JP7281423B2 - oral composition - Google Patents

Description

The present invention relates to oral compositions.

Conventionally, in order to prevent oral diseases such as dental caries, periodontal disease and bad breath, it is effective to inhibit the formation of biofilms in the oral cavity and to remove already formed biofilms. Oral compositions have been developed using a variety of ingredients such as foaming agents, bactericidal agents, and the like.

Among these components, erythritol is an effective component for suppressing or dissociating the agglutination reaction between bacteria in the oral cavity, as described in Patent Document 1, for example, and has only an inhibitory effect on biofilm formation. In addition, it is known to be excellent in the effect of removing biofilms formed in gaps between teeth and periodontal pockets. The dentifrice composition described in Patent Document 2 further enhances the biofilm removal effect in the oral cavity by increasing the erythritol content.

JP 2005-29484 A JP 2012-36172 A

However, when using a high content of erythritol, which is excellent in biofilm formation inhibitory effect and removal effect in the oral cavity as in the above patent document, dentin hypersensitivity is caused by osmotic pressure stimulation caused by dissolution of erythritol in water. It has been found by the inventor that the resulting pain is enhanced.
In this way, it is possible to realize an oral composition that can effectively prevent pain caused by dentin hypersensitivity while having a high content of erythritol, which has excellent effects of inhibiting and removing intraoral biofilms. This is strongly desired.

That is, the present invention relates to an oral composition that can effectively suppress the enhancement of pain caused by dentin hypersensitivity due to a high content of erythritol.

Therefore, as a result of various studies, the present inventors have made erythritol a factor by using sodium fluoride and calcium glycerophosphate in combination with a high content of erythritol and a specific mass ratio of erythritol and water. It is possible to effectively reduce the osmotic pressure stimulus and efficiently form a fluoride film such as calcium fluoride that effectively blocks the opening of the dentin tubule on the surface of the exposed dentin. It has been found that an oral composition can be obtained that can effectively prevent pain caused by texture hypersensitivity from being increased.

Accordingly, the present invention provides the following components (A), (B), (C) and (D):
(A) Erythritol 6% by mass or more and 33% by mass or less (B) Sodium fluoride (C) Calcium glycerophosphate (D) Water is contained, and the mass ratio of the content of component (A) to the content of component (D) Provided is an oral composition in which ((A)/(D)) is 0.06 or more and 0.2 or less.

According to the oral cavity composition of the present invention, although the erythritol content is high, the osmotic stimulation caused by erythritol is reduced, and the openings of dentin tubules on the exposed dentin surface are treated with fluoride. can be efficiently and effectively blocked by the coating, it is possible to sufficiently exhibit the excellent pain suppressing effect caused by dentine hypersensitivity. Therefore, with a high content of erythritol, it is possible to fully enjoy an excellent intraoral biofilm formation inhibitory effect and removal effect.

FIG. 1 is an electron micrograph of the surface of dentin when the composition for oral cavity of Example 3 was used. FIG. 2 is an electron micrograph of the surface of dentin when the composition for oral cavity of Comparative Example 2 was used.

The present invention will be described in detail below.
In the present invention, "dentin tubule" is also referred to as "dentin tubule". In addition, "to block dentinal tubules" means to physically block dentinal tubules, and not only the state of covering the opening of dentinal tubules and the periphery of the opening on the surface of dentin, but also the state near the surface of dentinal tubules. The term also includes the state of filling and capping (stopping) a capillary.
Further, in the present invention, the term "fluoride" means a fluorine-containing inorganic compound such as calcium fluoride mainly formed by the component (B) and the component (C), and the film of fluoride is referred to as the "fluoride film ” is also called.

The oral cavity composition of the present invention contains 6% by mass or more and 33% by mass or less of erythritol as the component (A). As a result, the formation of a fluoride coating by components (B) and (C), which will be described later, is promoted, and the coating efficiently and physically blocks dentinal tubules, resulting in a pain suppression effect caused by dentin hypersensitivity. can be demonstrated. In addition, erythritol can inhibit the agglutination reaction between bacteria or dissociate the agglutination between bacteria, thereby exhibiting the biofilm formation inhibitory effect and biofilm removal effect, which are the inherent effects of erythritol.

