JP7259182B2 - in situ分子検出のためのハイブリダイゼーション連鎖反応法 - Google Patents
in situ分子検出のためのハイブリダイゼーション連鎖反応法 Download PDFInfo
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- JP7259182B2 JP7259182B2 JP2018555974A JP2018555974A JP7259182B2 JP 7259182 B2 JP7259182 B2 JP 7259182B2 JP 2018555974 A JP2018555974 A JP 2018555974A JP 2018555974 A JP2018555974 A JP 2018555974A JP 7259182 B2 JP7259182 B2 JP 7259182B2
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12Q1/6841—In situ hybridisation
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/16—Aptamers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2320/00—Applications; Uses
- C12N2320/10—Applications; Uses in screening processes
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662326959P | 2016-04-25 | 2016-04-25 | |
| US62/326,959 | 2016-04-25 | ||
| PCT/US2017/029333 WO2017189525A1 (en) | 2016-04-25 | 2017-04-25 | Hybridization chain reaction methods for in situ molecular detection |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019518434A JP2019518434A (ja) | 2019-07-04 |
| JP2019518434A5 JP2019518434A5 (enExample) | 2020-06-18 |
| JP7259182B2 true JP7259182B2 (ja) | 2023-04-18 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018555974A Active JP7259182B2 (ja) | 2016-04-25 | 2017-04-25 | in situ分子検出のためのハイブリダイゼーション連鎖反応法 |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US11718874B2 (enExample) |
| EP (2) | EP4613756A3 (enExample) |
| JP (1) | JP7259182B2 (enExample) |
| CN (2) | CN116200465A (enExample) |
| AU (2) | AU2017257624B2 (enExample) |
| CA (2) | CA3210120C (enExample) |
| WO (1) | WO2017189525A1 (enExample) |
Families Citing this family (109)
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| JP2014531908A (ja) | 2011-10-14 | 2014-12-04 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 構造アッセンブリによる配列決定 |
| ES2991004T3 (es) | 2011-12-22 | 2024-12-02 | Harvard College | Métodos para la detección de analitos |
| US10138509B2 (en) | 2013-03-12 | 2018-11-27 | President And Fellows Of Harvard College | Method for generating a three-dimensional nucleic acid containing matrix |
| WO2016007839A1 (en) | 2014-07-11 | 2016-01-14 | President And Fellows Of Harvard College | Methods for high-throughput labelling and detection of biological features in situ using microscopy |
| ES3014093T3 (en) | 2014-07-30 | 2025-04-16 | Harvard College | Method for determining nucleic acids |
| EP4119677B1 (en) | 2015-04-10 | 2023-06-28 | Spatial Transcriptomics AB | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| JP6882282B2 (ja) | 2015-11-03 | 2021-06-02 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 三次元核酸含有マトリックスの立体撮像のための方法と装置 |
| EP4613756A3 (en) | 2016-04-25 | 2025-11-12 | President And Fellows Of Harvard College | Hybridization chain reaction methods for in situ molecular detection |
| WO2017222453A1 (en) | 2016-06-21 | 2017-12-28 | Hauling Thomas | Nucleic acid sequencing |
| IL290679B2 (en) | 2016-07-05 | 2023-10-01 | California Inst Of Techn | Hybridization chain reaction based on a minimal initiator |
| CN109923216B (zh) | 2016-08-31 | 2024-08-02 | 哈佛学院董事及会员团体 | 将生物分子的检测组合到使用荧光原位测序的单个试验的方法 |
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