合蛋白質複合体及び二重特異性抗体の製造方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Qian, S. B. et al.，Fusion proteins with COOH-terminal ubiquitin are stable and maintain dual functionality in vivo，J. Biol. Chem.，2002年，Vol. 277(41)，pp. 38818-38826
坂田 絵理 ほか，細胞の中の不要なタンパク質に目印をつける仕組み，日本結晶学会誌，Vol. 52，2010年，pp. 255-261
岩井 一宏，直鎖状ポリユビキチン鎖の発見とその機能，生化学，2012年，Vol. 84(11)，pp. 920-930

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US20210347827A1 (en) 2021-11-11
KR20190139841A (ko) 2019-12-18
JP2021525807A (ja) 2021-09-27
EP3805256A4 (en) 2022-03-23
WO2019231208A1 (ko) 2019-12-05
AU2019276622A1 (en) 2020-12-24
CN112204046A (zh) 2021-01-08
KR20190135393A (ko) 2019-12-06
EP3805256A1 (en) 2021-04-14
AU2023204533A1 (en) 2023-08-17
KR102155982B1 (ko) 2020-09-15
CA3101185A1 (en) 2019-12-05

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