The content of the component (A) is the oral cavity of the present invention, from the viewpoint of sufficiently exhibiting the film formation promoting effect of the components (B) and (C) while maintaining the biofilm formation inhibitory effect and removal effect. 6% by mass or more, preferably 8% by mass or more, more preferably 10% by mass or more, still more preferably 12% by mass or more, and even more preferably 14% by mass in the composition for That's it. In addition, from the viewpoint of effectively avoiding excessive enhancement of pain caused by dentine hypersensitivity due to osmotic stimulation, the content of component (A) in the oral composition of the present invention is It is 33% by mass or less, preferably 30% by mass or less, more preferably 25% by mass or less, even more preferably 20% by mass or less, and even more preferably 18% by mass or less. The content of component (A) in the oral composition of the present invention is 6% by mass or more and 33% by mass or less, preferably 8 to 30% by mass, more preferably 10 to 25% by mass. %, more preferably 12 to 20% by mass, and even more preferably 14 to 18% by mass.

The oral composition of the present invention contains sodium fluoride as component (B). As a result, while ensuring the biofilm formation inhibitory effect and removal effect of the component (A), the film formation promoting effect of the component (A) is exhibited after the oral composition of the present invention is applied to the oral cavity. Fluoride ions are rapidly released during this process, and a fluoride film can be efficiently formed on the surface of dentin together with the component (C) described later. Therefore, the dentin tubules are effectively blocked by such a coating, and the osmotic stimulation caused by the high content of the component (A) effectively prevents the pain caused by dentine hypersensitivity from being enhanced. can be suppressed to

The content of component (B) in the oral composition of the present invention is preferably 0.1% by mass or more, more preferably 0.2% by mass, from the viewpoint of efficiently forming a fluoride coating. or more, more preferably 0.3% by mass or more, and even more preferably 0.5% by mass or more. The content of component (B) in the oral composition of the present invention is preferably 2.5% by mass or less, more preferably 2.2% by mass, from the viewpoint of ensuring the storage stability of the composition. % or less, more preferably 2% by mass or less, and even more preferably 1.8% by mass or less. The content of component (B) in the oral composition of the present invention is preferably 0.1% by mass or more and 2.5% by mass or less, more preferably 0.2 to 2.2% by mass. , more preferably 0.3 to 2% by mass, and even more preferably 0.5 to 1.8% by mass.

The oral composition of the present invention contains calcium glycerophosphate as component (C). Such component (C) can form a film while efficiently forming fluoride in combination with component (B) under the effect of promoting film formation by component (A), and greatly contributes to the sealing of dentinal tubules. becomes possible.

The content of component (C) is preferably 0 in the oral composition of the present invention from the viewpoint of effectively and efficiently forming a fluoride film together with component (B) in the presence of component (A). 0.1% by mass or more, more preferably 0.3% by mass or more, still more preferably 0.5% by mass or more, and even more preferably 1% by mass or more. The content of component (C) is preferably 5% by mass or less, more preferably 4.5% by mass or less, in the oral composition of the present invention from the viewpoint of ensuring the storage stability of the composition. , more preferably 4% by mass or less, and even more preferably 3.5% by mass or less. The content of component (C) in the oral composition of the present invention is preferably 0.1% by mass or more and 5% by mass or less, more preferably 0.3 to 4.5% by mass. , more preferably 0.5 to 4% by mass, and even more preferably 1 to 3.5% by mass.

The mass ratio (Ca/F) between the calcium atom equivalent amount of component (C) and the fluorine atom equivalent amount of component (B) is preferably 0.35 or more from the viewpoint of promoting fluoride film formation, It is more preferably 0.38 or more, and still more preferably 0.42 or more. Further, the mass ratio (Ca/F) between the amount of component (C) in terms of calcium atoms and the amount of component (B) in terms of fluorine atoms is preferably 0.55 or less, more preferably 0.52 or less, More preferably, it is 0.5 or less. The mass ratio (Ca/F) between the calcium atom equivalent amount of component (C) and the fluorine atom equivalent amount of component (B) is preferably 0.35 or more and 0.55 or less, more preferably 0.55 or less. 38 to 0.52, more preferably 0.42 to 0.5.

The value of the ratio ((Ca/F)/(A ), unit: mass % ⁻¹ ) is preferably 0.015 or more from the viewpoint of effectively avoiding excessive enhancement of pain caused by dentin hypersensitivity due to osmotic stimulation, It is more preferably 0.017 or more, and still more preferably 0.02 or more. Also, the value of the ratio of the mass ratio (Ca/F) between the amount of component (C) in terms of calcium atoms and the amount of fluorine atoms in terms of components (B) with respect to the content of component (A) ((Ca/F)/( A), unit: mass % ⁻¹ ) is preferable from the viewpoint of sufficiently exhibiting the effect of promoting film formation by the component (B) and the component (C) while maintaining the biofilm formation inhibitory effect and removal effect. is 0.15 or less, more preferably 0.1 or less, and still more preferably 0.07 or less. Then, the value of the ratio ((Ca/F)/ (A), unit: mass % ⁻¹ ) is preferably 0.015 or more and 0.15 or less, more preferably 0.017 to 0.1, still more preferably 0.020 to 0.07 be.

The mass ratio of the content of component (C) to the content of component (A) ((C)/(A)) is from the viewpoint of fully utilizing the excellent effect of promoting the formation of a fluoride film by component (A). , preferably 0.02 or more, more preferably 0.05 or more, still more preferably 0.1 or more, preferably 0.3 or less, more preferably 0.28 or less, and further It is preferably 0.24 or less, and more preferably 0.18 or less. The mass ratio ((C)/(A)) of the content of component (C) to the content of component (A) is preferably 0.02 or more and 0.3 or less, more preferably 0.02 or more and 0.3 or less. 05 to 0.28, more preferably 0.1 to 0.24, even more preferably 0.1 to 0.18.

The oral composition of the present invention contains water as component (D). By adjusting the content of water, it is possible to effectively avoid the enhancement of pain caused by dentine hypersensitivity due to osmotic stimulation caused by the high content of component (A). . In addition, the fluorine ions released from the component (B) are present in the composition, and the component (A) is sufficiently distributed in the oral cavity to form a fluoride film with the component (B) and the component (C). Accelerated, it is possible to seal the dentinal tubules quickly and efficiently with such a coating.

The content of component (D) is from the viewpoint of suppressing osmotic pressure stimulation caused by the high content of component (A), and from the viewpoint of efficiently forming a fluoride coating with component (B) and component (C). From the viewpoint of improving the blocking effect of dentinal tubules by forming the component (A), and from the viewpoint of effectively exhibiting the biofilm formation inhibitory effect and the biofilm removal effect by the component (A), the oral composition of the present invention preferably contains 55% by mass. or more, more preferably 60% by mass or more, still more preferably 65% by mass or more, still more preferably 68% by mass or more, preferably 93% by mass or less, more preferably 91% by mass or less, more preferably 89% by mass or less, even more preferably 88% by mass or less, still more preferably 87% by mass or less, and even more preferably 86.7% by mass or less. The content of component (D) in the oral composition of the present invention is preferably 55% by mass or more and 93% by mass or less, more preferably 60 to 91% by mass, and still more preferably 65 to 93% by mass. 89% by mass, more preferably 68 to 88% by mass, even more preferably 68 to 87% by mass, and even more preferably 68 to 86.7% by mass.

The mass ratio of the content of component (A) to the content of component (D) ((A)/(D)) ensures an excellent effect of promoting fluoride film formation by component (A), while maintaining component ( A) is 0.06 or more, preferably 0.08, from the viewpoint of moderately controlling the osmotic pressure stimulus caused by the high content of A) and improving the sealing effect of the dentinal tubules by the fluoride coating. or more, more preferably 0.09 or more, still more preferably 0.1 or more, and 0.3 or less, preferably 0.26 or less, more preferably 0.24 or less , and more preferably 0.22 or less. The mass ratio ((A)/(D)) between the content of component (A) and the content of component (D) is 0.06 or more and 0.3 or less, preferably 0.08 to It is 0.26, more preferably 0.09 to 0.24, still more preferably 0.1 to 0.22.

The oral composition of the present invention limits the content of an anionic surfactant from the viewpoint of effectively and efficiently forming a fluoride coating with component (B) and component (C) in the presence of component (A). preferably. Examples of such anionic surfactants include sodium lauryl sulfate, sodium lauroylmethyltaurate, and the like. The content of the anionic surfactant in the oral composition of the present invention is preferably less than 1% by mass, more preferably less than 0.6% by mass, and even more preferably less than 0.4% by mass. , still more preferably less than 0.2% by weight, even more preferably less than 0.08% by weight, or the oral compositions of the present invention are substantially free of anionic surfactants. preferable.

The composition for oral use of the present invention effectively and efficiently forms a fluoride film with the component (B) and the component (C) in the presence of the component (A), thereby exhibiting the effect of sealing the dentinal tubules by the fluoride film. From the viewpoint of improvement, it is preferable to limit the total content of fluorine compounds other than component (B) and polyvalent metal salts. Specific examples of fluorine compounds other than the component (B) include fluorine ion supplying compounds such as potassium fluoride and ammonium fluoride, and fluorine compounds such as sodium monofluorophosphate. Further, specific examples of polyvalent metal salts include one or more salts selected from copper, iron, calcium, magnesium, aluminum, zinc, and tin. The total content of fluorine compounds other than component (B) and polyvalent metal salts in the oral composition of the present invention is preferably less than 0.02% by mass, more preferably 0.015% by mass or less. and more preferably 0.005% by mass or less, or the oral composition of the present invention preferably does not contain fluorine compounds and polyvalent metal salts other than component (B).

The oral composition of the present invention may contain no abrasive, or may contain an abrasive within the limits of the total content of fluorine compounds other than component (B) and polyvalent metal salts. Such abrasives include abrasive silica (oil absorption of 50 to 150 mL/100 g, according to JIS K5101-13-2 (established in 2004), depending on the amount of boiled linseed oil absorbed), dicalcium phosphate dihydrate. one or two or more selected from abrasives such as phosphate and anhydride, calcium pyrophosphate, calcium carbonate, alumina, aluminum hydroxide, magnesium acetate, dimagnesium phosphate, magnesium acetate, dimagnesium phosphate, zeolite, etc. is mentioned.

In addition to the above ingredients, the oral composition of the present invention contains cationic surfactants, amphoteric surfactants such as coconut oil fatty acid amidopropyl betaine, polyoxyethylene hydrogenated castor oil and soap, within the range that does not inhibit the effects of the present invention. Surfactants such as nonionic surfactants such as sugar fatty acid esters and sorbitan fatty acid esters; polyhydric alcohols such as glycerin, propylene glycol and polyethylene glycol; sodium alginate, sodium carboxymethylcellulose, carrageenan, xanthan gum, sodium polyacrylate, Binders such as hydroxyethyl cellulose, hydroxypropyl cellulose, pectin, tragacanth gum, gum arabic, and guar gum; thickening silica; sweeteners such as sodium saccharin; flavors;

The pH of the oral cavity composition of the present invention at 25° C. is preferably 5.0 from the viewpoint of efficiently promoting the formation of a fluoride film by the component (B) and the component (C) in the presence of the component (A). or more, more preferably 6.0 or more, still more preferably 6.5 or more, and even more preferably 6.7 or more. In addition, the pH at 25° C. of the oral cavity composition of the present invention is preferably 10.0 or less, more preferably 9.6 or less, and still more preferably 9.0 or less, from the viewpoint of avoiding discoloration of the composition. It is 3 or less, and more preferably 8.8 or less.
The pH of the oral composition of the present invention is a value measured at 25°C using a pH electrode. When the oral composition of the present invention is a toothpaste composition, it is measured after adjusting to an aqueous solution with a concentration of 10% by mass with ion-exchanged water or purified water consisting of distilled water. value.

The oral composition of the present invention can efficiently and effectively prevent pain caused by dentin hypersensitivity with a fluoride coating. It can also be widely used as a hypersensitivity-reducing or prophylactic agent.

The method for producing the oral cavity composition of the present invention is not particularly limited, and the above ingredients may be appropriately mixed by a conventional method.

In relation to the above-described embodiments, the present invention further discloses the following oral compositions.
[1] The following components (A), (B), (C) and (D):
(A) Erythritol 6% by mass or more and 33% by mass or less (B) Sodium fluoride (C) Calcium glycerophosphate (D) Water is contained, and the mass ratio of the content of component (A) to the content of component (D) A composition for oral cavity, wherein ((A)/(D)) is 0.06 or more and 0.2 or less.
[2] The content of component (A) is preferably 8% by mass or more, more preferably 10% by mass or more, still more preferably 12% by mass or more, and even more preferably 14% by mass or more. preferably 30% by mass or less, more preferably 25% by mass or less, even more preferably 20% by mass or less, and even more preferably 18% by mass or less. .
[3] The content of component (B) is preferably 0.1% by mass or more, more preferably 0.2% by mass or more, still more preferably 0.3% by mass or more, and even more preferably is 0.5% by mass or more, preferably 2.5% by mass or less, more preferably 2.2% by mass or less, even more preferably 2% by mass or less, and even more preferably 1.8 The composition for oral cavity according to the above [1] or [2], which is not more than % by mass.
[4] The content of component (C) is preferably 0.1% by mass or more, more preferably 0.3% by mass or more, still more preferably 0.5% by mass or more, and even more preferably is 1% by mass or more, preferably 5% by mass or less, more preferably 4.5% by mass or less, still more preferably 4% by mass or less, and even more preferably 3.5% by mass or less The oral composition according to any one of [1] to [3] above.
[5] The content of component (D) is preferably 55% by mass or more, more preferably 60% by mass or more, still more preferably 65% by mass or more, and even more preferably 68% by mass or more. , preferably 93% by mass or less, more preferably 91% by mass or less, still more preferably 89% by mass or less, still more preferably 88% by mass or less, and even more preferably 87% by mass or less and more preferably 86.7% by mass or less.

[6] The mass ratio ((A)/(D)) of the content of component (A) to the content of component (D) is preferably 0.08 or more, more preferably 0.09 or more. Any one of the above [1] to [5], which is more preferably 0.1 or more, preferably 0.26 or less, more preferably 0.24 or less, and still more preferably 0.22 or less 1 oral composition.
[7] The content of the anionic surfactant is preferably less than 1% by mass, more preferably less than 0.6% by mass, still more preferably less than 0.4% by mass, still more preferably 0 The oral composition according to any one of [1] to [6] above, which contains less than 0.2% by mass, more preferably less than 0.08% by mass, or contains substantially no anionic surfactant.
[8] The mass ratio (Ca/F) between the calcium atom equivalent amount of component (C) and the fluorine atom equivalent amount of component (B) is preferably 0.35 or more, more preferably 0.38 or more. Any one of the above [1] to [7], which is more preferably 0.42 or more, preferably 0.55 or less, more preferably 0.52 or less, and still more preferably 0.5 or less 1 oral composition.
[9] The total content of fluorine compounds other than component (B) and polyvalent metal salts is preferably less than 0.02% by mass, more preferably 0.015% by mass or less, still more preferably 0.015% by mass or less. 005% by mass or less, or containing no fluoride or polyvalent metal salt other than component (B).
[10] The mass ratio of the content of component (C) to the content of component (A) ((C)/(A)) is preferably 0.02 or more, more preferably 0.05 or more. , more preferably 0.1 or more, preferably 0.3 or less, more preferably 0.28 or less, still more preferably 0.24 or less, and even more preferably 0.18 or less The oral composition according to any one of [1] to [9] above.
[11] The ratio of the mass ratio (Ca/F) between the calcium atom equivalent amount of component (C) and the fluorine atom equivalent amount of component (B) with respect to the content of component (A) ((Ca/F) /(A), unit: % by mass) is preferably 0.015 or ^more , more preferably 0.017 or more, still more preferably 0.02 or more, and preferably 0.15 or less. , more preferably 0.1 or less, and still more preferably 0.07 or less.
[12] The pH at 25°C is preferably 5.0 or higher, more preferably 6.0 or higher, still more preferably 6.5 or higher, still more preferably 6.7 or higher, preferably is 10.0 or less, more preferably 9.6 or less, still more preferably 9.3 or less, and even more preferably 8.8 or less. Any one of the above [1] to [11] Oral composition.

EXAMPLES The present invention will be specifically described below based on examples. In addition, unless otherwise indicated in the table, the content of each component indicates % by mass.

[Examples 1 to 12, Comparative Examples 1 to 5]
The liquid toothpaste compositions of Examples 1 to 12 and Comparative Examples 1 to 5 shown in Table 1 were produced by mixing each component by a conventional method.
Next, using the obtained liquid toothpaste composition and the following dentin sample, the closed state of the opening of the dentinal tubules and the fluoride coating were observed by the methods described below, and hypersensitivity sensory evaluation and plaque formation suppression were performed. rate measurements were made.

Test 1: Evaluation of opening closure of dentinal tubules and formation of fluoride film <Preparation of dentin sample>
The surface of each approximately 1 cm section (approximately 500 μm thick) of bovine dentin was mirror-polished using sandpaper with an abrasive grain size of 40 μm and sandpaper with an abrasive grain size of 3 μm, and then subjected to ultrasonic treatment for 10 minutes. bottom. Next, etching treatment was performed with a 1% citric acid aqueous solution for 20 seconds, and ultrasonic treatment was performed again for 10 minutes to completely remove the smear layer, thereby exposing the openings of the dentinal tubules of the bovine dentin. .

《Electron microscope observation》
After the obtained dentin samples were immersed in 10 mL of each liquid toothpaste composition of Example 3 and Comparative Example 2 for 5 minutes, the samples were washed with distilled water for 10 seconds. After washing, platinum was deposited on the surface of the sufficiently dried sample, and the opening of the dentinal tubule on the surface was observed at a magnification of 2000 using an electron microscope (VE-7800, manufactured by KEYENCE, accelerating voltage 2 kV). , and confirmed the state of opening closure of dentinal tubules and the state of fluoride film formation.
The results are shown in Figures 1-2.

From the results of FIGS. 1 and 2, in Example 3, it was confirmed that the openings of the dentinal tubules on the surface of the sample were sufficiently blocked by being well covered with a fluoride coating, whereas in Comparative Example In 2, although deposits can be confirmed on the surface of the dentin, it was confirmed that the portions covering the openings of the dentinal tubules and the portions where the dentin was exposed were only scattered.

Test 2: Sensitive sensory evaluation Each liquid toothpaste composition was given to 3 panelists, with those who had toothache every day in the last month, or those who had 2-3 times a week as panelists. After rinsing for 30 seconds, cold water was held in the mouth, and pain caused by dentine hypersensitivity was evaluated according to the following criteria.
4: No staining 3: Little staining 2: Slight staining 1: Staining Table 1 shows the average values of the evaluation results of the three panelists.

Test 3: Measurement of plaque formation inhibition rate 1) Treatment of HAp substrate with each liquid toothpaste composition One side of an HAp substrate (manufactured by Cosmo Bio Co., Ltd., 1 cm square) was mirror-polished using 40 µm, 12 µm, and 3 µm polishing paper. After that, it was immersed in 1N HCl for 1 minute for acid demineralization treatment. After the treatment, the HAp plate was washed with deionized water, dried, placed in a 24-well plate, 1 mL of each liquid toothpaste composition obtained in Examples and Comparative Examples was added, and shaken for 5 minutes. Shaking was performed using a shaker (BioShake iQ (manufactured by Wakenby Tech)) at room temperature (25° C.) and 500 rpm. After that, each liquid toothpaste composition was sucked up, 1 mL of ion-exchanged water was added, and the mixture was shaken for 5 minutes, after which water was sucked off to obtain a substrate to be treated.
2) Collection of stimulated saliva Healthy males in their 20s and 30s were asked to chew gum pellets contained in dent buff strips (Oral Care Inc.), and the saliva accumulated in their mouths was collected each time. Saliva was collected in Falcon tubes by having them expectorate into the Falcon tubes. Since there are individual differences in bacteria in saliva, the plaque formation inhibition rate was measured for all examples and comparative examples using the saliva of one healthy male.

3) Preparation of Model Dental Plaque Next, the saliva collected in the Falcon tube in 2) was centrifuged at 3000 rpm/rt/10 min. Sucrose was added to the separated supernatant saliva to prepare a 5% by mass sucralose solution, which was then stirred using a stirring device (Voltex, manufactured by Nippon Genetics) to prepare a dental plaque model test solution.
4) Evaluation of plaque formation inhibition rate After adding 1 mL of the model plaque test solution prepared in 3) to the HAp substrate treated in 1), it was stored in a plastic case together with a CO ₂ pack. Under anaerobic conditions, the cells were cultured at 37°C for 24 hours.

5) CV Staining of Dental Plaque Using a vacuum pump, the model dental plaque test solution in the plate was sucked up, 1 mL of deionized water was added, and the plate was shaken for 5 minutes. Next, water was sucked up using a pump, and 750 μL of 0.1 mass % crystal violet (CV) solution was added and shaken for 15 minutes.
Further, the CV solution was sucked up with a pump, 1 mL of ion-exchanged water was added, and the mixture was shaken for 5 minutes, and this was repeated twice. Next, the water was sucked up with a pump, 500 μL of ethanol was added and pipetted, and then the extract was diluted 10-fold with deionized water.
6) Evaluation of Plaque Formation Suppression Rate The absorbance OD _{595 nm} of the resulting 10-fold diluted solution of ion-exchanged water was measured with a microplate recorder (wavelength variable absorbance microplate reader Sunrise Rainbow Thermo manufactured by TECAN).
In addition, based on the absorbance OD _{595 nm} (initial value) when a model dental plaque was prepared on an HAp plate after acid demineralization treatment without being treated with the obtained liquid toothpaste composition, the following formula Therefore, the plaque formation inhibition rate (%) was calculated.
In addition, it means that the higher the value of the obtained plaque formation inhibition rate, the higher the plaque formation inhibition effect.
Plaque formation inhibition rate (%) = 100 - {OD _{595 nm} of substrate treated with liquid toothpaste composition obtained above / OD _{595 nm} of untreated substrate} x 100
Table 1 shows the results.

Claims (8)

The following components (A), (B), (C) and (D):
(A) Erythritol 6% by mass or more and 33% by mass or less (B) Sodium fluoride (C) Calcium glycerophosphate 0.22% by mass or more and 3.5% by mass or less (D) Content of component (A) containing water and the content of component (D) ((A)/(D)) is 0.06 or more and 0.2 or less. 2. The oral composition according to claim 1, wherein the anionic surfactant content is less than 1% by mass. The oral cavity composition according to claim 1 or 2, wherein the content of component (D) is 55% by mass or more and 93% by mass or less. 4. Any one of claims 1 to 3, wherein the mass ratio (Ca/F) between the calcium atom equivalent amount of component (C) and the fluorine atom equivalent amount of component (B) is 0.35 or more and 0.55 or less. The oral composition according to . The oral composition according to any one of claims 1 to 4, wherein the total content of fluorine compounds other than component (B) and polyvalent metal salts is less than 0.02% by mass. The oral composition according to any one of claims 1 to 5, which has a pH of 5.0 or more and 10.0 or less at 25°C. The oral composition according to any one of claims 1 to 6, wherein the content of component (B) is 0.1% by mass or more and 2.5% by mass or less. The composition for oral cavity according to any one of claims 1 to 7 , which is for dentine hypersensitivity.

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